(Circular) (Six-base) MAPSORT of: pFN21AA0329.seq Check: 9527 from: 1 to: 8938 pFN21AA0329 8938 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4552 5853 4552 Size: 1301 7637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4256 6748 278 Size: 53 83 186 936 2720 2492 2468 Fragments arranged by size: 2720 2492 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6281 1937 Size: 4344 4594 Acc65I G'GTAC_C Cuts at: 6259 6259 Size: 8938 AclI AA'CG_TT Cuts at: 1563 3641 7065 7438 1563 Size: 2078 3424 373 3063 Fragments arranged by size: 3424 3063 2078 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2619 3090 5210 7001 8385 8653 1632 Size: 987 471 2120 1791 1384 268 1917 Fragments arranged by size: 2120 1917 1791 1384 987 471 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8741 AflIII A'CryG_T Cuts at: 1203 4150 7837 8814 1203 Size: 2947 3687 977 1327 Fragments arranged by size: 3687 2947 1327 977 AgeI A'CCGG_T Cuts at: 7769 7769 Size: 8938 AhdI GACnn_n'nnGTC Cuts at: 3517 7667 3517 Size: 4150 4788 AleI CACnn'nnGTG Cuts at: 1166 2246 1166 Size: 1080 7858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2186 4529 1184 Size: 1002 2343 5593 Fragments arranged by size: 5593 2343 1002 AlwNI CAG_nnn'CTG Cuts at: 2024 2297 3170 3322 3994 4351 4899 5360 5845 Size: 273 873 152 672 357 548 461 485 Cuts at: 5845 6089 6681 8253 2024 Size: 244 592 1572 2709 Fragments arranged by size: 2709 1572 873 672 592 548 485 461 357 273 244 152 ApaI G_GGCC'C Cuts at: 3976 4870 4999 5983 6119 3976 Size: 894 129 984 136 6795 Fragments arranged by size: 6795 984 894 136 129 ApaLI G'TGCA_C Cuts at: 3004 5873 6995 8151 3004 Size: 2869 1122 1156 3791 Fragments arranged by size: 3791 2869 1156 1122 ApoI r'AATT_y Cuts at: 1493 2651 3054 3476 5291 6057 6247 6473 6625 Size: 1158 403 422 1815 766 190 226 152 Cuts at: 6625 8734 1493 Size: 2109 1697 Fragments arranged by size: 2109 1815 1697 1158 766 422 403 226 190 152 AseI AT'TA_AT Cuts at: 160 1032 7492 160 Size: 872 6460 1606 Fragments arranged by size: 6460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8938 AvaI C'yCGr_G Cuts at: 1870 1943 2717 4423 4973 5246 5278 6263 1870 Size: 73 774 1706 550 273 32 985 4545 Fragments arranged by size: 4545 1706 985 774 550 273 73 32 BamHI G'GATC_C Cuts at: 2978 6268 6635 2978 Size: 3290 367 5281 Fragments arranged by size: 5281 3290 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2190 2865 4476 4520 5321 Size: 325 217 627 403 675 1611 44 801 Cuts at: 5321 6170 6259 7714 618 Size: 849 89 1455 1842 Fragments arranged by size: 1842 1611 1455 849 801 675 627 403 325 217 89 44 BanII G_rGCy'C Cuts at: 1391 2414 2490 2724 3270 3285 3976 4035 4326 Size: 1023 76 234 546 15 691 59 291 Cuts at: 4326 4588 4870 4999 5983 6119 6257 1391 Size: 262 282 129 984 136 138 4072 Fragments arranged by size: 4072 1023 984 691 546 291 282 262 234 138 136 129 76 59 15 BbeI G_GCGC'C Cuts at: 4524 4524 Size: 8938 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 3422 3894 928 Size: 971 335 1188 472 5972 Fragments arranged by size: 5972 1188 971 472 335 BbvCI CC'TCA_GC Cuts at: 3687 3965 4082 4445 3687 Size: 278 117 363 8180 Fragments arranged by size: 8180 363 278 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2146 2180 4954 4988 7129 7163 1903 Size: 34 209 34 2774 34 2141 34 3678 Fragments arranged by size: 3678 2774 2141 209 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6830 8040 6830 Size: 1210 7728 BclI T'GATC_A Cuts at: 1548 1548 Size: 8938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3280 5607 6123 Size: 107 122 71 631 605 1608 2327 516 Cuts at: 6123 7549 136 Size: 1426 1525 Fragments arranged by size: 2327 1608 1525 1426 631 605 516 122 107 71 BglII A'GATC_T Cuts at: 8933 8933 Size: 8938 BlpI GC'TnA_GC Cuts at: 2258 5960 6355 2258 Size: 3702 395 4841 Fragments arranged by size: 4841 3702 395 Bme1580I G_kGCm'C Cuts at: 1790 3008 3976 4870 4999 5877 5983 6119 6999 Size: 1218 968 894 129 878 106 136 880 Cuts at: 6999 8155 1790 Size: 1156 2573 Fragments arranged by size: 2573 1218 1156 968 894 880 878 136 129 106 BmgBI CAC'GTC Cuts at: 1318 5299 1318 Size: 3981 4957 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2655 3490 5742 Size: 416 53 83 399 24 1238 835 2252 Cuts at: 5742 6669 7627 442 Size: 927 958 1753 Fragments arranged by size: 2252 1753 1238 958 927 835 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4607 Size: 8938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2457 3122 3269 3558 3674 6236 7598 1239 Size: 1218 665 147 289 116 2562 1362 2579 Fragments arranged by size: 2579 2562 1362 1218 665 289 147 116 Bpu10I CC'TnA_GC Cuts at: 3297 3687 3965 4082 4352 4445 4700 5304 3297 Size: 390 278 117 270 93 255 604 6931 Fragments arranged by size: 6931 604 390 278 270 255 117 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3514 4244 4370 7063 7928 8226 3514 Size: 730 126 2693 865 298 4226 Fragments arranged by size: 4226 2693 865 730 298 126 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2569 4192 4462 5991 7601 882 Size: 617 1070 1623 270 1529 1610 2219 Fragments arranged by size: 2219 1623 1610 1529 1070 617 270 BsaAI yAC'GTr Cuts at: 493 1204 3133 5878 6729 493 Size: 711 1929 2745 851 2702 Fragments arranged by size: 2745 2702 1929 851 711 BsaBI GATnn'nnATC Cuts at: 3079 8932 3079 Size: 5853 3085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4253 4374 4521 6018 Size: 53 83 186 936 2720 121 147 1497 Cuts at: 6018 6745 7127 7762 275 Size: 727 382 635 1451 Fragments arranged by size: 2720 1497 1451 936 727 635 382 186 147 121 83 53 BsaWI w'CCGG_w Cuts at: 2981 4183 4903 7371 7769 8043 8190 8601 2981 Size: 1202 720 2468 398 274 147 411 3318 Fragments arranged by size: 3318 2468 1202 720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3245 3275 3427 3457 6209 Size: 30 165 30 1842 30 152 30 2752 Cuts at: 6209 6239 1178 Size: 30 3877 Fragments arranged by size: 3877 2752 1842 165 152 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2528 4280 5266 5912 6122 1186 Size: 865 477 1752 986 646 210 4002 Fragments arranged by size: 4002 1752 986 865 646 477 210 BseYI C'CCAG_C Cuts at: 2499 2901 3301 3969 4034 4341 4360 5217 5637 Size: 402 400 668 65 307 19 857 420 Cuts at: 5637 8141 8637 2499 Size: 2504 496 2800 Fragments arranged by size: 2800 2504 857 668 496 420 402 400 307 65 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 2798 3155 3585 4386 5134 5470 Size: 573 816 246 357 430 801 748 336 Cuts at: 5470 5513 1163 Size: 43 4588 Fragments arranged by size: 4588 816 801 748 573 430 357 336 246 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6388 7149 8177 1540 Size: 275 186 4387 761 1028 2301 Fragments arranged by size: 4387 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3008 3440 4326 5877 5966 6999 7084 8155 3008 Size: 432 886 1551 89 1033 85 1071 3791 Fragments arranged by size: 3791 1551 1071 1033 886 432 89 85 BsmI GAATG_Cn' Cuts at: 6449 6542 6449 Size: 93 8845 BsmBI CGTCTCn'nnnn_ Cuts at: 3167 4301 3167 Size: 1134 7804 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2414 2490 2724 3008 3270 3285 3440 Size: 399 624 76 234 284 262 15 155 Cuts at: 3440 3976 4035 4326 4588 4870 4999 5877 5966 Size: 536 59 291 262 282 129 878 89 Cuts at: 5966 5983 6119 6257 6999 7084 8155 1391 Size: 17 136 138 742 85 1071 2174 Fragments arranged by size: 2174 1071 878 742 624 536 399 291 284 282 262 262 234 155 138 136 129 89 85 76 59 17 15 BspEI T'CCGG_A Cuts at: 4903 8601 4903 Size: 3698 5240 BspHI T'CATG_A Cuts at: 6827 8557 6827 Size: 1730 7208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4552 5853 6293 844 Size: 3708 1301 440 3489 Fragments arranged by size: 3708 3489 1301 440 BsrBI CCG'CTC Cuts at: 1394 1823 2100 6825 1394 Size: 429 277 4725 3507 Fragments arranged by size: 4725 3507 429 277 BsrDI GCAATG_nn' Cuts at: 65 1248 2330 7433 7607 65 Size: 1183 1082 5103 174 1396 Fragments arranged by size: 5103 1396 1183 1082 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 4356 7582 7769 1700 Size: 110 377 2169 3226 187 2869 Fragments arranged by size: 3226 2869 2169 377 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8938 BssHII G'CGCG_C Cuts at: 1925 5518 1925 Size: 3593 5345 BssSI C'ACGA_G Cuts at: 6998 8010 6998 Size: 1012 7926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8938 BstBI TT'CG_AA Cuts at: 7742 7742 Size: 8938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8938 BstXI CCAn_nnnn'nTGG Cuts at: 3208 3734 3902 3208 Size: 526 168 8244 Fragments arranged by size: 8244 526 168 BstYI r'GATC_y Cuts at: 1972 2082 2473 2978 5271 6268 6635 7020 7037 Size: 110 391 505 2293 997 367 385 17 Cuts at: 7037 8489 8575 8587 8933 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2293 1977 1452 997 505 391 385 367 346 110 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4579 6207 4579 Size: 1628 7310 BtgI C'CryG_G Cuts at: 513 1065 2004 2746 3195 3207 4026 4235 4282 Size: 552 939 742 449 12 819 209 47 Cuts at: 4282 5008 5347 5487 5604 513 Size: 726 339 140 117 3847 Fragments arranged by size: 3847 939 819 742 726 552 449 339 209 140 117 47 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2131 4275 4719 487 Size: 683 56 905 2144 444 4706 Fragments arranged by size: 4706 2144 905 683 444 56 BtsI GCAGTG_nn' Cuts at: 3784 4692 4922 6454 7248 7268 3784 Size: 908 230 1532 794 20 5454 Fragments arranged by size: 5454 1532 908 794 230 20 ClaI AT'CG_AT Cuts at: 1554 2012 1554 Size: 458 8480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8903 DraI TTT'AAA Cuts at: 6242 6242 Size: 8938 DraIII CAC_nnn'GTG Cuts at: 5152 5152 Size: 8938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4252 7945 809 Size: 507 2936 3693 1802 Fragments arranged by size: 3693 2936 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3198 3210 4075 Size: 54 1425 50 275 18 1368 12 865 Cuts at: 4075 5154 5566 6385 7274 8903 8 Size: 1079 412 819 889 1629 43 Fragments arranged by size: 1629 1425 1368 1079 889 865 819 412 275 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 6385 1537 Size: 275 4573 4090 Fragments arranged by size: 4573 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3196 6173 6868 1353 Size: 1843 2977 695 3423 Fragments arranged by size: 3423 2977 1843 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7525 7899 8045 387 Size: 1064 138 5936 374 146 1280 Fragments arranged by size: 5936 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4324 4324 Size: 8938 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2457 2619 3090 3122 3269 3558 3674 Size: 393 825 162 471 32 147 289 116 Cuts at: 3674 5210 6236 7001 7598 8385 8653 1239 Size: 1536 1026 765 597 787 268 1524 Fragments arranged by size: 1536 1524 1026 825 787 765 597 471 393 289 268 162 147 116 32 EcoNI CCTnn'n_nnAGG Cuts at: 2299 4679 5567 2299 Size: 2380 888 5670 Fragments arranged by size: 5670 2380 888 EcoO109I rG'GnC_Cy Cuts at: 3973 4348 4427 4738 4866 4867 4996 5443 6211 Size: 375 79 311 128 1 129 447 768 Cuts at: 6211 3973 Size: 6700 Fragments arranged by size: 6700 768 447 375 311 129 128 79 1 EcoRI G'AATT_C Cuts at: 5291 6247 6625 5291 Size: 956 378 7604 Fragments arranged by size: 7604 956 378 EcoRV GAT'ATC Cuts at: 2016 2016 Size: 8938 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 4965 2233 Size: 2732 6206 FspI TGC'GCA Cuts at: 5201 7444 5201 Size: 2243 6695 HaeII r_GCGC'y Cuts at: 1678 4524 4853 5463 5555 5960 8085 1678 Size: 2846 329 610 92 405 2125 2531 Fragments arranged by size: 2846 2531 2125 610 405 329 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2352 2384 2504 2536 2978 Size: 32 135 32 780 32 120 32 442 Cuts at: 2978 3010 3082 3114 3245 3277 3427 3459 3503 Size: 32 72 32 131 32 150 32 44 Cuts at: 3503 3535 4753 4785 5722 5754 7579 7611 7653 Size: 32 1218 32 937 32 1825 32 42 Cuts at: 7653 7685 1373 Size: 32 2626 Fragments arranged by size: 2626 1825 1218 937 780 442 150 135 131 120 72 44 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2950 6282 6528 669 Size: 988 281 1012 3332 246 3079 Fragments arranged by size: 3332 3079 1012 988 281 246 HindIII A'AGCT_T Cuts at: 748 4947 5413 5662 748 Size: 4199 466 249 4024 Fragments arranged by size: 4199 4024 466 249 HpaI GTT'AAC Cuts at: 6528 6528 Size: 8938 KasI G'GCGC_C Cuts at: 4520 4520 Size: 8938 KpnI G_GTAC'C Cuts at: 6263 6263 Size: 8938 MfeI C'AATT_G Cuts at: 2961 6537 2961 Size: 3576 5362 MluI A'CGCG_T Cuts at: 8814 8814 Size: 8938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2099 2490 2894 3919 6160 8052 8236 1305 Size: 794 391 404 1025 2241 1892 184 2007 Fragments arranged by size: 2241 2007 1892 1025 794 404 391 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3200 3212 4077 5156 10 Size: 54 1425 343 1368 12 865 1079 3792 Fragments arranged by size: 3792 1425 1368 1079 865 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2168 2246 3732 5609 6896 7255 Size: 648 984 18 78 1486 1877 1287 359 Cuts at: 7255 7414 518 Size: 159 2042 Fragments arranged by size: 2042 1877 1486 1287 984 648 359 159 78 18 MspA1I CmG'CkG Cuts at: 1606 2503 2536 3258 3969 4177 4813 5262 5497 Size: 897 33 722 711 208 636 449 235 Cuts at: 5497 5953 5985 6354 7031 8179 8424 1606 Size: 456 32 369 677 1148 245 2120 Fragments arranged by size: 2120 1148 897 722 711 677 636 456 449 369 245 235 208 33 32 NaeI GCC'GGC Cuts at: 2189 4358 2189 Size: 2169 6769 NarI GG'CG_CC Cuts at: 4521 4521 Size: 8938 NcoI C'CATG_G Cuts at: 513 1065 2004 3195 5008 513 Size: 552 939 1191 1813 4443 Fragments arranged by size: 4443 1813 1191 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8938 NgoMIV G'CCGG_C Cuts at: 2187 4356 2187 Size: 2169 6769 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8938 NotI GC'GGCC_GC Cuts at: 6385 6385 Size: 8938 NspI r_CATG'y Cuts at: 1132 1229 2173 4103 5614 5673 5930 6296 7841 Size: 97 944 1930 1511 59 257 366 1545 Cuts at: 7841 1132 Size: 2229 Fragments arranged by size: 2229 1930 1545 1511 944 366 257 97 59 PasI CC'CwG_GG Cuts at: 1348 3278 1348 Size: 1930 7008 PciI A'CATG_T Cuts at: 7837 7837 Size: 8938 PflMI CCAn_nnn'nTGG Cuts at: 1108 4564 1108 Size: 3456 5482 PfoI T'CCnGG_A Cuts at: 3651 3651 Size: 8938 PmeI GTTT'AAAC Cuts at: 6242 6242 Size: 8938 PmlI CAC'GTG Cuts at: 3133 5878 3133 Size: 2745 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7007 7039 8520 8552 7007 Size: 32 1481 32 7393 Fragments arranged by size: 7393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4348 4427 4738 5443 6211 4348 Size: 79 311 705 768 7075 Fragments arranged by size: 7075 768 705 311 79 PshAI GACnn'nnGTC Cuts at: 1613 4122 4184 1613 Size: 2509 62 6367 Fragments arranged by size: 6367 2509 62 PsiI TTA'TAA Cuts at: 3050 6508 3050 Size: 3458 5480 PspOMI G'GGCC_C Cuts at: 3972 4866 4995 5979 6115 3972 Size: 894 129 984 136 6795 Fragments arranged by size: 6795 984 894 136 129 PstI C_TGCA'G Cuts at: 830 3817 4508 5395 6290 830 Size: 2987 691 887 895 3478 Fragments arranged by size: 3478 2987 895 887 691 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8938 PvuII CAG'CTG Cuts at: 2503 2536 3969 4177 5497 5953 2503 Size: 33 1433 208 1320 456 5488 Fragments arranged by size: 5488 1433 1320 456 208 33 RsrII CG'GwC_CG Cuts at: 5883 5883 Size: 8938 SacI G_AGCT'C Cuts at: 4326 4326 Size: 8938 SalI G'TCGA_C Cuts at: 1936 6280 1936 Size: 4344 4594 SanDI GG'GwC_CC Cuts at: 4427 4427 Size: 8938 SapI GCTCTTCn'nnn_ Cuts at: 3196 6173 3196 Size: 2977 5961 SbfI CC_TGCA'GG Cuts at: 6290 6290 Size: 8938 ScaI AGT'ACT Cuts at: 2644 7186 8898 2644 Size: 4542 1712 2684 Fragments arranged by size: 4542 2684 1712 SexAI A'CCwGG_T Cuts at: 2274 2274 Size: 8938 SfcI C'TryA_G Cuts at: 826 1045 3813 4504 5391 6286 7421 8102 8293 Size: 219 2768 691 887 895 1135 681 191 Cuts at: 8293 826 Size: 1471 Fragments arranged by size: 2768 1471 1135 895 887 691 681 219 191 SfoI GGC'GCC Cuts at: 4522 4522 Size: 8938 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8938 SmaI CCC'GGG Cuts at: 1872 2719 6265 1872 Size: 847 3546 4545 Fragments arranged by size: 4545 3546 847 SmlI C'TyrA_G Cuts at: 820 1017 1943 3529 4223 4385 7042 7943 8205 Size: 197 926 1586 694 162 2657 901 262 Cuts at: 8205 820 Size: 1553 Fragments arranged by size: 2657 1586 1553 926 901 694 262 197 162 SnaBI TAC'GTA Cuts at: 493 6729 493 Size: 6236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8938 SphI G_CATG'C Cuts at: 1132 2173 4103 5614 6296 1132 Size: 1041 1930 1511 682 3774 Fragments arranged by size: 3774 1930 1511 1041 682 SrfI GCCC'GGGC Cuts at: 2719 2719 Size: 8938 SspI AAT'ATT Cuts at: 5 52 3021 6862 5 Size: 47 2969 3841 2081 Fragments arranged by size: 3841 2969 2081 47 StuI AGG'CCT Cuts at: 1517 3067 4070 5945 1517 Size: 1550 1003 1875 4510 Fragments arranged by size: 4510 1875 1550 1003 StyI C'CwwG_G Cuts at: 513 1065 2004 2633 3195 3221 4194 5008 5053 Size: 552 939 629 562 26 973 814 45 Cuts at: 5053 6377 513 Size: 1324 3074 Fragments arranged by size: 3074 1324 973 939 814 629 562 552 45 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 4352 5839 6188 6965 6982 7135 Size: 1255 1313 1459 1487 349 777 17 153 Cuts at: 7135 7320 7765 325 Size: 185 445 1498 Fragments arranged by size: 1498 1487 1459 1313 1255 777 445 349 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2181 2211 Size: 274 80 33 51 1443 75 129 30 Cuts at: 2211 2642 7184 8896 96 Size: 431 4542 1712 138 Fragments arranged by size: 4542 1712 1443 431 274 138 129 80 75 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2641 2673 5531 5563 6209 6241 8898 8930 2641 Size: 32 2858 32 646 32 2657 32 2649 Fragments arranged by size: 2858 2657 2649 646 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2945 4931 6091 1316 Size: 1629 1986 1160 4163 Fragments arranged by size: 4163 1986 1629 1160 XbaI T'CTAG_A Cuts at: 2476 6274 2476 Size: 3798 5140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 3298 5149 2533 Size: 765 1851 6322 Fragments arranged by size: 6322 1851 765 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8938 XmaI C'CCGG_G Cuts at: 1870 2717 6263 1870 Size: 847 3546 4545 Fragments arranged by size: 4545 3546 847 XmnI GAAnn'nnTTC Cuts at: 2093 2429 5291 7067 2093 Size: 336 2862 1776 3964 Fragments arranged by size: 3964 2862 1776 336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4254 6746 276 Size: 53 83 186 936 2720 2492 2468 Fragments arranged by size: 2720 2492 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PspXI PsrI SacII SfiI SwaI