(Circular) (Six-base) MAPSORT of: pFN21AA0327.seq Check: 1522 from: 1 to: 7165 pFN21AA0327 7165 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4975 278 Size: 53 83 186 936 3439 2468 Fragments arranged by size: 3439 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4050 4508 1937 Size: 2113 458 4594 Fragments arranged by size: 4594 2113 458 Acc65I G'GTAC_C Cuts at: 4486 4486 Size: 7165 AclI AA'CG_TT Cuts at: 1563 5292 5665 1563 Size: 3729 373 3063 Fragments arranged by size: 3729 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 5228 6612 6880 1632 Size: 2426 1170 1384 268 1917 Fragments arranged by size: 2426 1917 1384 1170 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6968 AflIII A'CryG_T Cuts at: 1203 3161 3639 6064 7041 1203 Size: 1958 478 2425 977 1327 Fragments arranged by size: 2425 1958 1327 977 478 AgeI A'CCGG_T Cuts at: 3536 5996 3536 Size: 2460 4705 AhdI GACnn_n'nnGTC Cuts at: 5894 5894 Size: 7165 AleI CACnn'nnGTG Cuts at: 1166 3725 1166 Size: 2559 4606 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2244 2801 1184 Size: 1060 557 5548 Fragments arranged by size: 5548 1060 557 AlwNI CAG_nnn'CTG Cuts at: 2474 3595 4097 4908 6480 2474 Size: 1121 502 811 1572 3159 Fragments arranged by size: 3159 1572 1121 811 502 ApaLI G'TGCA_C Cuts at: 5222 6378 5222 Size: 1156 6009 ApoI r'AATT_y Cuts at: 1493 2399 2843 3079 4474 4700 4852 6961 1493 Size: 906 444 236 1395 226 152 2109 1697 Fragments arranged by size: 2109 1697 1395 906 444 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 2856 5719 160 Size: 872 1824 2863 1606 Fragments arranged by size: 2863 1824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 AvaI C'yCGr_G Cuts at: 1870 1943 3089 3683 3796 4490 1870 Size: 73 1146 594 113 694 4545 Fragments arranged by size: 4545 1146 694 594 113 73 BamHI G'GATC_C Cuts at: 4495 4862 4495 Size: 367 6798 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3499 3736 4486 5941 618 Size: 325 217 627 1712 237 750 1455 1842 Fragments arranged by size: 1842 1712 1455 750 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2289 2303 3898 4146 4484 1391 Size: 898 14 1595 248 338 4072 Fragments arranged by size: 4072 1595 898 338 248 14 BbeI G_GCGC'C Cuts at: 3503 3740 3503 Size: 237 6928 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4105 928 Size: 971 2206 3988 Fragments arranged by size: 3988 2206 971 BbvCI CC'TCA_GC Cuts at: 3629 4151 3629 Size: 522 6643 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5356 5390 1903 Size: 34 3419 34 3678 Fragments arranged by size: 3678 3419 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2698 5057 6267 2698 Size: 2359 1210 3596 Fragments arranged by size: 3596 2359 1210 BclI T'GATC_A Cuts at: 1548 2256 3045 4417 1548 Size: 708 789 1372 4296 Fragments arranged by size: 4296 1372 789 708 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5776 136 Size: 107 122 71 631 605 4104 1525 Fragments arranged by size: 4104 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2413 3280 7160 2413 Size: 867 3880 2418 Fragments arranged by size: 3880 2418 867 BlpI GC'TnA_GC Cuts at: 2519 4582 2519 Size: 2063 5102 Bme1580I G_kGCm'C Cuts at: 1790 2983 5226 6382 1790 Size: 1193 2243 1156 2573 Fragments arranged by size: 2573 2243 1193 1156 BmgBI CAC'GTC Cuts at: 1318 2548 1318 Size: 1230 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2907 3062 4896 Size: 416 53 83 399 24 1490 155 1834 Cuts at: 4896 5854 442 Size: 958 1753 Fragments arranged by size: 1834 1753 1490 958 416 399 155 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7165 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3932 Size: 7165 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2191 2469 2489 2577 2612 4163 5825 1239 Size: 952 278 20 88 35 1551 1662 2579 Fragments arranged by size: 2579 1662 1551 952 278 88 35 20 Bpu10I CC'TnA_GC Cuts at: 3629 4145 4151 3629 Size: 516 6 6643 Fragments arranged by size: 6643 516 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 3130 3815 3902 5290 6155 6453 2780 Size: 350 685 87 1388 865 298 3492 Fragments arranged by size: 3492 1388 865 685 350 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3576 5828 882 Size: 617 2077 2252 2219 Fragments arranged by size: 2252 2219 2077 617 BsaAI yAC'GTr Cuts at: 493 1204 2692 3164 3328 4956 493 Size: 711 1488 472 164 1628 2702 Fragments arranged by size: 2702 1628 1488 711 472 164 BsaBI GATnn'nnATC Cuts at: 7159 7159 Size: 7165 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3500 3737 4972 5354 Size: 53 83 186 936 1967 237 1235 382 Cuts at: 5354 5989 275 Size: 635 1451 Fragments arranged by size: 1967 1451 1235 936 635 382 237 186 83 53 BsaWI w'CCGG_w Cuts at: 2847 3536 4187 5598 5996 6270 6417 6828 2847 Size: 689 651 1411 398 274 147 411 3184 Fragments arranged by size: 3184 1411 689 651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2522 2552 4161 Size: 30 165 30 711 30 378 30 1609 Cuts at: 4161 4191 1178 Size: 30 4152 Fragments arranged by size: 4152 1609 711 378 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 3420 4250 1186 Size: 1111 1123 830 4101 Fragments arranged by size: 4101 1123 1111 830 BseYI C'CCAG_C Cuts at: 2062 4317 6368 6864 2062 Size: 2255 2051 496 2363 Fragments arranged by size: 2363 2255 2051 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 3308 3414 3851 1163 Size: 573 834 738 106 437 4477 Fragments arranged by size: 4477 834 738 573 437 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4050 4615 5376 6404 1540 Size: 275 186 2049 565 761 1028 2301 Fragments arranged by size: 2301 2049 1028 761 565 275 186 BsiHKAI G_wGCw'C Cuts at: 2289 2455 5226 5311 6382 2289 Size: 166 2771 85 1071 3072 Fragments arranged by size: 3072 2771 1071 166 85 BsmI GAATG_Cn' Cuts at: 3460 4676 4769 3460 Size: 1216 93 5856 Fragments arranged by size: 5856 1216 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2111 2209 3903 2111 Size: 98 1694 5373 Fragments arranged by size: 5373 1694 98 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2303 2455 2983 3898 4146 4484 Size: 399 499 14 152 528 915 248 338 Cuts at: 4484 5226 5311 6382 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 915 742 528 499 399 338 248 152 85 14 BspEI T'CCGG_A Cuts at: 2847 6828 2847 Size: 3981 3184 BspHI T'CATG_A Cuts at: 4396 5054 6784 4396 Size: 658 1730 4777 Fragments arranged by size: 4777 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3594 4520 844 Size: 2750 926 3489 Fragments arranged by size: 3489 2750 926 BsrBI CCG'CTC Cuts at: 1394 1823 2012 3744 5052 1394 Size: 429 189 1732 1308 3507 Fragments arranged by size: 3507 1732 1308 429 189 BsrDI GCAATG_nn' Cuts at: 65 1248 5660 5834 65 Size: 1183 4412 174 1396 Fragments arranged by size: 4412 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3536 5809 5996 1700 Size: 110 1726 2273 187 2869 Fragments arranged by size: 2869 2273 1726 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7165 BssHII G'CGCG_C Cuts at: 1925 2055 2596 1925 Size: 130 541 6494 Fragments arranged by size: 6494 541 130 BssSI C'ACGA_G Cuts at: 3929 4082 5225 6237 3929 Size: 153 1143 1012 4857 Fragments arranged by size: 4857 1143 1012 153 BstAPI GCAn_nnn'nTGC Cuts at: 794 4097 794 Size: 3303 3862 BstBI TT'CG_AA Cuts at: 5969 5969 Size: 7165 BstEII G'GTnAC_C Cuts at: 1184 3766 1184 Size: 2582 4583 BstXI CCAn_nnnn'nTGG Cuts at: 2158 3131 3727 3778 3950 2158 Size: 973 596 51 172 5373 Fragments arranged by size: 5373 973 596 172 51 BstYI r'GATC_y Cuts at: 1972 2413 3280 3576 3687 4495 4862 5247 5264 Size: 441 867 296 111 808 367 385 17 Cuts at: 5264 6716 6802 6814 7160 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 867 808 441 385 367 346 296 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 2303 3949 3993 513 Size: 552 939 258 41 1646 44 3685 Fragments arranged by size: 3685 1646 939 552 258 44 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6426 Fragments arranged by size: 6426 683 56 BtsI GCAGTG_nn' Cuts at: 4098 4681 5475 5495 4098 Size: 583 794 20 5768 Fragments arranged by size: 5768 794 583 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7165 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7130 DraI TTT'AAA Cuts at: 3181 4469 3181 Size: 1288 5877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3539 6172 809 Size: 507 2223 2633 1802 Fragments arranged by size: 2633 2223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3936 3952 Size: 54 1425 50 275 18 177 1929 16 Cuts at: 3952 4612 5501 7130 8 Size: 660 889 1629 43 Fragments arranged by size: 1929 1629 1425 889 660 275 177 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 4612 1537 Size: 275 2800 4090 Fragments arranged by size: 4090 2800 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2610 3090 3857 4292 5095 1353 Size: 756 501 480 767 435 803 3423 Fragments arranged by size: 3423 803 767 756 501 480 435 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3958 5752 6126 6272 387 Size: 1064 138 2369 1794 374 146 1280 Fragments arranged by size: 2369 1794 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2287 2287 Size: 7165 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2469 2489 2577 2612 4058 4163 Size: 393 559 278 20 88 35 1446 105 Cuts at: 4163 5228 5825 6612 6880 1239 Size: 1065 597 787 268 1524 Fragments arranged by size: 1524 1446 1065 787 597 559 393 278 268 105 88 35 20 EcoNI CCTnn'n_nnAGG Cuts at: 2333 3830 4178 2333 Size: 1497 348 5320 Fragments arranged by size: 5320 1497 348 EcoO109I rG'GnC_Cy Cuts at: 2155 3619 2155 Size: 1464 5701 EcoRI G'AATT_C Cuts at: 4474 4852 4474 Size: 378 6787 FspI TGC'GCA Cuts at: 2684 3884 5671 2684 Size: 1200 1787 4178 Fragments arranged by size: 4178 1787 1200 FspAI rTGC'GCAy Cuts at: 2684 3884 2684 Size: 1200 5965 HaeII r_GCGC'y Cuts at: 1678 3503 3740 4159 6312 1678 Size: 1825 237 419 2153 2531 Fragments arranged by size: 2531 2153 1825 419 237 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2184 2216 2739 Size: 32 135 32 542 32 38 32 523 Cuts at: 2739 2771 3113 3145 3231 3263 3399 3431 3779 Size: 32 342 32 86 32 136 32 348 Cuts at: 3779 3811 5806 5838 5880 5912 1373 Size: 32 1995 32 42 32 2626 Fragments arranged by size: 2626 1995 542 523 348 342 136 135 86 42 38 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 4051 4380 4509 4755 669 Size: 988 281 541 1572 329 129 246 3079 Fragments arranged by size: 3079 1572 988 541 329 281 246 129 HindIII A'AGCT_T Cuts at: 748 748 Size: 7165 HpaI GTT'AAC Cuts at: 4380 4755 4380 Size: 375 6790 KasI G'GCGC_C Cuts at: 3499 3736 3499 Size: 237 6928 KpnI G_GTAC'C Cuts at: 4490 4490 Size: 7165 MfeI C'AATT_G Cuts at: 4764 4764 Size: 7165 MluI A'CGCG_T Cuts at: 7041 7041 Size: 7165 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2682 6279 6463 1305 Size: 1377 3597 184 2007 Fragments arranged by size: 3597 2007 1377 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3954 10 Size: 54 1425 343 2122 3221 Fragments arranged by size: 3221 2122 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3011 3269 3725 5123 5482 5641 Size: 648 1524 321 258 456 1398 359 159 Cuts at: 5641 518 Size: 2042 Fragments arranged by size: 2042 1524 1398 648 456 359 321 258 159 MspA1I CmG'CkG Cuts at: 1606 2247 2264 2305 3615 4581 5258 6406 6651 Size: 641 17 41 1310 966 677 1148 245 Cuts at: 6651 1606 Size: 2120 Fragments arranged by size: 2120 1310 1148 966 677 641 245 41 17 NarI GG'CG_CC Cuts at: 3500 3737 3500 Size: 237 6928 NcoI C'CATG_G Cuts at: 513 1065 2004 3949 513 Size: 552 939 1945 3729 Fragments arranged by size: 3729 1945 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7165 NotI GC'GGCC_GC Cuts at: 4612 4612 Size: 7165 NspI r_CATG'y Cuts at: 1132 1229 2770 3643 4339 4523 6068 1132 Size: 97 1541 873 696 184 1545 2229 Fragments arranged by size: 2229 1545 1541 873 696 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7165 PciI A'CATG_T Cuts at: 3639 6064 3639 Size: 2425 4740 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7165 PfoI T'CCnGG_A Cuts at: 2446 3942 4179 2446 Size: 1496 237 5432 Fragments arranged by size: 5432 1496 237 PmeI GTTT'AAAC Cuts at: 4469 4469 Size: 7165 PmlI CAC'GTG Cuts at: 2692 3164 3328 2692 Size: 472 164 6529 Fragments arranged by size: 6529 472 164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3312 5234 5266 6747 6779 3280 Size: 32 1922 32 1481 32 3666 Fragments arranged by size: 3666 1922 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 3619 2155 Size: 1464 5701 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7165 PsiI TTA'TAA Cuts at: 4735 4735 Size: 7165 PstI C_TGCA'G Cuts at: 830 3495 3591 4257 4517 830 Size: 2665 96 666 260 3478 Fragments arranged by size: 3478 2665 666 260 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7165 SacI G_AGCT'C Cuts at: 2289 2289 Size: 7165 SacII CC_GC'GG Cuts at: 2265 2306 2265 Size: 41 7124 SalI G'TCGA_C Cuts at: 1936 4049 4507 1936 Size: 2113 458 4594 Fragments arranged by size: 4594 2113 458 SanDI GG'GwC_CC Cuts at: 3619 3619 Size: 7165 SbfI CC_TGCA'GG Cuts at: 4517 4517 Size: 7165 ScaI AGT'ACT Cuts at: 5413 7125 5413 Size: 1712 5453 SexAI A'CCwGG_T Cuts at: 2631 3762 2631 Size: 1131 6034 SfcI C'TryA_G Cuts at: 826 1045 3491 3587 4253 4513 5648 6329 6520 Size: 219 2446 96 666 260 1135 681 191 Cuts at: 6520 826 Size: 1471 Fragments arranged by size: 2446 1471 1135 681 666 260 219 191 96 SfoI GGC'GCC Cuts at: 3501 3738 3501 Size: 237 6928 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7165 SgrAI Cr'CCGG_yG Cuts at: 1700 3536 1700 Size: 1836 5329 SmaI CCC'GGG Cuts at: 1872 3091 4492 1872 Size: 1219 1401 4545 Fragments arranged by size: 4545 1401 1219 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 3109 3830 3917 5269 6170 Size: 197 926 816 350 721 87 1352 901 Cuts at: 6170 6432 820 Size: 262 1553 Fragments arranged by size: 1553 1352 926 901 816 721 350 262 197 87 SnaBI TAC'GTA Cuts at: 493 4956 493 Size: 4463 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7165 SphI G_CATG'C Cuts at: 1132 4523 1132 Size: 3391 3774 SspI AAT'ATT Cuts at: 5 52 2320 3230 5089 5 Size: 47 2268 910 1859 2081 Fragments arranged by size: 2268 2081 1859 910 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7165 StyI C'CwwG_G Cuts at: 513 1065 2004 2151 3771 3949 4604 513 Size: 552 939 147 1620 178 655 3074 Fragments arranged by size: 3074 1620 939 655 552 178 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3252 3494 4036 4037 4217 5192 5209 Size: 1255 1672 242 542 1 180 975 17 Cuts at: 5209 5362 5547 5992 325 Size: 153 185 445 1498 Fragments arranged by size: 1672 1498 1255 975 542 445 242 185 180 153 17 1 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5411 7123 96 Size: 274 80 33 51 1443 3434 1712 138 Fragments arranged by size: 3434 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2522 2554 4161 4193 7125 7157 2522 Size: 32 1607 32 2932 32 2530 Fragments arranged by size: 2932 2530 1607 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7165 XbaI T'CTAG_A Cuts at: 2416 2923 4501 2416 Size: 507 1578 5080 Fragments arranged by size: 5080 1578 507 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4187 4187 Size: 7165 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7165 XmaI C'CCGG_G Cuts at: 1870 3089 4490 1870 Size: 1219 1401 4545 Fragments arranged by size: 4545 1401 1219 XmnI GAAnn'nnTTC Cuts at: 5294 5294 Size: 7165 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4973 276 Size: 53 83 186 936 3439 2468 Fragments arranged by size: 3439 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SapI SfiI SrfI SwaI