Restriction map for
pFN21AA0303
(Linear) (Six-base) MAPSORT of: KIAA0303.seq Check: 9854 from: 1 to: 7086
KIAA0303.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AccI GT'mk_AC
Cuts at: 0 542 1169 7086
Size: 542 627 5917
Fragments arranged by size:
5917 627 542
Acc65I G'GTAC_C
Cuts at: 0 3302 7086
Size: 3302 3784
AclI AA'CG_TT
Cuts at: 0 623 7086
Size: 623 6463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2285 2462 2800 2881 3832 4045 4107 4354
Size: 2285 177 338 81 951 213 62 247
Cuts at: 4354 4630 5114 6362 7086
Size: 276 484 1248 724
Fragments arranged by size:
2285 1248 951 724 484 338 276 247
213 177 81 62
AfeI AGC'GCT
Cuts at: 0 5170 5471 7086
Size: 5170 301 1615
Fragments arranged by size:
5170 1615 301
AflIII A'CryG_T
Cuts at: 0 3692 7086
Size: 3692 3394
AgeI A'CCGG_T
Cuts at: 0 3305 5843 6410 7086
Size: 3305 2538 567 676
Fragments arranged by size:
3305 2538 676 567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1220 2245 7086
Size: 1220 1025 4841
Fragments arranged by size:
4841 1220 1025
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4825 4857 7086
Size: 4825 32 2229
Fragments arranged by size:
4825 2229 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1863 2308 2620 3681 4690 5230 6174 6547
Size: 1863 445 312 1061 1009 540 944 373
Cuts at: 6547 7086
Size: 539
Fragments arranged by size:
1863 1061 1009 944 540 539 445 373
312
ApaI G_GGCC'C
Cuts at: 0 4134 5821 6029 6482 7086
Size: 4134 1687 208 453 604
Fragments arranged by size:
4134 1687 604 453 208
ApaLI G'TGCA_C
Cuts at: 0 2725 7086
Size: 2725 4361
ApoI r'AATT_y
Cuts at: 0 207 877 892 1741 1987 2178 4869 5411
Size: 207 670 15 849 246 191 2691 542
Cuts at: 5411 7086
Size: 1675
Fragments arranged by size:
2691 1675 849 670 542 246 207 191
15
AseI AT'TA_AT
Cuts at: 0 1220 7086
Size: 1220 5866
AvaI C'yCGr_G
Cuts at: 0 683 1112 3886 5736 5814 6288 6376 7086
Size: 683 429 2774 1850 78 474 88 710
Fragments arranged by size:
2774 1850 710 683 474 429 88 78
BamHI G'GATC_C
Cuts at: 0 996 7086
Size: 996 6090
BanI G'GyrC_C
Cuts at: 0 1337 1839 3302 3666 4002 4089 4144 5325
Size: 1337 502 1463 364 336 87 55 1181
Cuts at: 5325 5560 7086
Size: 235 1526
Fragments arranged by size:
1526 1463 1337 1181 502 364 336 235
87 55
BanII G_rGCy'C
Cuts at: 0 490 3521 4134 4412 4570 4702 4945 5204
Size: 490 3031 613 278 158 132 243 259
Cuts at: 5204 5732 5743 5821 6029 6057 6482 6867 7086
Size: 528 11 78 208 28 425 385 219
Fragments arranged by size:
3031 613 528 490 425 385 278 259
243 219 208 158 132 78 28 11
BbeI G_GCGC'C
Cuts at: 0 1341 3670 4006 5329 7086
Size: 1341 2329 336 1323 1757
Fragments arranged by size:
2329 1757 1341 1323 336
BbsI GAAGACnn'nnnn_
Cuts at: 0 2099 2204 7086
Size: 2099 105 4882
Fragments arranged by size:
4882 2099 105
BbvCI CC'TCA_GC
Cuts at: 0 225 2610 6540 7086
Size: 225 2385 3930 546
Fragments arranged by size:
3930 2385 546 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5155 5189 7086
Size: 5155 34 1897
Fragments arranged by size:
5155 1897 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1767 7086
Size: 1767 5319
BglI GCCn_nnn'nGGC
Cuts at: 0 1162 4112 7086
Size: 1162 2950 2974
Fragments arranged by size:
2974 2950 1162
BglII A'GATC_T
Cuts at: 0 2790 3007 7086
Size: 2790 217 4079
Fragments arranged by size:
4079 2790 217
BlpI GC'TnA_GC
Cuts at: 0 3712 6731 7086
Size: 3712 3019 355
Fragments arranged by size:
3712 3019 355
Bme1580I G_kGCm'C
Cuts at: 0 1535 2454 2729 3225 3251 4134 4627 5821
Size: 1535 919 275 496 26 883 493 1194
Cuts at: 5821 6029 6482 7086
Size: 208 453 604
Fragments arranged by size:
1535 1194 919 883 604 496 493 453
275 208 26
BmgBI CAC'GTC
Cuts at: 0 3702 4719 6960 7086
Size: 3702 1017 2241 126
Fragments arranged by size:
3702 2241 1017 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 991 3436 3748 4375 6557 7086
Size: 212 779 2445 312 627 2182 529
Fragments arranged by size:
2445 2182 779 627 529 312 212
BmtI G_CTAG'C
Cuts at: 0 3028 7086
Size: 3028 4058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3026 4233 5004 5751 7086
Size: 3026 1207 771 747 1335
Fragments arranged by size:
3026 1335 1207 771 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1763 1915 2274 2420 2807 3640 4135 4849
Size: 1763 152 359 146 387 833 495 714
Cuts at: 4849 4852 5517 6121 6130 6341 6588 6712 6829
Size: 3 665 604 9 211 247 124 117
Cuts at: 6829 7086
Size: 257
Fragments arranged by size:
1763 833 714 665 604 495 387 359
257 247 211 152 146 124 117 9
3
Bpu10I CC'TnA_GC
Cuts at: 0 225 633 2610 6540 7086
Size: 225 408 1977 3930 546
Fragments arranged by size:
3930 1977 546 408 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 3523 4321 4474 5091 7086
Size: 176 3347 798 153 617 1995
Fragments arranged by size:
3347 1995 798 617 176 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 330 2396 3146 4165 4867 5492 6798 7086
Size: 330 2066 750 1019 702 625 1306 288
Fragments arranged by size:
2066 1306 1019 750 702 625 330 288
BsaBI GATnn'nnATC
Cuts at: 0 2644 7086
Size: 2644 4442
BsaHI Gr'CG_yC
Cuts at: 0 261 1338 3361 3667 4003 5326 6065 7086
Size: 261 1077 2023 306 336 1323 739 1021
Fragments arranged by size:
2023 1323 1077 1021 739 336 306 261
BsaWI w'CCGG_w
Cuts at: 0 107 885 3305 3310 4648 5843 6410 7086
Size: 107 778 2420 5 1338 1195 567 676
Fragments arranged by size:
2420 1338 1195 778 676 567 107 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2804 2834 3351 3381 3550 3580 3586 3616
Size: 2804 30 517 30 169 30 6 30
Cuts at: 3616 4727 4757 4825 4855 6189 6219 7086
Size: 1111 30 68 30 1334 30 867
Fragments arranged by size:
2804 1334 1111 867 517 169 68 30
30 30 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 1798 1867 2428 2473 3018 3571 3649
Size: 75 1723 69 561 45 545 553 78
Cuts at: 3649 5251 5382 5844 6047 6591 7086
Size: 1602 131 462 203 544 495
Fragments arranged by size:
1723 1602 561 553 545 544 495 462
203 131 78 75 69 45
BseYI C'CCAG_C
Cuts at: 0 313 1902 2478 2596 3234 3259 3457 3656
Size: 313 1589 576 118 638 25 198 199
Cuts at: 3656 4011 4702 4747 4852 5430 5605 5716 5731
Size: 355 691 45 105 578 175 111 15
Cuts at: 5731 6706 6915 6920 6930 6975 7086
Size: 975 209 5 10 45 111
Fragments arranged by size:
1589 975 691 638 578 576 355 313
209 199 198 175 118 111 111 105
45 45 25 15 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3568 3661 4676 5383 6025 7086
Size: 3568 93 1015 707 642 1061
Fragments arranged by size:
3568 1061 1015 707 642 93
BsiEI CG_ry'CG
Cuts at: 0 4619 5844 6302 7086
Size: 4619 1225 458 784
Fragments arranged by size:
4619 1225 784 458
BsiHKAI G_wGCw'C
Cuts at: 0 35 2729 3604 4103 7086
Size: 35 2694 875 499 2983
Fragments arranged by size:
2983 2694 875 499 35
BsmI GAATG_Cn'
Cuts at: 0 4369 7086
Size: 4369 2717
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1438 4479 6950 7086
Size: 1438 3041 2471 136
Fragments arranged by size:
3041 2471 1438 136
Bsp1286I G_dGCh'C
Cuts at: 0 35 490 1535 2454 2729 3225 3251 3521
Size: 35 455 1045 919 275 496 26 270
Cuts at: 3521 3604 4103 4134 4412 4570 4627 4702 4945
Size: 83 499 31 278 158 57 75 243
Cuts at: 4945 5204 5732 5743 5821 6029 6057 6482 6867
Size: 259 528 11 78 208 28 425 385
Cuts at: 6867 7086
Size: 219
Fragments arranged by size:
1045 919 528 499 496 455 425 385
278 275 270 259 243 219 208 158
83 78 75 57 35 31 28 26
11
BspEI T'CCGG_A
Cuts at: 0 885 3310 4648 7086
Size: 885 2425 1338 2438
Fragments arranged by size:
2438 2425 1338 885
BspHI T'CATG_A
Cuts at: 0 777 7086
Size: 777 6309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4835 5041 7086
Size: 4835 206 2045
Fragments arranged by size:
4835 2045 206
BsrBI CCG'CTC
Cuts at: 0 1272 3161 3711 4138 5085 6429 7086
Size: 1272 1889 550 427 947 1344 657
Fragments arranged by size:
1889 1344 1272 947 657 550 427
BsrDI GCAATG_nn'
Cuts at: 0 132 142 1155 1664 4634 5667 7086
Size: 132 10 1013 509 2970 1033 1419
Fragments arranged by size:
2970 1419 1033 1013 509 132 10
BsrFI r'CCGG_y
Cuts at: 0 1704 3305 3328 4416 5843 6297 6382 6410
Size: 1704 1601 23 1088 1427 454 85 28
Cuts at: 6410 7086
Size: 676
Fragments arranged by size:
1704 1601 1427 1088 676 454 85 28
23
BsrGI T'GTAC_A
Cuts at: 0 1485 7086
Size: 1485 5601
BssHII G'CGCG_C
Cuts at: 0 4056 7086
Size: 4056 3030
BssSI C'ACGA_G
Cuts at: 0 2582 7086
Size: 2582 4504
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1319 2620 6352 7086
Size: 1319 1301 3732 734
Fragments arranged by size:
3732 1319 1301 734
BstBI TT'CG_AA
Cuts at: 0 825 1101 1242 7086
Size: 825 276 141 5844
Fragments arranged by size:
5844 825 276 141
BstEII G'GTnAC_C
Cuts at: 0 1518 2469 3398 3624 7086
Size: 1518 951 929 226 3462
Fragments arranged by size:
3462 1518 951 929 226
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3825 5353 5692 7086
Size: 3825 1528 339 1394
Fragments arranged by size:
3825 1528 1394 339
BstYI r'GATC_y
Cuts at: 0 830 996 1250 1860 2790 3007 3082 7086
Size: 830 166 254 610 930 217 75 4004
Fragments arranged by size:
4004 930 830 610 254 217 166 75
Bsu36I CC'TnA_GG
Cuts at: 0 1879 2465 4655 5125 6461 7086
Size: 1879 586 2190 470 1336 625
Fragments arranged by size:
2190 1879 1336 625 586 470
BtgI C'CryG_G
Cuts at: 0 1526 1721 3270 3879 4391 5207 5309 6437
Size: 1526 195 1549 609 512 816 102 1128
Cuts at: 6437 6569 6836 7086
Size: 132 267 250
Fragments arranged by size:
1549 1526 1128 816 609 512 267 250
195 132 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 414 4087 6014 7086
Size: 414 3673 1927 1072
Fragments arranged by size:
3673 1927 1072 414
BtsI GCAGTG_nn'
Cuts at: 0 3588 4761 5720 6037 7086
Size: 3588 1173 959 317 1049
Fragments arranged by size:
3588 1173 1049 959 317
ClaI AT'CG_AT
Cuts at: 0 1938 4274 7086
Size: 1938 2336 2812
Fragments arranged by size:
2812 2336 1938
DraIII CAC_nnn'GTG
Cuts at: 0 3498 6251 7086
Size: 3498 2753 835
Fragments arranged by size:
3498 2753 835
EaeI y'GGCC_r
Cuts at: 0 1424 3724 4265 5066 5633 6009 6299 6430
Size: 1424 2300 541 801 567 376 290 131
Cuts at: 6430 7086
Size: 656
Fragments arranged by size:
2300 1424 801 656 567 541 376 290
131
EagI C'GGCC_G
Cuts at: 0 6299 7086
Size: 6299 787
EarI CTCTTCn'nnn_
Cuts at: 0 333 2183 2304 2438 2857 3416 3920 4040
Size: 333 1850 121 134 419 559 504 120
Cuts at: 4040 4558 5512 6669 7086
Size: 518 954 1157 417
Fragments arranged by size:
1850 1157 954 559 518 504 419 417
333 134 121 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2979 3315 5609 6337 7086
Size: 2979 336 2294 728 749
Fragments arranged by size:
2979 2294 749 728 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1763 1915 2274 2285 2420 2462 2800 2807
Size: 1763 152 359 11 135 42 338 7
Cuts at: 2807 2881 3640 3832 4045 4107 4135 4354 4630
Size: 74 759 192 213 62 28 219 276
Cuts at: 4630 4849 4852 5114 5517 6121 6130 6341 6362
Size: 219 3 262 403 604 9 211 21
Cuts at: 6362 6588 6712 6829 7086
Size: 226 124 117 257
Fragments arranged by size:
1763 759 604 403 359 338 276 262
257 226 219 219 213 211 192 152
135 124 117 74 62 42 28 21
11 9 7 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1597 4024 7086
Size: 1597 2427 3062
Fragments arranged by size:
3062 2427 1597
EcoO109I rG'GnC_Cy
Cuts at: 0 711 1405 3527 4131 4724 4921 5146 5276
Size: 711 694 2122 604 593 197 225 130
Cuts at: 5276 5372 5748 5818 6026 6148 6478 6906 7086
Size: 96 376 70 208 122 330 428 180
Fragments arranged by size:
2122 711 694 604 593 428 376 330
225 208 197 180 130 122 96 70
EcoRI G'AATT_C
Cuts at: 0 2178 4869 5411 7086
Size: 2178 2691 542 1675
Fragments arranged by size:
2691 2178 1675 542
EcoRV GAT'ATC
Cuts at: 0 1278 7086
Size: 1278 5808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1017 4551 5491 5705 7086
Size: 1017 3534 940 214 1381
Fragments arranged by size:
3534 1381 1017 940 214
FseI GG_CCGG'CC
Cuts at: 0 6386 7086
Size: 6386 700
FspI TGC'GCA
Cuts at: 0 5771 7086
Size: 5771 1315
HaeII r_GCGC'y
Cuts at: 0 1341 3670 4006 5172 5329 5473 7086
Size: 1341 2329 336 1166 157 144 1613
Fragments arranged by size:
2329 1613 1341 1166 336 157 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1787 1819 2023 2055 2312 2344 2780 2812
Size: 1787 32 204 32 257 32 436 32
Cuts at: 2812 3163 3195 3351 3383 3550 3582 4424 4456
Size: 351 32 156 32 167 32 842 32
Cuts at: 4456 5377 5409 7086
Size: 921 32 1677
Fragments arranged by size:
1787 1677 921 842 436 351 257 204
167 156 32 32 32 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 114 5023 5096 7086
Size: 114 4909 73 1990
Fragments arranged by size:
4909 1990 114 73
KasI G'GCGC_C
Cuts at: 0 1337 3666 4002 5325 7086
Size: 1337 2329 336 1323 1761
Fragments arranged by size:
2329 1761 1337 1323 336
KpnI G_GTAC'C
Cuts at: 0 3306 7086
Size: 3306 3780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1731 2318 3169 3394 4058 4410 7086
Size: 1731 587 851 225 664 352 2676
Fragments arranged by size:
2676 1731 851 664 587 352 225
MscI TGG'CCA
Cuts at: 0 1426 4267 5068 6011 7086
Size: 1426 2841 801 943 1075
Fragments arranged by size:
2841 1426 1075 943 801
MslI CAynn'nnrTG
Cuts at: 0 460 1354 1475 2692 2807 5351 7086
Size: 460 894 121 1217 115 2544 1735
Fragments arranged by size:
2544 1735 1217 894 460 121 115
MspA1I CmG'CkG
Cuts at: 0 266 3300 3351 3396 3534 3645 3660 3672
Size: 266 3034 51 45 138 111 15 12
Cuts at: 3672 4011 4351 5407 6163 6181 6439 7086
Size: 339 340 1056 756 18 258 647
Fragments arranged by size:
3034 1056 756 647 340 339 266 258
138 111 51 45 18 15 12
NaeI GCC'GGC
Cuts at: 0 3330 4418 6299 6384 7086
Size: 3330 1088 1881 85 702
Fragments arranged by size:
3330 1881 1088 702 85
NarI GG'CG_CC
Cuts at: 0 1338 3667 4003 5326 7086
Size: 1338 2329 336 1323 1760
Fragments arranged by size:
2329 1760 1338 1323 336
NcoI C'CATG_G
Cuts at: 0 1526 1721 3879 5207 5309 6836 7086
Size: 1526 195 2158 1328 102 1527 250
Fragments arranged by size:
2158 1527 1526 1328 250 195 102
NdeI CA'TA_TG
Cuts at: 0 1920 2057 7086
Size: 1920 137 5029
Fragments arranged by size:
5029 1920 137
NgoMIV G'CCGG_C
Cuts at: 0 3328 4416 6297 6382 7086
Size: 3328 1088 1881 85 704
Fragments arranged by size:
3328 1881 1088 704 85
NheI G'CTAG_C
Cuts at: 0 3024 7086
Size: 3024 4062
NspI r_CATG'y
Cuts at: 0 1317 2768 5195 6690 7086
Size: 1317 1451 2427 1495 396
Fragments arranged by size:
2427 1495 1451 1317 396
PasI CC'CwG_GG
Cuts at: 0 6145 7086
Size: 6145 941
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 1768 2824 5310 7086
Size: 23 1745 1056 2486 1776
Fragments arranged by size:
2486 1776 1745 1056 23
PfoI T'CCnGG_A
Cuts at: 0 1631 3760 4151 6578 7086
Size: 1631 2129 391 2427 508
Fragments arranged by size:
2427 2129 1631 508 391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2241 2273 4825 4857 7086
Size: 2241 32 2552 32 2229
Fragments arranged by size:
2552 2241 2229 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1405 3527 4921 5276 5748 6906 7086
Size: 1405 2122 1394 355 472 1158 180
Fragments arranged by size:
2122 1405 1394 1158 472 355 180
PspOMI G'GGCC_C
Cuts at: 0 4130 5817 6025 6478 7086
Size: 4130 1687 208 453 608
Fragments arranged by size:
4130 1687 608 453 208
PspXI vC'TCGA_Gb
Cuts at: 0 5736 6376 7086
Size: 5736 640 710
Fragments arranged by size:
5736 710 640
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 526 558 7086
Size: 526 32 6528
Fragments arranged by size:
6528 526 32
PstI C_TGCA'G
Cuts at: 0 3677 5495 5727 6648 7086
Size: 3677 1818 232 921 438
Fragments arranged by size:
3677 1818 921 438 232
PvuI CG_AT'CG
Cuts at: 0 4619 7086
Size: 4619 2467
PvuII CAG'CTG
Cuts at: 0 4351 5407 7086
Size: 4351 1056 1679
Fragments arranged by size:
4351 1679 1056
RsrII CG'GwC_CG
Cuts at: 0 3308 7086
Size: 3308 3778
SacII CC_GC'GG
Cuts at: 0 6440 7086
Size: 6440 646
SapI GCTCTTCn'nnn_
Cuts at: 0 4040 4558 7086
Size: 4040 518 2528
Fragments arranged by size:
4040 2528 518
ScaI AGT'ACT
Cuts at: 0 3972 7086
Size: 3972 3114
SfcI C'TryA_G
Cuts at: 0 566 766 3673 5491 5723 6644 7086
Size: 566 200 2907 1818 232 921 442
Fragments arranged by size:
2907 1818 921 566 442 232 200
SfoI GGC'GCC
Cuts at: 0 1339 3668 4004 5327 7086
Size: 1339 2329 336 1323 1759
Fragments arranged by size:
2329 1759 1339 1323 336
SmaI CCC'GGG
Cuts at: 0 3888 5816 7086
Size: 3888 1928 1270
Fragments arranged by size:
3888 1928 1270
SmlI C'TyrA_G
Cuts at: 0 191 683 3538 4300 4489 5106 5736 6376
Size: 191 492 2855 762 189 617 630 640
Cuts at: 6376 7086
Size: 710
Fragments arranged by size:
2855 762 710 640 630 617 492 191
189
SpeI A'CTAG_T
Cuts at: 0 819 7086
Size: 819 6267
SphI G_CATG'C
Cuts at: 0 2768 6690 7086
Size: 2768 3922 396
Fragments arranged by size:
3922 2768 396
SspI AAT'ATT
Cuts at: 0 1465 2266 7086
Size: 1465 801 4820
Fragments arranged by size:
4820 1465 801
StuI AGG'CCT
Cuts at: 0 1261 4495 4660 5123 6640 7081 7086
Size: 1261 3234 165 463 1517 441 5
Fragments arranged by size:
3234 1517 1261 463 441 165 5
StyI C'CwwG_G
Cuts at: 0 1001 1457 1526 1721 3818 3879 4226 4837
Size: 1001 456 69 195 2097 61 347 611
Cuts at: 4837 5207 5309 5564 6130 6671 6836 7086
Size: 370 102 255 566 541 165 250
Fragments arranged by size:
2097 1001 611 566 541 456 370 347
255 250 195 165 102 69 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 327 2027 3297 4465 5297 6158 7086
Size: 327 1700 1270 1168 832 861 928
Fragments arranged by size:
1700 1270 1168 928 861 832 327
TatI w'GTAC_w
Cuts at: 0 1433 1485 3970 7086
Size: 1433 52 2485 3116
Fragments arranged by size:
3116 2485 1433 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 716 748 1691 1723 2804 2836 3586 3618
Size: 716 32 943 32 1081 32 750 32
Cuts at: 3618 3859 3891 4727 4759 7086
Size: 241 32 836 32 2327
Fragments arranged by size:
2327 1081 943 836 750 716 241 32
32 32 32 32 32
XbaI T'CTAG_A
Cuts at: 0 6060 7086
Size: 6060 1026
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3877 5947 6019 6804 7086
Size: 3877 2070 72 785 282
Fragments arranged by size:
3877 2070 785 282 72
XhoI C'TCGA_G
Cuts at: 0 683 5736 6376 7086
Size: 683 5053 640 710
Fragments arranged by size:
5053 710 683 640
XmaI C'CCGG_G
Cuts at: 0 3886 5814 7086
Size: 3886 1928 1272
Fragments arranged by size:
3886 1928 1272
XmnI GAAnn'nnTTC
Cuts at: 0 1679 1991 5471 6454 7086
Size: 1679 312 3480 983 632
Fragments arranged by size:
3480 1679 983 632 312
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PflMI PfoI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SapI ScaI SfcI SfoI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AsiSI AvrII
BaeI BclI BfrBI BsaAI BsiWI BstZ17I CspCI DraI
DrdI EcoICRI FspAI HincII HpaI MfeI MluI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PsiI
SacI SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SnaBI SrfI SwaI Tth111I ZraI