Restriction map for
pFN21AA0247
(Linear) (Six-base) MAPSORT of: KIAA0247.seq Check: 6165 from: 1 to: 909
KIAA0247.orf
With 182 enzymes: SgfI *
October 24, 2006 15:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 579 667 909
Size: 579 88 242
Fragments arranged by size:
579 242 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 457 909
Size: 245 212 452
Fragments arranged by size:
452 245 212
AflIII A'CryG_T
Cuts at: 0 233 262 909
Size: 233 29 647
Fragments arranged by size:
647 233 29
AleI CACnn'nnGTG
Cuts at: 0 34 909
Size: 34 875
AlwNI CAG_nnn'CTG
Cuts at: 0 745 909
Size: 745 164
ApaI G_GGCC'C
Cuts at: 0 617 909
Size: 617 292
AvaI C'yCGr_G
Cuts at: 0 731 909
Size: 731 178
BanII G_rGCy'C
Cuts at: 0 617 730 909
Size: 617 113 179
Fragments arranged by size:
617 179 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 189 223 909
Size: 189 34 686
Fragments arranged by size:
686 189 34
Bme1580I G_kGCm'C
Cuts at: 0 127 617 909
Size: 127 490 292
Fragments arranged by size:
490 292 127
BmgBI CAC'GTC
Cuts at: 0 263 866 909
Size: 263 603 43
Fragments arranged by size:
603 263 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 611 909
Size: 611 298
Bpu10I CC'TnA_GC
Cuts at: 0 789 909
Size: 789 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 176 492 909
Size: 176 316 417
Fragments arranged by size:
417 316 176
BsaWI w'CCGG_w
Cuts at: 0 135 909
Size: 135 774
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 472 497 502 527 909
Size: 472 25 5 25 382
Fragments arranged by size:
472 382 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 391 547 639 660 886 909
Size: 388 3 156 92 21 226 23
Fragments arranged by size:
388 226 156 92 23 21 3
BsiHKAI G_wGCw'C
Cuts at: 0 31 909
Size: 31 878
BsmBI CGTCTCn'nnnn_
Cuts at: 0 98 313 909
Size: 98 215 596
Fragments arranged by size:
596 215 98
Bsp1286I G_dGCh'C
Cuts at: 0 31 127 617 730 909
Size: 31 96 490 113 179
Fragments arranged by size:
490 179 113 96 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 667 909
Size: 579 88 242
Fragments arranged by size:
579 242 88
BsrFI r'CCGG_y
Cuts at: 0 784 909
Size: 784 125
BssSI C'ACGA_G
Cuts at: 0 403 909
Size: 403 506
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 909
Size: 648 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 498 909
Size: 384 114 411
Fragments arranged by size:
411 384 114
BtgI C'CryG_G
Cuts at: 0 6 44 53 290 383 909
Size: 6 38 9 237 93 526
Fragments arranged by size:
526 237 93 38 9 6
BtsI GCAGTG_nn'
Cuts at: 0 207 909
Size: 207 702
DraIII CAC_nnn'GTG
Cuts at: 0 564 909
Size: 564 345
EaeI y'GGCC_r
Cuts at: 0 782 909
Size: 782 127
EarI CTCTTCn'nnn_
Cuts at: 0 777 909
Size: 777 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 457 909
Size: 245 212 452
Fragments arranged by size:
452 245 212
EcoO109I rG'GnC_Cy
Cuts at: 0 345 662 693 909
Size: 345 317 31 216
Fragments arranged by size:
345 317 216 31
HaeII r_GCGC'y
Cuts at: 0 391 909
Size: 391 518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 741 773 909
Size: 472 32 237 32 136
Fragments arranged by size:
472 237 136 32 32
MslI CAynn'nnrTG
Cuts at: 0 34 496 909
Size: 34 462 413
Fragments arranged by size:
462 413 34
MspA1I CmG'CkG
Cuts at: 0 648 909
Size: 648 261
NaeI GCC'GGC
Cuts at: 0 786 909
Size: 786 123
NcoI C'CATG_G
Cuts at: 0 6 290 909
Size: 6 284 619
Fragments arranged by size:
619 284 6
NgoMIV G'CCGG_C
Cuts at: 0 784 909
Size: 784 125
NspI r_CATG'y
Cuts at: 0 237 909
Size: 237 672
PciI A'CATG_T
Cuts at: 0 233 909
Size: 233 676
PflMI CCAn_nnn'nTGG
Cuts at: 0 148 909
Size: 148 761
PpuMI rG'GwC_Cy
Cuts at: 0 345 909
Size: 345 564
PspOMI G'GGCC_C
Cuts at: 0 613 909
Size: 613 296
PstI C_TGCA'G
Cuts at: 0 181 428 678 825 909
Size: 181 247 250 147 84
Fragments arranged by size:
250 247 181 147 84
PvuII CAG'CTG
Cuts at: 0 648 909
Size: 648 261
SanDI GG'GwC_CC
Cuts at: 0 345 909
Size: 345 564
SexAI A'CCwGG_T
Cuts at: 0 482 909
Size: 482 427
SfcI C'TryA_G
Cuts at: 0 177 359 424 674 821 909
Size: 177 182 65 250 147 88
Fragments arranged by size:
250 182 177 147 88 65
StyI C'CwwG_G
Cuts at: 0 6 290 634 657 909
Size: 6 284 344 23 252
Fragments arranged by size:
344 284 252 23 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 343 768 909
Size: 343 425 141
Fragments arranged by size:
425 343 141
TatI w'GTAC_w
Cuts at: 0 585 875 909
Size: 585 290 34
Fragments arranged by size:
585 290 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 329 361 909
Size: 50 32 247 32 548
Fragments arranged by size:
548 247 50 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 484 909
Size: 484 425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 337 472 909
Size: 337 135 437
Fragments arranged by size:
437 337 135
XmnI GAAnn'nnTTC
Cuts at: 0 442 909
Size: 442 467
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI AvaI BanII
BcgI Bme1580I BmgBI BmrI Bpu10I BsaI BsaWI BsaXI
BseRI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI
BstXI BtgI BtsI DraIII EaeI EarI Eco57MI EcoO109I
HaeII Hin4I MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PflMI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpmI BpuEI
BsaAI BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmaI ZraI