Restriction map for pFN21AA0339
 (Circular) (Six-base) MAPSORT of: pFN21AA0339.seq  Check: 2085  from: 1  to: 9826



pFN21AA0339  9826 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   4260    5422    4260 
   Size:       1162    8664
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4813    7636     278 
   Size:         53      83     186     936    3277    2823    2468

  Fragments arranged by size: 

                3277    2823    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    7169    1937 
   Size:       5232    4594
 
Acc65I G'GTAC_C

Cuts at:   6647    7147    6647 
   Size:        500    9326
 
AclI AA'CG_TT

Cuts at:   1563    7125    7953    8326    1563 
   Size:       5562     828     373    3063

  Fragments arranged by size: 

                5562    3063     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2128    2303    2354    2940    3512    4589    5211    5971 
   Size:        496     175      51     586     572    1077     622     760

Cuts at:   5971    7889    9273    9541    1632 
   Size:       1918    1384     268    1917

  Fragments arranged by size: 

                1918    1917    1384    1077     760     622     586     572
                 496     268     175      51
 
AfeI AGC'GCT

Cuts at:   3215    3503    6842    3215 
   Size:        288    3339    6199

  Fragments arranged by size: 

                6199    3339     288
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    9629
 
AflIII A'CryG_T

Cuts at:   1203    2823    6540    8725    9702    1203 
   Size:       1620    3717    2185     977    1327

  Fragments arranged by size: 

                3717    2185    1620    1327     977
 
AgeI A'CCGG_T

Cuts at:   8657    8657 
   Size:       9826
 
AhdI GACnn_n'nnGTC

Cuts at:   8555    8555 
   Size:       9826
 
AleI CACnn'nnGTG

Cuts at:   1166    2145    2702    1166 
   Size:        979     557    8290

  Fragments arranged by size: 

                8290     979     557
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3500    5925    1184 
   Size:       2316    2425    5085

  Fragments arranged by size: 

                5085    2425    2316
 
AlwNI CAG_nnn'CTG

Cuts at:   2636    3005    3323    5276    6064    7569    9141    2636 
   Size:        369     318    1953     788    1505    1572    3321

  Fragments arranged by size: 

                3321    1953    1572    1505     788     369     318
 
ApaI G_GGCC'C

Cuts at:   3351    3618    4666    5424    6462    3351 
   Size:        267    1048     758    1038    6715

  Fragments arranged by size: 

                6715    1048    1038     758     267
 
ApaLI G'TGCA_C

Cuts at:   7883    9039    7883 
   Size:       1156    8670
 
ApoI r'AATT_y

Cuts at:   1493    4710    7135    7361    7513    9622    1493 
   Size:       3217    2425     226     152    2109    1697

  Fragments arranged by size: 

                3217    2425    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    8380     160 
   Size:        872    7348    1606

  Fragments arranged by size: 

                7348    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9826
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2041    2439    2623    2829    3248    3690    4297 
   Size:         73      98     398     184     206     419     442     607

Cuts at:   4297    5604    5669    5682    6020    6301    6462    7151    1870 
   Size:       1307      65      13     338     281     161     689    4545

  Fragments arranged by size: 

                4545    1307     689     607     442     419     398     338
                 281     206     184     161      98      73      65      13
            
 
AvrII C'CTAG_G

Cuts at:   5870    5870 
   Size:       9826
 
BamHI G'GATC_C

Cuts at:   2077    6732    7156    7523    2077 
   Size:       4655     424     367    4380

  Fragments arranged by size: 

                4655    4380     424     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2444    2693    2723    3592    3658 
   Size:        325     217     627     657     249      30     869      66

Cuts at:   3658    4370    5284    5530    6164    6201    6647    7147    8602 
   Size:        712     914     246     634      37     446     500    1455

Cuts at:   8602     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     914     869     712     657     634     627
                 500     446     325     249     246     217      66      37
                  30
 
BanII G_rGCy'C

Cuts at:   1391    3190    3255    3351    3618    3999    4018    4278    4666 
   Size:       1799      65      96     267     381      19     260     388

Cuts at:   4666    5424    5547    6051    6462    7118    7145    1391 
   Size:        758     123     504     411     656      27    4072

  Fragments arranged by size: 

                4072    1799     758     656     504     411     388     381
                 267     260     123      96      65      27      19
 
BbeI G_GCGC'C

Cuts at:   2448    6168    6205    2448 
   Size:       3720      37    6069

  Fragments arranged by size: 

                6069    3720      37
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2184    4565    4776    5037    5835    7077     928 
   Size:        971     285    2381     211     261     798    1242    3677

  Fragments arranged by size: 

                3677    2381    1242     971     798     285     261     211
            
 
BbvCI CC'TCA_GC

Cuts at:   3807    4111    4758    5230    6036    3807 
   Size:        304     647     472     806    7597

  Fragments arranged by size: 

                7597     806     647     472     304
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    6580    6614    8017    8051    1903 
   Size:         34    4643      34    1403      34    3678

  Fragments arranged by size: 

                4643    3678    1403      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4981    7718    8928    4981 
   Size:       2737    1210    5879

  Fragments arranged by size: 

                5879    2737    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       9826
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2978    3259    3296 
   Size:        107     122      71     631     605    1306     281      37

Cuts at:   3296    5780    8437     136 
   Size:       2484    2657    1525

  Fragments arranged by size: 

                2657    2484    1525    1306     631     605     281     122
                 107      71      37
 
BglII A'GATC_T

Cuts at:   3756    9821    3756 
   Size:       6065    3761
 
BlpI GC'TnA_GC

Cuts at:   3210    5739    5882    6481    6635    7243    3210 
   Size:       2529     143     599     154     608    5793

  Fragments arranged by size: 

                5793    2529     608     599     154     143
 
Bme1580I G_kGCm'C

Cuts at:   1790    2586    2711    3351    3447    3618    4619    4666    5265 
   Size:        796     125     640      96     171    1001      47     599

Cuts at:   5265    5424    5970    6462    6548    7887    9043    1790 
   Size:        159     546     492      86    1339    1156    2573

  Fragments arranged by size: 

                2573    1339    1156    1001     796     640     599     546
                 492     171     159     125      96      86      47
 
BmgBI CAC'GTC

Cuts at:   1318    2826    6541    1318 
   Size:       1508    3715    4603

  Fragments arranged by size: 

                4603    3715    1508
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2228    2598    4609 
   Size:        416      53      83     399      24     811     370    2011

Cuts at:   4609    5252    5309    5379    6406    6542    7557    8515     442 
   Size:        643      57      70    1027     136    1015     958    1753

  Fragments arranged by size: 

                2011    1753    1027    1015     958     811     643     416
                 399     370     136      83      70      57      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       9826
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3520    4939    5203    5473    5735    6148    3520 
   Size:       1419     264     270     262     413    7198

  Fragments arranged by size: 

                7198    1419     413     270     264     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2737    2851    3204    3300    3762    4818    5331    5535 
   Size:       1498     114     353      96     462    1056     513     204

Cuts at:   5535    5724    5918    6032    6587    8486    1239 
   Size:        189     194     114     555    1899    2579

  Fragments arranged by size: 

                2579    1899    1498    1056     555     513     462     353
                 204     194     189     114     114      96
 
Bpu10I CC'TnA_GC

Cuts at:   3807    4111    4758    5230    6036    3807 
   Size:        304     647     472     806    7597

  Fragments arranged by size: 

                7597     806     647     472     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   6677    7951    8816    9114    6677 
   Size:       1274     865     298    7389

  Fragments arranged by size: 

                7389    1274     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2318    3419    4422    8489     882 
   Size:        617     819    1101    1003    4067    2219

  Fragments arranged by size: 

                4067    2219    1101    1003     819     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    6342    6796    6847    7617     493 
   Size:        711    5138     454      51     770    2702

  Fragments arranged by size: 

                5138    2702     770     711     454      51
 
BsaBI GATnn'nnATC

Cuts at:   4968    6278    6785    7004    9820    4968 
   Size:       1310     507     219    2816    4974

  Fragments arranged by size: 

                4974    2816    1310     507     219
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2445    3800    3860    4810 
   Size:         53      83     186     936     912    1355      60     950

Cuts at:   4810    5342    6165    6202    7633    8015    8650     275 
   Size:        532     823      37    1431     382     635    1451

  Fragments arranged by size: 

                1451    1431    1355     950     936     912     823     635
                 532     382     186      83      60      53      37
 
BsaWI w'CCGG_w

Cuts at:   6171    6699    8259    8657    8931    9078    9489    6171 
   Size:        528    1560     398     274     147     411    6508

  Fragments arranged by size: 

                6508    1560     528     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2462    2492    3613    3643    6113 
   Size:         30     165      30    1059      30    1121      30    2470

Cuts at:   6113    6143    1178 
   Size:         30    4861

  Fragments arranged by size: 

                4861    2470    1121    1059     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3019    3037    3055    3357    3551    3554    3834    3837 
   Size:       1833      18      18     302     194       3     280       3

Cuts at:   3837    3893    3942    4276    4279    4319    4511    4559    4912 
   Size:         56      49     334       3      40     192      48     353

Cuts at:   4912    4916    4952    5116    5125    5128    5131    5134    5149 
   Size:          4      36     164       9       3       3       3      15

Cuts at:   5149    5176    5222    5387    5687    5700    5747    6059    6095 
   Size:         27      46     165     300      13      47     312      36

Cuts at:   6095    6107    6134    6137    6140    7052    1186 
   Size:         12      27       3       3     912    3960

  Fragments arranged by size: 

                3960    1833     912     353     334     312     302     300
                 280     194     192     165     164      56      49      48
                  47      46      40      36      36      27      27      18
                  18      15      13      12       9       4       3       3
                   3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:   2505    2686    3103    3254    3350    3388    3415    3453    3637 
   Size:        181     417     151      96      38      27      38     184

Cuts at:   3637    5417    5584    5995    9029    9525    2505 
   Size:       1780     167     411    3034     496    2806

  Fragments arranged by size: 

                3034    2806    1780     496     417     411     184     181
                 167     151      96      38      38      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2649    3604    6869    6934    1163 
   Size:        573     913     955    3265      65    4055

  Fragments arranged by size: 

                4055    3265     955     913     573      65
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2646    3772    7276    8037    9065    1540 
   Size:        275     186     645    1126    3504     761    1028    2301

  Fragments arranged by size: 

                3504    2301    1126    1028     761     645     275     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2427    3999    4389    5904    6471    7887    7972    9043    2427 
   Size:       1572     390    1515     567    1416      85    1071    3210

  Fragments arranged by size: 

                3210    1572    1515    1416    1071     567     390      85
            
 
BsiWI C'GTAC_G

Cuts at:   2393    6342    2393 
   Size:       3949    5877
 
BsmI GAATG_Cn'

Cuts at:   7337    7430    7337 
   Size:         93    9733
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2633    2832    3738    5349    2633 
   Size:        199     906    1611    7110

  Fragments arranged by size: 

                7110    1611     906     199
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2427    2586    2711    3190    3255    3351    3447 
   Size:        399     637     159     125     479      65      96      96

Cuts at:   3447    3618    3999    4018    4278    4389    4619    4666    5265 
   Size:        171     381      19     260     111     230      47     599

Cuts at:   5265    5424    5547    5904    5970    6051    6462    6471    6548 
   Size:        159     123     357      66      81     411       9      77

Cuts at:   6548    7118    7145    7887    7972    9043    1391 
   Size:        570      27     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     637     599     570     479     411
                 399     381     357     260     230     171     159     159
                 125     123     111      96      96      85      81      77
                  66      65      47      27      19       9
 
BspEI T'CCGG_A

Cuts at:   6171    6699    9489    6171 
   Size:        528    2790    6508

  Fragments arranged by size: 

                6508    2790     528
 
BspHI T'CATG_A

Cuts at:   7715    9445    7715 
   Size:       1730    8096
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3249    3901    4260    5422    5640    6339    6681    7181 
   Size:       2405     652     359    1162     218     699     342     500

Cuts at:   7181     844 
   Size:       3489

  Fragments arranged by size: 

                3489    2405    1162     699     652     500     359     342
                 218
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2661    3405    3672    4611    5212    5617    6189 
   Size:        429     838     744     267     939     601     405     572

Cuts at:   6189    6349    7713    1394 
   Size:        160    1364    3507

  Fragments arranged by size: 

                3507    1364     939     838     744     601     572     429
                 405     267     160
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    6765    8321    8495      65 
   Size:       1183    5517    1556     174    1396

  Fragments arranged by size: 

                5517    1556    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3258    3279    6612    8470    8657    1700 
   Size:        110    1448      21    3333    1858     187    2869

  Fragments arranged by size: 

                3333    2869    1858    1448     187     110      21
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       9826
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       9826
 
BssSI C'ACGA_G

Cuts at:   4592    6737    7886    8898    4592 
   Size:       2145    1149    1012    5520

  Fragments arranged by size: 

                5520    2145    1149    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2730    3005    5357    6064     794 
   Size:       1936     275    2352     707    4556

  Fragments arranged by size: 

                4556    2352    1936     707     275
 
BstBI TT'CG_AA

Cuts at:   8630    8630 
   Size:       9826
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       9826
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2704    4264    5527    7030    2704 
   Size:       1560    1263    1503    5500

  Fragments arranged by size: 

                5500    1560    1503    1263
 
BstYI r'GATC_y

Cuts at:   1972    2077    2376    3756    6732    7080    7156    7523    7908 
   Size:        105     299    1380    2976     348      76     367     385

Cuts at:   7908    7925    9377    9463    9475    9821    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                2976    1977    1452    1380     385     367     348     346
                 299     105      86      76      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2976    4374    5367    6756     513 
   Size:        552     939     972    1398     993    1389    3583

  Fragments arranged by size: 

                3583    1398    1389     993     972     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2968    6168     487 
   Size:        683      56    1742    3200    4145

  Fragments arranged by size: 

                4145    3200    1742     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2697    2994    3003    6265    6943    7342    8136    8156    2697 
   Size:        297       9    3262     678     399     794      20    4367

  Fragments arranged by size: 

                4367    3262     794     678     399     297      20       9
            
 
ClaI AT'CG_AT

Cuts at:   1554    6903    1554 
   Size:       5349    4477
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    9791
 
DraI TTT'AAA

Cuts at:   7130    7130 
   Size:       9826
 
DraIII CAC_nnn'GTG

Cuts at:   6260    6915    6260 
   Size:        655    9171
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5011    5765    6782    8833     809 
   Size:        507    3695     754    1017    2051    1802

  Fragments arranged by size: 

                3695    2051    1802    1017     754     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2643    2785    2979 
   Size:         54    1425      50     275      18     813     142     194

Cuts at:   2979    4524    5213    5772    5916    6721    6822    6927    7273 
   Size:       1545     689     559     144     805     101     105     346

Cuts at:   7273    8162    9791       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1545    1425     889     813     805     689     559
                 346     275     194     144     142     105     101      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2643    7273    1537 
   Size:        275     831    4630    4090

  Fragments arranged by size: 

                4630    4090     831     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2361    2432    2668    3028    3064    3540    3742    4406 
   Size:       1008      71     236     360      36     476     202     664

Cuts at:   4406    4836    5122    5173    5193    5199    5464    5724    5990 
   Size:        430     286      51      20       6     265     260     266

Cuts at:   5990    6084    6099    6111    7756    1353 
   Size:         94      15      12    1645    3423

  Fragments arranged by size: 

                3423    1645    1008     664     476     430     360     286
                 266     265     260     236     202      94      71      51
                  36      20      15      12       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2071    3007    3807    6056    6204    6643 
   Size:       1064     138     482     936     800    2249     148     439

Cuts at:   6643    8413    8787    8933     387 
   Size:       1770     374     146    1280

  Fragments arranged by size: 

                2249    1770    1280    1064     936     800     482     439
                 374     148     146     138
 
EcoICRI GAG'CTC

Cuts at:   3997    3997 
   Size:       9826
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2128    2303    2354    2737    2851    2940    3204 
   Size:        393     496     175      51     383     114      89     264

Cuts at:   3204    3300    3512    3762    4589    4818    5211    5331    5535 
   Size:         96     212     250     827     229     393     120     204

Cuts at:   5535    5724    5918    5971    6032    6587    7889    8486    9273 
   Size:        189     194      53      61     555    1302     597     787

Cuts at:   9273    9541    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1302     827     787     597     555     496     393
                 393     383     268     264     250     229     212     204
                 194     189     175     120     114      96      89      61
                  53      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3327    5723    3327 
   Size:       2396    7430
 
EcoO109I rG'GnC_Cy

Cuts at:   2038    2599    3347    3615    4131    4662    4663    5363    5421 
   Size:        561     748     268     516     531       1     700      58

Cuts at:   5421    5874    6007    6016    6459    2038 
   Size:        453     133       9     443    5405

  Fragments arranged by size: 

                5405     748     700     561     531     516     453     443
                 268     133      58       9       1
 
EcoRI G'AATT_C

Cuts at:   7135    7513    7135 
   Size:        378    9448
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3532    3532 
   Size:       9826
 
FspI TGC'GCA

Cuts at:   2102    8332    2102 
   Size:       6230    3596
 
HaeII r_GCGC'y

Cuts at:   1678    2448    3139    3217    3505    5869    6168    6205    6613 
   Size:        770     691      78     288    2364     299      37     408

Cuts at:   6613    6642    6844    8973    1678 
   Size:         29     202    2129    2531

  Fragments arranged by size: 

                2531    2364    2129     770     691     408     299     288
                 202      78      37      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2759    2791    3790    3822    4051 
   Size:         32     135      32    1187      32     999      32     229

Cuts at:   4051    4083    4726    4758    4892    4924    5078    5110    5633 
   Size:         32     643      32     134      32     154      32     523

Cuts at:   5633    5663    5665    5695    6113    6145    6599    6631    7072 
   Size:         30       2      30     418      32     454      32     441

Cuts at:   7072    7104    8467    8499    8541    8573    1373 
   Size:         32    1363      32      42      32    2626

  Fragments arranged by size: 

                2626    1363    1187     999     643     523     454     441
                 418     229     154     135     134      42      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2152    4065    7170    7416     669 
   Size:        988     281     214    1913    3105     246    3079

  Fragments arranged by size: 

                3105    3079    1913     988     281     246     214
 
HindIII A'AGCT_T

Cuts at:    748    2746     748 
   Size:       1998    7828
 
HpaI GTT'AAC

Cuts at:   4065    7416    4065 
   Size:       3351    6475
 
KasI G'GCGC_C

Cuts at:   2444    6164    6201    2444 
   Size:       3720      37    6069

  Fragments arranged by size: 

                6069    3720      37
 
KpnI G_GTAC'C

Cuts at:   6651    7151    6651 
   Size:        500    9326
 
MfeI C'AATT_G

Cuts at:   7425    7425 
   Size:       9826
 
MluI A'CGCG_T

Cuts at:   9702    9702 
   Size:       9826
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3786    4995    8940    9124    1305 
   Size:       2481    1209    3945     184    2007

  Fragments arranged by size: 

                3945    2481    2007    1209     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2787    6929      10 
   Size:         54    1425     343     955    4142    2907

  Fragments arranged by size: 

                4142    2907    1425     955     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2145    2702    3482    3972    4262    5525    5907 
   Size:        648     979     557     780     490     290    1263     382

Cuts at:   5907    7028    7784    8143    8302     518 
   Size:       1121     756     359     159    2042

  Fragments arranged by size: 

                2042    1263    1121     979     780     756     648     557
                 490     382     359     290     159
 
MspA1I CmG'CkG

Cuts at:   1606    2678    3209    4081    4115    5999    6076    6565    6625 
   Size:       1072     531     872      34    1884      77     489      60

Cuts at:   6625    7242    7919    9067    9312    1606 
   Size:        617     677    1148     245    2120

  Fragments arranged by size: 

                2120    1884    1148    1072     872     677     617     531
                 489     245      77      60      34
 
NaeI GCC'GGC

Cuts at:   3260    3260 
   Size:       9826
 
NarI GG'CG_CC

Cuts at:   2445    6165    6202    2445 
   Size:       3720      37    6069

  Fragments arranged by size: 

                6069    3720      37
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    6756     513 
   Size:        552     939    4752    3583

  Fragments arranged by size: 

                4752    3583     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       9826
 
NgoMIV G'CCGG_C

Cuts at:   3258    3258 
   Size:       9826
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       9826
 
NotI GC'GGCC_GC

Cuts at:   7273    7273 
   Size:       9826
 
NspI r_CATG'y

Cuts at:   1132    1229    6832    7184    8729    1132 
   Size:         97    5603     352    1545    2229

  Fragments arranged by size: 

                5603    2229    1545     352      97
 
PasI CC'CwG_GG

Cuts at:   1348    4323    4858    1348 
   Size:       2975     535    6316

  Fragments arranged by size: 

                6316    2975     535
 
PciI A'CATG_T

Cuts at:   8725    8725 
   Size:       9826
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3323    5417    1108 
   Size:       2215    2094    5517

  Fragments arranged by size: 

                5517    2215    2094
 
PfoI T'CCnGG_A

Cuts at:   4401    5895    6009    4401 
   Size:       1494     114    8218

  Fragments arranged by size: 

                8218    1494     114
 
PmeI GTTT'AAAC

Cuts at:   7130    7130 
   Size:       9826
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3354    3386    7895    7927    9408    9440    3354 
   Size:         32    4509      32    1481      32    3740

  Fragments arranged by size: 

                4509    3740    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   6007    6007 
   Size:       9826
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       9826
 
PsiI TTA'TAA

Cuts at:   7396    7396 
   Size:       9826
 
PspOMI G'GGCC_C

Cuts at:   3347    3614    4662    5420    6458    3347 
   Size:        267    1048     758    1038    6715

  Fragments arranged by size: 

                6715    1048    1038     758     267
 
PstI C_TGCA'G

Cuts at:    830    3001    5362    6069    6360    7178     830 
   Size:       2171    2361     707     291     818    3478

  Fragments arranged by size: 

                3478    2361    2171     818     707     291
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9826
 
PvuII CAG'CTG

Cuts at:   2678    3209    4081    4115    5999    6565    2678 
   Size:        531     872      34    1884     566    5939

  Fragments arranged by size: 

                5939    1884     872     566     531      34
 
RsrII CG'GwC_CG

Cuts at:   6615    6615 
   Size:       9826
 
SacI G_AGCT'C

Cuts at:   3999    3999 
   Size:       9826
 
SalI G'TCGA_C

Cuts at:   1936    7168    1936 
   Size:       5232    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2432    2668    3742    4406    4836    5122    2432 
   Size:        236    1074     664     430     286    7136

  Fragments arranged by size: 

                7136    1074     664     430     286     236
 
SbfI CC_TGCA'GG

Cuts at:   5362    7178    5362 
   Size:       1816    8010
 
ScaI AGT'ACT

Cuts at:   8074    9786    8074 
   Size:       1712    8114
 
SexAI A'CCwGG_T

Cuts at:   3322    3322 
   Size:       9826
 
SfcI C'TryA_G

Cuts at:    826    1045    2997    3663    5358    6065    6356    7174    8309 
   Size:        219    1952     666    1695     707     291     818    1135

Cuts at:   8309    8990    9181     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1952    1695    1471    1135     818     707     681     666
                 291     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   5780    5780 
   Size:       9826
 
SfoI GGC'GCC

Cuts at:   2446    6166    6203    2446 
   Size:       3720      37    6069

  Fragments arranged by size: 

                6069    3720      37
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9826
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       9826
 
SmaI CCC'GGG

Cuts at:   1872    3692    5606    6464    7153    1872 
   Size:       1820    1914     858     689    4545

  Fragments arranged by size: 

                4545    1914    1820     858     689
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    6692    7930    8831    9093     820 
   Size:        197     926    4749    1238     901     262    1553

  Fragments arranged by size: 

                4749    1553    1238     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    6342    7617     493 
   Size:       5849    1275    2702

  Fragments arranged by size: 

                5849    2702    1275
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9826
 
SphI G_CATG'C

Cuts at:   1132    7184    1132 
   Size:       6052    3774
 
SspI AAT'ATT

Cuts at:      5      52    7750       5 
   Size:         47    7698    2081

  Fragments arranged by size: 

                7698    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2107    4145    4244    4738    5356    1517 
   Size:        590    2038      99     494     618    5987

  Fragments arranged by size: 

                5987    2038     618     590     494      99
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2085    2448    2617    2809    3565    3702 
   Size:        552     939      81     363     169     192     756     137

Cuts at:   3702    3990    4539    5870    6756    6969    7265     513 
   Size:        288     549    1331     886     213     296    3074

  Fragments arranged by size: 

                3074    1331     939     886     756     552     549     363
                 296     288     213     192     169     137      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3925    4020    4336    5500    7853    7870    8023 
   Size:       1255    2345      95     316    1164    2353      17     153

Cuts at:   8023    8208    8653     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                2353    2345    1498    1255    1164     445     316     185
                 153      95      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    8072    9784      96 
   Size:        274      80      33      51    1443    6095    1712     138

  Fragments arranged by size: 

                6095    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2462    2494    3613    3645    4859    4891    9786    9818    2462 
   Size:         32    1119      32    1214      32    4895      32    2470

  Fragments arranged by size: 

                4895    2470    1214    1119      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       9826
 
XbaI T'CTAG_A

Cuts at:   7162    7162 
   Size:       9826
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       9826
 
XmaI C'CCGG_G

Cuts at:   1870    3690    5604    6462    7151    1870 
   Size:       1820    1914     858     689    4545

  Fragments arranged by size: 

                4545    1914    1820     858     689
 
XmnI GAAnn'nnTTC

Cuts at:   3369    7955    3369 
   Size:       4586    5240
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4811    7634     276 
   Size:         53      83     186     936    3277    2823    2468

  Fragments arranged by size: 

                3277    2823    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI     BaeI    BfrBI  BstZ17I   Bsu36I    EcoRV     FseI
    FspAI     NruI     NsiI     PacI     PmlI    PspXI     PsrI    SacII
    SanDI     SrfI     SwaI     XcmI
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