Restriction map for
pFN21AA0339
(Linear) (Six-base) MAPSORT of: KIAA0339.seq Check: 2441 from: 1 to: 5121
KIAA0339.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2255 3417 5121
Size: 2255 1162 1704
Fragments arranged by size:
2255 1704 1162
AatII G_ACGT'C
Cuts at: 0 2808 5121
Size: 2808 2313
Acc65I G'GTAC_C
Cuts at: 0 4642 5121
Size: 4642 479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 298 349 935 1507 2584 3206 3966
Size: 123 175 51 586 572 1077 622 760
Cuts at: 3966 5121
Size: 1155
Fragments arranged by size:
1155 1077 760 622 586 572 175 123
51
AfeI AGC'GCT
Cuts at: 0 1210 1498 4837 5121
Size: 1210 288 3339 284
Fragments arranged by size:
3339 1210 288 284
AflIII A'CryG_T
Cuts at: 0 818 4535 5121
Size: 818 3717 586
Fragments arranged by size:
3717 818 586
AleI CACnn'nnGTG
Cuts at: 0 140 697 5121
Size: 140 557 4424
Fragments arranged by size:
4424 557 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1495 3920 5121
Size: 1495 2425 1201
Fragments arranged by size:
2425 1495 1201
AlwNI CAG_nnn'CTG
Cuts at: 0 631 1000 1318 3271 4059 5121
Size: 631 369 318 1953 788 1062
Fragments arranged by size:
1953 1062 788 631 369 318
ApaI G_GGCC'C
Cuts at: 0 1346 1613 2661 3419 4457 5121
Size: 1346 267 1048 758 1038 664
Fragments arranged by size:
1346 1048 1038 758 664 267
ApoI r'AATT_y
Cuts at: 0 2705 5121
Size: 2705 2416
AvaI C'yCGr_G
Cuts at: 0 36 434 618 824 1243 1685 2292 3599
Size: 36 398 184 206 419 442 607 1307
Cuts at: 3599 3664 3677 4015 4296 4457 5121
Size: 65 13 338 281 161 664
Fragments arranged by size:
1307 664 607 442 419 398 338 281
206 184 161 65 36 13
AvrII C'CTAG_G
Cuts at: 0 3865 5121
Size: 3865 1256
BamHI G'GATC_C
Cuts at: 0 72 4727 5121
Size: 72 4655 394
Fragments arranged by size:
4655 394 72
BanI G'GyrC_C
Cuts at: 0 439 688 718 1587 1653 2365 3279 3525
Size: 439 249 30 869 66 712 914 246
Cuts at: 3525 4159 4196 4642 5121
Size: 634 37 446 479
Fragments arranged by size:
914 869 712 634 479 446 439 249
246 66 37 30
BanII G_rGCy'C
Cuts at: 0 1185 1250 1346 1613 1994 2013 2273 2661
Size: 1185 65 96 267 381 19 260 388
Cuts at: 2661 3419 3542 4046 4457 5113 5121
Size: 758 123 504 411 656 8
Fragments arranged by size:
1185 758 656 504 411 388 381 267
260 123 96 65 19 8
BbeI G_GCGC'C
Cuts at: 0 443 4163 4200 5121
Size: 443 3720 37 921
Fragments arranged by size:
3720 921 443 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 2560 2771 3032 3830 5072 5121
Size: 179 2381 211 261 798 1242 49
Fragments arranged by size:
2381 1242 798 261 211 179 49
BbvCI CC'TCA_GC
Cuts at: 0 1802 2106 2753 3225 4031 5121
Size: 1802 304 647 472 806 1090
Fragments arranged by size:
1802 1090 806 647 472 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4575 4609 5121
Size: 4575 34 512
Fragments arranged by size:
4575 512 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2976 5121
Size: 2976 2145
BglI GCCn_nnn'nGGC
Cuts at: 0 973 1254 1291 3775 5121
Size: 973 281 37 2484 1346
Fragments arranged by size:
2484 1346 973 281 37
BglII A'GATC_T
Cuts at: 0 1751 5121
Size: 1751 3370
BlpI GC'TnA_GC
Cuts at: 0 1205 3734 3877 4476 4630 5121
Size: 1205 2529 143 599 154 491
Fragments arranged by size:
2529 1205 599 491 154 143
Bme1580I G_kGCm'C
Cuts at: 0 581 706 1346 1442 1613 2614 2661 3260
Size: 581 125 640 96 171 1001 47 599
Cuts at: 3260 3419 3965 4457 4543 5121
Size: 159 546 492 86 578
Fragments arranged by size:
1001 640 599 581 578 546 492 171
159 125 96 86 47
BmgBI CAC'GTC
Cuts at: 0 821 4536 5121
Size: 821 3715 585
Fragments arranged by size:
3715 821 585
BmrI ACTGGGnnnn_n'
Cuts at: 0 223 593 2604 3247 3304 3374 4401 4537
Size: 223 370 2011 643 57 70 1027 136
Cuts at: 4537 5121
Size: 584
Fragments arranged by size:
2011 1027 643 584 370 223 136 70
57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1515 2934 3198 3468 3730 4143 5121
Size: 1515 1419 264 270 262 413 978
Fragments arranged by size:
1515 1419 978 413 270 264 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 846 1199 1295 1757 2813 3326 3530
Size: 732 114 353 96 462 1056 513 204
Cuts at: 3530 3719 3913 4027 4582 5121
Size: 189 194 114 555 539
Fragments arranged by size:
1056 732 555 539 513 462 353 204
194 189 114 114 96
Bpu10I CC'TnA_GC
Cuts at: 0 1802 2106 2753 3225 4031 5121
Size: 1802 304 647 472 806 1090
Fragments arranged by size:
1802 1090 806 647 472 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4672 5121
Size: 4672 449
BsaI GGTCTCn'nnnn_
Cuts at: 0 313 1414 2417 5121
Size: 313 1101 1003 2704
Fragments arranged by size:
2704 1101 1003 313
BsaAI yAC'GTr
Cuts at: 0 4337 4791 4842 5121
Size: 4337 454 51 279
Fragments arranged by size:
4337 454 279 51
BsaBI GATnn'nnATC
Cuts at: 0 2963 4273 4780 4999 5121
Size: 2963 1310 507 219 122
Fragments arranged by size:
2963 1310 507 219 122
BsaHI Gr'CG_yC
Cuts at: 0 440 1795 1855 2805 3337 4160 4197 5121
Size: 440 1355 60 950 532 823 37 924
Fragments arranged by size:
1355 950 924 823 532 440 60 37
BsaWI w'CCGG_w
Cuts at: 0 4166 4694 5121
Size: 4166 528 427
Fragments arranged by size:
4166 528 427
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 1608 1638 4108 4138 5121
Size: 457 30 1121 30 2470 30 983
Fragments arranged by size:
2470 1121 983 457 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1014 1032 1050 1352 1546 1549 1829 1832
Size: 1014 18 18 302 194 3 280 3
Cuts at: 1832 1888 1937 2271 2274 2314 2506 2554 2907
Size: 56 49 334 3 40 192 48 353
Cuts at: 2907 2911 2947 3111 3120 3123 3126 3129 3144
Size: 4 36 164 9 3 3 3 15
Cuts at: 3144 3171 3217 3382 3682 3695 3742 4054 4090
Size: 27 46 165 300 13 47 312 36
Cuts at: 4090 4102 4129 4132 4135 5047 5121
Size: 12 27 3 3 912 74
Fragments arranged by size:
1014 912 353 334 312 302 300 280
194 192 165 164 74 56 49 48
47 46 40 36 36 27 27 18
18 15 13 12 9 4 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 500 681 1098 1249 1345 1383 1410 1448
Size: 500 181 417 151 96 38 27 38
Cuts at: 1448 1632 3412 3579 3990 5121
Size: 184 1780 167 411 1131
Fragments arranged by size:
1780 1131 500 417 411 184 181 167
151 96 38 38 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1599 4864 4929 5121
Size: 644 955 3265 65 192
Fragments arranged by size:
3265 955 644 192 65
BsiEI CG_ry'CG
Cuts at: 0 641 1767 5121
Size: 641 1126 3354
Fragments arranged by size:
3354 1126 641
BsiHKAI G_wGCw'C
Cuts at: 0 422 1994 2384 3899 4466 5121
Size: 422 1572 390 1515 567 655
Fragments arranged by size:
1572 1515 655 567 422 390
BsiWI C'GTAC_G
Cuts at: 0 388 4337 5121
Size: 388 3949 784
Fragments arranged by size:
3949 784 388
BsmBI CGTCTCn'nnnn_
Cuts at: 0 628 827 1733 3344 5121
Size: 628 199 906 1611 1777
Fragments arranged by size:
1777 1611 906 628 199
Bsp1286I G_dGCh'C
Cuts at: 0 422 581 706 1185 1250 1346 1442 1613
Size: 422 159 125 479 65 96 96 171
Cuts at: 1613 1994 2013 2273 2384 2614 2661 3260 3419
Size: 381 19 260 111 230 47 599 159
Cuts at: 3419 3542 3899 3965 4046 4457 4466 4543 5113
Size: 123 357 66 81 411 9 77 570
Cuts at: 5113 5121
Size: 8
Fragments arranged by size:
599 570 479 422 411 381 357 260
230 171 159 159 125 123 111 96
96 81 77 66 65 47 19 9
8
BspEI T'CCGG_A
Cuts at: 0 4166 4694 5121
Size: 4166 528 427
Fragments arranged by size:
4166 528 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1244 1896 2255 3417 3635 4334 4676 5121
Size: 1244 652 359 1162 218 699 342 445
Fragments arranged by size:
1244 1162 699 652 445 359 342 218
BsrBI CCG'CTC
Cuts at: 0 656 1400 1667 2606 3207 3612 4184 4344
Size: 656 744 267 939 601 405 572 160
Cuts at: 4344 5121
Size: 777
Fragments arranged by size:
939 777 744 656 601 572 405 267
160
BsrDI GCAATG_nn'
Cuts at: 0 4760 5121
Size: 4760 361
BsrFI r'CCGG_y
Cuts at: 0 1253 1274 4607 5121
Size: 1253 21 3333 514
Fragments arranged by size:
3333 1253 514 21
BssSI C'ACGA_G
Cuts at: 0 2587 4732 5121
Size: 2587 2145 389
Fragments arranged by size:
2587 2145 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 725 1000 3352 4059 5121
Size: 725 275 2352 707 1062
Fragments arranged by size:
2352 1062 725 707 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 2259 3522 5025 5121
Size: 699 1560 1263 1503 96
Fragments arranged by size:
1560 1503 1263 699 96
BstYI r'GATC_y
Cuts at: 0 72 371 1751 4727 5075 5121
Size: 72 299 1380 2976 348 46
Fragments arranged by size:
2976 1380 348 299 72 46
BtgI C'CryG_G
Cuts at: 0 971 2369 3362 4751 5121
Size: 971 1398 993 1389 370
Fragments arranged by size:
1398 1389 993 971 370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 963 4163 5121
Size: 963 3200 958
Fragments arranged by size:
3200 963 958
BtsI GCAGTG_nn'
Cuts at: 0 692 989 998 4260 4938 5121
Size: 692 297 9 3262 678 183
Fragments arranged by size:
3262 692 678 297 183 9
ClaI AT'CG_AT
Cuts at: 0 4898 5121
Size: 4898 223
DraIII CAC_nnn'GTG
Cuts at: 0 4255 4910 5121
Size: 4255 655 211
Fragments arranged by size:
4255 655 211
DrdI GACnn_nn'nnGTC
Cuts at: 0 3006 3760 4777 5121
Size: 3006 754 1017 344
Fragments arranged by size:
3006 1017 754 344
EaeI y'GGCC_r
Cuts at: 0 638 780 974 2519 3208 3767 3911 4716
Size: 638 142 194 1545 689 559 144 805
Cuts at: 4716 4817 4922 5121
Size: 101 105 199
Fragments arranged by size:
1545 805 689 638 559 199 194 144
142 105 101
EagI C'GGCC_G
Cuts at: 0 638 5121
Size: 638 4483
EarI CTCTTCn'nnn_
Cuts at: 0 356 427 663 1023 1059 1535 1737 2401
Size: 356 71 236 360 36 476 202 664
Cuts at: 2401 2831 3117 3168 3188 3194 3459 3719 3985
Size: 430 286 51 20 6 265 260 266
Cuts at: 3985 4079 4094 4106 5121
Size: 94 15 12 1015
Fragments arranged by size:
1015 664 476 430 360 356 286 266
265 260 236 202 94 71 51 36
20 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 1002 1802 4051 4199 4638 5121
Size: 66 936 800 2249 148 439 483
Fragments arranged by size:
2249 936 800 483 439 148 66
EcoICRI GAG'CTC
Cuts at: 0 1992 5121
Size: 1992 3129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 298 349 732 846 935 1199 1295
Size: 123 175 51 383 114 89 264 96
Cuts at: 1295 1507 1757 2584 2813 3206 3326 3530 3719
Size: 212 250 827 229 393 120 204 189
Cuts at: 3719 3913 3966 4027 4582 5121
Size: 194 53 61 555 539
Fragments arranged by size:
827 555 539 393 383 264 250 229
212 204 194 189 175 123 120 114
96 89 61 53 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1322 3718 5121
Size: 1322 2396 1403
Fragments arranged by size:
2396 1403 1322
EcoO109I rG'GnC_Cy
Cuts at: 0 33 594 1342 1610 2126 2657 2658 3358
Size: 33 561 748 268 516 531 1 700
Cuts at: 3358 3416 3869 4002 4011 4454 5121
Size: 58 453 133 9 443 667
Fragments arranged by size:
748 700 667 561 531 516 453 443
268 133 58 33 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1527 5121
Size: 1527 3594
FspI TGC'GCA
Cuts at: 0 97 5121
Size: 97 5024
HaeII r_GCGC'y
Cuts at: 0 443 1134 1212 1500 3864 4163 4200 4608
Size: 443 691 78 288 2364 299 37 408
Cuts at: 4608 4637 4839 5121
Size: 29 202 282
Fragments arranged by size:
2364 691 443 408 299 288 282 202
78 37 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1785 1817 2046 2078 2721 2753
Size: 754 32 999 32 229 32 643 32
Cuts at: 2753 2887 2919 3073 3105 3628 3658 3660 3690
Size: 134 32 154 32 523 30 2 30
Cuts at: 3690 4108 4140 4594 4626 5067 5099 5121
Size: 418 32 454 32 441 32 22
Fragments arranged by size:
999 754 643 523 454 441 418 229
154 134 32 32 32 32 32 32
32 32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 0 147 2060 5121
Size: 147 1913 3061
Fragments arranged by size:
3061 1913 147
HindIII A'AGCT_T
Cuts at: 0 741 5121
Size: 741 4380
HpaI GTT'AAC
Cuts at: 0 2060 5121
Size: 2060 3061
KasI G'GCGC_C
Cuts at: 0 439 4159 4196 5121
Size: 439 3720 37 925
Fragments arranged by size:
3720 925 439 37
KpnI G_GTAC'C
Cuts at: 0 4646 5121
Size: 4646 475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1781 2990 5121
Size: 1781 1209 2131
Fragments arranged by size:
2131 1781 1209
MscI TGG'CCA
Cuts at: 0 782 4924 5121
Size: 782 4142 197
Fragments arranged by size:
4142 782 197
MslI CAynn'nnrTG
Cuts at: 0 140 697 1477 1967 2257 3520 3902 5023
Size: 140 557 780 490 290 1263 382 1121
Cuts at: 5023 5121
Size: 98
Fragments arranged by size:
1263 1121 780 557 490 382 290 140
98
MspA1I CmG'CkG
Cuts at: 0 673 1204 2076 2110 3994 4071 4560 4620
Size: 673 531 872 34 1884 77 489 60
Cuts at: 4620 5121
Size: 501
Fragments arranged by size:
1884 872 673 531 501 489 77 60
34
NaeI GCC'GGC
Cuts at: 0 1255 5121
Size: 1255 3866
NarI GG'CG_CC
Cuts at: 0 440 4160 4197 5121
Size: 440 3720 37 924
Fragments arranged by size:
3720 924 440 37
NcoI C'CATG_G
Cuts at: 0 4751 5121
Size: 4751 370
NgoMIV G'CCGG_C
Cuts at: 0 1253 5121
Size: 1253 3868
NspI r_CATG'y
Cuts at: 0 4827 5121
Size: 4827 294
PasI CC'CwG_GG
Cuts at: 0 2318 2853 5121
Size: 2318 535 2268
Fragments arranged by size:
2318 2268 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 1318 3412 5121
Size: 1318 2094 1709
Fragments arranged by size:
2094 1709 1318
PfoI T'CCnGG_A
Cuts at: 0 2396 3890 4004 5121
Size: 2396 1494 114 1117
Fragments arranged by size:
2396 1494 1117 114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1349 1381 5121
Size: 1349 32 3740
Fragments arranged by size:
3740 1349 32
PpuMI rG'GwC_Cy
Cuts at: 0 4002 5121
Size: 4002 1119
PspOMI G'GGCC_C
Cuts at: 0 1342 1609 2657 3415 4453 5121
Size: 1342 267 1048 758 1038 668
Fragments arranged by size:
1342 1048 1038 758 668 267
PstI C_TGCA'G
Cuts at: 0 996 3357 4064 4355 5121
Size: 996 2361 707 291 766
Fragments arranged by size:
2361 996 766 707 291
PvuII CAG'CTG
Cuts at: 0 673 1204 2076 2110 3994 4560 5121
Size: 673 531 872 34 1884 566 561
Fragments arranged by size:
1884 872 673 566 561 531 34
RsrII CG'GwC_CG
Cuts at: 0 4610 5121
Size: 4610 511
SacI G_AGCT'C
Cuts at: 0 1994 5121
Size: 1994 3127
SapI GCTCTTCn'nnn_
Cuts at: 0 427 663 1737 2401 2831 3117 5121
Size: 427 236 1074 664 430 286 2004
Fragments arranged by size:
2004 1074 664 430 427 286 236
SbfI CC_TGCA'GG
Cuts at: 0 3357 5121
Size: 3357 1764
SexAI A'CCwGG_T
Cuts at: 0 1317 5121
Size: 1317 3804
SfcI C'TryA_G
Cuts at: 0 992 1658 3353 4060 4351 5121
Size: 992 666 1695 707 291 770
Fragments arranged by size:
1695 992 770 707 666 291
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3775 5121
Size: 3775 1346
SfoI GGC'GCC
Cuts at: 0 441 4161 4198 5121
Size: 441 3720 37 923
Fragments arranged by size:
3720 923 441 37
SmaI CCC'GGG
Cuts at: 0 1687 3601 4459 5121
Size: 1687 1914 858 662
Fragments arranged by size:
1914 1687 858 662
SmlI C'TyrA_G
Cuts at: 0 4687 5121
Size: 4687 434
SnaBI TAC'GTA
Cuts at: 0 4337 5121
Size: 4337 784
StuI AGG'CCT
Cuts at: 0 102 2140 2239 2733 3351 5121
Size: 102 2038 99 494 618 1770
Fragments arranged by size:
2038 1770 618 494 102 99
StyI C'CwwG_G
Cuts at: 0 80 443 612 804 1560 1697 1985 2534
Size: 80 363 169 192 756 137 288 549
Cuts at: 2534 3865 4751 4964 5121
Size: 1331 886 213 157
Fragments arranged by size:
1331 886 756 549 363 288 213 192
169 157 137 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1920 2015 2331 3495 5121
Size: 1920 95 316 1164 1626
Fragments arranged by size:
1920 1626 1164 316 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 1608 1640 2854 2886 5121
Size: 457 32 1119 32 1214 32 2235
Fragments arranged by size:
2235 1214 1119 457 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1685 3599 4457 5121
Size: 1685 1914 858 664
Fragments arranged by size:
1914 1685 858 664
XmnI GAAnn'nnTTC
Cuts at: 0 1364 5121
Size: 1364 3757
ZraI GAC'GTC
Cuts at: 0 2806 5121
Size: 2806 2315
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII RsrII SacI
SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI
SnaBI StuI StyI TaqII TstI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AloI ApaLI AscI
AseI AsiSI BaeI BclI BfrBI BmtI BsmI BspHI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I CspCI DraI
EcoRI EcoRV FseI FspAI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI SacII SalI SanDI ScaI
SgfI SgrAI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI