(Circular) (Six-base) MAPSORT of: pFN21AA0303.seq Check: 2263 from: 1 to: 11791 pFN21AA0303 11791 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9601 278 Size: 53 83 186 936 8065 2468 Fragments arranged by size: 8065 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2547 3174 9134 1937 Size: 610 627 5960 4594 Fragments arranged by size: 5960 4594 627 610 Acc65I G'GTAC_C Cuts at: 5307 9112 5307 Size: 3805 7986 AclI AA'CG_TT Cuts at: 1563 2628 9918 10291 1563 Size: 1065 7290 373 3063 Fragments arranged by size: 7290 3063 1065 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4290 4467 4805 4886 5837 6050 6112 6359 Size: 2658 177 338 81 951 213 62 247 Cuts at: 6359 6635 7119 8367 9854 11238 11506 1632 Size: 276 484 1248 1487 1384 268 1917 Fragments arranged by size: 2658 1917 1487 1384 1248 951 484 338 276 268 247 213 177 81 62 AfeI AGC'GCT Cuts at: 7175 7476 7175 Size: 301 11490 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11594 AflIII A'CryG_T Cuts at: 1203 5697 10690 11667 1203 Size: 4494 4993 977 1327 Fragments arranged by size: 4993 4494 1327 977 AgeI A'CCGG_T Cuts at: 5310 7848 8415 10622 5310 Size: 2538 567 2207 6479 Fragments arranged by size: 6479 2538 2207 567 AhdI GACnn_n'nnGTC Cuts at: 10520 10520 Size: 11791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 11791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3225 4250 1184 Size: 2041 1025 8725 Fragments arranged by size: 8725 2041 1025 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6830 6862 6830 Size: 32 11759 AlwNI CAG_nnn'CTG Cuts at: 3868 4313 4625 5686 6695 7235 8179 8552 9534 Size: 445 312 1061 1009 540 944 373 982 Cuts at: 9534 11106 3868 Size: 1572 4553 Fragments arranged by size: 4553 1572 1061 1009 982 944 540 445 373 312 ApaI G_GGCC'C Cuts at: 6139 7826 8034 8487 6139 Size: 1687 208 453 9443 Fragments arranged by size: 9443 1687 453 208 ApaLI G'TGCA_C Cuts at: 4730 9848 11004 4730 Size: 5118 1156 5517 Fragments arranged by size: 5517 5118 1156 ApoI r'AATT_y Cuts at: 1493 2212 2882 2897 3746 3992 4183 6874 7416 Size: 719 670 15 849 246 191 2691 542 Cuts at: 7416 9100 9326 9478 11587 1493 Size: 1684 226 152 2109 1697 Fragments arranged by size: 2691 2109 1697 1684 849 719 670 542 246 226 191 152 15 AseI AT'TA_AT Cuts at: 160 1032 3225 10345 160 Size: 872 2193 7120 1606 Fragments arranged by size: 7120 2193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11791 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3117 5891 7741 7819 8293 8381 Size: 73 745 429 2774 1850 78 474 88 Cuts at: 8381 9116 1870 Size: 735 4545 Fragments arranged by size: 4545 2774 1850 745 735 474 429 88 78 73 BamHI G'GATC_C Cuts at: 3001 9121 9488 3001 Size: 6120 367 5304 Fragments arranged by size: 6120 5304 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3342 3844 5307 5671 6007 Size: 325 217 627 1555 502 1463 364 336 Cuts at: 6007 6094 6149 7330 7565 9112 10567 618 Size: 87 55 1181 235 1547 1455 1842 Fragments arranged by size: 1842 1555 1547 1463 1455 1181 627 502 364 336 325 235 217 87 55 BanII G_rGCy'C Cuts at: 1391 2495 5526 6139 6417 6575 6707 6950 7209 Size: 1104 3031 613 278 158 132 243 259 Cuts at: 7209 7737 7748 7826 8034 8062 8487 8872 9110 Size: 528 11 78 208 28 425 385 238 Cuts at: 9110 1391 Size: 4072 Fragments arranged by size: 4072 3031 1104 613 528 425 385 278 259 243 238 208 158 132 78 28 11 BbeI G_GCGC'C Cuts at: 3346 5675 6011 7334 3346 Size: 2329 336 1323 7803 Fragments arranged by size: 7803 2329 1323 336 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4104 4209 928 Size: 971 2205 105 8510 Fragments arranged by size: 8510 2205 971 105 BbvCI CC'TCA_GC Cuts at: 2230 4615 8545 2230 Size: 2385 3930 5476 Fragments arranged by size: 5476 3930 2385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7160 7194 9982 10016 1903 Size: 34 5223 34 2788 34 3678 Fragments arranged by size: 5223 3678 2788 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3772 9683 10893 3772 Size: 5911 1210 4670 Fragments arranged by size: 5911 4670 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 11791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3167 6117 10402 Size: 107 122 71 631 605 1495 2950 4285 Cuts at: 10402 136 Size: 1525 Fragments arranged by size: 4285 2950 1525 1495 631 605 122 107 71 BglII A'GATC_T Cuts at: 4795 5012 11786 4795 Size: 217 6774 4800 Fragments arranged by size: 6774 4800 217 BlpI GC'TnA_GC Cuts at: 5717 8736 9208 5717 Size: 3019 472 8300 Fragments arranged by size: 8300 3019 472 Bme1580I G_kGCm'C Cuts at: 1790 3540 4459 4734 5230 5256 6139 6632 7826 Size: 1750 919 275 496 26 883 493 1194 Cuts at: 7826 8034 8487 9852 11008 1790 Size: 208 453 1365 1156 2573 Fragments arranged by size: 2573 1750 1365 1194 1156 919 883 496 493 453 275 208 26 BmgBI CAC'GTC Cuts at: 1318 5707 6724 8965 1318 Size: 4389 1017 2241 4144 Fragments arranged by size: 4389 4144 2241 1017 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 2996 5441 Size: 416 53 83 399 24 800 779 2445 Cuts at: 5441 5753 6380 8562 9522 10480 442 Size: 312 627 2182 960 958 1753 Fragments arranged by size: 2445 2182 1753 960 958 800 779 627 416 399 312 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5033 1056 Size: 3977 7814 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5031 6238 7009 7756 5031 Size: 1207 771 747 9066 Fragments arranged by size: 9066 1207 771 747 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3768 3920 4279 4425 4812 5645 6140 6854 Size: 2529 152 359 146 387 833 495 714 Cuts at: 6854 6857 7522 8126 8135 8346 8593 8717 8834 Size: 3 665 604 9 211 247 124 117 Cuts at: 8834 10451 1239 Size: 1617 2579 Fragments arranged by size: 2579 2529 1617 833 714 665 604 495 387 359 247 211 152 146 124 117 9 3 Bpu10I CC'TnA_GC Cuts at: 2230 2638 4615 8545 2230 Size: 408 1977 3930 5476 Fragments arranged by size: 5476 3930 1977 408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2181 5528 6326 6479 7096 9916 10781 11079 2181 Size: 3347 798 153 617 2820 865 298 2893 Fragments arranged by size: 3347 2893 2820 865 798 617 298 153 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2335 4401 5151 6170 6872 7497 8803 Size: 617 836 2066 750 1019 702 625 1306 Cuts at: 8803 10454 882 Size: 1651 2219 Fragments arranged by size: 2219 2066 1651 1306 1019 836 750 702 625 617 BsaAI yAC'GTr Cuts at: 493 1204 9582 493 Size: 711 8378 2702 Fragments arranged by size: 8378 2702 711 BsaBI GATnn'nnATC Cuts at: 4649 11785 4649 Size: 7136 4655 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2266 3343 5366 5672 Size: 53 83 186 936 733 1077 2023 306 Cuts at: 5672 6008 7331 8070 9598 9980 10615 275 Size: 336 1323 739 1528 382 635 1451 Fragments arranged by size: 2023 1528 1451 1323 1077 936 739 733 635 382 336 306 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 2890 5310 5315 6653 7848 8415 10224 10622 Size: 778 2420 5 1338 1195 567 1809 398 Cuts at: 10622 10896 11043 11454 2112 Size: 274 147 411 2449 Fragments arranged by size: 2449 2420 1809 1338 1195 778 567 411 398 274 147 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4809 4839 5356 5386 5555 Size: 30 165 30 3406 30 517 30 169 Cuts at: 5555 5585 5591 5621 6732 6762 6830 6860 8194 Size: 30 6 30 1111 30 68 30 1334 Cuts at: 8194 8224 1178 Size: 30 4745 Fragments arranged by size: 4745 3406 1334 1111 517 169 165 68 30 30 30 30 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 3803 3872 4433 4478 5023 5576 5654 Size: 894 1723 69 561 45 545 553 78 Cuts at: 5654 7256 7387 7849 8052 8596 1186 Size: 1602 131 462 203 544 4381 Fragments arranged by size: 4381 1723 1602 894 561 553 545 544 462 203 131 78 69 45 BseYI C'CCAG_C Cuts at: 2318 3907 4483 4601 5239 5264 5462 5661 6016 Size: 1589 576 118 638 25 198 199 355 Cuts at: 6016 6707 6752 6857 7435 7610 7721 7736 8711 Size: 691 45 105 578 175 111 15 975 Cuts at: 8711 8920 8925 8935 8980 10994 11490 2318 Size: 209 5 10 45 2014 496 2619 Fragments arranged by size: 2619 2014 1589 975 691 638 578 576 496 355 209 199 198 175 118 111 105 45 45 25 15 10 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5573 5666 6681 7388 8030 1163 Size: 573 3837 93 1015 707 642 4924 Fragments arranged by size: 4924 3837 1015 707 642 573 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6624 7849 8307 9241 10002 11030 Size: 275 186 4623 1225 458 934 761 1028 Cuts at: 11030 1540 Size: 2301 Fragments arranged by size: 4623 2301 1225 1028 934 761 458 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 4734 5609 6108 9852 9937 11008 2040 Size: 2694 875 499 3744 85 1071 2823 Fragments arranged by size: 3744 2823 2694 1071 875 499 85 BsmI GAATG_Cn' Cuts at: 6374 9302 9395 6374 Size: 2928 93 8770 Fragments arranged by size: 8770 2928 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3443 6484 8955 3443 Size: 3041 2471 6279 Fragments arranged by size: 6279 3041 2471 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2495 3540 4459 4734 5230 5256 Size: 399 250 455 1045 919 275 496 26 Cuts at: 5256 5526 5609 6108 6139 6417 6575 6632 6707 Size: 270 83 499 31 278 158 57 75 Cuts at: 6707 6950 7209 7737 7748 7826 8034 8062 8487 Size: 243 259 528 11 78 208 28 425 Cuts at: 8487 8872 9110 9852 9937 11008 1391 Size: 385 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 1045 919 742 528 499 496 455 425 399 385 278 275 270 259 250 243 238 208 158 85 83 78 75 57 31 28 26 11 BspEI T'CCGG_A Cuts at: 2890 5315 6653 11454 2890 Size: 2425 1338 4801 3227 Fragments arranged by size: 4801 3227 2425 1338 BspHI T'CATG_A Cuts at: 2782 9680 11410 2782 Size: 6898 1730 3163 Fragments arranged by size: 6898 3163 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6840 7046 9146 844 Size: 5996 206 2100 3489 Fragments arranged by size: 5996 3489 2100 206 BsrBI CCG'CTC Cuts at: 1394 1823 3277 5166 5716 6143 7090 8434 9678 Size: 429 1454 1889 550 427 947 1344 1244 Cuts at: 9678 1394 Size: 3507 Fragments arranged by size: 3507 1889 1454 1344 1244 947 550 429 427 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 2147 3160 3669 6639 7672 10286 Size: 1183 889 10 1013 509 2970 1033 2614 Cuts at: 10286 10460 65 Size: 174 1396 Fragments arranged by size: 2970 2614 1396 1183 1033 1013 889 509 174 10 BsrFI r'CCGG_y Cuts at: 1700 1810 3709 5310 5333 6421 7848 8302 8387 Size: 110 1899 1601 23 1088 1427 454 85 Cuts at: 8387 8415 10435 10622 1700 Size: 28 2020 187 2869 Fragments arranged by size: 2869 2020 1899 1601 1427 1088 454 187 110 85 28 23 BsrGI T'GTAC_A Cuts at: 96 3490 96 Size: 3394 8397 BssHII G'CGCG_C Cuts at: 1925 6061 1925 Size: 4136 7655 BssSI C'ACGA_G Cuts at: 4587 9851 10863 4587 Size: 5264 1012 5515 Fragments arranged by size: 5515 5264 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3324 4625 8357 794 Size: 2530 1301 3732 4228 Fragments arranged by size: 4228 3732 2530 1301 BstBI TT'CG_AA Cuts at: 2830 3106 3247 10595 2830 Size: 276 141 7348 4026 Fragments arranged by size: 7348 4026 276 141 BstEII G'GTnAC_C Cuts at: 1184 3523 4474 5403 5629 1184 Size: 2339 951 929 226 7346 Fragments arranged by size: 7346 2339 951 929 226 BstXI CCAn_nnnn'nTGG Cuts at: 5830 7358 7697 5830 Size: 1528 339 9924 Fragments arranged by size: 9924 1528 339 BstYI r'GATC_y Cuts at: 1972 2835 3001 3255 3865 4795 5012 5087 9121 Size: 863 166 254 610 930 217 75 4034 Cuts at: 9121 9488 9873 9890 11342 11428 11440 11786 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 4034 1977 1452 930 863 610 385 367 346 254 217 166 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 3884 4470 6660 7130 8466 3884 Size: 586 2190 470 1336 7209 Fragments arranged by size: 7209 2190 1336 586 470 BtgI C'CryG_G Cuts at: 513 1065 2004 3531 3726 5275 5884 6396 7212 Size: 552 939 1527 195 1549 609 512 816 Cuts at: 7212 7314 8442 8574 8841 513 Size: 102 1128 132 267 3463 Fragments arranged by size: 3463 1549 1527 1128 939 816 609 552 512 267 195 132 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2419 6092 8019 487 Size: 683 56 1193 3673 1927 4259 Fragments arranged by size: 4259 3673 1927 1193 683 56 BtsI GCAGTG_nn' Cuts at: 5593 6766 7725 8042 9307 10101 10121 5593 Size: 1173 959 317 1265 794 20 7263 Fragments arranged by size: 7263 1265 1173 959 794 317 20 ClaI AT'CG_AT Cuts at: 1554 3943 6279 1554 Size: 2389 2336 7066 Fragments arranged by size: 7066 2389 2336 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11756 DraI TTT'AAA Cuts at: 9095 9095 Size: 11791 DraIII CAC_nnn'GTG Cuts at: 5503 8256 5503 Size: 2753 9038 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 10798 809 Size: 507 9482 1802 Fragments arranged by size: 9482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3429 5729 6270 Size: 54 1425 50 275 18 1599 2300 541 Cuts at: 6270 7071 7638 8014 8304 8435 9238 10127 11756 Size: 801 567 376 290 131 803 889 1629 Cuts at: 11756 8 Size: 43 Fragments arranged by size: 2300 1629 1599 1425 889 803 801 567 541 376 290 275 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8304 9238 1537 Size: 275 6492 934 4090 Fragments arranged by size: 6492 4090 934 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2338 4188 4309 4443 4862 5421 5925 6045 Size: 985 1850 121 134 419 559 504 120 Cuts at: 6045 6563 7517 8674 9721 1353 Size: 518 954 1157 1047 3423 Fragments arranged by size: 3423 1850 1157 1047 985 954 559 518 504 419 134 121 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4984 5320 7614 8342 10378 10752 Size: 1064 138 3395 336 2294 728 2036 374 Cuts at: 10752 10898 387 Size: 146 1280 Fragments arranged by size: 3395 2294 2036 1280 1064 728 374 336 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3768 3920 4279 4290 4425 4467 4805 Size: 393 2136 152 359 11 135 42 338 Cuts at: 4805 4812 4886 5645 5837 6050 6112 6140 6359 Size: 7 74 759 192 213 62 28 219 Cuts at: 6359 6635 6854 6857 7119 7522 8126 8135 8346 Size: 276 219 3 262 403 604 9 211 Cuts at: 8346 8367 8593 8717 8834 9854 10451 11238 11506 Size: 21 226 124 117 1020 597 787 268 Cuts at: 11506 1239 Size: 1524 Fragments arranged by size: 2136 1524 1020 787 759 604 597 403 393 359 338 276 268 262 226 219 219 213 211 192 152 135 124 117 74 62 42 28 21 11 9 7 3 EcoNI CCTnn'n_nnAGG Cuts at: 3602 6029 3602 Size: 2427 9364 EcoO109I rG'GnC_Cy Cuts at: 2716 3410 5532 6136 6729 6926 7151 7281 7377 Size: 694 2122 604 593 197 225 130 96 Cuts at: 7377 7753 7823 8031 8153 8483 8911 2716 Size: 376 70 208 122 330 428 5596 Fragments arranged by size: 5596 2122 694 604 593 428 376 330 225 208 197 130 122 96 70 EcoRI G'AATT_C Cuts at: 4183 6874 7416 9100 9478 4183 Size: 2691 542 1684 378 6496 Fragments arranged by size: 6496 2691 1684 542 378 EcoRV GAT'ATC Cuts at: 3283 3283 Size: 11791 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3022 6556 7496 7710 3022 Size: 3534 940 214 7103 Fragments arranged by size: 7103 3534 940 214 FseI GG_CCGG'CC Cuts at: 8391 8391 Size: 11791 FspI TGC'GCA Cuts at: 7776 10297 7776 Size: 2521 9270 HaeII r_GCGC'y Cuts at: 1678 3346 5675 6011 7177 7334 7478 10938 1678 Size: 1668 2329 336 1166 157 144 3460 2531 Fragments arranged by size: 3460 2531 2329 1668 1166 336 157 144 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3792 3824 4028 4060 4317 Size: 32 135 32 2220 32 204 32 257 Cuts at: 4317 4349 4785 4817 5168 5200 5356 5388 5555 Size: 32 436 32 351 32 156 32 167 Cuts at: 5555 5587 6429 6461 7382 7414 10432 10464 10506 Size: 32 842 32 921 32 3018 32 42 Cuts at: 10506 10538 1373 Size: 32 2626 Fragments arranged by size: 3018 2626 2220 921 842 436 351 257 204 167 156 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 9135 9381 669 Size: 988 281 7197 246 3079 Fragments arranged by size: 7197 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2119 7028 7101 748 Size: 1371 4909 73 5438 Fragments arranged by size: 5438 4909 1371 73 HpaI GTT'AAC Cuts at: 9381 9381 Size: 11791 KasI G'GCGC_C Cuts at: 3342 5671 6007 7330 3342 Size: 2329 336 1323 7803 Fragments arranged by size: 7803 2329 1323 336 KpnI G_GTAC'C Cuts at: 5311 9116 5311 Size: 3805 7986 MfeI C'AATT_G Cuts at: 9390 9390 Size: 11791 MluI A'CGCG_T Cuts at: 11667 11667 Size: 11791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3736 4323 5174 5399 6063 6415 10905 11089 Size: 2431 587 851 225 664 352 4490 184 Cuts at: 11089 1305 Size: 2007 Fragments arranged by size: 4490 2431 2007 851 664 587 352 225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3431 6272 7073 8016 10 Size: 54 1425 343 1599 2841 801 943 3785 Fragments arranged by size: 3785 2841 1599 1425 943 801 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2465 3359 3480 4697 4812 7356 Size: 648 843 456 894 121 1217 115 2544 Cuts at: 7356 9749 10108 10267 518 Size: 2393 359 159 2042 Fragments arranged by size: 2544 2393 2042 1217 894 843 648 456 359 159 121 115 MspA1I CmG'CkG Cuts at: 1606 2271 5305 5356 5401 5539 5650 5665 5677 Size: 665 3034 51 45 138 111 15 12 Cuts at: 5677 6016 6356 7412 8168 8186 8444 9207 9884 Size: 339 340 1056 756 18 258 763 677 Cuts at: 9884 11032 11277 1606 Size: 1148 245 2120 Fragments arranged by size: 3034 2120 1148 1056 763 756 677 665 340 339 258 245 138 111 51 45 18 15 12 NaeI GCC'GGC Cuts at: 5335 6423 8304 8389 5335 Size: 1088 1881 85 8737 Fragments arranged by size: 8737 1881 1088 85 NarI GG'CG_CC Cuts at: 3343 5672 6008 7331 3343 Size: 2329 336 1323 7803 Fragments arranged by size: 7803 2329 1323 336 NcoI C'CATG_G Cuts at: 513 1065 2004 3531 3726 5884 7212 7314 8841 Size: 552 939 1527 195 2158 1328 102 1527 Cuts at: 8841 513 Size: 3463 Fragments arranged by size: 3463 2158 1527 1527 1328 939 552 195 102 NdeI CA'TA_TG Cuts at: 387 3925 4062 387 Size: 3538 137 8116 Fragments arranged by size: 8116 3538 137 NgoMIV G'CCGG_C Cuts at: 5333 6421 8302 8387 5333 Size: 1088 1881 85 8737 Fragments arranged by size: 8737 1881 1088 85 NheI G'CTAG_C Cuts at: 1052 5029 1052 Size: 3977 7814 NotI GC'GGCC_GC Cuts at: 9238 9238 Size: 11791 NspI r_CATG'y Cuts at: 1132 1229 3322 4773 7200 8695 9149 10694 1132 Size: 97 2093 1451 2427 1495 454 1545 2229 Fragments arranged by size: 2427 2229 2093 1545 1495 1451 454 97 PasI CC'CwG_GG Cuts at: 1348 8150 1348 Size: 6802 4989 PciI A'CATG_T Cuts at: 10690 10690 Size: 11791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 3773 4829 7315 1108 Size: 920 1745 1056 2486 5584 Fragments arranged by size: 5584 2486 1745 1056 920 PfoI T'CCnGG_A Cuts at: 3636 5765 6156 8583 3636 Size: 2129 391 2427 6844 Fragments arranged by size: 6844 2427 2129 391 PmeI GTTT'AAAC Cuts at: 9095 9095 Size: 11791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4246 4278 6830 6862 9860 9892 11373 11405 4246 Size: 32 2552 32 2998 32 1481 32 4632 Fragments arranged by size: 4632 2998 2552 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3410 5532 6926 7281 7753 8911 3410 Size: 2122 1394 355 472 1158 6290 Fragments arranged by size: 6290 2122 1394 1158 472 355 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11791 PsiI TTA'TAA Cuts at: 9361 9361 Size: 11791 PspOMI G'GGCC_C Cuts at: 6135 7822 8030 8483 6135 Size: 1687 208 453 9443 Fragments arranged by size: 9443 1687 453 208 PspXI vC'TCGA_Gb Cuts at: 7741 8381 7741 Size: 640 11151 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2531 2563 2531 Size: 32 11759 PstI C_TGCA'G Cuts at: 830 5682 7500 7732 8653 9143 830 Size: 4852 1818 232 921 490 3478 Fragments arranged by size: 4852 3478 1818 921 490 232 PvuI CG_AT'CG Cuts at: 2001 6624 2001 Size: 4623 7168 PvuII CAG'CTG Cuts at: 6356 7412 6356 Size: 1056 10735 RsrII CG'GwC_CG Cuts at: 5313 5313 Size: 11791 SacII CC_GC'GG Cuts at: 8445 8445 Size: 11791 SalI G'TCGA_C Cuts at: 1936 9133 1936 Size: 7197 4594 SapI GCTCTTCn'nnn_ Cuts at: 6045 6563 6045 Size: 518 11273 SbfI CC_TGCA'GG Cuts at: 9143 9143 Size: 11791 ScaI AGT'ACT Cuts at: 5977 10039 11751 5977 Size: 4062 1712 6017 Fragments arranged by size: 6017 4062 1712 SfcI C'TryA_G Cuts at: 826 1045 2571 2771 5678 7496 7728 8649 9139 Size: 219 1526 200 2907 1818 232 921 490 Cuts at: 9139 10274 10955 11146 826 Size: 1135 681 191 1471 Fragments arranged by size: 2907 1818 1526 1471 1135 921 681 490 232 219 200 191 SfoI GGC'GCC Cuts at: 3344 5673 6009 7332 3344 Size: 2329 336 1323 7803 Fragments arranged by size: 7803 2329 1323 336 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11791 SmaI CCC'GGG Cuts at: 1872 5893 7821 9118 1872 Size: 4021 1928 1297 4545 Fragments arranged by size: 4545 4021 1928 1297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 2688 5543 6305 6494 7111 Size: 197 926 253 492 2855 762 189 617 Cuts at: 7111 7741 8381 9895 10796 11058 820 Size: 630 640 1514 901 262 1553 Fragments arranged by size: 2855 1553 1514 926 901 762 640 630 617 492 262 253 197 189 SnaBI TAC'GTA Cuts at: 493 9582 493 Size: 9089 2702 SpeI A'CTAG_T Cuts at: 152 2824 152 Size: 2672 9119 SphI G_CATG'C Cuts at: 1132 4773 8695 9149 1132 Size: 3641 3922 454 3774 Fragments arranged by size: 3922 3774 3641 454 SspI AAT'ATT Cuts at: 5 52 3470 4271 9715 5 Size: 47 3418 801 5444 2081 Fragments arranged by size: 5444 3418 2081 801 47 StuI AGG'CCT Cuts at: 1517 3266 6500 6665 7128 8645 9086 1517 Size: 1749 3234 165 463 1517 441 4222 Fragments arranged by size: 4222 3234 1749 1517 463 441 165 StyI C'CwwG_G Cuts at: 513 1065 2004 3006 3462 3531 3726 5823 5884 Size: 552 939 1002 456 69 195 2097 61 Cuts at: 5884 6231 6842 7212 7314 7569 8135 8676 8841 Size: 347 611 370 102 255 566 541 165 Cuts at: 8841 9087 9230 513 Size: 246 143 3074 Fragments arranged by size: 3074 2097 1002 939 611 566 552 541 456 370 347 255 246 195 165 143 102 69 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2332 4032 5302 6470 7302 8163 9818 Size: 1255 752 1700 1270 1168 832 861 1655 Cuts at: 9818 9835 9988 10173 10618 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1700 1655 1498 1270 1255 1168 861 832 752 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3438 3490 5975 Size: 274 80 33 51 1443 1461 52 2485 Cuts at: 5975 10037 11749 96 Size: 4062 1712 138 Fragments arranged by size: 4062 2485 1712 1461 1443 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2721 2753 3696 3728 4809 4841 5591 5623 5864 Size: 32 943 32 1081 32 750 32 241 Cuts at: 5864 5896 6732 6764 11751 11783 2721 Size: 32 836 32 4987 32 2729 Fragments arranged by size: 4987 2729 1081 943 836 750 241 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11791 XbaI T'CTAG_A Cuts at: 8065 9127 8065 Size: 1062 10729 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5882 7952 8024 8809 5882 Size: 2070 72 785 8864 Fragments arranged by size: 8864 2070 785 72 XhoI C'TCGA_G Cuts at: 1943 2688 7741 8381 1943 Size: 745 5053 640 5353 Fragments arranged by size: 5353 5053 745 640 XmaI C'CCGG_G Cuts at: 1870 5891 7819 9116 1870 Size: 4021 1928 1297 4545 Fragments arranged by size: 4545 4021 1928 1297 XmnI GAAnn'nnTTC Cuts at: 3684 3996 7476 8459 9920 3684 Size: 312 3480 983 1461 5555 Fragments arranged by size: 5555 3480 1461 983 312 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9599 276 Size: 53 83 186 936 8065 2468 Fragments arranged by size: 8065 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI FspAI NruI NsiI PacI PmlI SacI SanDI SexAI SfiI SrfI SwaI