Restriction map for
pFN21AA0267
(Linear) (Six-base) MAPSORT of: KIAA0267.seq Check: 6868 from: 1 to: 2007
KIAA0267.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 1052 1657 1895 2007
Size: 879 173 605 238 112
Fragments arranged by size:
879 605 238 173 112
AleI CACnn'nnGTG
Cuts at: 0 1550 2007
Size: 1550 457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 2007
Size: 88 1919
AlwNI CAG_nnn'CTG
Cuts at: 0 688 700 2007
Size: 688 12 1307
Fragments arranged by size:
1307 688 12
ApaI G_GGCC'C
Cuts at: 0 288 2007
Size: 288 1719
ApoI r'AATT_y
Cuts at: 0 1170 1338 1974 2007
Size: 1170 168 636 33
Fragments arranged by size:
1170 636 168 33
AvaI C'yCGr_G
Cuts at: 0 56 2007
Size: 56 1951
BanI G'GyrC_C
Cuts at: 0 24 670 1486 1709 2007
Size: 24 646 816 223 298
Fragments arranged by size:
816 646 298 223 24
BanII G_rGCy'C
Cuts at: 0 63 288 2007
Size: 63 225 1719
Fragments arranged by size:
1719 225 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 107 141 2007
Size: 107 34 1866
Fragments arranged by size:
1866 107 34
BclI T'GATC_A
Cuts at: 0 1623 2007
Size: 1623 384
Bme1580I G_kGCm'C
Cuts at: 0 27 288 2007
Size: 27 261 1719
Fragments arranged by size:
1719 261 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 2007
Size: 123 1884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 947 1032 2007
Size: 848 99 85 975
Fragments arranged by size:
975 848 99 85
Bpu10I CC'TnA_GC
Cuts at: 0 391 2007
Size: 391 1616
BsaAI yAC'GTr
Cuts at: 0 1097 2007
Size: 1097 910
BsaBI GATnn'nnATC
Cuts at: 0 1766 2007
Size: 1766 241
BsaHI Gr'CG_yC
Cuts at: 0 41 104 2007
Size: 41 63 1903
Fragments arranged by size:
1903 63 41
BsaWI w'CCGG_w
Cuts at: 0 1604 2007
Size: 1604 403
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 961 991 1238 1268 2007
Size: 157 30 774 30 247 30 739
Fragments arranged by size:
774 739 247 157 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 178 205 792 2007
Size: 82 96 27 587 1215
Fragments arranged by size:
1215 587 96 82 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 424 2007
Size: 280 144 1583
Fragments arranged by size:
1583 280 144
BsmI GAATG_Cn'
Cuts at: 0 356 2007
Size: 356 1651
Bsp1286I G_dGCh'C
Cuts at: 0 27 63 288 2007
Size: 27 36 225 1719
Fragments arranged by size:
1719 225 36 27
BspEI T'CCGG_A
Cuts at: 0 1604 2007
Size: 1604 403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 478 2007
Size: 227 251 1529
Fragments arranged by size:
1529 251 227
BsrDI GCAATG_nn'
Cuts at: 0 917 1385 1502 2007
Size: 917 468 117 505
Fragments arranged by size:
917 505 468 117
BsrFI r'CCGG_y
Cuts at: 0 200 304 1830 2007
Size: 200 104 1526 177
Fragments arranged by size:
1526 200 177 104
BssSI C'ACGA_G
Cuts at: 0 718 2007
Size: 718 1289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 916 1255 1591 2007
Size: 916 339 336 416
Fragments arranged by size:
916 416 339 336
BstBI TT'CG_AA
Cuts at: 0 519 2007
Size: 519 1488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 826 2007
Size: 826 1181
BstYI r'GATC_y
Cuts at: 0 878 901 2007
Size: 878 23 1106
Fragments arranged by size:
1106 878 23
BtgI C'CryG_G
Cuts at: 0 36 155 1112 2007
Size: 36 119 957 895
Fragments arranged by size:
957 895 119 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 874 1686 2007
Size: 874 812 321
Fragments arranged by size:
874 812 321
BtsI GCAGTG_nn'
Cuts at: 0 104 1490 2007
Size: 104 1386 517
Fragments arranged by size:
1386 517 104
EaeI y'GGCC_r
Cuts at: 0 1394 1832 2007
Size: 1394 438 175
Fragments arranged by size:
1394 438 175
EarI CTCTTCn'nnn_
Cuts at: 0 274 1266 2007
Size: 274 992 741
Fragments arranged by size:
992 741 274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 155 2007
Size: 22 133 1852
Fragments arranged by size:
1852 133 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 879 947 1032 1052 1657 1895 2007
Size: 848 31 68 85 20 605 238 112
Fragments arranged by size:
848 605 238 112 85 68 31 20
HaeII r_GCGC'y
Cuts at: 0 215 1847 2007
Size: 215 1632 160
Fragments arranged by size:
1632 215 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 309 341 1983 2007
Size: 157 32 120 32 1642 24
Fragments arranged by size:
1642 157 120 32 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 962 2007
Size: 439 523 1045
Fragments arranged by size:
1045 523 439
MscI TGG'CCA
Cuts at: 0 1396 2007
Size: 1396 611
MslI CAynn'nnrTG
Cuts at: 0 859 1354 1495 1550 2007
Size: 859 495 141 55 457
Fragments arranged by size:
859 495 457 141 55
MspA1I CmG'CkG
Cuts at: 0 826 1658 1668 2007
Size: 826 832 10 339
Fragments arranged by size:
832 826 339 10
NcoI C'CATG_G
Cuts at: 0 155 2007
Size: 155 1852
NdeI CA'TA_TG
Cuts at: 0 1803 2007
Size: 1803 204
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 1917 2007
Size: 981 936 90
Fragments arranged by size:
981 936 90
PfoI T'CCnGG_A
Cuts at: 0 1009 2007
Size: 1009 998
PshAI GACnn'nnGTC
Cuts at: 0 836 2007
Size: 836 1171
PspOMI G'GGCC_C
Cuts at: 0 284 2007
Size: 284 1723
PvuII CAG'CTG
Cuts at: 0 826 2007
Size: 826 1181
SapI GCTCTTCn'nnn_
Cuts at: 0 274 1266 2007
Size: 274 992 741
Fragments arranged by size:
992 741 274
SnaBI TAC'GTA
Cuts at: 0 1097 2007
Size: 1097 910
SspI AAT'ATT
Cuts at: 0 1284 2007
Size: 1284 723
StuI AGG'CCT
Cuts at: 0 992 2007
Size: 992 1015
StyI C'CwwG_G
Cuts at: 0 155 885 1391 1849 1883 2007
Size: 155 730 506 458 34 124
Fragments arranged by size:
730 506 458 155 124 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 1540 2007
Size: 904 636 467
Fragments arranged by size:
904 636 467
Tth111I GACn'n_nGTC
Cuts at: 0 1997 2007
Size: 1997 10
XmnI GAAnn'nnTTC
Cuts at: 0 1869 2007
Size: 1869 138
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BcgI BclI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI
HaeII Hin4I MmeI MscI MslI MspA1I NcoI NdeI
PflMI PfoI PshAI PspOMI PvuII SapI SnaBI SspI
StuI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspHI BsrBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI SwaI
TatI TstI XbaI XcmI XhoI XmaI ZraI