Restriction map for
pFN21AA0358
(Circular) (Six-base) MAPSORT of: pFN21AA0358.seq Check: 6579 from: 1 to: 9448
pFN21AA0358 9448 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3086 5651 6673 3086
Size: 2565 1022 5861
Fragments arranged by size:
5861 2565 1022
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 5510 7258 278
Size: 53 83 186 936 2731 1243 1748 2468
Fragments arranged by size:
2731 2468 1748 1243 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 2805 6791 1937
Size: 648 220 3986 4594
Fragments arranged by size:
4594 3986 648 220
Acc65I G'GTAC_C
Cuts at: 6057 6769 6057
Size: 712 8736
AclI AA'CG_TT
Cuts at: 1563 7575 7948 1563
Size: 6012 373 3063
Fragments arranged by size:
6012 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4570 4713 4859 5445 5610 5850 6677 7511
Size: 2938 143 146 586 165 240 827 834
Cuts at: 7511 8895 9163 1632
Size: 1384 268 1917
Fragments arranged by size:
2938 1917 1384 834 827 586 268 240
165 146 143
AfeI AGC'GCT
Cuts at: 6434 6434
Size: 9448
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9251
AflIII A'CryG_T
Cuts at: 1203 3533 4399 8347 9324 1203
Size: 2330 866 3948 977 1327
Fragments arranged by size:
3948 2330 1327 977 866
AgeI A'CCGG_T
Cuts at: 8279 8279
Size: 9448
AhdI GACnn_n'nnGTC
Cuts at: 3400 6468 8177 3400
Size: 3068 1709 4671
Fragments arranged by size:
4671 3068 1709
AleI CACnn'nnGTG
Cuts at: 1166 2879 5603 1166
Size: 1713 2724 5011
Fragments arranged by size:
5011 2724 1713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5097 1184
Size: 3913 5535
AlwNI CAG_nnn'CTG
Cuts at: 3103 4837 6655 6730 7191 8763 3103
Size: 1734 1818 75 461 1572 3788
Fragments arranged by size:
3788 1818 1734 1572 461 75
ApaLI G'TGCA_C
Cuts at: 4850 7505 8661 4850
Size: 2655 1156 5637
Fragments arranged by size:
5637 2655 1156
ApoI r'AATT_y
Cuts at: 1493 3344 3419 3699 5347 6710 6757 6983 7135
Size: 1851 75 280 1648 1363 47 226 152
Cuts at: 7135 9244 1493
Size: 2109 1697
Fragments arranged by size:
2109 1851 1697 1648 1363 280 226 152
75 47
AseI AT'TA_AT
Cuts at: 160 1032 3954 8002 160
Size: 872 2922 4048 1606
Fragments arranged by size:
4048 2922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9448
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2177 2475 2649 2982 4482 4623
Size: 73 128 106 298 174 333 1500 141
Cuts at: 4623 4703 5283 5706 6773 1870
Size: 80 580 423 1067 4545
Fragments arranged by size:
4545 1500 1067 580 423 333 298 174
141 128 106 80 73
AvrII C'CTAG_G
Cuts at: 5869 5869
Size: 9448
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3520 3553 4561 4594 3520
Size: 33 1008 33 8374
Fragments arranged by size:
8374 1008 33 33
BamHI G'GATC_C
Cuts at: 3666 5215 5885 6778 7145 3666
Size: 1549 670 893 367 5969
Fragments arranged by size:
5969 1549 893 670 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2067 4619 5287 6057 6195
Size: 325 217 627 280 2552 668 770 138
Cuts at: 6195 6769 8224 618
Size: 574 1455 1842
Fragments arranged by size:
2552 1842 1455 770 668 627 574 325
280 217 138
BanII G_rGCy'C
Cuts at: 1391 2411 2832 4329 4420 6767 1391
Size: 1020 421 1497 91 2347 4072
Fragments arranged by size:
4072 2347 1497 1020 421 91
BbeI G_GCGC'C
Cuts at: 6199 6199
Size: 9448
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2230 6665 928
Size: 971 331 4435 3711
Fragments arranged by size:
4435 3711 971 331
BbvCI CC'TCA_GC
Cuts at: 3810 5063 3810
Size: 1253 8195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2717 2751 7639 7673 1903
Size: 34 780 34 4888 34 3678
Fragments arranged by size:
4888 3678 780 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7340 8550 7340
Size: 1210 8238
BclI T'GATC_A
Cuts at: 1548 5527 6256 1548
Size: 3979 729 4740
Fragments arranged by size:
4740 3979 729
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2628 8059 136
Size: 107 122 71 631 605 956 5431 1525
Fragments arranged by size:
5431 1525 956 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9443 9443
Size: 9448
BlpI GC'TnA_GC
Cuts at: 2543 6865 2543
Size: 4322 5126
Bme1580I G_kGCm'C
Cuts at: 1790 2722 4624 4854 5290 5714 7509 8665 1790
Size: 932 1902 230 436 424 1795 1156 2573
Fragments arranged by size:
2573 1902 1795 1156 932 436 424 230
BmgBI CAC'GTC
Cuts at: 1318 2529 3599 1318
Size: 1211 1070 7167
Fragments arranged by size:
7167 1211 1070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2648 2771 4007
Size: 416 53 83 399 24 1231 123 1236
Cuts at: 4007 4641 5198 5285 7179 8137 442
Size: 634 557 87 1894 958 1753
Fragments arranged by size:
1894 1753 1236 1231 958 634 557 416
399 123 87 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 3856 4495 5758 5870 2437
Size: 1419 639 1263 112 6015
Fragments arranged by size:
6015 1419 1263 639 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2816 2918 3359 4087 4934 5640 8108
Size: 1044 533 102 441 728 847 706 2468
Cuts at: 8108 1239
Size: 2579
Fragments arranged by size:
2579 2468 1044 847 728 706 533 441
102
Bpu10I CC'TnA_GC
Cuts at: 2189 3810 4943 5063 2189
Size: 1621 1133 120 6574
Fragments arranged by size:
6574 1621 1133 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 4465 5033 5560 5668 7573 8438 8736 2576
Size: 1889 568 527 108 1905 865 298 3288
Fragments arranged by size:
3288 1905 1889 865 568 527 298 108
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5618 5729 5915 6509 8111 882
Size: 617 4119 111 186 594 1602 2219
Fragments arranged by size:
4119 2219 1602 617 594 186 111
BsaAI yAC'GTr
Cuts at: 493 1204 7239 493
Size: 711 6035 2702
Fragments arranged by size:
6035 2702 711
BsaBI GATnn'nnATC
Cuts at: 6105 9442 6105
Size: 3337 6111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2925 4206 4264 5507
Size: 53 83 186 936 1392 1281 58 1243
Cuts at: 5507 6196 7255 7637 8272 275
Size: 689 1059 382 635 1451
Fragments arranged by size:
1451 1392 1281 1243 1059 936 689 635
382 186 83 58 53
BsaWI w'CCGG_w
Cuts at: 6081 7881 8279 8553 8700 9111 6081
Size: 1800 398 274 147 411 6418
Fragments arranged by size:
6418 1800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3830 3860 5456 5486 6125
Size: 30 165 30 2427 30 1596 30 639
Cuts at: 6125 6155 1178
Size: 30 4471
Fragments arranged by size:
4471 2427 1596 639 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2463 2720 3901 5036 5477 1186
Size: 1277 257 1181 1135 441 5157
Fragments arranged by size:
5157 1277 1181 1135 441 257
BseYI C'CCAG_C
Cuts at: 3321 3801 4133 4235 5228 6519 8651 9147 3321
Size: 480 332 102 993 1291 2132 496 3622
Fragments arranged by size:
3622 2132 1291 993 496 480 332 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 4985 6113 6668 1163
Size: 573 1143 2106 1128 555 3943
Fragments arranged by size:
3943 2106 1143 1128 573 555
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6898 7659 8687 1540
Size: 275 186 4897 761 1028 2301
Fragments arranged by size:
4897 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2411 2544 2832 2964 4854 7509 7594 8665 2411
Size: 133 288 132 1890 2655 85 1071 3194
Fragments arranged by size:
3194 2655 1890 1071 288 133 132 85
BsmI GAATG_Cn'
Cuts at: 2949 3017 6959 7052 2949
Size: 68 3942 93 5345
Fragments arranged by size:
5345 3942 93 68
BsmBI CGTCTCn'nnnn_
Cuts at: 2791 4213 4513 2791
Size: 1422 300 7726
Fragments arranged by size:
7726 1422 300
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2411 2544 2722 2832 2964 4329 4420
Size: 399 621 133 178 110 132 1365 91
Cuts at: 4420 4624 4854 5290 5714 6767 7509 7594 8665
Size: 204 230 436 424 1053 742 85 1071
Cuts at: 8665 1391
Size: 2174
Fragments arranged by size:
2174 1365 1071 1053 742 621 436 424
399 230 204 178 133 132 110 91
85
BspEI T'CCGG_A
Cuts at: 9111 9111
Size: 9448
BspHI T'CATG_A
Cuts at: 3349 7337 9067 3349
Size: 3988 1730 3730
Fragments arranged by size:
3988 3730 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2954 3086 3405 4174 5651 6673 6803 844
Size: 2110 132 319 769 1477 1022 130 3489
Fragments arranged by size:
3489 2110 1477 1022 769 319 132 130
BsrBI CCG'CTC
Cuts at: 1394 1823 2301 2508 3835 7335 1394
Size: 429 478 207 1327 3500 3507
Fragments arranged by size:
3507 3500 1327 478 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 3033 5662 7943 8117 65
Size: 1183 1785 2629 2281 174 1396
Fragments arranged by size:
2629 2281 1785 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4296 4697 4905 6309 8092 8279 1700
Size: 110 2486 401 208 1404 1783 187 2869
Fragments arranged by size:
2869 2486 1783 1404 401 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 5025 5054 6228 96
Size: 4929 29 1174 3316
Fragments arranged by size:
4929 3316 1174 29
BssHII G'CGCG_C
Cuts at: 1925 6586 1925
Size: 4661 4787
BssSI C'ACGA_G
Cuts at: 7508 8520 7508
Size: 1012 8436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4858 5294 794
Size: 4064 436 4948
Fragments arranged by size:
4948 4064 436
BstBI TT'CG_AA
Cuts at: 8252 8252
Size: 9448
BstEII G'GTnAC_C
Cuts at: 1184 6685 1184
Size: 5501 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 3082 4015 4877 3082
Size: 933 862 7653
Fragments arranged by size:
7653 933 862
BstYI r'GATC_y
Cuts at: 1972 2679 2754 3196 3363 3666 5215 5885 6778
Size: 707 75 442 167 303 1549 670 893
Cuts at: 6778 7145 7530 7547 8999 9085 9097 9443 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1549 1452 893 707 670 442 385
367 346 303 167 86 75 17 12
BstZ17I GTA'TAC
Cuts at: 2586 2586
Size: 9448
Bsu36I CC'TnA_GG
Cuts at: 4347 5313 4347
Size: 966 8482
BtgI C'CryG_G
Cuts at: 513 1065 2004 2353 3480 4147 5601 513
Size: 552 939 349 1127 667 1454 4360
Fragments arranged by size:
4360 1454 1127 939 667 552 349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6402 487
Size: 683 56 5176 3533
Fragments arranged by size:
5176 3533 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2891 3877 4094 4604 5692 6964 7758 7778
Size: 808 986 217 510 1088 1272 794 20
Cuts at: 7778 2083
Size: 3753
Fragments arranged by size:
3753 1272 1088 986 808 794 510 217
20
ClaI AT'CG_AT
Cuts at: 1554 2761 1554
Size: 1207 8241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9413
DraI TTT'AAA
Cuts at: 3288 5337 6752 3288
Size: 2049 1415 5984
Fragments arranged by size:
5984 2049 1415
DraIII CAC_nnn'GTG
Cuts at: 2673 2968 4858 5294 2673
Size: 295 1890 436 6827
Fragments arranged by size:
6827 1890 436 295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 2813 8455 809
Size: 507 948 549 5642 1802
Fragments arranged by size:
5642 1802 948 549 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3300 3630 4867
Size: 54 1425 50 275 18 1470 330 1237
Cuts at: 4867 5106 6117 6895 7784 9413 8
Size: 239 1011 778 889 1629 43
Fragments arranged by size:
1629 1470 1425 1237 1011 889 778 330
275 239 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6895 1537
Size: 275 5083 4090
Fragments arranged by size:
5083 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2385 2859 3907 4047 5168 7378 1353
Size: 1032 474 1048 140 1121 2210 3423
Fragments arranged by size:
3423 2210 1121 1048 1032 474 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2692 3490 8035 8409 8555 387
Size: 1064 138 1103 798 4545 374 146 1280
Fragments arranged by size:
4545 1280 1103 1064 798 374 146 138
EcoICRI GAG'CTC
Cuts at: 2409 2830 2409
Size: 421 9027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2816 2918 3359 4087 4570 4713
Size: 393 651 533 102 441 728 483 143
Cuts at: 4713 4859 4934 5445 5610 5640 5850 6677 7511
Size: 146 75 511 165 30 210 827 834
Cuts at: 7511 8108 8895 9163 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 834 827 787 728 651 597 533
511 483 441 393 268 210 165 146
143 102 75 30
EcoNI CCTnn'n_nnAGG
Cuts at: 4841 6680 4841
Size: 1839 7609
EcoO109I rG'GnC_Cy
Cuts at: 2349 2815 3814 5239 5279 5309 6035 6652 2349
Size: 466 999 1425 40 30 726 617 5145
Fragments arranged by size:
5145 1425 999 726 617 466 40 30
EcoRI G'AATT_C
Cuts at: 3419 3699 6757 7135 3419
Size: 280 3058 378 5732
Fragments arranged by size:
5732 3058 378 280
EcoRV GAT'ATC
Cuts at: 6367 6367
Size: 9448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3313 3921 3313
Size: 608 8840
FspI TGC'GCA
Cuts at: 7954 7954
Size: 9448
HaeII r_GCGC'y
Cuts at: 1678 2599 2623 6091 6199 6436 8595 1678
Size: 921 24 3468 108 237 2159 2531
Fragments arranged by size:
3468 2531 2159 921 237 108 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2265 2297 2696
Size: 32 135 32 641 32 20 32 399
Cuts at: 2696 2728 2793 2825 2836 2868 3386 3418 3830
Size: 32 65 32 11 32 518 32 412
Cuts at: 3830 3862 3884 3916 4457 4489 4730 4762 4923
Size: 32 22 32 541 32 241 32 161
Cuts at: 4923 4955 5456 5488 5512 5519 5544 5551 5870
Size: 32 501 32 24 7 25 7 319
Cuts at: 5870 5902 6270 6302 6454 6486 8089 8121 8163
Size: 32 368 32 152 32 1603 32 42
Cuts at: 8163 8195 1373
Size: 32 2626
Fragments arranged by size:
2626 1603 641 541 518 501 412 399
368 319 241 161 152 135 65 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 25 24 22 20 11
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2290 6792 7038 669
Size: 988 281 352 4502 246 3079
Fragments arranged by size:
4502 3079 988 352 281 246
HindIII A'AGCT_T
Cuts at: 748 5403 748
Size: 4655 4793
HpaI GTT'AAC
Cuts at: 2290 7038 2290
Size: 4748 4700
KasI G'GCGC_C
Cuts at: 6195 6195
Size: 9448
KpnI G_GTAC'C
Cuts at: 6061 6773 6061
Size: 712 8736
MfeI C'AATT_G
Cuts at: 7047 7047
Size: 9448
MluI A'CGCG_T
Cuts at: 3533 9324 3533
Size: 5791 3657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3137 4536 4997 5207 5985 8562 8746 1305
Size: 1832 1399 461 210 778 2577 184 2007
Fragments arranged by size:
2577 2007 1832 1399 778 461 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3302 3632 5108 6119 10
Size: 54 1425 343 1470 330 1476 1011 3339
Fragments arranged by size:
3339 1476 1470 1425 1011 343 330 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3008 3080 4404 5546 5603 6380
Size: 648 1713 129 72 1324 1142 57 777
Cuts at: 6380 6458 7406 7765 7924 518
Size: 78 948 359 159 2042
Fragments arranged by size:
2042 1713 1324 1142 948 777 648 359
159 129 78 72 57
MspA1I CmG'CkG
Cuts at: 1606 2203 3067 3100 3763 3805 4927 5558 6262
Size: 597 864 33 663 42 1122 631 704
Cuts at: 6262 6864 7541 8689 8934 1606
Size: 602 677 1148 245 2120
Fragments arranged by size:
2120 1148 1122 864 704 677 663 631
602 597 245 42 33
NaeI GCC'GGC
Cuts at: 4298 4699 4907 6311 4298
Size: 401 208 1404 7435
Fragments arranged by size:
7435 1404 401 208
NarI GG'CG_CC
Cuts at: 6196 6196
Size: 9448
NcoI C'CATG_G
Cuts at: 513 1065 2004 3480 513
Size: 552 939 1476 6481
Fragments arranged by size:
6481 1476 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9448
NgoMIV G'CCGG_C
Cuts at: 4296 4697 4905 6309 4296
Size: 401 208 1404 7435
Fragments arranged by size:
7435 1404 401 208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9448
NotI GC'GGCC_GC
Cuts at: 6895 6895
Size: 9448
NspI r_CATG'y
Cuts at: 1132 1229 3992 4403 6151 6344 6806 8351 1132
Size: 97 2763 411 1748 193 462 1545 2229
Fragments arranged by size:
2763 2229 1748 1545 462 411 193 97
PasI CC'CwG_GG
Cuts at: 1348 4798 1348
Size: 3450 5998
PciI A'CATG_T
Cuts at: 4399 8347 4399
Size: 3948 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9448
PfoI T'CCnGG_A
Cuts at: 4998 4998
Size: 9448
PmeI GTTT'AAAC
Cuts at: 6752 6752
Size: 9448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7517 7549 9030 9062 7517
Size: 32 1481 32 7903
Fragments arranged by size:
7903 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2349 2815 5239 5279 5309 6035 6652 2349
Size: 466 2424 40 30 726 617 5145
Fragments arranged by size:
5145 2424 726 617 466 40 30
PshAI GACnn'nnGTC
Cuts at: 1613 2928 4263 6033 1613
Size: 1315 1335 1770 5028
Fragments arranged by size:
5028 1770 1335 1315
PsiI TTA'TAA
Cuts at: 7018 7018
Size: 9448
PspXI vC'TCGA_Gb
Cuts at: 2649 2649
Size: 9448
PstI C_TGCA'G
Cuts at: 830 2433 3402 3516 3768 4035 6684 6800 830
Size: 1603 969 114 252 267 2649 116 3478
Fragments arranged by size:
3478 2649 1603 969 267 252 116 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9448
PvuII CAG'CTG
Cuts at: 3100 3763 3805 4927 5558 6262 3100
Size: 663 42 1122 631 704 6286
Fragments arranged by size:
6286 1122 704 663 631 42
SacI G_AGCT'C
Cuts at: 2411 2832 2411
Size: 421 9027
SalI G'TCGA_C
Cuts at: 1936 6790 1936
Size: 4854 4594
SanDI GG'GwC_CC
Cuts at: 2349 5239 6035 2349
Size: 2890 796 5762
Fragments arranged by size:
5762 2890 796
SapI GCTCTTCn'nnn_
Cuts at: 2859 3907 4047 2859
Size: 1048 140 8260
Fragments arranged by size:
8260 1048 140
SbfI CC_TGCA'GG
Cuts at: 6800 6800
Size: 9448
ScaI AGT'ACT
Cuts at: 2521 3653 7696 9408 2521
Size: 1132 4043 1712 2561
Fragments arranged by size:
4043 2561 1712 1132
SexAI A'CCwGG_T
Cuts at: 5671 5671
Size: 9448
SfcI C'TryA_G
Cuts at: 826 1045 2429 2611 2975 3398 3512 3764 4031
Size: 219 1384 182 364 423 114 252 267
Cuts at: 4031 5149 6680 6796 7931 8612 8803 826
Size: 1118 1531 116 1135 681 191 1471
Fragments arranged by size:
1531 1471 1384 1135 1118 681 423 364
267 252 219 191 182 116 114
SfoI GGC'GCC
Cuts at: 6197 6197
Size: 9448
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9448
SgrAI Cr'CCGG_yG
Cuts at: 1700 4296 1700
Size: 2596 6852
SmaI CCC'GGG
Cuts at: 1872 4705 6775 1872
Size: 2833 2070 4545
Fragments arranged by size:
4545 2833 2070
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 2649 4444 5048 5575 5647
Size: 197 926 648 58 1795 604 527 72
Cuts at: 5647 7552 8453 8715 820
Size: 1905 901 262 1553
Fragments arranged by size:
1905 1795 1553 926 901 648 604 527
262 197 72 58
SnaBI TAC'GTA
Cuts at: 493 7239 493
Size: 6746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9448
SphI G_CATG'C
Cuts at: 1132 6806 1132
Size: 5674 3774
SspI AAT'ATT
Cuts at: 5 52 7372 5
Size: 47 7320 2081
Fragments arranged by size:
7320 2081 47
StuI AGG'CCT
Cuts at: 1517 2750 3296 3385 5422 1517
Size: 1233 546 89 2037 5543
Fragments arranged by size:
5543 2037 1233 546 89
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2493 2904 3297 3480 3916
Size: 552 939 117 372 411 393 183 436
Cuts at: 3916 5449 5629 5869 6067 6887 513
Size: 1533 180 240 198 820 3074
Fragments arranged by size:
3074 1533 939 820 552 436 411 393
372 240 198 183 180 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 5087 6620 7475 7492 7645 7830
Size: 1255 1755 1752 1533 855 17 153 185
Cuts at: 7830 8275 325
Size: 445 1498
Fragments arranged by size:
1755 1752 1533 1498 1255 855 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2519 3651 5025
Size: 274 80 33 51 1443 542 1132 1374
Cuts at: 5025 5054 6228 6558 7694 9406 96
Size: 29 1174 330 1136 1712 138
Fragments arranged by size:
1712 1443 1374 1174 1136 1132 542 330
274 138 80 51 33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2125 2157 2657 2689 9408 9440 2125
Size: 32 500 32 6719 32 2133
Fragments arranged by size:
6719 2133 500 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3926 5537 1316
Size: 2610 1611 5227
Fragments arranged by size:
5227 2610 1611
XbaI T'CTAG_A
Cuts at: 6784 6784
Size: 9448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3010 3334 3478 4403 3010
Size: 324 144 925 8055
Fragments arranged by size:
8055 925 324 144
XhoI C'TCGA_G
Cuts at: 1943 2649 1943
Size: 706 8742
XmaI C'CCGG_G
Cuts at: 1870 4703 6773 1870
Size: 2833 2070 4545
Fragments arranged by size:
4545 2833 2070
XmnI GAAnn'nnTTC
Cuts at: 2021 3385 7577 2021
Size: 1364 4192 3892
Fragments arranged by size:
4192 3892 1364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 5508 7256 276
Size: 53 83 186 936 2731 1243 1748 2468
Fragments arranged by size:
2731 2468 1748 1243 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BfrBI BsiWI FseI FspAI NruI
NsiI PacI PmlI PspOMI PsrI RsrII SacII SfiI
SrfI SwaI