(Circular) (Six-base) MAPSORT of: pFN21AA0284.seq Check: 4882 from: 1 to: 9262 pFN21AA0284 9262 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2143 2546 2932 2143 Size: 403 386 8473 Fragments arranged by size: 8473 403 386 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7072 278 Size: 53 83 186 936 5536 2468 Fragments arranged by size: 5536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6605 1937 Size: 4668 4594 Acc65I G'GTAC_C Cuts at: 6583 6583 Size: 9262 AclI AA'CG_TT Cuts at: 1563 7389 7762 1563 Size: 5826 373 3063 Fragments arranged by size: 5826 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2861 5181 5479 6269 6302 7325 8709 Size: 425 804 2320 298 790 33 1023 1384 Cuts at: 8709 8977 1632 Size: 268 1917 Fragments arranged by size: 2320 1917 1384 1023 804 790 425 298 268 33 AfeI AGC'GCT Cuts at: 3047 3047 Size: 9262 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9065 AflIII A'CryG_T Cuts at: 1203 2076 5565 8161 9138 1203 Size: 873 3489 2596 977 1327 Fragments arranged by size: 3489 2596 1327 977 873 AgeI A'CCGG_T Cuts at: 8093 8093 Size: 9262 AhdI GACnn_n'nnGTC Cuts at: 4642 7991 4642 Size: 3349 5913 AleI CACnn'nnGTG Cuts at: 1166 3533 1166 Size: 2367 6895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3711 1184 Size: 2527 6735 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6098 6130 6098 Size: 32 9230 AlwNI CAG_nnn'CTG Cuts at: 3316 4477 4729 5204 5267 5363 5791 7005 8577 Size: 1161 252 475 63 96 428 1214 1572 Cuts at: 8577 3316 Size: 4001 Fragments arranged by size: 4001 1572 1214 1161 475 428 252 96 63 ApaI G_GGCC'C Cuts at: 3413 3938 4125 4159 4707 5182 5506 5689 3413 Size: 525 187 34 548 475 324 183 6986 Fragments arranged by size: 6986 548 525 475 324 187 183 34 ApaLI G'TGCA_C Cuts at: 7319 8475 7319 Size: 1156 8106 ApoI r'AATT_y Cuts at: 1493 6481 6571 6797 6949 9058 1493 Size: 4988 90 226 152 2109 1697 Fragments arranged by size: 4988 2109 1697 226 152 90 AseI AT'TA_AT Cuts at: 160 1032 7816 160 Size: 872 6784 1606 Fragments arranged by size: 6784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9262 AvaI C'yCGr_G Cuts at: 1870 1943 2513 2686 2761 3169 3350 3817 4070 Size: 73 570 173 75 408 181 467 253 Cuts at: 4070 4160 4488 4621 4777 4905 5067 5351 5509 Size: 90 328 133 156 128 162 284 158 Cuts at: 5509 5650 5682 5697 5928 5954 6003 6587 1870 Size: 141 32 15 231 26 49 584 4545 Fragments arranged by size: 4545 584 570 467 408 328 284 253 231 181 173 162 158 156 141 133 128 90 75 73 49 32 26 15 AvrII C'CTAG_G Cuts at: 4258 4258 Size: 9262 BamHI G'GATC_C Cuts at: 4021 6592 6959 4021 Size: 2571 367 6324 Fragments arranged by size: 6324 2571 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2173 2518 2917 3276 3748 Size: 325 217 627 386 345 399 359 472 Cuts at: 3748 4784 5541 5980 6238 6583 8038 618 Size: 1036 757 439 258 345 1455 1842 Fragments arranged by size: 1842 1455 1036 757 627 472 439 399 386 359 345 345 325 258 217 BanII G_rGCy'C Cuts at: 1391 2364 2409 2463 3329 3413 3758 3938 4112 Size: 973 45 54 866 84 345 180 174 Cuts at: 4112 4125 4159 4707 5182 5250 5506 5558 5677 Size: 13 34 548 475 68 256 52 119 Cuts at: 5677 5689 5861 6431 6581 1391 Size: 12 172 570 150 4072 Fragments arranged by size: 4072 973 866 570 548 475 345 256 180 174 172 150 119 84 68 54 52 45 34 13 12 BbeI G_GCGC'C Cuts at: 2177 3280 5545 2177 Size: 1103 2265 5894 Fragments arranged by size: 5894 2265 1103 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3212 6257 928 Size: 971 1313 3045 3933 Fragments arranged by size: 3933 3045 1313 971 BbvCI CC'TCA_GC Cuts at: 3900 4034 5171 5888 6445 3900 Size: 134 1137 717 557 6717 Fragments arranged by size: 6717 1137 717 557 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2752 2786 4352 4386 7453 Size: 34 50 34 731 34 1566 34 3067 Cuts at: 7453 7487 1903 Size: 34 3678 Fragments arranged by size: 3678 3067 1566 731 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7154 8364 7154 Size: 1210 8052 BclI T'GATC_A Cuts at: 1548 1548 Size: 9262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2900 3408 3725 Size: 107 122 71 631 605 1228 508 317 Cuts at: 3725 3797 3933 4005 4559 4568 4873 5009 7873 Size: 72 136 72 554 9 305 136 2864 Cuts at: 7873 136 Size: 1525 Fragments arranged by size: 2864 1525 1228 631 605 554 508 317 305 136 136 122 107 72 72 71 9 BglII A'GATC_T Cuts at: 9257 9257 Size: 9262 BlpI GC'TnA_GC Cuts at: 2430 4879 5089 5268 5780 6679 2430 Size: 2449 210 179 512 899 5013 Fragments arranged by size: 5013 2449 899 512 210 179 Bme1580I G_kGCm'C Cuts at: 1790 2922 3413 3938 4125 4159 4707 4738 4804 Size: 1132 491 525 187 34 548 31 66 Cuts at: 4804 5182 5506 5521 5689 6243 7323 8479 1790 Size: 378 324 15 168 554 1080 1156 2573 Fragments arranged by size: 2573 1156 1132 1080 554 548 525 491 378 324 187 168 66 34 31 15 BmgBI CAC'GTC Cuts at: 1318 4660 5568 5803 1318 Size: 3342 908 235 4777 Fragments arranged by size: 4777 3342 908 235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3046 3126 3140 Size: 416 53 83 399 24 1629 80 14 Cuts at: 3140 3304 3617 3719 3903 4920 5864 6333 6993 Size: 164 313 102 184 1017 944 469 660 Cuts at: 6993 7951 442 Size: 958 1753 Fragments arranged by size: 1753 1629 1017 958 944 660 469 416 399 313 184 164 102 83 80 53 24 14 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2290 3715 4831 5608 2290 Size: 1425 1116 777 5944 Fragments arranged by size: 5944 1425 1116 777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 3580 4224 4438 4586 5431 5650 7922 Size: 869 1472 644 214 148 845 219 2272 Cuts at: 7922 1239 Size: 2579 Fragments arranged by size: 2579 2272 1472 869 845 644 219 214 148 Bpu10I CC'TnA_GC Cuts at: 3900 4034 5171 5244 5888 6445 3900 Size: 134 1137 73 644 557 6717 Fragments arranged by size: 6717 1137 644 557 134 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2108 3179 7387 8252 8550 2108 Size: 1071 4208 865 298 2820 Fragments arranged by size: 4208 2820 1071 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2248 2483 2720 3506 7925 882 Size: 617 749 235 237 786 4419 2219 Fragments arranged by size: 4419 2219 786 749 617 237 235 BsaAI yAC'GTr Cuts at: 493 1204 4128 7053 493 Size: 711 2924 2925 2702 Fragments arranged by size: 2925 2924 2702 711 BsaBI GATnn'nnATC Cuts at: 2639 6170 9256 2639 Size: 3531 3086 2645 Fragments arranged by size: 3531 3086 2645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2174 2343 3277 4299 Size: 53 83 186 936 641 169 934 1022 Cuts at: 4299 4638 5542 6042 7069 7451 8086 275 Size: 339 904 500 1027 382 635 1451 Fragments arranged by size: 1451 1027 1022 936 934 904 641 635 500 382 339 186 169 83 53 BsaWI w'CCGG_w Cuts at: 2250 3364 3970 7695 8093 8367 8514 8925 2250 Size: 1114 606 3725 398 274 147 411 2587 Fragments arranged by size: 3725 2587 1114 606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4123 4153 4496 4526 4854 Size: 30 165 30 2720 30 343 30 328 Cuts at: 4854 4884 5606 5636 5867 5897 1178 Size: 30 722 30 231 30 4543 Fragments arranged by size: 4543 2720 722 343 328 231 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2197 3485 3554 3967 4028 4568 4946 Size: 943 68 1288 69 413 61 540 378 Cuts at: 4946 5087 5594 5597 5732 5735 5851 5948 6092 Size: 141 507 3 135 3 116 97 144 Cuts at: 6092 1186 Size: 4356 Fragments arranged by size: 4356 1288 943 540 507 413 378 144 141 135 116 97 69 68 61 3 3 BseYI C'CCAG_C Cuts at: 2346 2462 2912 3139 3220 3344 3444 3669 3786 Size: 116 450 227 81 124 100 225 117 Cuts at: 3786 3992 4272 4277 4285 4375 4426 4706 4722 Size: 206 280 5 8 90 51 280 16 Cuts at: 4722 4961 5059 5185 5837 5900 6117 6400 6512 Size: 239 98 126 652 63 217 283 112 Cuts at: 6512 8465 8961 2346 Size: 1953 496 2647 Fragments arranged by size: 2647 1953 652 496 450 283 280 280 239 227 225 217 206 126 124 117 116 112 100 98 90 81 63 51 16 8 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 2411 2603 2756 2771 4752 5768 Size: 573 381 294 192 153 15 1981 1016 Cuts at: 5768 5963 6320 6372 1163 Size: 195 357 52 4053 Fragments arranged by size: 4053 1981 1016 573 381 357 294 195 192 153 52 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2910 3117 3336 6712 7473 8501 Size: 275 186 909 207 219 3376 761 1028 Cuts at: 8501 1540 Size: 2301 Fragments arranged by size: 3376 2301 1028 909 761 275 219 207 186 BsiHKAI G_wGCw'C Cuts at: 2409 3329 3735 6153 7323 7408 8479 2409 Size: 920 406 2418 1170 85 1071 3192 Fragments arranged by size: 3192 2418 1170 1071 920 406 85 BsmI GAATG_Cn' Cuts at: 6773 6866 6773 Size: 93 9169 BsmBI CGTCTCn'nnnn_ Cuts at: 4535 4541 4535 Size: 6 9256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2409 2463 2922 3329 3413 3735 Size: 399 574 45 54 459 407 84 322 Cuts at: 3735 3758 3938 4112 4125 4159 4707 4738 4804 Size: 23 180 174 13 34 548 31 66 Cuts at: 4804 5182 5250 5506 5521 5558 5677 5689 5861 Size: 378 68 256 15 37 119 12 172 Cuts at: 5861 6153 6243 6431 6581 7323 7408 8479 1391 Size: 292 90 188 150 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 574 548 459 407 399 378 322 292 256 188 180 174 172 150 119 90 85 84 68 66 54 45 37 34 31 23 15 13 12 BspEI T'CCGG_A Cuts at: 8925 8925 Size: 9262 BspHI T'CATG_A Cuts at: 7151 8881 7151 Size: 1730 7532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 2546 2826 2932 6363 6617 844 Size: 1299 403 280 106 3431 254 3489 Fragments arranged by size: 3489 3431 1299 403 280 254 106 BsrBI CCG'CTC Cuts at: 1394 1823 2570 3282 3377 4701 5127 5224 5508 Size: 429 747 712 95 1324 426 97 284 Cuts at: 5508 7149 1394 Size: 1641 3507 Fragments arranged by size: 3507 1641 1324 747 712 429 426 284 97 95 BsrDI GCAATG_nn' Cuts at: 65 1248 7757 7931 65 Size: 1183 6509 174 1396 Fragments arranged by size: 6509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2521 3112 3185 4867 5256 5324 5544 Size: 110 711 591 73 1682 389 68 220 Cuts at: 5544 7906 8093 1700 Size: 2362 187 2869 Fragments arranged by size: 2869 2362 1682 711 591 389 220 187 110 73 68 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9262 BssSI C'ACGA_G Cuts at: 2528 2855 5536 7322 8334 2528 Size: 327 2681 1786 1012 3456 Fragments arranged by size: 3456 2681 1786 1012 327 BstAPI GCAn_nnn'nTGC Cuts at: 794 5363 5762 794 Size: 4569 399 4294 Fragments arranged by size: 4569 4294 399 BstBI TT'CG_AA Cuts at: 8066 8066 Size: 9262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9262 BstXI CCAn_nnnn'nTGG Cuts at: 3406 3569 3406 Size: 163 9099 BstYI r'GATC_y Cuts at: 1972 2475 4021 5823 6171 6592 6959 7344 7361 Size: 503 1546 1802 348 421 367 385 17 Cuts at: 7361 8813 8899 8911 9257 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1802 1546 1452 503 421 385 367 348 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2436 4998 6476 2436 Size: 2562 1478 5222 Fragments arranged by size: 5222 2562 1478 BtgI C'CryG_G Cuts at: 513 1065 2410 3100 3391 3405 3435 4858 5250 Size: 552 1345 690 291 14 30 1423 392 Cuts at: 5250 5424 5475 5599 513 Size: 174 51 124 4176 Fragments arranged by size: 4176 1423 1345 690 552 392 291 174 124 51 30 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4053 487 Size: 683 56 2827 5696 Fragments arranged by size: 5696 2827 683 56 BtsI GCAGTG_nn' Cuts at: 2192 5395 5521 6778 7572 7592 2192 Size: 3203 126 1257 794 20 3862 Fragments arranged by size: 3862 3203 1257 794 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3082 3117 529 Size: 35 2518 35 6674 Fragments arranged by size: 6674 2518 35 35 DraI TTT'AAA Cuts at: 6566 6566 Size: 9262 DraIII CAC_nnn'GTG Cuts at: 3713 5833 3713 Size: 2120 7142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2822 8269 809 Size: 507 1506 5447 1802 Fragments arranged by size: 5447 1802 1506 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2736 2892 2907 Size: 54 1425 50 275 18 906 156 15 Cuts at: 2907 3114 3333 3609 3704 4006 4479 4894 5258 Size: 207 219 276 95 302 473 415 364 Cuts at: 5258 5304 5383 5427 5805 6048 6378 6709 7598 Size: 46 79 44 378 243 330 331 889 Cuts at: 7598 9227 8 Size: 1629 43 Fragments arranged by size: 1629 1425 906 889 473 415 378 364 331 330 302 276 275 243 219 207 156 95 79 54 50 46 44 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2907 3114 3333 6709 1537 Size: 275 1095 207 219 3376 4090 Fragments arranged by size: 4090 3376 1095 275 219 207 EarI CTCTTCn'nnn_ Cuts at: 1353 4007 7192 1353 Size: 2654 3185 3423 Fragments arranged by size: 3423 3185 2654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2580 3743 7849 8223 8369 387 Size: 1064 138 991 1163 4106 374 146 1280 Fragments arranged by size: 4106 1280 1163 1064 991 374 146 138 EcoICRI GAG'CTC Cuts at: 2407 3327 2407 Size: 920 8342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2108 2861 3580 4224 4438 4586 Size: 393 425 51 753 719 644 214 148 Cuts at: 4586 5181 5431 5479 5650 6269 6302 7325 7922 Size: 595 250 48 171 619 33 1023 597 Cuts at: 7922 8709 8977 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1023 787 753 719 644 619 597 595 425 393 268 250 214 171 148 51 48 33 EcoNI CCTnn'n_nnAGG Cuts at: 2894 3293 3827 4819 5497 5816 2894 Size: 399 534 992 678 319 6340 Fragments arranged by size: 6340 992 678 534 399 319 EcoO109I rG'GnC_Cy Cuts at: 2384 2884 2971 3079 3410 3739 3867 4067 4121 Size: 500 87 108 331 329 128 200 54 Cuts at: 4121 4155 4156 4255 4474 4774 4902 4911 5229 Size: 34 1 99 219 300 128 9 318 Cuts at: 5229 5480 5502 5686 6205 6454 2384 Size: 251 22 184 519 249 5192 Fragments arranged by size: 5192 519 500 331 329 318 300 251 249 219 200 184 128 128 108 99 87 54 34 22 9 1 EcoRI G'AATT_C Cuts at: 6571 6949 6571 Size: 378 8884 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6193 6193 Size: 9262 FseI GG_CCGG'CC Cuts at: 5260 5260 Size: 9262 FspI TGC'GCA Cuts at: 2009 3881 7768 2009 Size: 1872 3887 3503 Fragments arranged by size: 3887 3503 1872 FspAI rTGC'GCAy Cuts at: 2009 3881 2009 Size: 1872 7390 HaeII r_GCGC'y Cuts at: 1678 2177 2353 2377 3049 3100 3280 3683 4147 Size: 499 176 24 672 51 180 403 464 Cuts at: 4147 4433 4879 5545 5883 8409 1678 Size: 286 446 666 338 2526 2531 Fragments arranged by size: 2531 2526 672 666 499 464 446 403 338 286 180 176 51 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2702 2734 3794 3826 4123 Size: 32 135 32 1130 32 1060 32 297 Cuts at: 4123 4155 4496 4528 4628 4660 4813 4845 5213 Size: 32 341 32 100 32 153 32 368 Cuts at: 5213 5245 5552 5584 6049 6081 7903 7935 7977 Size: 32 307 32 465 32 1822 32 42 Cuts at: 7977 8009 1373 Size: 32 2626 Fragments arranged by size: 2626 1822 1130 1060 465 368 341 307 297 153 135 100 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 6606 6852 669 Size: 988 281 4668 246 3079 Fragments arranged by size: 4668 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9262 HpaI GTT'AAC Cuts at: 6852 6852 Size: 9262 KasI G'GCGC_C Cuts at: 2173 3276 5541 2173 Size: 1103 2265 5894 Fragments arranged by size: 5894 2265 1103 KpnI G_GTAC'C Cuts at: 6587 6587 Size: 9262 MfeI C'AATT_G Cuts at: 6861 6861 Size: 9262 MluI A'CGCG_T Cuts at: 9138 9138 Size: 9262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 3558 3999 4032 5007 5154 5556 8376 Size: 792 1461 441 33 975 147 402 2820 Cuts at: 8376 8560 1305 Size: 184 2007 Fragments arranged by size: 2820 2007 1461 975 792 441 402 184 147 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 2894 4896 5306 5807 6050 Size: 54 1425 343 1062 2002 410 501 243 Cuts at: 6050 6380 10 Size: 330 2892 Fragments arranged by size: 2892 2002 1425 1062 501 410 343 330 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 3533 7220 7579 7738 518 Size: 648 915 1452 3687 359 159 2042 Fragments arranged by size: 3687 2042 1452 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2677 2912 2934 3448 3673 3786 3904 Size: 543 528 235 22 514 225 113 118 Cuts at: 3904 4766 5042 5063 5264 6033 6121 6310 6493 Size: 862 276 21 201 769 88 189 183 Cuts at: 6493 6501 6678 7355 8503 8748 1606 Size: 8 177 677 1148 245 2120 Fragments arranged by size: 2120 1148 862 769 677 543 528 514 276 245 235 225 201 189 183 177 118 113 88 22 21 8 NaeI GCC'GGC Cuts at: 2523 3114 3187 5258 5326 5546 2523 Size: 591 73 2071 68 220 6239 Fragments arranged by size: 6239 2071 591 220 73 68 NarI GG'CG_CC Cuts at: 2174 3277 5542 2174 Size: 1103 2265 5894 Fragments arranged by size: 5894 2265 1103 NcoI C'CATG_G Cuts at: 513 1065 3391 5424 513 Size: 552 2326 2033 4351 Fragments arranged by size: 4351 2326 2033 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9262 NgoMIV G'CCGG_C Cuts at: 2521 3112 3185 5256 5324 5544 2521 Size: 591 73 2071 68 220 6239 Fragments arranged by size: 6239 2071 591 220 73 68 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9262 NotI GC'GGCC_GC Cuts at: 6709 6709 Size: 9262 NspI r_CATG'y Cuts at: 1132 1229 2080 3886 5899 6620 8165 1132 Size: 97 851 1806 2013 721 1545 2229 Fragments arranged by size: 2229 2013 1806 1545 851 721 97 PasI CC'CwG_GG Cuts at: 1348 2786 3761 5233 1348 Size: 1438 975 1472 5377 Fragments arranged by size: 5377 1472 1438 975 PciI A'CATG_T Cuts at: 2076 8161 2076 Size: 6085 3177 PflMI CCAn_nnn'nTGG Cuts at: 1108 3676 4247 4418 5791 5833 1108 Size: 2568 571 171 1373 42 4537 Fragments arranged by size: 4537 2568 1373 571 171 42 PfoI T'CCnGG_A Cuts at: 2013 2109 5447 6519 2013 Size: 96 3338 1072 4756 Fragments arranged by size: 4756 3338 1072 96 PmeI GTTT'AAAC Cuts at: 6566 6566 Size: 9262 PmlI CAC'GTG Cuts at: 4128 4128 Size: 9262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7331 7363 8844 8876 7331 Size: 32 1481 32 7717 Fragments arranged by size: 7717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2384 2884 2971 3079 3867 4474 4774 4911 5229 Size: 500 87 108 788 607 300 137 318 Cuts at: 5229 5480 6205 2384 Size: 251 725 5441 Fragments arranged by size: 5441 788 725 607 500 318 300 251 137 108 87 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9262 PsiI TTA'TAA Cuts at: 6832 6832 Size: 9262 PspOMI G'GGCC_C Cuts at: 3409 3934 4121 4155 4703 5178 5502 5685 3409 Size: 525 187 34 548 475 324 183 6986 Fragments arranged by size: 6986 548 525 475 324 187 183 34 PstI C_TGCA'G Cuts at: 830 2154 2624 2955 3690 6614 830 Size: 1324 470 331 735 2924 3478 Fragments arranged by size: 3478 2924 1324 735 470 331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9262 PvuII CAG'CTG Cuts at: 2149 3448 3904 4766 5063 5264 6033 6121 6310 Size: 1299 456 862 297 201 769 88 189 Cuts at: 6310 6501 2149 Size: 191 4910 Fragments arranged by size: 4910 1299 862 769 456 297 201 191 189 88 SacI G_AGCT'C Cuts at: 2409 3329 2409 Size: 920 8342 SalI G'TCGA_C Cuts at: 1936 6604 1936 Size: 4668 4594 SanDI GG'GwC_CC Cuts at: 2384 2971 3079 3867 4911 5229 5480 2384 Size: 587 108 788 1044 318 251 6166 Fragments arranged by size: 6166 1044 788 587 318 251 108 SbfI CC_TGCA'GG Cuts at: 6614 6614 Size: 9262 ScaI AGT'ACT Cuts at: 7510 9222 7510 Size: 1712 7550 SexAI A'CCwGG_T Cuts at: 3561 3561 Size: 9262 SfcI C'TryA_G Cuts at: 826 1045 2150 2620 2951 3686 4469 6610 7745 Size: 219 1105 470 331 735 783 2141 1135 Cuts at: 7745 8426 8617 826 Size: 681 191 1471 Fragments arranged by size: 2141 1471 1135 1105 783 735 681 470 331 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2900 4559 4568 2900 Size: 1659 9 7594 Fragments arranged by size: 7594 1659 9 SfoI GGC'GCC Cuts at: 2175 3278 5543 2175 Size: 1103 2265 5894 Fragments arranged by size: 5894 2265 1103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9262 SmaI CCC'GGG Cuts at: 1872 2688 3819 4162 4490 4779 5069 5684 5699 Size: 816 1131 343 328 289 290 615 15 Cuts at: 5699 5930 6589 1872 Size: 231 659 4545 Fragments arranged by size: 4545 1131 816 659 615 343 328 290 289 231 15 SmlI C'TyrA_G Cuts at: 820 1017 1943 2123 3194 7366 8267 8529 820 Size: 197 926 180 1071 4172 901 262 1553 Fragments arranged by size: 4172 1553 1071 926 901 262 197 180 SnaBI TAC'GTA Cuts at: 493 7053 493 Size: 6560 2702 SpeI A'CTAG_T Cuts at: 152 5342 152 Size: 5190 4072 SphI G_CATG'C Cuts at: 1132 3886 6620 1132 Size: 2754 2734 3774 Fragments arranged by size: 3774 2754 2734 SspI AAT'ATT Cuts at: 5 52 7186 5 Size: 47 7134 2081 Fragments arranged by size: 7134 2081 47 StuI AGG'CCT Cuts at: 1517 2222 3002 5117 1517 Size: 705 780 2115 5662 Fragments arranged by size: 5662 2115 780 705 StyI C'CwwG_G Cuts at: 513 1065 2379 2730 2895 2922 3084 3391 3453 Size: 552 1314 351 165 27 162 307 62 Cuts at: 3453 4151 4258 5424 6701 513 Size: 698 107 1166 1277 3074 Fragments arranged by size: 3074 1314 1277 1166 698 552 351 307 165 162 107 62 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3530 3701 3766 4538 4848 7289 7306 Size: 1255 1950 171 65 772 310 2441 17 Cuts at: 7306 7459 7644 8089 325 Size: 153 185 445 1498 Fragments arranged by size: 2441 1950 1498 1255 772 445 310 185 171 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 7508 9220 Size: 274 80 33 51 1443 159 5372 1712 Cuts at: 9220 96 Size: 138 Fragments arranged by size: 5372 1712 1443 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 4586 4618 5464 5496 5606 5638 5867 Size: 32 1498 32 846 32 110 32 229 Cuts at: 5867 5899 9222 9254 3056 Size: 32 3323 32 3064 Fragments arranged by size: 3323 3064 1498 846 229 110 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3563 1316 Size: 2247 7015 XbaI T'CTAG_A Cuts at: 6598 6598 Size: 9262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4321 4340 5834 6346 4321 Size: 19 1494 512 7237 Fragments arranged by size: 7237 1494 512 19 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9262 XmaI C'CCGG_G Cuts at: 1870 2686 3817 4160 4488 4777 5067 5682 5697 Size: 816 1131 343 328 289 290 615 15 Cuts at: 5697 5928 6587 1870 Size: 231 659 4545 Fragments arranged by size: 4545 1131 816 659 615 343 328 290 289 231 15 XmnI GAAnn'nnTTC Cuts at: 7391 7391 Size: 9262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7070 276 Size: 53 83 186 936 5536 2468 Fragments arranged by size: 5536 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I EcoRV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SrfI SwaI