(Circular) (Six-base) MAPSORT of: pFN21AA0348.seq Check: 4400 from: 1 to: 9193 pFN21AA0348 9193 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2887 5182 6048 6127 6457 2887 Size: 2295 866 79 330 5623 Fragments arranged by size: 5623 2295 866 330 79 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3471 7003 278 Size: 53 83 186 936 1935 3532 2468 Fragments arranged by size: 3532 2468 1935 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3471 6536 1937 Size: 1534 3065 4594 Fragments arranged by size: 4594 3065 1534 Acc65I G'GTAC_C Cuts at: 5998 6514 5998 Size: 516 8677 AclI AA'CG_TT Cuts at: 1563 7320 7693 1563 Size: 5757 373 3063 Fragments arranged by size: 5757 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2570 2780 2897 3395 3821 5358 6357 Size: 677 261 210 117 498 426 1537 999 Cuts at: 6357 7256 8640 8908 1632 Size: 899 1384 268 1917 Fragments arranged by size: 1917 1537 1384 999 899 677 498 426 268 261 210 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8996 AflIII A'CryG_T Cuts at: 1203 2181 8092 9069 1203 Size: 978 5911 977 1327 Fragments arranged by size: 5911 1327 978 977 AgeI A'CCGG_T Cuts at: 5348 8024 5348 Size: 2676 6517 AhdI GACnn_n'nnGTC Cuts at: 3561 5029 6358 7922 3561 Size: 1468 1329 1564 4832 Fragments arranged by size: 4832 1564 1468 1329 AleI CACnn'nnGTG Cuts at: 1166 2264 2664 1166 Size: 1098 400 7695 Fragments arranged by size: 7695 1098 400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2039 6135 1184 Size: 855 4096 4242 Fragments arranged by size: 4242 4096 855 AlwNI CAG_nnn'CTG Cuts at: 2285 4041 4810 6049 6936 8508 2285 Size: 1756 769 1239 887 1572 2970 Fragments arranged by size: 2970 1756 1572 1239 887 769 ApaI G_GGCC'C Cuts at: 4664 5805 4664 Size: 1141 8052 ApaLI G'TGCA_C Cuts at: 7250 8406 7250 Size: 1156 8037 ApoI r'AATT_y Cuts at: 1493 2274 3572 3580 4259 4296 5078 6502 6728 Size: 781 1298 8 679 37 782 1424 226 Cuts at: 6728 6880 8989 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1424 1298 782 781 679 226 152 37 8 AseI AT'TA_AT Cuts at: 160 1032 7747 160 Size: 872 6715 1606 Fragments arranged by size: 6715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9193 AvaI C'yCGr_G Cuts at: 1870 1943 2380 3209 3700 4623 5111 5847 6004 Size: 73 437 829 491 923 488 736 157 Cuts at: 6004 6518 1870 Size: 514 4545 Fragments arranged by size: 4545 923 829 736 514 491 488 437 157 73 BamHI G'GATC_C Cuts at: 3540 3591 6523 6890 3540 Size: 51 2932 367 5843 Fragments arranged by size: 5843 2932 367 51 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 4001 4523 5396 5570 Size: 325 217 627 250 1964 522 873 174 Cuts at: 5570 5671 5977 5998 6111 6128 6191 6514 7969 Size: 101 306 21 113 17 63 323 1455 Cuts at: 7969 618 Size: 1842 Fragments arranged by size: 1964 1842 1455 873 627 522 325 323 306 250 217 174 113 101 63 21 17 BanII G_rGCy'C Cuts at: 1391 3346 3388 4664 4749 5235 5637 5805 6305 Size: 1955 42 1276 85 486 402 168 500 Cuts at: 6305 6335 6512 1391 Size: 30 177 4072 Fragments arranged by size: 4072 1955 1276 500 486 402 177 168 85 42 30 BbeI G_GCGC'C Cuts at: 2041 4005 5675 5981 2041 Size: 1964 1670 306 5253 Fragments arranged by size: 5253 1964 1670 306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3096 3974 4104 4210 4907 5058 5067 Size: 971 1197 878 130 106 697 151 9 Cuts at: 5067 6127 928 Size: 1060 3994 Fragments arranged by size: 3994 1197 1060 971 878 697 151 130 106 9 BbvCI CC'TCA_GC Cuts at: 2204 2375 5451 2204 Size: 171 3076 5946 Fragments arranged by size: 5946 3076 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4601 4635 7384 7418 1903 Size: 34 2664 34 2749 34 3678 Fragments arranged by size: 3678 2749 2664 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7085 8295 7085 Size: 1210 7983 BclI T'GATC_A Cuts at: 1548 3000 4264 4564 1548 Size: 1452 1264 300 6177 Fragments arranged by size: 6177 1452 1264 300 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 2515 2985 Size: 107 122 71 631 605 457 386 470 Cuts at: 2985 5528 7804 136 Size: 2543 2276 1525 Fragments arranged by size: 2543 2276 1525 631 605 470 457 386 122 107 71 BglII A'GATC_T Cuts at: 9188 9188 Size: 9193 BlpI GC'TnA_GC Cuts at: 4360 6610 4360 Size: 2250 6943 Bme1580I G_kGCm'C Cuts at: 1790 2511 4528 4664 5805 5994 6114 6131 7254 Size: 721 2017 136 1141 189 120 17 1123 Cuts at: 7254 8410 1790 Size: 1156 2573 Fragments arranged by size: 2573 2017 1156 1141 1123 721 189 136 120 17 BmgBI CAC'GTC Cuts at: 1318 2226 1318 Size: 908 8285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3094 6350 6436 Size: 416 53 83 399 24 1677 3256 86 Cuts at: 6436 6924 7882 442 Size: 488 958 1753 Fragments arranged by size: 3256 1753 1677 958 488 416 399 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5141 5304 5141 Size: 163 9030 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2118 4476 5015 5993 6056 7853 1239 Size: 854 25 2358 539 978 63 1797 2579 Fragments arranged by size: 2579 2358 1797 978 854 539 63 25 Bpu10I CC'TnA_GC Cuts at: 2012 2204 2375 3317 3653 5451 2012 Size: 192 171 942 336 1798 5754 Fragments arranged by size: 5754 1798 942 336 192 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2533 3082 3215 5219 5752 7318 8183 8481 2533 Size: 549 133 2004 533 1566 865 298 3245 Fragments arranged by size: 3245 2004 1566 865 549 533 298 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2421 3047 3303 4521 5101 5292 5326 Size: 617 922 626 256 1218 580 191 34 Cuts at: 5326 5654 7856 882 Size: 328 2202 2219 Fragments arranged by size: 2219 2202 1218 922 626 617 580 328 256 191 34 BsaAI yAC'GTr Cuts at: 493 1204 2506 6984 493 Size: 711 1302 4478 2702 Fragments arranged by size: 4478 2702 1302 711 BsaBI GATnn'nnATC Cuts at: 9187 9187 Size: 9193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2038 3468 3897 4002 Size: 53 83 186 936 505 1430 429 105 Cuts at: 4002 5672 5978 7000 7382 8017 275 Size: 1670 306 1022 382 635 1451 Fragments arranged by size: 1670 1451 1430 1022 936 635 505 429 382 306 186 105 83 53 BsaWI w'CCGG_w Cuts at: 3594 3639 5348 5544 5758 7626 8024 8298 8445 Size: 45 1709 196 214 1868 398 274 147 Cuts at: 8445 8856 3594 Size: 411 3931 Fragments arranged by size: 3931 1868 1709 411 398 274 214 196 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2116 2146 2922 2952 4501 Size: 30 165 30 713 30 776 30 1549 Cuts at: 4501 4531 5943 5973 1178 Size: 30 1412 30 4398 Fragments arranged by size: 4398 1549 1412 776 713 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 2948 3494 4946 5148 5380 5580 6022 Size: 1171 591 546 1452 202 232 200 442 Cuts at: 6022 1186 Size: 4357 Fragments arranged by size: 4357 1452 1171 591 546 442 232 202 200 BseYI C'CCAG_C Cuts at: 2032 3693 3733 5162 8396 8892 2032 Size: 1661 40 1429 3234 496 2333 Fragments arranged by size: 3234 2333 1661 1429 496 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2242 3065 3212 1163 Size: 573 506 823 147 7144 Fragments arranged by size: 7144 823 573 506 147 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 4068 4587 6643 7404 8432 Size: 275 186 85 1982 519 2056 761 1028 Cuts at: 8432 1540 Size: 2301 Fragments arranged by size: 2301 2056 1982 1028 761 519 275 186 85 BsiHKAI G_wGCw'C Cuts at: 2943 3654 4624 4749 5235 5449 6305 7254 7339 Size: 711 970 125 486 214 856 949 85 Cuts at: 7339 8410 2943 Size: 1071 3726 Fragments arranged by size: 3726 1071 970 949 856 711 486 214 125 85 BsiWI C'GTAC_G Cuts at: 2184 2184 Size: 9193 BsmI GAATG_Cn' Cuts at: 6704 6797 6704 Size: 93 9100 BsmBI CGTCTCn'nnnn_ Cuts at: 2232 2626 5112 2232 Size: 394 2486 6313 Fragments arranged by size: 6313 2486 394 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2511 2943 3346 3388 3654 4528 4624 Size: 399 721 432 403 42 266 874 96 Cuts at: 4624 4664 4749 5235 5449 5637 5805 5994 6114 Size: 40 85 486 214 188 168 189 120 Cuts at: 6114 6131 6305 6335 6512 7254 7339 8410 1391 Size: 17 174 30 177 742 85 1071 2174 Fragments arranged by size: 2174 1071 874 742 721 486 432 403 399 266 214 189 188 177 174 168 120 96 85 85 42 40 30 17 BspEI T'CCGG_A Cuts at: 3594 3639 8856 3594 Size: 45 5217 3931 Fragments arranged by size: 5217 3931 45 BspHI T'CATG_A Cuts at: 4156 6403 7082 8812 4156 Size: 2247 679 1730 4537 Fragments arranged by size: 4537 2247 1730 679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2887 4743 5182 5274 6034 6048 6127 6457 Size: 2043 1856 439 92 760 14 79 330 Cuts at: 6457 6548 844 Size: 91 3489 Fragments arranged by size: 3489 2043 1856 760 439 330 92 91 79 14 BsrBI CCG'CTC Cuts at: 1394 1823 4090 7080 1394 Size: 429 2267 2990 3507 Fragments arranged by size: 3507 2990 2267 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7688 7862 65 Size: 1183 6440 174 1396 Fragments arranged by size: 6440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 5304 5348 7837 8024 1700 Size: 110 301 3193 44 2489 187 2869 Fragments arranged by size: 3193 2869 2489 301 187 110 44 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9193 BssHII G'CGCG_C Cuts at: 1925 2078 1925 Size: 153 9040 BssSI C'ACGA_G Cuts at: 7253 8265 7253 Size: 1012 8181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2869 6049 794 Size: 2075 3180 3938 Fragments arranged by size: 3938 3180 2075 BstBI TT'CG_AA Cuts at: 7997 7997 Size: 9193 BstEII G'GTnAC_C Cuts at: 1184 2569 1184 Size: 1385 7808 BstXI CCAn_nnnn'nTGG Cuts at: 2666 2701 5152 2666 Size: 35 2451 6707 Fragments arranged by size: 6707 2451 35 BstYI r'GATC_y Cuts at: 1972 3540 3591 6343 6523 6890 7275 7292 8744 Size: 1568 51 2752 180 367 385 17 1452 Cuts at: 8744 8830 8842 9188 1972 Size: 86 12 346 1977 Fragments arranged by size: 2752 1977 1568 1452 385 367 346 180 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 6418 6418 Size: 9193 BtgI C'CryG_G Cuts at: 513 1065 2004 2665 5401 5595 6123 513 Size: 552 939 661 2736 194 528 3583 Fragments arranged by size: 3583 2736 939 661 552 528 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3187 6201 6215 487 Size: 683 56 1961 3014 14 3465 Fragments arranged by size: 3465 3014 1961 683 56 14 BtsI GCAGTG_nn' Cuts at: 2301 4890 4974 6100 6461 6709 7503 7523 2301 Size: 2589 84 1126 361 248 794 20 3971 Fragments arranged by size: 3971 2589 1126 794 361 248 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9158 DraI TTT'AAA Cuts at: 6497 6497 Size: 9193 DraIII CAC_nnn'GTG Cuts at: 2527 2869 6430 2527 Size: 342 3561 5290 Fragments arranged by size: 5290 3561 342 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2432 4499 8200 809 Size: 507 1116 2067 3701 1802 Fragments arranged by size: 3701 2067 1802 1116 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2121 2402 Size: 54 1425 50 275 18 253 38 281 Cuts at: 2402 2683 3307 4065 5312 6640 7529 9158 8 Size: 281 624 758 1247 1328 889 1629 43 Fragments arranged by size: 1629 1425 1328 1247 889 758 624 281 281 275 253 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2083 4065 6640 1537 Size: 275 271 1982 2575 4090 Fragments arranged by size: 4090 2575 1982 275 271 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2328 3756 4704 7123 1353 Size: 972 3 1428 948 2419 3423 Fragments arranged by size: 3423 2419 1428 972 948 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2943 3677 5255 7780 8154 8300 Size: 1064 138 1354 734 1578 2525 374 146 Cuts at: 8300 387 Size: 1280 Fragments arranged by size: 2525 1578 1354 1280 1064 734 374 146 138 EcoICRI GAG'CTC Cuts at: 4747 5233 6303 4747 Size: 486 1070 7637 Fragments arranged by size: 7637 1070 486 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2118 2309 2570 2780 2897 3395 Size: 393 461 25 191 261 210 117 498 Cuts at: 3395 3821 4476 5015 5358 5993 6056 6357 7256 Size: 426 655 539 343 635 63 301 899 Cuts at: 7256 7853 8640 8908 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 899 787 655 635 597 539 498 461 426 393 343 301 268 261 210 191 117 63 25 EcoNI CCTnn'n_nnAGG Cuts at: 3320 5734 5946 3320 Size: 2414 212 6567 Fragments arranged by size: 6567 2414 212 EcoO109I rG'GnC_Cy Cuts at: 3214 3609 4660 4661 4732 5608 5802 5824 6046 Size: 395 1051 1 71 876 194 22 222 Cuts at: 6046 6076 6241 3214 Size: 30 165 6166 Fragments arranged by size: 6166 1051 876 395 222 194 165 71 30 22 1 EcoRI G'AATT_C Cuts at: 2274 3572 5078 6502 6880 2274 Size: 1298 1506 1424 378 4587 Fragments arranged by size: 4587 1506 1424 1298 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5505 5746 5505 Size: 241 8952 FspI TGC'GCA Cuts at: 7699 7699 Size: 9193 HaeII r_GCGC'y Cuts at: 1678 2041 3524 4005 5675 5981 6372 8340 1678 Size: 363 1483 481 1670 306 391 1968 2531 Fragments arranged by size: 2531 1968 1670 1483 481 391 363 306 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2479 2723 2755 3145 Size: 32 135 32 875 32 244 32 390 Cuts at: 3145 3177 3547 3551 3579 3583 4343 4375 4817 Size: 32 370 4 28 4 760 32 442 Cuts at: 4817 4849 4920 4952 5015 5047 5111 5143 5830 Size: 32 71 32 63 32 64 32 687 Cuts at: 5830 5862 6089 6121 6344 6376 7834 7866 7908 Size: 32 227 32 223 32 1458 32 42 Cuts at: 7908 7940 1373 Size: 32 2626 Fragments arranged by size: 2626 1458 875 760 687 442 390 370 244 227 223 135 71 64 63 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3265 3876 4492 6537 6783 669 Size: 988 281 1327 611 616 2045 246 3079 Fragments arranged by size: 3079 2045 1327 988 616 611 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9193 HpaI GTT'AAC Cuts at: 6783 6783 Size: 9193 KasI G'GCGC_C Cuts at: 2037 4001 5671 5977 2037 Size: 1964 1670 306 5253 Fragments arranged by size: 5253 1964 1670 306 KpnI G_GTAC'C Cuts at: 6002 6518 6002 Size: 516 8677 MfeI C'AATT_G Cuts at: 4776 6792 4776 Size: 2016 7177 MluI A'CGCG_T Cuts at: 2181 9069 2181 Size: 6888 2305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2718 3438 5225 5369 8307 8491 1305 Size: 1413 720 1787 144 2938 184 2007 Fragments arranged by size: 2938 2007 1787 1413 720 184 144 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2404 3309 10 Size: 54 1425 343 291 281 905 5894 Fragments arranged by size: 5894 1425 905 343 291 281 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2664 2996 4161 7151 7510 7669 Size: 648 1098 400 332 1165 2990 359 159 Cuts at: 7669 518 Size: 2042 Fragments arranged by size: 2990 2042 1165 1098 648 400 359 332 159 MspA1I CmG'CkG Cuts at: 1606 2497 2773 3737 3889 4454 6125 6365 6377 Size: 891 276 964 152 565 1671 240 12 Cuts at: 6377 6609 7286 8434 8679 1606 Size: 232 677 1148 245 2120 Fragments arranged by size: 2120 1671 1148 964 891 677 565 276 245 240 232 152 12 NaeI GCC'GGC Cuts at: 2113 2113 Size: 9193 NarI GG'CG_CC Cuts at: 2038 4002 5672 5978 2038 Size: 1964 1670 306 5253 Fragments arranged by size: 5253 1964 1670 306 NcoI C'CATG_G Cuts at: 513 1065 2004 5401 513 Size: 552 939 3397 4305 Fragments arranged by size: 4305 3397 939 552 NdeI CA'TA_TG Cuts at: 387 4049 387 Size: 3662 5531 NgoMIV G'CCGG_C Cuts at: 2111 2111 Size: 9193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9193 NotI GC'GGCC_GC Cuts at: 2083 6640 2083 Size: 4557 4636 NspI r_CATG'y Cuts at: 1132 1229 3505 6551 8096 1132 Size: 97 2276 3046 1545 2229 Fragments arranged by size: 3046 2276 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3238 1348 Size: 1890 7303 PciI A'CATG_T Cuts at: 8092 8092 Size: 9193 PflMI CCAn_nnn'nTGG Cuts at: 1108 2411 4911 6227 1108 Size: 1303 2500 1316 4074 Fragments arranged by size: 4074 2500 1316 1303 PfoI T'CCnGG_A Cuts at: 4638 4848 5553 4638 Size: 210 705 8278 Fragments arranged by size: 8278 705 210 PmeI GTTT'AAAC Cuts at: 6497 6497 Size: 9193 PmlI CAC'GTG Cuts at: 2506 2506 Size: 9193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2751 2783 7262 7294 8775 8807 2751 Size: 32 4479 32 1481 32 3137 Fragments arranged by size: 4479 3137 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3214 3609 4732 5608 6046 6241 3214 Size: 395 1123 876 438 195 6166 Fragments arranged by size: 6166 1123 876 438 395 195 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9193 PsiI TTA'TAA Cuts at: 5493 6763 5493 Size: 1270 7923 PspOMI G'GGCC_C Cuts at: 4660 5801 4660 Size: 1141 8052 PspXI vC'TCGA_Gb Cuts at: 3209 5847 3209 Size: 2638 6555 PstI C_TGCA'G Cuts at: 830 3228 4533 5899 6045 6054 6468 6545 830 Size: 2398 1305 1366 146 9 414 77 3478 Fragments arranged by size: 3478 2398 1366 1305 414 146 77 9 PvuI CG_AT'CG Cuts at: 2001 4587 2001 Size: 2586 6607 PvuII CAG'CTG Cuts at: 2497 3737 3889 4454 6365 6377 2497 Size: 1240 152 565 1911 12 5313 Fragments arranged by size: 5313 1911 1240 565 152 12 SacI G_AGCT'C Cuts at: 4749 5235 6305 4749 Size: 486 1070 7637 Fragments arranged by size: 7637 1070 486 SacII CC_GC'GG Cuts at: 6126 6126 Size: 9193 SalI G'TCGA_C Cuts at: 1936 6535 1936 Size: 4599 4594 SbfI CC_TGCA'GG Cuts at: 6045 6545 6045 Size: 500 8693 ScaI AGT'ACT Cuts at: 3805 7441 9153 3805 Size: 3636 1712 3845 Fragments arranged by size: 3845 3636 1712 SexAI A'CCwGG_T Cuts at: 2523 4511 5223 2523 Size: 1988 712 6493 Fragments arranged by size: 6493 1988 712 SfcI C'TryA_G Cuts at: 826 1045 2059 3224 4271 4277 4529 5895 6041 Size: 219 1014 1165 1047 6 252 1366 146 Cuts at: 6041 6050 6464 6541 7676 8357 8548 826 Size: 9 414 77 1135 681 191 1471 Fragments arranged by size: 1471 1366 1165 1135 1047 1014 681 414 252 219 191 146 77 9 6 SfoI GGC'GCC Cuts at: 2039 4003 5673 5979 2039 Size: 1964 1670 306 5253 Fragments arranged by size: 5253 1964 1670 306 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9193 SmaI CCC'GGG Cuts at: 1872 6520 1872 Size: 4648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2512 3061 3209 3230 5234 5731 Size: 197 926 569 549 148 21 2004 497 Cuts at: 5731 5847 7297 8198 8460 820 Size: 116 1450 901 262 1553 Fragments arranged by size: 2004 1553 1450 926 901 569 549 497 262 197 148 116 21 SnaBI TAC'GTA Cuts at: 493 6984 493 Size: 6491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9193 SphI G_CATG'C Cuts at: 1132 3505 6551 1132 Size: 2373 3046 3774 Fragments arranged by size: 3774 3046 2373 SspI AAT'ATT Cuts at: 5 52 3790 7117 5 Size: 47 3738 3327 2081 Fragments arranged by size: 3738 3327 2081 47 StuI AGG'CCT Cuts at: 1517 2612 2832 1517 Size: 1095 220 7878 Fragments arranged by size: 7878 1095 220 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2607 3360 4758 4798 4905 Size: 552 939 333 270 753 1398 40 107 Cuts at: 4905 5401 5463 5603 5994 6381 6632 513 Size: 496 62 140 391 387 251 3074 Fragments arranged by size: 3074 1398 939 753 552 496 391 387 333 270 251 140 107 62 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3192 4130 4607 4912 5284 5414 5716 Size: 1255 1612 938 477 305 372 130 302 Cuts at: 5716 6129 6418 7220 7237 7390 7575 8020 325 Size: 413 289 802 17 153 185 445 1498 Fragments arranged by size: 1612 1498 1255 938 802 477 445 413 372 305 302 289 185 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 7439 9151 Size: 274 80 33 51 1443 1826 3636 1712 Cuts at: 9151 96 Size: 138 Fragments arranged by size: 3636 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 2922 2954 4501 4533 5943 5975 9153 Size: 32 774 32 1547 32 1410 32 3178 Cuts at: 9153 9185 2116 Size: 32 2124 Fragments arranged by size: 3178 2124 1547 1410 774 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9193 XbaI T'CTAG_A Cuts at: 4471 4483 6529 4471 Size: 12 2046 7135 Fragments arranged by size: 7135 2046 12 XhoI C'TCGA_G Cuts at: 1943 3209 5847 1943 Size: 1266 2638 5289 Fragments arranged by size: 5289 2638 1266 XmaI C'CCGG_G Cuts at: 1870 6518 1870 Size: 4648 4545 XmnI GAAnn'nnTTC Cuts at: 2480 3834 7322 2480 Size: 1354 3488 4351 Fragments arranged by size: 4351 3488 1354 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3469 7001 276 Size: 53 83 186 936 1935 3532 2468 Fragments arranged by size: 3532 2468 1935 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SanDI SapI SfiI SrfI SwaI XcmI