(Circular) (Six-base) MAPSORT of: pFN21AA0311.seq Check: 3985 from: 1 to: 11662 pFN21AA0311 11662 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5407 9472 278 Size: 53 83 186 936 3871 4065 2468 Fragments arranged by size: 4065 3871 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2907 4491 4814 5566 9005 1937 Size: 970 1584 323 752 3439 4594 Fragments arranged by size: 4594 3439 1584 970 752 323 Acc65I G'GTAC_C Cuts at: 8983 8983 Size: 11662 AclI AA'CG_TT Cuts at: 1563 9789 10162 1563 Size: 8226 373 3063 Fragments arranged by size: 8226 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2586 2973 4595 7317 8802 9725 11109 11377 Size: 954 387 1622 2722 1485 923 1384 268 Cuts at: 11377 1632 Size: 1917 Fragments arranged by size: 2722 1917 1622 1485 1384 954 923 387 268 AflII C'TTAA_G Cuts at: 820 1017 3914 820 Size: 197 2897 8568 Fragments arranged by size: 8568 2897 197 AflIII A'CryG_T Cuts at: 1203 5561 6225 7567 10561 11538 1203 Size: 4358 664 1342 2994 977 1327 Fragments arranged by size: 4358 2994 1342 1327 977 664 AgeI A'CCGG_T Cuts at: 10493 10493 Size: 11662 AhdI GACnn_n'nnGTC Cuts at: 4939 10391 4939 Size: 5452 6210 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 11662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2385 8429 1184 Size: 1201 6044 4417 Fragments arranged by size: 6044 4417 1201 AlwNI CAG_nnn'CTG Cuts at: 2050 2189 2876 4037 4294 7184 8715 9405 10977 Size: 139 687 1161 257 2890 1531 690 1572 Cuts at: 10977 2050 Size: 2735 Fragments arranged by size: 2890 2735 1572 1531 1161 690 687 257 139 ApaI G_GGCC'C Cuts at: 4152 4152 Size: 11662 ApaLI G'TGCA_C Cuts at: 9719 10875 9719 Size: 1156 10506 ApoI r'AATT_y Cuts at: 1493 3419 3435 3643 4027 4373 6079 6239 7356 Size: 1926 16 208 384 346 1706 160 1117 Cuts at: 7356 7597 7856 8971 9197 9349 11458 1493 Size: 241 259 1115 226 152 2109 1697 Fragments arranged by size: 2109 1926 1706 1697 1117 1115 384 346 259 241 226 208 160 152 16 AseI AT'TA_AT Cuts at: 160 1032 5127 10216 160 Size: 872 4095 5089 1606 Fragments arranged by size: 5089 4095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11662 AvaI C'yCGr_G Cuts at: 1870 1943 8434 8987 1870 Size: 73 6491 553 4545 Fragments arranged by size: 6491 4545 553 73 AvrII C'CTAG_G Cuts at: 5158 5158 Size: 11662 BamHI G'GATC_C Cuts at: 2053 6490 8992 9359 2053 Size: 4437 2502 367 4356 Fragments arranged by size: 4437 4356 2502 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2457 3517 3787 8983 10438 Size: 325 217 627 670 1060 270 5196 1455 Cuts at: 10438 618 Size: 1842 Fragments arranged by size: 5196 1842 1455 1060 670 627 325 270 217 BanII G_rGCy'C Cuts at: 1391 3584 4152 4660 4917 6938 7095 8981 1391 Size: 2193 568 508 257 2021 157 1886 4072 Fragments arranged by size: 4072 2193 2021 1886 568 508 257 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2937 3324 7056 7371 928 Size: 971 1038 387 3732 315 5219 Fragments arranged by size: 5219 3732 1038 971 387 315 BbvCI CC'TCA_GC Cuts at: 4737 8670 4737 Size: 3933 7729 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2447 2481 5387 5421 8231 8265 9853 Size: 34 510 34 2906 34 2810 34 1588 Cuts at: 9853 9887 1903 Size: 34 3678 Fragments arranged by size: 3678 2906 2810 1588 510 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4657 9554 10764 4657 Size: 4897 1210 5555 Fragments arranged by size: 5555 4897 1210 BclI T'GATC_A Cuts at: 1548 2350 5499 5719 1548 Size: 802 3149 220 7491 Fragments arranged by size: 7491 3149 802 220 BfrBI ATG'CAT Cuts at: 7151 8956 7151 Size: 1805 9857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10273 136 Size: 107 122 71 631 605 8601 1525 Fragments arranged by size: 8601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6016 6826 6904 11657 6016 Size: 810 78 4753 6021 Fragments arranged by size: 6021 4753 810 78 BlpI GC'TnA_GC Cuts at: 3583 9079 3583 Size: 5496 6166 Bme1580I G_kGCm'C Cuts at: 1790 4152 5193 9723 10879 1790 Size: 2362 1041 4530 1156 2573 Fragments arranged by size: 4530 2573 2362 1156 1041 BmgBI CAC'GTC Cuts at: 1318 3844 5564 1318 Size: 2526 1720 7416 Fragments arranged by size: 7416 2526 1720 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2981 4018 9393 Size: 416 53 83 399 24 1564 1037 5375 Cuts at: 9393 10351 442 Size: 958 1753 Fragments arranged by size: 5375 1753 1564 1037 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11662 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8509 8509 Size: 11662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4481 5273 6705 6758 7258 7318 10322 1239 Size: 3242 792 1432 53 500 60 3004 2579 Fragments arranged by size: 3242 3004 2579 1432 792 500 60 53 Bpu10I CC'TnA_GC Cuts at: 2802 4737 8670 2802 Size: 1935 3933 5794 Fragments arranged by size: 5794 3933 1935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2874 3686 4492 4493 4565 4778 9787 10652 10950 Size: 812 806 1 72 213 5009 865 298 Cuts at: 10950 2874 Size: 3586 Fragments arranged by size: 5009 3586 865 812 806 298 213 72 1 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 2253 7535 10325 882 Size: 617 720 34 5282 2790 2219 Fragments arranged by size: 5282 2790 2219 720 617 34 BsaAI yAC'GTr Cuts at: 493 1204 5210 9453 493 Size: 711 4006 4243 2702 Fragments arranged by size: 4243 4006 2702 711 BsaBI GATnn'nnATC Cuts at: 2310 11656 2310 Size: 9346 2316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2517 5404 9469 9851 Size: 53 83 186 936 984 2887 4065 382 Cuts at: 9851 10486 275 Size: 635 1451 Fragments arranged by size: 4065 2887 1451 984 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2235 3784 8771 10095 10493 10767 10914 11325 2235 Size: 1549 4987 1324 398 274 147 411 2572 Fragments arranged by size: 4987 2572 1549 1324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2764 2794 3860 3890 6204 Size: 30 165 30 1361 30 1066 30 2314 Cuts at: 6204 6234 7256 7286 8270 8300 1178 Size: 30 1022 30 984 30 4540 Fragments arranged by size: 4540 2314 1361 1066 1022 984 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2894 3013 3046 3820 3996 4190 4538 Size: 1255 453 119 33 774 176 194 348 Cuts at: 4538 4994 5424 5807 6078 8517 8519 1186 Size: 456 430 383 271 2439 2 4329 Fragments arranged by size: 4329 2439 1255 774 456 453 430 383 348 271 194 176 119 33 2 BseYI C'CCAG_C Cuts at: 8549 8888 10865 11361 8549 Size: 339 1977 496 8850 Fragments arranged by size: 8850 1977 496 339 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 3056 7354 8739 1163 Size: 573 729 591 4298 1385 4086 Fragments arranged by size: 4298 4086 1385 729 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9112 9873 10901 1540 Size: 275 186 7111 761 1028 2301 Fragments arranged by size: 7111 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3584 4507 4917 7095 8527 9723 9808 10879 3584 Size: 923 410 2178 1432 1196 85 1071 4367 Fragments arranged by size: 4367 2178 1432 1196 1071 923 410 85 BsmI GAATG_Cn' Cuts at: 2496 2675 3697 5479 6673 7153 8293 8468 8799 Size: 179 1022 1782 1194 480 1140 175 331 Cuts at: 8799 9173 9266 2496 Size: 374 93 4892 Fragments arranged by size: 4892 1782 1194 1140 1022 480 374 331 179 175 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3584 4152 4507 4660 4917 5193 6938 Size: 399 1794 568 355 153 257 276 1745 Cuts at: 6938 7095 8527 8981 9723 9808 10879 1391 Size: 157 1432 454 742 85 1071 2174 Fragments arranged by size: 2174 1794 1745 1432 1071 742 568 454 399 355 276 257 157 153 85 BspEI T'CCGG_A Cuts at: 2235 8771 11325 2235 Size: 6536 2554 2572 Fragments arranged by size: 6536 2572 2554 BspHI T'CATG_A Cuts at: 3064 6604 8356 8932 9551 11281 3064 Size: 3540 1752 576 619 1730 3445 Fragments arranged by size: 3540 3445 1752 1730 619 576 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5223 7275 9017 844 Size: 4379 2052 1742 3489 Fragments arranged by size: 4379 3489 2052 1742 BsrBI CCG'CTC Cuts at: 1394 1823 9549 1394 Size: 429 7726 3507 Fragments arranged by size: 7726 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2136 3167 3232 4885 6826 7468 8411 Size: 1183 888 1031 65 1653 1941 642 943 Cuts at: 8411 8568 8879 10157 10331 65 Size: 157 311 1278 174 1396 Fragments arranged by size: 1941 1653 1396 1278 1183 1031 943 888 642 311 174 157 65 BsrFI r'CCGG_y Cuts at: 1700 1810 2583 8301 10306 10493 1700 Size: 110 773 5718 2005 187 2869 Fragments arranged by size: 5718 2869 2005 773 187 110 BsrGI T'GTAC_A Cuts at: 96 2308 4800 5868 96 Size: 2212 2492 1068 5890 Fragments arranged by size: 5890 2492 2212 1068 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11662 BssSI C'ACGA_G Cuts at: 9722 10734 9722 Size: 1012 10650 BstAPI GCAn_nnn'nTGC Cuts at: 794 2509 794 Size: 1715 9947 BstBI TT'CG_AA Cuts at: 2679 10466 2679 Size: 7787 3875 BstEII G'GTnAC_C Cuts at: 1184 8377 1184 Size: 7193 4469 BstYI r'GATC_y Cuts at: 1972 2053 3292 3305 5169 5813 6016 6490 6826 Size: 81 1239 13 1864 644 203 474 336 Cuts at: 6826 6904 7134 7666 8992 9359 9744 9761 11213 Size: 78 230 532 1326 367 385 17 1452 Cuts at: 11213 11299 11311 11657 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1864 1452 1326 1239 644 532 474 385 367 346 336 230 203 86 81 78 17 13 12 BstZ17I GTA'TAC Cuts at: 4815 4815 Size: 11662 Bsu36I CC'TnA_GG Cuts at: 2036 2036 Size: 11662 BtgI C'CryG_G Cuts at: 513 1065 2058 2079 4398 5806 513 Size: 552 993 21 2319 1408 6369 Fragments arranged by size: 6369 2319 1408 993 552 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7209 487 Size: 683 56 5983 4940 Fragments arranged by size: 5983 4940 683 56 BtsI GCAGTG_nn' Cuts at: 2448 2869 3594 3754 3899 6094 6165 6769 6874 Size: 421 725 160 145 2195 71 604 105 Cuts at: 6874 7090 7510 7926 9178 9972 9992 2448 Size: 216 420 416 1252 794 20 4118 Fragments arranged by size: 4118 2195 1252 794 725 604 421 420 416 216 160 145 105 71 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11627 DraI TTT'AAA Cuts at: 6333 6754 8966 6333 Size: 421 2212 9029 Fragments arranged by size: 9029 2212 421 DraIII CAC_nnn'GTG Cuts at: 2443 3388 2443 Size: 945 10717 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10669 809 Size: 507 9353 1802 Fragments arranged by size: 9353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 7012 9109 Size: 54 1425 50 275 18 252 4930 2097 Cuts at: 9109 9998 11627 8 Size: 889 1629 43 Fragments arranged by size: 4930 2097 1629 1425 889 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9109 1537 Size: 275 7297 4090 Fragments arranged by size: 7297 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2118 2562 2946 3507 4126 4833 5992 6984 Size: 765 444 384 561 619 707 1159 992 Cuts at: 6984 7296 8388 9592 1353 Size: 312 1092 1204 3423 Fragments arranged by size: 3423 1204 1159 1092 992 765 707 619 561 444 384 312 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10249 10623 10769 387 Size: 1064 138 8660 374 146 1280 Fragments arranged by size: 8660 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3582 4915 7093 3582 Size: 1333 2178 8151 Fragments arranged by size: 8151 2178 1333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2586 2973 4481 4595 5273 6705 6758 Size: 393 954 387 1508 114 678 1432 53 Cuts at: 6758 7258 7317 7318 8802 9725 10322 11109 11377 Size: 500 59 1 1484 923 597 787 268 Cuts at: 11377 1239 Size: 1524 Fragments arranged by size: 1524 1508 1484 1432 954 923 787 678 597 500 393 387 268 114 59 53 1 EcoNI CCTnn'n_nnAGG Cuts at: 2248 7420 2248 Size: 5172 6490 EcoO109I rG'GnC_Cy Cuts at: 2047 4149 5776 6168 2047 Size: 2102 1627 392 7541 Fragments arranged by size: 7541 2102 1627 392 EcoRI G'AATT_C Cuts at: 4027 4373 6079 7597 8971 9349 4027 Size: 346 1706 1518 1374 378 6340 Fragments arranged by size: 6340 1706 1518 1374 378 346 EcoRV GAT'ATC Cuts at: 6973 6973 Size: 11662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3297 6804 7021 8936 3297 Size: 3507 217 1915 6023 Fragments arranged by size: 6023 3507 1915 217 FspI TGC'GCA Cuts at: 6052 10168 6052 Size: 4116 7546 HaeII r_GCGC'y Cuts at: 1678 10809 1678 Size: 9131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2720 2752 2834 Size: 32 135 32 557 32 559 32 82 Cuts at: 2834 2866 2870 2902 3975 4007 4103 4135 4163 Size: 32 4 32 1073 32 96 32 28 Cuts at: 4163 4195 4208 4240 4585 4617 4772 4804 4925 Size: 32 13 32 345 32 155 32 121 Cuts at: 4925 4957 5154 5186 5204 5236 5452 5484 6509 Size: 32 197 32 18 32 216 32 1025 Cuts at: 6509 6541 6732 6764 7019 7051 7079 7111 7256 Size: 32 191 32 255 32 28 32 145 Cuts at: 7256 7288 7316 7348 7501 7533 10303 10335 10377 Size: 32 28 32 153 32 2770 32 42 Cuts at: 10377 10409 1373 Size: 32 2626 Fragments arranged by size: 2770 2626 1073 1025 559 557 345 255 216 197 191 155 153 145 135 121 96 82 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 28 28 18 13 4 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2419 6223 6804 9006 9252 Size: 988 281 72 409 3804 581 2202 246 Cuts at: 9252 669 Size: 3079 Fragments arranged by size: 3804 3079 2202 988 581 409 281 246 72 HindIII A'AGCT_T Cuts at: 748 2556 3959 5067 5753 6266 8747 748 Size: 1808 1403 1108 686 513 2481 3663 Fragments arranged by size: 3663 2481 1808 1403 1108 686 513 HpaI GTT'AAC Cuts at: 2010 6223 9252 2010 Size: 4213 3029 4420 Fragments arranged by size: 4420 4213 3029 KpnI G_GTAC'C Cuts at: 8987 8987 Size: 11662 MfeI C'AATT_G Cuts at: 3704 9261 3704 Size: 5557 6105 MluI A'CGCG_T Cuts at: 11538 11538 Size: 11662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3668 3924 4073 5755 6304 8268 8656 10776 Size: 2363 256 149 1682 549 1964 388 2120 Cuts at: 10776 10960 1305 Size: 184 2007 Fragments arranged by size: 2363 2120 2007 1964 1682 549 388 256 184 149 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 7014 10 Size: 54 1425 343 252 4930 4658 Fragments arranged by size: 4930 4658 1425 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2090 2304 3066 5537 5942 6659 Size: 648 852 72 214 762 2471 405 717 Cuts at: 6659 7376 7611 8198 8361 9620 9979 10138 518 Size: 717 235 587 163 1259 359 159 2042 Fragments arranged by size: 2471 2042 1259 852 762 717 717 648 587 405 359 235 214 163 159 72 MspA1I CmG'CkG Cuts at: 1606 4644 5251 5497 7058 8314 8618 9078 9755 Size: 3038 607 246 1561 1256 304 460 677 Cuts at: 9755 10903 11148 1606 Size: 1148 245 2120 Fragments arranged by size: 3038 2120 1561 1256 1148 677 607 460 304 246 245 NaeI GCC'GGC Cuts at: 2585 2585 Size: 11662 NcoI C'CATG_G Cuts at: 513 1065 2058 2079 4398 5806 513 Size: 552 993 21 2319 1408 6369 Fragments arranged by size: 6369 2319 1408 993 552 21 NdeI CA'TA_TG Cuts at: 387 4334 7672 387 Size: 3947 3338 4377 Fragments arranged by size: 4377 3947 3338 NgoMIV G'CCGG_C Cuts at: 2583 2583 Size: 11662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11662 NotI GC'GGCC_GC Cuts at: 9109 9109 Size: 11662 NsiI A_TGCA'T Cuts at: 7153 8958 7153 Size: 1805 9857 NspI r_CATG'y Cuts at: 1132 1229 2303 4585 5911 6057 6485 6664 8586 Size: 97 1074 2282 1326 146 428 179 1922 Cuts at: 8586 9020 10565 1132 Size: 434 1545 2229 Fragments arranged by size: 2282 2229 1922 1545 1326 1074 434 428 179 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 11662 PciI A'CATG_T Cuts at: 10561 10561 Size: 11662 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 11662 PfoI T'CCnGG_A Cuts at: 5172 7137 5172 Size: 1965 9697 PmeI GTTT'AAAC Cuts at: 8966 8966 Size: 11662 PmlI CAC'GTG Cuts at: 5210 5210 Size: 11662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9731 9763 11244 11276 9731 Size: 32 1481 32 10117 Fragments arranged by size: 10117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 5776 6168 2047 Size: 3729 392 7541 Fragments arranged by size: 7541 3729 392 PshAI GACnn'nnGTC Cuts at: 1613 2694 1613 Size: 1081 10581 PsiI TTA'TAA Cuts at: 3141 9232 3141 Size: 6091 5571 PspOMI G'GGCC_C Cuts at: 4148 4148 Size: 11662 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2301 2333 5332 5364 2301 Size: 32 2999 32 8599 Fragments arranged by size: 8599 2999 32 32 PstI C_TGCA'G Cuts at: 830 2514 2947 4620 8545 9014 830 Size: 1684 433 1673 3925 469 3478 Fragments arranged by size: 3925 3478 1684 1673 469 433 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11662 PvuII CAG'CTG Cuts at: 4644 5251 5497 8314 8618 4644 Size: 607 246 2817 304 7688 Fragments arranged by size: 7688 2817 607 304 246 SacI G_AGCT'C Cuts at: 3584 4917 7095 3584 Size: 1333 2178 8151 Fragments arranged by size: 8151 2178 1333 SalI G'TCGA_C Cuts at: 1936 9004 1936 Size: 7068 4594 SanDI GG'GwC_CC Cuts at: 6168 6168 Size: 11662 SapI GCTCTTCn'nnn_ Cuts at: 2946 7296 2946 Size: 4350 7312 SbfI CC_TGCA'GG Cuts at: 9014 9014 Size: 11662 ScaI AGT'ACT Cuts at: 9910 11622 9910 Size: 1712 9950 SexAI A'CCwGG_T Cuts at: 5862 7976 8779 5862 Size: 2114 803 8745 Fragments arranged by size: 8745 2114 803 SfcI C'TryA_G Cuts at: 826 1045 2510 2692 2776 2943 4407 4616 6549 Size: 219 1465 182 84 167 1464 209 1933 Cuts at: 6549 8541 9010 10145 10826 11017 826 Size: 1992 469 1135 681 191 1471 Fragments arranged by size: 1992 1933 1471 1465 1464 1135 681 469 219 209 191 182 167 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11662 SmaI CCC'GGG Cuts at: 1872 8989 1872 Size: 7117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2889 3665 3914 4471 4508 4544 Size: 197 926 946 776 249 557 37 36 Cuts at: 4544 4793 9766 10667 10929 820 Size: 249 4973 901 262 1553 Fragments arranged by size: 4973 1553 946 926 901 776 557 262 249 249 197 37 36 SnaBI TAC'GTA Cuts at: 493 9453 493 Size: 8960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11662 SphI G_CATG'C Cuts at: 1132 6664 9020 1132 Size: 5532 2356 3774 Fragments arranged by size: 5532 3774 2356 SspI AAT'ATT Cuts at: 5 52 4000 5833 7537 7699 9586 5 Size: 47 3948 1833 1704 162 1887 2081 Fragments arranged by size: 3948 2081 1887 1833 1704 162 47 StuI AGG'CCT Cuts at: 1517 3872 8477 1517 Size: 2355 4605 4702 Fragments arranged by size: 4702 4605 2355 StyI C'CwwG_G Cuts at: 513 1065 2058 2079 3618 4398 4731 5158 5806 Size: 552 993 21 1539 780 333 427 648 Cuts at: 5806 6424 9101 513 Size: 618 2677 3074 Fragments arranged by size: 3074 2677 1539 993 780 648 618 552 427 333 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 2113 9689 9706 9859 10044 10489 Size: 1255 512 21 7576 17 153 185 445 Cuts at: 10489 325 Size: 1498 Fragments arranged by size: 7576 1498 1255 512 445 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 4800 5868 Size: 274 80 33 51 1443 331 2492 1068 Cuts at: 5868 8170 9908 11620 96 Size: 2302 1738 1712 138 Fragments arranged by size: 2492 2302 1738 1712 1443 1068 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2764 2796 7615 7647 8670 8702 11622 11654 2764 Size: 32 4819 32 1023 32 2920 32 2772 Fragments arranged by size: 4819 2920 2772 1023 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11662 XbaI T'CTAG_A Cuts at: 8998 8998 Size: 11662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3316 6298 8708 3316 Size: 2982 2410 6270 Fragments arranged by size: 6270 2982 2410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11662 XmaI C'CCGG_G Cuts at: 1870 8987 1870 Size: 7117 4545 XmnI GAAnn'nnTTC Cuts at: 4377 6784 6964 7707 7952 8919 9791 4377 Size: 2407 180 743 245 967 872 6248 Fragments arranged by size: 6248 2407 967 872 743 245 180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5405 9470 276 Size: 53 83 186 936 3871 4065 2468 Fragments arranged by size: 4065 3871 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BsiWI BsmBI BstXI FseI FspAI KasI NarI NruI PacI PspXI RsrII SacII SfiI SfoI SrfI SwaI