(Circular) (Six-base) MAPSORT of: pFN21AA0267.seq Check: 778 from: 1 to: 6712 pFN21AA0267 6712 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4522 278 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4055 1937 Size: 2118 4594 Acc65I G'GTAC_C Cuts at: 4033 4033 Size: 6712 AclI AA'CG_TT Cuts at: 1563 4839 5212 1563 Size: 3276 373 3063 Fragments arranged by size: 3276 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2884 3057 3662 3900 4775 6159 6427 1632 Size: 1252 173 605 238 875 1384 268 1917 Fragments arranged by size: 1917 1384 1252 875 605 268 238 173 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6515 AflIII A'CryG_T Cuts at: 1203 5611 6588 1203 Size: 4408 977 1327 Fragments arranged by size: 4408 1327 977 AgeI A'CCGG_T Cuts at: 5543 5543 Size: 6712 AhdI GACnn_n'nnGTC Cuts at: 5441 5441 Size: 6712 AleI CACnn'nnGTG Cuts at: 1166 3555 1166 Size: 2389 4323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2093 1184 Size: 909 5803 AlwNI CAG_nnn'CTG Cuts at: 2693 2705 4455 6027 2693 Size: 12 1750 1572 3378 Fragments arranged by size: 3378 1750 1572 12 ApaI G_GGCC'C Cuts at: 2293 2293 Size: 6712 ApaLI G'TGCA_C Cuts at: 4769 5925 4769 Size: 1156 5556 ApoI r'AATT_y Cuts at: 1493 3175 3343 3979 4021 4247 4399 6508 1493 Size: 1682 168 636 42 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 636 226 168 152 42 AseI AT'TA_AT Cuts at: 160 1032 5266 160 Size: 872 4234 1606 Fragments arranged by size: 4234 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 AvaI C'yCGr_G Cuts at: 1870 1943 2061 4037 1870 Size: 73 118 1976 4545 Fragments arranged by size: 4545 1976 118 73 BamHI G'GATC_C Cuts at: 4042 4409 4042 Size: 367 6345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2675 3491 3714 4033 Size: 325 217 627 242 646 816 223 319 Cuts at: 4033 5488 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 816 646 627 325 319 242 223 217 BanII G_rGCy'C Cuts at: 1391 2068 2293 4031 1391 Size: 677 225 1738 4072 Fragments arranged by size: 4072 1738 677 225 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5741 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2112 2146 4903 4937 1903 Size: 34 175 34 2757 34 3678 Fragments arranged by size: 3678 2757 175 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4604 5814 4604 Size: 1210 5502 BclI T'GATC_A Cuts at: 1548 3628 1548 Size: 2080 4632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5323 136 Size: 107 122 71 631 605 3651 1525 Fragments arranged by size: 3651 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6707 6707 Size: 6712 BlpI GC'TnA_GC Cuts at: 4129 4129 Size: 6712 Bme1580I G_kGCm'C Cuts at: 1790 2032 2293 4773 5929 1790 Size: 242 261 2480 1156 2573 Fragments arranged by size: 2573 2480 1156 261 242 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6712 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 4443 5401 Size: 416 53 83 399 24 711 2315 958 Cuts at: 5401 442 Size: 1753 Fragments arranged by size: 2315 1753 958 711 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2853 2952 3037 5372 1239 Size: 1614 99 85 2335 2579 Fragments arranged by size: 2579 2335 1614 99 85 Bpu10I CC'TnA_GC Cuts at: 2396 2396 Size: 6712 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4837 5702 6000 4837 Size: 865 298 5549 Fragments arranged by size: 5549 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5375 882 Size: 617 3876 2219 Fragments arranged by size: 3876 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 4503 493 Size: 711 1898 1401 2702 Fragments arranged by size: 2702 1898 1401 711 BsaBI GATnn'nnATC Cuts at: 3771 6706 3771 Size: 2935 3777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2109 4519 4901 Size: 53 83 186 936 513 63 2410 382 Cuts at: 4901 5536 275 Size: 635 1451 Fragments arranged by size: 2410 1451 936 635 513 382 186 83 63 53 BsaWI w'CCGG_w Cuts at: 3609 5145 5543 5817 5964 6375 3609 Size: 1536 398 274 147 411 3946 Fragments arranged by size: 3946 1536 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 2966 2996 3243 Size: 30 165 30 759 30 774 30 247 Cuts at: 3243 3273 1178 Size: 30 4617 Fragments arranged by size: 4617 774 759 247 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2183 2210 2797 1186 Size: 901 96 27 587 5101 Fragments arranged by size: 5101 901 587 96 27 BseYI C'CCAG_C Cuts at: 5915 6411 5915 Size: 496 6216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2429 1163 Size: 573 549 144 5446 Fragments arranged by size: 5446 573 549 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4162 4923 5951 1540 Size: 275 186 2161 761 1028 2301 Fragments arranged by size: 2301 2161 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4773 4858 5929 4773 Size: 85 1071 5556 Fragments arranged by size: 5556 1071 85 BsmI GAATG_Cn' Cuts at: 2361 4223 4316 2361 Size: 1862 93 4757 Fragments arranged by size: 4757 1862 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2068 2293 4031 4773 4858 5929 Size: 399 242 36 225 1738 742 85 1071 Cuts at: 5929 1391 Size: 2174 Fragments arranged by size: 2174 1738 1071 742 399 242 225 85 36 BspEI T'CCGG_A Cuts at: 3609 6375 3609 Size: 2766 3946 BspHI T'CATG_A Cuts at: 4601 6331 4601 Size: 1730 4982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2232 2483 4067 844 Size: 1388 251 1584 3489 Fragments arranged by size: 3489 1584 1388 251 BsrBI CCG'CTC Cuts at: 1394 1823 4599 1394 Size: 429 2776 3507 Fragments arranged by size: 3507 2776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2922 3390 3507 5207 5381 65 Size: 1183 1674 468 117 1700 174 1396 Fragments arranged by size: 1700 1674 1396 1183 468 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 2309 3835 5356 5543 1700 Size: 110 395 104 1526 1521 187 2869 Fragments arranged by size: 2869 1526 1521 395 187 110 104 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6712 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6712 BssSI C'ACGA_G Cuts at: 2723 4772 5784 2723 Size: 2049 1012 3651 Fragments arranged by size: 3651 2049 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2921 3260 3596 794 Size: 2127 339 336 3910 Fragments arranged by size: 3910 2127 339 336 BstBI TT'CG_AA Cuts at: 2524 5516 2524 Size: 2992 3720 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6712 BstXI CCAn_nnnn'nTGG Cuts at: 2831 2831 Size: 6712 BstYI r'GATC_y Cuts at: 1972 2883 2906 4042 4409 4794 4811 6263 6349 Size: 911 23 1136 367 385 17 1452 86 Cuts at: 6349 6361 6707 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1136 911 385 367 346 86 23 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2041 2160 3117 513 Size: 552 939 37 119 957 4108 Fragments arranged by size: 4108 957 939 552 119 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2879 3691 487 Size: 683 56 1653 812 3508 Fragments arranged by size: 3508 1653 812 683 56 BtsI GCAGTG_nn' Cuts at: 2109 3495 4228 5022 5042 2109 Size: 1386 733 794 20 3779 Fragments arranged by size: 3779 1386 794 733 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6677 DraI TTT'AAA Cuts at: 4016 4016 Size: 6712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5719 809 Size: 507 4403 1802 Fragments arranged by size: 4403 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3399 3837 4159 Size: 54 1425 50 275 18 1569 438 322 Cuts at: 4159 5048 6677 8 Size: 889 1629 43 Fragments arranged by size: 1629 1569 1425 889 438 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4159 1537 Size: 275 2347 4090 Fragments arranged by size: 4090 2347 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 3271 4642 1353 Size: 926 992 1371 3423 Fragments arranged by size: 3423 1371 992 926 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 2160 5299 5673 5819 387 Size: 1064 138 438 133 3139 374 146 1280 Fragments arranged by size: 3139 1280 1064 438 374 146 138 133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2853 2884 2952 3037 3057 3662 3900 Size: 393 1221 31 68 85 20 605 238 Cuts at: 3900 4775 5372 6159 6427 1239 Size: 875 597 787 268 1524 Fragments arranged by size: 1524 1221 875 787 605 597 393 268 238 85 68 31 20 EcoRI G'AATT_C Cuts at: 4021 4399 4021 Size: 378 6334 FspI TGC'GCA Cuts at: 5218 5218 Size: 6712 HaeII r_GCGC'y Cuts at: 1678 2220 3852 5859 1678 Size: 542 1632 2007 2531 Fragments arranged by size: 2531 2007 1632 542 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2314 2346 3988 Size: 32 135 32 590 32 120 32 1642 Cuts at: 3988 4020 5353 5385 5427 5459 1373 Size: 32 1333 32 42 32 2626 Fragments arranged by size: 2626 1642 1333 590 135 120 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4056 4302 669 Size: 988 281 2118 246 3079 Fragments arranged by size: 3079 2118 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6712 HpaI GTT'AAC Cuts at: 4302 4302 Size: 6712 KpnI G_GTAC'C Cuts at: 4037 4037 Size: 6712 MfeI C'AATT_G Cuts at: 4311 4311 Size: 6712 MluI A'CGCG_T Cuts at: 6588 6588 Size: 6712 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2444 2967 5826 6010 1305 Size: 1139 523 2859 184 2007 Fragments arranged by size: 2859 2007 1139 523 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3401 10 Size: 54 1425 343 1569 3321 Fragments arranged by size: 3321 1569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2864 3359 3500 3555 4670 5029 5188 Size: 648 1698 495 141 55 1115 359 159 Cuts at: 5188 518 Size: 2042 Fragments arranged by size: 2042 1698 1115 648 495 359 159 141 55 MspA1I CmG'CkG Cuts at: 1606 2831 3663 3673 4128 4805 5953 6198 1606 Size: 1225 832 10 455 677 1148 245 2120 Fragments arranged by size: 2120 1225 1148 832 677 455 245 10 NcoI C'CATG_G Cuts at: 513 1065 2004 2160 513 Size: 552 939 156 5065 Fragments arranged by size: 5065 939 552 156 NdeI CA'TA_TG Cuts at: 387 3808 387 Size: 3421 3291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6712 NotI GC'GGCC_GC Cuts at: 4159 4159 Size: 6712 NspI r_CATG'y Cuts at: 1132 1229 4070 5615 1132 Size: 97 2841 1545 2229 Fragments arranged by size: 2841 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6712 PciI A'CATG_T Cuts at: 5611 5611 Size: 6712 PflMI CCAn_nnn'nTGG Cuts at: 1108 2986 3922 1108 Size: 1878 936 3898 Fragments arranged by size: 3898 1878 936 PfoI T'CCnGG_A Cuts at: 3014 3014 Size: 6712 PmeI GTTT'AAAC Cuts at: 4016 4016 Size: 6712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4781 4813 6294 6326 4781 Size: 32 1481 32 5167 Fragments arranged by size: 5167 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2841 1613 Size: 1228 5484 PsiI TTA'TAA Cuts at: 4282 4282 Size: 6712 PspOMI G'GGCC_C Cuts at: 2289 2289 Size: 6712 PstI C_TGCA'G Cuts at: 830 4064 830 Size: 3234 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6712 PvuII CAG'CTG Cuts at: 2831 2831 Size: 6712 SalI G'TCGA_C Cuts at: 1936 4054 1936 Size: 2118 4594 SapI GCTCTTCn'nnn_ Cuts at: 2279 3271 2279 Size: 992 5720 SbfI CC_TGCA'GG Cuts at: 4064 4064 Size: 6712 ScaI AGT'ACT Cuts at: 4960 6672 4960 Size: 1712 5000 SfcI C'TryA_G Cuts at: 826 1045 4060 5195 5876 6067 826 Size: 219 3015 1135 681 191 1471 Fragments arranged by size: 3015 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6712 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6712 SmaI CCC'GGG Cuts at: 1872 4039 1872 Size: 2167 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4816 5717 5979 820 Size: 197 926 2873 901 262 1553 Fragments arranged by size: 2873 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3102 4503 493 Size: 2609 1401 2702 Fragments arranged by size: 2702 2609 1401 SpeI A'CTAG_T Cuts at: 152 152 Size: 6712 SphI G_CATG'C Cuts at: 1132 4070 1132 Size: 2938 3774 SspI AAT'ATT Cuts at: 5 52 3289 4636 5 Size: 47 3237 1347 2081 Fragments arranged by size: 3237 2081 1347 47 StuI AGG'CCT Cuts at: 1517 2997 1517 Size: 1480 5232 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 2890 3396 3854 3888 4151 Size: 552 939 156 730 506 458 34 263 Cuts at: 4151 513 Size: 3074 Fragments arranged by size: 3074 939 730 552 506 458 263 156 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3545 4739 4756 4909 5094 5539 Size: 1255 1329 636 1194 17 153 185 445 Cuts at: 5539 325 Size: 1498 Fragments arranged by size: 1498 1329 1255 1194 636 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4958 6670 96 Size: 274 80 33 51 1443 2981 1712 138 Fragments arranged by size: 2981 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6672 6704 6672 Size: 32 6680 Tth111I GACn'n_nGTC Cuts at: 1316 4002 1316 Size: 2686 4026 XbaI T'CTAG_A Cuts at: 4048 4048 Size: 6712 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6712 XmaI C'CCGG_G Cuts at: 1870 4037 1870 Size: 2167 4545 XmnI GAAnn'nnTTC Cuts at: 3874 4841 3874 Size: 967 5745 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4520 276 Size: 53 83 186 936 2986 2468 Fragments arranged by size: 2986 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI