Restriction map for
pFN21AA0303
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# NO CUT ENZYMES ( 46 enzymes) #
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AatII AflII AhdI AscI AspEI
AspI AvrII BbrPI BclI BfrI
BlnI BsaAI BsiWI BspLU11I Bst1107I
BstZ17I DraI DrdI Eam1105I EcoT22I
FbaI HincII HindII HpaI MfeI
MluI MunI NotI NruI NsiI
PacI PflFI PmaCI PmeI PmlI
PshAI SacI SalI SbfI SexAI
SfiI SgrAI SnaBI Sse8387I SwaI
Tth111I
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 3302 GGTACC G^GTACC
AclI 622 AACGTT AA^CGTT
AflIII 3692 ACGTGT A^C[GA][CT]GT
Alw44I 2725 GTGCAC G^TGCAC
ApaLI 2725 GTGCAC G^TGCAC
AseI 1219 ATTAAT AT^TAAT
AsnI 1219 ATTAAT AT^TAAT
Asp718I 3302 GGTACC G^GTACC
AviII 5769 TGCGCA TGC^GCA
BamHI 996 GGATCC G^GATCC
BcgI 5166 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1773 GGATAC GTATCC(6/5)
BsaBI 2640 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4363 GAATGC GAATGC(1/-1)
Bsp1407I 1485 TGTACA T^GTACA
BspHI 777 TCATGA T^CATGA
BsrGI 1485 TGTACA T^GTACA
BssHII 4056 GCGCGC G^CGCGC
BssSI 2582 CACGAG CACGAG(-5/-1)
CpoI 3307 CGGTCCG CG^G[AT]CCG
EagI 6299 CGGCCG C^GGCCG
EclXI 6299 CGGCCG C^GGCCG
Eco52I 6299 CGGCCG C^GGCCG
EcoRV 1276 GATATC GAT^ATC
FseI 6381 GGCCGGCC GGCCGG^CC
FspI 5769 TGCGCA TGC^GCA
KpnI 3302 GGTACC GGTAC^C
KspI 6437 CCGCGG CCGC^GG
MamI 2640 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 3024 GCTAGC G^CTAGC
PshBI 1219 ATTAAT AT^TAAT
Psp1406I 622 AACGTT AA^CGTT
PvuI 4616 CGATCG CGAT^CG
RcaI 777 TCATGA T^CATGA
RsrII 3307 CGGTCCG CG^G[AT]CCG
SacII 6437 CCGCGG CCGC^GG
ScaI 3970 AGTACT AGT^ACT
SpeI 819 ACTAGT A^CTAGT
SspBI 1485 TGTACA T^GTACA
XbaI 6060 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 541 GTCTAC GT^[AC][GT]AC
1168 GTCTAC
Aor51HI 5168 AGCGCT AGC^GCT
5469 AGCGCT
BbsI 2092 GAAGAC GAAGAC(2/6)
2197 GAAGAC
BglI 1156 GCCTATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4106 GCCAGGGTGGC
BglII 2790 AGATCT A^GATCT
3007 AGATCT
BlpI 3711 GCTCAGC GC^T[ACGT]AGC
6730 GCTCAGC
Bpu1102I 3711 GCTCAGC GC^T[ACGT]AGC
6730 GCTCAGC
BpuAI 2092 GAAGAC GAAGAC(2/6)
2197 GAAGAC
BspDI 1937 ATCGAT AT^CGAT
4273 ATCGAT
BspMI 4826 ACCTGC ACCTGC(4/8)
5032 ACCTGC
CelII 3711 GCTCAGC GC^T[ACGT]AGC
6730 GCTCAGC
ClaI 1937 ATCGAT AT^CGAT
4273 ATCGAT
DraIII 3493 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
6246 CACCCTGTG
Eco47III 5168 AGCGCT AGC^GCT
5469 AGCGCT
EcoNI 1593 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4020 CCTGCTGAAGG
NdeI 1919 CATATG CA^TATG
2056 CATATG
PvuII 4349 CAGCTG CAG^CTG
5405 CAGCTG
SapI 4563 GAAGAGC GCTCTTC(1/4)
4033 GCTCTTC
SmaI 3886 CCCGGG CCC^GGG
5814 CCCGGG
SphI 2764 GCATGC GCATG^C
6686 GCATGC
SspI 1463 AATATT AAT^ATT
2264 AATATT
XmaCI 3886 CCCGGG C^CCGGG
5814 CCCGGG
XmaI 3886 CCCGGG C^CCGGG
5814 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AgeI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstBI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI NspV PaeR7I
PflMI PinAI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SfuI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoI XhoII XmnI