(Circular) (Six-base) MAPSORT of: pFN21AA0336.seq Check: 2478 from: 1 to: 9454 pFN21AA0336 9454 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7264 278 Size: 53 83 186 936 5728 2468 Fragments arranged by size: 5728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3411 4998 6797 1937 Size: 1474 1587 1799 4594 Fragments arranged by size: 4594 1799 1587 1474 Acc65I G'GTAC_C Cuts at: 3361 6775 3361 Size: 3414 6040 AclI AA'CG_TT Cuts at: 1563 5200 7581 7954 1563 Size: 3637 2381 373 3063 Fragments arranged by size: 3637 3063 2381 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 3753 3777 3878 4518 5009 5066 5099 Size: 618 1503 24 101 640 491 57 33 Cuts at: 5099 5313 6672 7517 8901 9169 1632 Size: 214 1359 845 1384 268 1917 Fragments arranged by size: 1917 1503 1384 1359 845 640 618 491 268 214 101 57 33 24 AflII C'TTAA_G Cuts at: 820 1017 2783 2987 3086 4187 4228 6125 820 Size: 197 1766 204 99 1101 41 1897 4149 Fragments arranged by size: 4149 1897 1766 1101 204 197 99 41 AflIII A'CryG_T Cuts at: 1203 4364 8353 9330 1203 Size: 3161 3989 977 1327 Fragments arranged by size: 3989 3161 1327 977 AgeI A'CCGG_T Cuts at: 8285 8285 Size: 9454 AhdI GACnn_n'nnGTC Cuts at: 5988 8183 5988 Size: 2195 7259 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9454 AlwNI CAG_nnn'CTG Cuts at: 5420 7197 8769 5420 Size: 1777 1572 6105 Fragments arranged by size: 6105 1777 1572 ApaLI G'TGCA_C Cuts at: 7511 8667 7511 Size: 1156 8298 ApoI r'AATT_y Cuts at: 1493 2098 2213 2292 2602 3165 3469 3514 4867 Size: 605 115 79 310 563 304 45 1353 Cuts at: 4867 4894 5274 5657 6365 6763 6989 7141 9250 Size: 27 380 383 708 398 226 152 2109 Cuts at: 9250 1493 Size: 1697 Fragments arranged by size: 2109 1697 1353 708 605 563 398 383 380 310 304 226 152 115 79 45 27 AseI AT'TA_AT Cuts at: 160 1032 2403 2862 3065 5823 8008 160 Size: 872 1371 459 203 2758 2185 1606 Fragments arranged by size: 2758 2185 1606 1371 872 459 203 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9454 AvaI C'yCGr_G Cuts at: 1870 1943 6234 6779 1870 Size: 73 4291 545 4545 Fragments arranged by size: 4545 4291 545 73 BamHI G'GATC_C Cuts at: 6784 7151 6784 Size: 367 9087 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 6775 8230 618 Size: 325 217 627 1574 3414 1455 1842 Fragments arranged by size: 3414 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6773 1391 Size: 5382 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2148 2850 3741 4023 4524 4851 5319 Size: 971 249 702 891 282 501 327 468 Cuts at: 5319 6273 928 Size: 954 4109 Fragments arranged by size: 4109 971 954 891 702 501 468 327 282 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7645 7679 1903 Size: 34 5708 34 3678 Fragments arranged by size: 5708 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7346 8556 7346 Size: 1210 8244 BclI T'GATC_A Cuts at: 1548 5422 1548 Size: 3874 5580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4628 8065 136 Size: 107 122 71 631 605 2956 3437 1525 Fragments arranged by size: 3437 2956 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4732 4909 9449 4732 Size: 177 4540 4737 Fragments arranged by size: 4737 4540 177 BlpI GC'TnA_GC Cuts at: 6644 6871 6644 Size: 227 9227 Bme1580I G_kGCm'C Cuts at: 1790 7515 8671 1790 Size: 5725 1156 2573 Fragments arranged by size: 5725 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3191 6146 7185 Size: 416 53 83 399 24 1774 2955 1039 Cuts at: 7185 8143 442 Size: 958 1753 Fragments arranged by size: 2955 1774 1753 1039 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2927 1056 Size: 1871 7583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3305 5480 8114 1239 Size: 2066 2175 2634 2579 Fragments arranged by size: 2634 2579 2175 2066 Bpu10I CC'TnA_GC Cuts at: 2490 4495 2490 Size: 2005 7449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6362 6666 7579 8444 8742 6362 Size: 304 913 865 298 7074 Fragments arranged by size: 7074 913 865 304 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4655 8117 882 Size: 617 3156 3462 2219 Fragments arranged by size: 3462 3156 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7245 493 Size: 711 6041 2702 Fragments arranged by size: 6041 2702 711 BsaBI GATnn'nnATC Cuts at: 6721 9448 6721 Size: 2727 6727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7261 7643 8278 275 Size: 53 83 186 936 5728 382 635 1451 Fragments arranged by size: 5728 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7887 8285 8559 8706 9117 7887 Size: 398 274 147 411 8224 Fragments arranged by size: 8224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6263 6293 1178 Size: 30 165 30 4860 30 4339 Fragments arranged by size: 4860 4339 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 3674 4607 1186 Size: 1114 1374 933 6033 Fragments arranged by size: 6033 1374 1114 933 BseYI C'CCAG_C Cuts at: 8657 9153 8657 Size: 496 8958 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 6435 1163 Size: 573 4699 4182 Fragments arranged by size: 4699 4182 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6904 7665 8693 1540 Size: 275 186 4903 761 1028 2301 Fragments arranged by size: 4903 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5541 7515 7600 8671 5541 Size: 1974 85 1071 6324 Fragments arranged by size: 6324 1974 1071 85 BsiWI C'GTAC_G Cuts at: 5570 5570 Size: 9454 BsmI GAATG_Cn' Cuts at: 4303 4583 6965 7058 4303 Size: 280 2382 93 6699 Fragments arranged by size: 6699 2382 280 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5541 6773 7515 7600 8671 1391 Size: 399 3751 1232 742 85 1071 2174 Fragments arranged by size: 3751 2174 1232 1071 742 399 85 BspEI T'CCGG_A Cuts at: 9117 9117 Size: 9454 BspHI T'CATG_A Cuts at: 2833 3250 7343 9073 2833 Size: 417 4093 1730 3214 Fragments arranged by size: 4093 3214 1730 417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 6809 844 Size: 2074 3891 3489 Fragments arranged by size: 3891 3489 2074 BsrBI CCG'CTC Cuts at: 1394 1823 7341 1394 Size: 429 5518 3507 Fragments arranged by size: 5518 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 4998 5843 7949 8123 65 Size: 1183 936 2814 845 2106 174 1396 Fragments arranged by size: 2814 2106 1396 1183 936 845 174 BsrFI r'CCGG_y Cuts at: 1700 1810 8098 8285 1700 Size: 110 6288 187 2869 Fragments arranged by size: 6288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9454 BssSI C'ACGA_G Cuts at: 5534 7514 8526 5534 Size: 1980 1012 6462 Fragments arranged by size: 6462 1980 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6458 794 Size: 5664 3790 BstBI TT'CG_AA Cuts at: 3216 6019 8258 3216 Size: 2803 2239 4412 Fragments arranged by size: 4412 2803 2239 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9454 BstXI CCAn_nnnn'nTGG Cuts at: 4993 4993 Size: 9454 BstYI r'GATC_y Cuts at: 1972 4732 4909 6784 7151 7536 7553 9005 9091 Size: 2760 177 1875 367 385 17 1452 86 Cuts at: 9091 9103 9449 1972 Size: 12 346 1977 Fragments arranged by size: 2760 1977 1875 1452 385 367 346 177 86 17 12 BstZ17I GTA'TAC Cuts at: 3412 3412 Size: 9454 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8902 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8715 Fragments arranged by size: 8715 683 56 BtsI GCAGTG_nn' Cuts at: 4886 5864 6970 7764 7784 4886 Size: 978 1106 794 20 6556 Fragments arranged by size: 6556 1106 978 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9454 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9419 DraI TTT'AAA Cuts at: 2857 2934 3135 3669 3924 5448 6758 2857 Size: 77 201 534 255 1524 1310 5553 Fragments arranged by size: 5553 1524 1310 534 255 201 77 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3034 4936 8461 809 Size: 507 1718 1902 3525 1802 Fragments arranged by size: 3525 1902 1802 1718 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6901 7790 9419 Size: 54 1425 50 275 18 5071 889 1629 Cuts at: 9419 8 Size: 43 Fragments arranged by size: 5071 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6901 1537 Size: 275 5089 4090 Fragments arranged by size: 5089 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2412 2445 3903 4020 4155 4698 5607 Size: 897 162 33 1458 117 135 543 909 Cuts at: 5607 6122 6234 7384 1353 Size: 515 112 1150 3423 Fragments arranged by size: 3423 1458 1150 909 897 543 515 162 135 117 112 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5930 6274 8041 8415 8561 387 Size: 1064 138 4341 344 1767 374 146 1280 Fragments arranged by size: 4341 1767 1280 1064 374 344 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 3305 3753 3777 3878 4518 5009 Size: 393 618 1055 448 24 101 640 491 Cuts at: 5009 5066 5099 5313 5480 6672 7517 8114 8901 Size: 57 33 214 167 1192 845 597 787 Cuts at: 8901 9169 1239 Size: 268 1524 Fragments arranged by size: 1524 1192 1055 845 787 640 618 597 491 448 393 268 214 167 101 57 33 24 EcoO109I rG'GnC_Cy Cuts at: 4267 6013 4267 Size: 1746 7708 EcoRI G'AATT_C Cuts at: 5657 6763 7141 5657 Size: 1106 378 7970 Fragments arranged by size: 7970 1106 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3314 4510 6583 3314 Size: 1196 2073 6185 Fragments arranged by size: 6185 2073 1196 FspI TGC'GCA Cuts at: 7960 7960 Size: 9454 HaeII r_GCGC'y Cuts at: 1678 5742 8601 1678 Size: 4064 2859 2531 Fragments arranged by size: 4064 2859 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3203 3235 3457 3489 4514 Size: 32 135 32 1631 32 222 32 1025 Cuts at: 4514 4546 4700 4732 5974 6006 6257 6263 6289 Size: 32 154 32 1242 32 251 6 26 Cuts at: 6289 6295 8095 8127 8169 8201 1373 Size: 6 1800 32 42 32 2626 Fragments arranged by size: 2626 1800 1631 1242 1025 251 222 154 135 42 32 32 32 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 5229 6798 7044 669 Size: 988 281 3291 1569 246 3079 Fragments arranged by size: 3291 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 6482 748 Size: 5734 3720 HpaI GTT'AAC Cuts at: 5229 7044 5229 Size: 1815 7639 KpnI G_GTAC'C Cuts at: 3365 6779 3365 Size: 3414 6040 MfeI C'AATT_G Cuts at: 5369 7053 5369 Size: 1684 7770 MluI A'CGCG_T Cuts at: 9330 9330 Size: 9454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 4527 8568 8752 1305 Size: 735 2487 4041 184 2007 Fragments arranged by size: 4041 2487 2007 735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7632 Fragments arranged by size: 7632 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5171 5960 7412 7771 7930 518 Size: 648 4005 789 1452 359 159 2042 Fragments arranged by size: 4005 2042 1452 789 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2167 3755 5500 6316 6870 7547 8695 8940 Size: 561 1588 1745 816 554 677 1148 245 Cuts at: 8940 1606 Size: 2120 Fragments arranged by size: 2120 1745 1588 1148 816 677 561 554 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8902 NdeI CA'TA_TG Cuts at: 387 387 Size: 9454 NheI G'CTAG_C Cuts at: 1052 2923 1052 Size: 1871 7583 NotI GC'GGCC_GC Cuts at: 6901 6901 Size: 9454 NspI r_CATG'y Cuts at: 1132 1229 4368 5054 6223 6362 6812 8357 1132 Size: 97 3139 686 1169 139 450 1545 2229 Fragments arranged by size: 3139 2229 1545 1169 686 450 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9454 PciI A'CATG_T Cuts at: 4364 8353 4364 Size: 3989 5465 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9454 PfoI T'CCnGG_A Cuts at: 5922 5922 Size: 9454 PmeI GTTT'AAAC Cuts at: 6758 6758 Size: 9454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7523 7555 9036 9068 7523 Size: 32 1481 32 7909 Fragments arranged by size: 7909 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 6013 6013 Size: 9454 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9454 PsiI TTA'TAA Cuts at: 3500 4409 6626 7024 3500 Size: 909 2217 398 5930 Fragments arranged by size: 5930 2217 909 398 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3265 3297 5070 5102 3265 Size: 32 1773 32 7617 Fragments arranged by size: 7617 1773 32 32 PstI C_TGCA'G Cuts at: 830 3261 4275 5895 6541 6576 6806 830 Size: 2431 1014 1620 646 35 230 3478 Fragments arranged by size: 3478 2431 1620 1014 646 230 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9454 PvuII CAG'CTG Cuts at: 2167 3755 5500 6316 2167 Size: 1588 1745 816 5305 Fragments arranged by size: 5305 1745 1588 816 SalI G'TCGA_C Cuts at: 1936 6796 1936 Size: 4860 4594 SapI GCTCTTCn'nnn_ Cuts at: 2412 3903 6122 2412 Size: 1491 2219 5744 Fragments arranged by size: 5744 2219 1491 SbfI CC_TGCA'GG Cuts at: 6806 6806 Size: 9454 ScaI AGT'ACT Cuts at: 7702 9414 7702 Size: 1712 7742 SexAI A'CCwGG_T Cuts at: 6592 6592 Size: 9454 SfcI C'TryA_G Cuts at: 826 1045 2955 3107 3257 4049 4271 5606 5891 Size: 219 1910 152 150 792 222 1335 285 Cuts at: 5891 6537 6572 6802 7937 8618 8809 826 Size: 646 35 230 1135 681 191 1471 Fragments arranged by size: 1910 1471 1335 1135 792 681 646 285 230 222 219 191 152 150 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9454 SmaI CCC'GGG Cuts at: 1872 6236 6781 1872 Size: 4364 545 4545 Fragments arranged by size: 4545 4364 545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 2987 3086 4187 4228 6125 Size: 197 926 840 204 99 1101 41 1897 Cuts at: 6125 6341 6681 7558 8459 8721 820 Size: 216 340 877 901 262 1553 Fragments arranged by size: 1897 1553 1101 926 901 877 840 340 262 216 204 197 99 41 SnaBI TAC'GTA Cuts at: 493 7245 493 Size: 6752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9454 SphI G_CATG'C Cuts at: 1132 6362 6812 1132 Size: 5230 450 3774 Fragments arranged by size: 5230 3774 450 SspI AAT'ATT Cuts at: 5 52 2401 7378 5 Size: 47 2349 4977 2081 Fragments arranged by size: 4977 2349 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9454 StyI C'CwwG_G Cuts at: 513 1065 4068 5628 6893 513 Size: 552 3003 1560 1265 3074 Fragments arranged by size: 3074 3003 1560 1265 552 SwaI ATTT'AAAT Cuts at: 3924 3924 Size: 9454 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7481 7498 7651 7836 8281 325 Size: 1255 5901 17 153 185 445 1498 Fragments arranged by size: 5901 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2977 3609 4960 Size: 274 80 33 51 1443 1000 632 1351 Cuts at: 4960 5332 7700 9412 96 Size: 372 2368 1712 138 Fragments arranged by size: 2368 1712 1443 1351 1000 632 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5918 5950 6102 6134 9414 9446 5918 Size: 32 152 32 3280 32 5926 Fragments arranged by size: 5926 3280 152 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9454 XbaI T'CTAG_A Cuts at: 4285 6214 6790 4285 Size: 1929 576 6949 Fragments arranged by size: 6949 1929 576 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5392 6379 5392 Size: 987 8467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9454 XmaI C'CCGG_G Cuts at: 1870 6234 6779 1870 Size: 4364 545 4545 Fragments arranged by size: 4545 4364 545 XmnI GAAnn'nnTTC Cuts at: 4692 5022 5079 6384 7583 4692 Size: 330 57 1305 1199 6563 Fragments arranged by size: 6563 1305 1199 330 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7262 276 Size: 53 83 186 936 5728 2468 Fragments arranged by size: 5728 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI