(Circular) (Six-base) MAPSORT of: pFN21AA0234.seq Check: 1036 from: 1 to: 9151 pFN21AA0234 9151 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4555 4848 5359 4555 Size: 293 511 8347 Fragments arranged by size: 8347 511 293 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 6961 278 Size: 53 83 186 936 2305 3120 2468 Fragments arranged by size: 3120 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2464 3841 5772 6494 1937 Size: 527 1377 1931 722 4594 Fragments arranged by size: 4594 1931 1377 722 527 Acc65I G'GTAC_C Cuts at: 6472 6472 Size: 9151 AclI AA'CG_TT Cuts at: 1563 6192 7278 7651 1563 Size: 4629 1086 373 3063 Fragments arranged by size: 4629 3063 1086 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2964 3660 4112 4223 4268 4541 4563 5612 Size: 1332 696 452 111 45 273 22 1049 Cuts at: 5612 5709 5763 6203 6372 7214 8598 8866 1632 Size: 97 54 440 169 842 1384 268 1917 Fragments arranged by size: 1917 1384 1332 1049 842 696 452 440 273 268 169 111 97 54 45 22 AfeI AGC'GCT Cuts at: 4739 4739 Size: 9151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8954 AflIII A'CryG_T Cuts at: 1203 2673 8050 9027 1203 Size: 1470 5377 977 1327 Fragments arranged by size: 5377 1470 1327 977 AgeI A'CCGG_T Cuts at: 7982 7982 Size: 9151 AhdI GACnn_n'nnGTC Cuts at: 7880 7880 Size: 9151 AleI CACnn'nnGTG Cuts at: 1166 2041 2754 1166 Size: 875 713 7563 Fragments arranged by size: 7563 875 713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3314 5682 1184 Size: 2130 2368 4653 Fragments arranged by size: 4653 2368 2130 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4341 4373 4341 Size: 32 9119 AlwNI CAG_nnn'CTG Cuts at: 3797 4246 4525 4606 5117 5228 5596 5786 6042 Size: 449 279 81 511 111 368 190 256 Cuts at: 6042 6052 6894 8466 3797 Size: 10 842 1572 4482 Fragments arranged by size: 4482 1572 842 511 449 368 279 256 190 111 81 10 ApaLI G'TGCA_C Cuts at: 3800 7208 8364 3800 Size: 3408 1156 4587 Fragments arranged by size: 4587 3408 1156 ApoI r'AATT_y Cuts at: 1493 2072 2276 2286 3074 3146 5071 5516 6084 Size: 579 204 10 788 72 1925 445 568 Cuts at: 6084 6110 6460 6686 6838 8947 1493 Size: 26 350 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 788 579 568 445 350 226 204 152 72 26 10 AseI AT'TA_AT Cuts at: 160 1032 7705 160 Size: 872 6673 1606 Fragments arranged by size: 6673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 AvaI C'yCGr_G Cuts at: 1870 1943 4701 6476 1870 Size: 73 2758 1775 4545 Fragments arranged by size: 4545 2758 1775 73 BamHI G'GATC_C Cuts at: 6481 6848 6481 Size: 367 8784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2904 3049 3298 4537 4664 Size: 325 217 627 1117 145 249 1239 127 Cuts at: 4664 5320 5371 6472 7927 618 Size: 656 51 1101 1455 1842 Fragments arranged by size: 1842 1455 1239 1117 1101 656 627 325 249 217 145 127 51 BanII G_rGCy'C Cuts at: 1391 2715 3744 4071 4631 5900 5977 5991 6148 Size: 1324 1029 327 560 1269 77 14 157 Cuts at: 6148 6235 6242 6470 1391 Size: 87 7 228 4072 Fragments arranged by size: 4072 1324 1269 1029 560 327 228 157 87 77 14 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3404 3718 928 Size: 971 1212 293 314 6361 Fragments arranged by size: 6361 1212 971 314 293 BbvCI CC'TCA_GC Cuts at: 4449 4449 Size: 9151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5270 5304 7342 7376 1903 Size: 34 3333 34 2038 34 3678 Fragments arranged by size: 3678 3333 2038 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5285 7043 8253 5285 Size: 1758 1210 6183 Fragments arranged by size: 6183 1758 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9151 BfrBI ATG'CAT Cuts at: 4396 4396 Size: 9151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3981 4984 5605 Size: 107 122 71 631 605 2309 1003 621 Cuts at: 5605 5675 7762 136 Size: 70 2087 1525 Fragments arranged by size: 2309 2087 1525 1003 631 621 605 122 107 71 70 BglII A'GATC_T Cuts at: 2328 9146 2328 Size: 6818 2333 BlpI GC'TnA_GC Cuts at: 3879 4707 5721 6568 3879 Size: 828 1014 847 6462 Fragments arranged by size: 6462 1014 847 828 Bme1580I G_kGCm'C Cuts at: 1790 2048 2761 2909 3303 3804 4192 5445 7212 Size: 258 713 148 394 501 388 1253 1767 Cuts at: 7212 8368 1790 Size: 1156 2573 Fragments arranged by size: 2573 1767 1253 1156 713 501 394 388 258 148 BmgBI CAC'GTC Cuts at: 1318 3109 1318 Size: 1791 7360 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 5304 5463 Size: 416 53 83 399 24 1338 2549 159 Cuts at: 5463 6882 7840 442 Size: 1419 958 1753 Fragments arranged by size: 2549 1753 1419 1338 958 416 399 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5495 1056 Size: 4439 4712 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5065 5782 6162 5065 Size: 717 380 8054 Fragments arranged by size: 8054 717 380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2920 3100 3379 4214 4663 4787 5056 Size: 1010 671 180 279 835 449 124 269 Cuts at: 5056 5867 5871 5927 6002 6054 6119 6170 7811 Size: 811 4 56 75 52 65 51 1641 Cuts at: 7811 1239 Size: 2579 Fragments arranged by size: 2579 1641 1010 835 811 671 449 279 269 180 124 75 65 56 52 51 4 Bpu10I CC'TnA_GC Cuts at: 2589 3465 4184 4449 6241 2589 Size: 876 719 265 1792 5499 Fragments arranged by size: 5499 1792 876 719 265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2675 2722 4269 4385 5048 5111 6032 7276 8141 Size: 47 1547 116 663 63 921 1244 865 Cuts at: 8141 8439 2675 Size: 298 3387 Fragments arranged by size: 3387 1547 1244 921 865 663 298 116 63 47 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5211 7814 882 Size: 617 3712 2603 2219 Fragments arranged by size: 3712 2603 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3313 3942 6942 493 Size: 711 2109 629 3000 2702 Fragments arranged by size: 3000 2702 2109 711 629 BsaBI GATnn'nnATC Cuts at: 3783 9145 3783 Size: 5362 3789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 3838 3867 6958 Size: 53 83 186 936 2149 156 29 3091 Cuts at: 6958 7340 7975 275 Size: 382 635 1451 Fragments arranged by size: 3091 2149 1451 936 635 382 186 156 83 53 29 BsaWI w'CCGG_w Cuts at: 2038 4044 7584 7982 8256 8403 8814 2038 Size: 2006 3540 398 274 147 411 2375 Fragments arranged by size: 3540 2375 2006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3191 3221 4026 4056 5467 Size: 30 165 30 1788 30 805 30 1411 Cuts at: 5467 5497 5847 5877 5900 5930 6206 6236 1178 Size: 30 350 30 23 30 276 30 4093 Fragments arranged by size: 4093 1788 1411 805 350 276 165 30 30 30 30 30 30 30 30 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2193 2287 2519 3212 3455 3752 3845 4228 Size: 1007 94 232 693 243 297 93 383 Cuts at: 4228 5441 5774 5999 1186 Size: 1213 333 225 4338 Fragments arranged by size: 4338 1213 1007 693 383 333 297 243 232 225 94 93 BseYI C'CCAG_C Cuts at: 2064 4525 4740 5161 5191 5378 8354 8850 2064 Size: 2461 215 421 30 187 2976 496 2365 Fragments arranged by size: 2976 2461 2365 496 421 215 187 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 2701 3591 5130 5187 1163 Size: 573 720 245 890 1539 57 5127 Fragments arranged by size: 5127 1539 890 720 573 245 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6601 7362 8390 1540 Size: 275 186 4600 761 1028 2301 Fragments arranged by size: 4600 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3744 3804 4025 4071 5991 6242 7212 7297 8368 Size: 60 221 46 1920 251 970 85 1071 Cuts at: 8368 3744 Size: 4527 Fragments arranged by size: 4527 1920 1071 970 251 221 85 60 46 BsmI GAATG_Cn' Cuts at: 6662 6755 6662 Size: 93 9058 BsmBI CGTCTCn'nnnn_ Cuts at: 3764 3764 Size: 9151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2715 2761 2909 3303 3744 3804 Size: 399 258 667 46 148 394 441 60 Cuts at: 3804 4025 4071 4192 4631 5445 5900 5977 5991 Size: 221 46 121 439 814 455 77 14 Cuts at: 5991 6148 6235 6242 6470 7212 7297 8368 1391 Size: 157 87 7 228 742 85 1071 2174 Fragments arranged by size: 2174 1071 814 742 667 455 441 439 399 394 258 228 221 157 148 121 87 85 77 60 46 46 14 7 BspEI T'CCGG_A Cuts at: 8814 8814 Size: 9151 BspHI T'CATG_A Cuts at: 3513 5991 7040 8770 3513 Size: 2478 1049 1730 3894 Fragments arranged by size: 3894 2478 1730 1049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3682 4555 4848 4983 5005 5359 6015 6506 Size: 2838 873 293 135 22 354 656 491 Cuts at: 6506 844 Size: 3489 Fragments arranged by size: 3489 2838 873 656 491 354 293 135 22 BsrBI CCG'CTC Cuts at: 1394 1823 2323 7038 1394 Size: 429 500 4715 3507 Fragments arranged by size: 4715 3507 500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2094 2363 2684 4586 4728 7646 7820 Size: 1183 846 269 321 1902 142 2918 174 Cuts at: 7820 65 Size: 1396 Fragments arranged by size: 2918 1902 1396 1183 846 321 269 174 142 BsrFI r'CCGG_y Cuts at: 1700 1810 3705 5142 7795 7982 1700 Size: 110 1895 1437 2653 187 2869 Fragments arranged by size: 2869 2653 1895 1437 187 110 BsrGI T'GTAC_A Cuts at: 96 3040 96 Size: 2944 6207 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9151 BssSI C'ACGA_G Cuts at: 3597 3734 4206 4455 7211 8223 3597 Size: 137 472 249 2756 1012 4525 Fragments arranged by size: 4525 2756 1012 472 249 137 BstAPI GCAn_nnn'nTGC Cuts at: 794 3930 5596 6052 794 Size: 3136 1666 456 3893 Fragments arranged by size: 3893 3136 1666 456 BstBI TT'CG_AA Cuts at: 2740 7955 2740 Size: 5215 3936 BstEII G'GTnAC_C Cuts at: 1184 4919 6016 1184 Size: 3735 1097 4319 Fragments arranged by size: 4319 3735 1097 BstXI CCAn_nnnn'nTGG Cuts at: 2569 5317 2569 Size: 2748 6403 BstYI r'GATC_y Cuts at: 1972 2328 3778 4908 6481 6848 7233 7250 8702 Size: 356 1450 1130 1573 367 385 17 1452 Cuts at: 8702 8788 8800 9146 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1573 1452 1450 1130 385 367 356 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3195 5613 5961 3195 Size: 2418 348 6385 Fragments arranged by size: 6385 2418 348 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2797 3538 5422 513 Size: 552 939 147 646 741 1884 4242 Fragments arranged by size: 4242 1884 939 741 646 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8412 Fragments arranged by size: 8412 683 56 BtsI GCAGTG_nn' Cuts at: 2761 3160 4472 5084 5402 5435 5486 5784 5818 Size: 399 1312 612 318 33 51 298 34 Cuts at: 5818 6667 7461 7481 2761 Size: 849 794 20 4431 Fragments arranged by size: 4431 1312 849 794 612 399 318 298 51 34 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9116 DraI TTT'AAA Cuts at: 2304 6455 2304 Size: 4151 5000 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8158 809 Size: 507 6842 1802 Fragments arranged by size: 6842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4159 4314 4467 Size: 54 1425 50 275 18 2329 155 153 Cuts at: 4467 5549 6598 7487 9116 8 Size: 1082 1049 889 1629 43 Fragments arranged by size: 2329 1629 1425 1082 1049 889 275 155 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6598 1537 Size: 275 4786 4090 Fragments arranged by size: 4786 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4173 4986 5344 6391 7081 1353 Size: 2820 813 358 1047 690 3423 Fragments arranged by size: 3423 2820 1047 813 690 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 2939 7738 8112 8258 387 Size: 1064 138 543 807 4799 374 146 1280 Fragments arranged by size: 4799 1280 1064 807 543 374 146 138 EcoICRI GAG'CTC Cuts at: 3742 4069 5989 6240 3742 Size: 327 1920 251 6653 Fragments arranged by size: 6653 1920 327 251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2920 2964 3100 3379 3660 4112 Size: 393 617 671 44 136 279 281 452 Cuts at: 4112 4214 4223 4268 4541 4563 4663 4787 5056 Size: 102 9 45 273 22 100 124 269 Cuts at: 5056 5612 5709 5763 5867 5871 5927 6002 6054 Size: 556 97 54 104 4 56 75 52 Cuts at: 6054 6119 6170 6203 6372 7214 7811 8598 8866 Size: 65 51 33 169 842 597 787 268 Cuts at: 8866 1239 Size: 1524 Fragments arranged by size: 1524 842 787 671 617 597 556 452 393 281 279 273 269 268 169 136 124 104 102 100 97 75 65 56 54 52 51 45 44 33 22 9 4 EcoNI CCTnn'n_nnAGG Cuts at: 4262 4427 5408 6011 4262 Size: 165 981 603 7402 Fragments arranged by size: 7402 981 603 165 EcoO109I rG'GnC_Cy Cuts at: 2558 3850 4357 4603 4951 4972 5209 5623 6004 Size: 1292 507 246 348 21 237 414 381 Cuts at: 6004 2558 Size: 5705 Fragments arranged by size: 5705 1292 507 414 381 348 246 237 21 EcoRI G'AATT_C Cuts at: 2072 3074 5071 6084 6460 6838 2072 Size: 1002 1997 1013 376 378 4385 Fragments arranged by size: 4385 1997 1013 1002 378 376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2102 2716 2102 Size: 614 8537 FspI TGC'GCA Cuts at: 7657 7657 Size: 9151 HaeII r_GCGC'y Cuts at: 1678 3718 4741 5162 8298 1678 Size: 2040 1023 421 3136 2531 Fragments arranged by size: 3136 2531 2040 1023 421 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3763 3795 4026 4058 4312 Size: 32 135 32 2191 32 231 32 254 Cuts at: 4312 4344 5105 5137 5210 5242 5900 5932 6206 Size: 32 761 32 73 32 658 32 274 Cuts at: 6206 6238 6270 6368 6400 7792 7824 7866 7898 Size: 32 32 98 32 1392 32 42 32 Cuts at: 7898 1373 Size: 2626 Fragments arranged by size: 2626 2191 1392 761 658 274 254 231 135 98 73 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 2465 3649 5937 6495 6741 Size: 988 281 247 280 1184 2288 558 246 Cuts at: 6741 669 Size: 3079 Fragments arranged by size: 3079 2288 1184 988 558 281 280 247 246 HindIII A'AGCT_T Cuts at: 748 2816 2946 4884 748 Size: 2068 130 1938 5015 Fragments arranged by size: 5015 2068 1938 130 HpaI GTT'AAC Cuts at: 6741 6741 Size: 9151 KpnI G_GTAC'C Cuts at: 6476 6476 Size: 9151 MfeI C'AATT_G Cuts at: 6750 6750 Size: 9151 MluI A'CGCG_T Cuts at: 9027 9027 Size: 9151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3045 3203 3756 4488 5369 5931 6110 8265 Size: 1740 158 553 732 881 562 179 2155 Cuts at: 8265 8449 1305 Size: 184 2007 Fragments arranged by size: 2155 2007 1740 881 732 562 553 184 179 158 MscI TGG'CCA Cuts at: 10 64 1489 1832 4316 4469 10 Size: 54 1425 343 2484 153 4692 Fragments arranged by size: 4692 2484 1425 343 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2041 2754 3273 3296 3387 3512 3530 Size: 648 875 713 519 23 91 125 18 Cuts at: 3530 4005 7109 7468 7627 518 Size: 475 3104 359 159 2042 Fragments arranged by size: 3104 2042 875 713 648 519 475 359 159 125 91 23 18 MspA1I CmG'CkG Cuts at: 1606 2153 4336 4525 5284 5378 5716 6049 6392 Size: 547 2183 189 759 94 338 333 343 Cuts at: 6392 6567 7244 8392 8637 1606 Size: 175 677 1148 245 2120 Fragments arranged by size: 2183 2120 1148 759 677 547 343 338 333 245 189 175 94 NaeI GCC'GGC Cuts at: 3707 3707 Size: 9151 NcoI C'CATG_G Cuts at: 513 1065 2004 3538 5422 513 Size: 552 939 1534 1884 4242 Fragments arranged by size: 4242 1884 1534 939 552 NdeI CA'TA_TG Cuts at: 387 3045 387 Size: 2658 6493 NgoMIV G'CCGG_C Cuts at: 3705 3705 Size: 9151 NheI G'CTAG_C Cuts at: 1052 5491 1052 Size: 4439 4712 NotI GC'GGCC_GC Cuts at: 6598 6598 Size: 9151 NsiI A_TGCA'T Cuts at: 4398 4398 Size: 9151 NspI r_CATG'y Cuts at: 1132 1229 2628 3037 6509 8054 1132 Size: 97 1399 409 3472 1545 2229 Fragments arranged by size: 3472 2229 1545 1399 409 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9151 PciI A'CATG_T Cuts at: 8050 8050 Size: 9151 PflMI CCAn_nnn'nTGG Cuts at: 1108 3222 5655 1108 Size: 2114 2433 4604 Fragments arranged by size: 4604 2433 2114 PfoI T'CCnGG_A Cuts at: 4422 5033 5887 4422 Size: 611 854 7686 Fragments arranged by size: 7686 854 611 PmeI GTTT'AAAC Cuts at: 6455 6455 Size: 9151 PmlI CAC'GTG Cuts at: 3942 3942 Size: 9151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7220 7252 8733 8765 7220 Size: 32 1481 32 7606 Fragments arranged by size: 7606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2558 4357 4972 5209 6004 2558 Size: 1799 615 237 795 5705 Fragments arranged by size: 5705 1799 795 615 237 PshAI GACnn'nnGTC Cuts at: 1613 5406 1613 Size: 3793 5358 PsiI TTA'TAA Cuts at: 6721 6721 Size: 9151 PstI C_TGCA'G Cuts at: 830 5016 5283 5397 5592 6057 6503 830 Size: 4186 267 114 195 465 446 3478 Fragments arranged by size: 4186 3478 465 446 267 195 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9151 PvuII CAG'CTG Cuts at: 4336 4525 5284 5378 5716 6049 6392 4336 Size: 189 759 94 338 333 343 7095 Fragments arranged by size: 7095 759 343 338 333 189 94 SacI G_AGCT'C Cuts at: 3744 4071 5991 6242 3744 Size: 327 1920 251 6653 Fragments arranged by size: 6653 1920 327 251 SacII CC_GC'GG Cuts at: 2154 2154 Size: 9151 SalI G'TCGA_C Cuts at: 1936 2463 6493 1936 Size: 527 4030 4594 Fragments arranged by size: 4594 4030 527 SapI GCTCTTCn'nnn_ Cuts at: 4173 4986 6391 4173 Size: 813 1405 6933 Fragments arranged by size: 6933 1405 813 SbfI CC_TGCA'GG Cuts at: 6503 6503 Size: 9151 ScaI AGT'ACT Cuts at: 7399 9111 7399 Size: 1712 7439 SexAI A'CCwGG_T Cuts at: 3401 3401 Size: 9151 SfcI C'TryA_G Cuts at: 826 1045 2341 2458 2480 2499 3054 5012 5279 Size: 219 1296 117 22 19 555 1958 267 Cuts at: 5279 5393 5588 5738 6053 6499 7634 8315 8506 Size: 114 195 150 315 446 1135 681 191 Cuts at: 8506 826 Size: 1471 Fragments arranged by size: 1958 1471 1296 1135 681 555 446 315 267 219 195 191 150 117 114 22 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9151 SmaI CCC'GGG Cuts at: 1872 4703 6478 1872 Size: 2831 1775 4545 Fragments arranged by size: 4545 2831 1775 SmlI C'TyrA_G Cuts at: 820 1017 1943 2690 2701 4284 4364 5063 5090 Size: 197 926 747 11 1583 80 699 27 Cuts at: 5090 6011 7255 8156 8418 820 Size: 921 1244 901 262 1553 Fragments arranged by size: 1583 1553 1244 926 921 901 747 699 262 197 80 27 11 SnaBI TAC'GTA Cuts at: 493 6942 493 Size: 6449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9151 SphI G_CATG'C Cuts at: 1132 6509 1132 Size: 5377 3774 SrfI GCCC'GGGC Cuts at: 4703 4703 Size: 9151 SspI AAT'ATT Cuts at: 5 52 5824 7075 5 Size: 47 5772 1251 2081 Fragments arranged by size: 5772 2081 1251 47 StuI AGG'CCT Cuts at: 1517 4439 5048 5765 1517 Size: 2922 609 717 4903 Fragments arranged by size: 4903 2922 717 609 StyI C'CwwG_G Cuts at: 513 1065 2004 3538 3622 4059 4143 5422 5836 Size: 552 939 1534 84 437 84 1279 414 Cuts at: 5836 6590 513 Size: 754 3074 Fragments arranged by size: 3074 1534 1279 939 754 552 437 414 84 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4652 7178 7195 7348 7533 7978 Size: 1255 2937 135 2526 17 153 185 445 Cuts at: 7978 325 Size: 1498 Fragments arranged by size: 2937 2526 1498 1255 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2976 3040 3656 Size: 274 80 33 51 1443 999 64 616 Cuts at: 3656 7397 9109 96 Size: 3741 1712 138 Fragments arranged by size: 3741 1712 1443 999 616 274 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5467 5499 5847 5879 9111 9143 2000 Size: 32 3435 32 348 32 3232 32 2008 Fragments arranged by size: 3435 3232 2008 348 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9151 XbaI T'CTAG_A Cuts at: 4936 6487 4936 Size: 1551 7600 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2084 2154 4687 5493 5677 2084 Size: 70 2533 806 184 5558 Fragments arranged by size: 5558 2533 806 184 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9151 XmaI C'CCGG_G Cuts at: 1870 4701 6476 1870 Size: 2831 1775 4545 Fragments arranged by size: 4545 2831 1775 XmnI GAAnn'nnTTC Cuts at: 3074 7280 3074 Size: 4206 4945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 6959 276 Size: 53 83 186 936 2305 3120 2468 Fragments arranged by size: 3120 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SwaI