(Circular) (Six-base) MAPSORT of: pFN21AA0231.seq Check: 3456 from: 1 to: 7114 pFN21AA0231 7114 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2082 4924 278 Size: 53 83 186 936 546 2842 2468 Fragments arranged by size: 2842 2468 936 546 186 83 53 AccI GT'mk_AC Cuts at: 1937 3435 4457 1937 Size: 1498 1022 4594 Fragments arranged by size: 4594 1498 1022 Acc65I G'GTAC_C Cuts at: 4435 4435 Size: 7114 AclI AA'CG_TT Cuts at: 1563 4387 5241 5614 1563 Size: 2824 854 373 3063 Fragments arranged by size: 3063 2824 854 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3050 3884 5177 6561 6829 1632 Size: 1418 834 1293 1384 268 1917 Fragments arranged by size: 1917 1418 1384 1293 834 268 AfeI AGC'GCT Cuts at: 2906 2906 Size: 7114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6917 AflIII A'CryG_T Cuts at: 1203 6013 6990 1203 Size: 4810 977 1327 Fragments arranged by size: 4810 1327 977 AgeI A'CCGG_T Cuts at: 5945 5945 Size: 7114 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4140 4199 1184 Size: 2956 59 4099 Fragments arranged by size: 4099 2956 59 AlwNI CAG_nnn'CTG Cuts at: 2525 3700 4105 4857 6429 2525 Size: 1175 405 752 1572 3210 Fragments arranged by size: 3210 1572 1175 752 405 ApaLI G'TGCA_C Cuts at: 5171 6327 5171 Size: 1156 5958 ApoI r'AATT_y Cuts at: 1493 2186 2280 3466 3488 4219 4423 4649 4801 Size: 693 94 1186 22 731 204 226 152 Cuts at: 4801 6910 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1186 731 693 226 204 152 94 22 AseI AT'TA_AT Cuts at: 160 1032 5668 160 Size: 872 4636 1606 Fragments arranged by size: 4636 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 2450 4439 1870 Size: 73 507 1989 4545 Fragments arranged by size: 4545 1989 507 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2419 2452 2419 Size: 33 7081 BamHI G'GATC_C Cuts at: 3657 4444 4811 3657 Size: 787 367 5960 Fragments arranged by size: 5960 787 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4435 5890 618 Size: 325 217 627 2648 1455 1842 Fragments arranged by size: 2648 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2137 4269 4433 1391 Size: 746 2132 164 4072 Fragments arranged by size: 4072 2132 746 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 2162 3290 928 Size: 971 175 88 1128 4752 Fragments arranged by size: 4752 1128 971 175 88 BbvCI CC'TCA_GC Cuts at: 3017 3017 Size: 7114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 6216 5006 Size: 1210 5904 BclI T'GATC_A Cuts at: 1548 2103 3124 3780 1548 Size: 555 1021 656 4882 Fragments arranged by size: 4882 1021 656 555 BfrBI ATG'CAT Cuts at: 2743 2743 Size: 7114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 5725 136 Size: 107 122 71 631 605 454 3599 1525 Fragments arranged by size: 3599 1525 631 605 454 122 107 71 BglII A'GATC_T Cuts at: 7109 7109 Size: 7114 BlpI GC'TnA_GC Cuts at: 2032 2526 3338 4531 2032 Size: 494 812 1193 4615 Fragments arranged by size: 4615 1193 812 494 Bme1580I G_kGCm'C Cuts at: 1790 5175 6331 1790 Size: 3385 1156 2573 Fragments arranged by size: 3385 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4398 1318 Size: 3080 4034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 2427 4845 Size: 416 53 83 399 24 621 389 2418 Cuts at: 4845 5803 442 Size: 958 1753 Fragments arranged by size: 2418 1753 958 621 416 399 389 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4003 4003 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 3365 4283 5774 1239 Size: 884 1242 918 1491 2579 Fragments arranged by size: 2579 1491 1242 918 884 Bpu10I CC'TnA_GC Cuts at: 3017 3017 Size: 7114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3383 3515 3524 5239 6104 6402 3383 Size: 132 9 1715 865 298 4095 Fragments arranged by size: 4095 1715 865 298 132 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5777 882 Size: 617 4278 2219 Fragments arranged by size: 4278 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4905 493 Size: 711 3701 2702 Fragments arranged by size: 3701 2702 711 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 4921 5303 5938 Size: 53 83 186 936 546 2842 382 635 Cuts at: 5938 275 Size: 1451 Fragments arranged by size: 2842 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5547 5945 6219 6366 6777 5547 Size: 398 274 147 411 5884 Fragments arranged by size: 5884 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4279 4309 1178 Size: 30 165 30 2876 30 3983 Fragments arranged by size: 3983 2876 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3034 3476 4361 1186 Size: 1848 442 885 3939 Fragments arranged by size: 3939 1848 885 442 BseYI C'CCAG_C Cuts at: 3516 6317 6813 3516 Size: 2801 496 3817 Fragments arranged by size: 3817 2801 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 3429 3936 1163 Size: 573 1167 526 507 4341 Fragments arranged by size: 4341 1167 573 526 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4564 5325 6353 1540 Size: 275 186 2563 761 1028 2301 Fragments arranged by size: 2563 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2137 2382 2817 4269 5175 5260 6331 2137 Size: 245 435 1452 906 85 1071 2920 Fragments arranged by size: 2920 1452 1071 906 435 245 85 BsmI GAATG_Cn' Cuts at: 4625 4718 4625 Size: 93 7021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2382 2817 4269 4433 5175 5260 Size: 399 347 245 435 1452 164 742 85 Cuts at: 5260 6331 1391 Size: 1071 2174 Fragments arranged by size: 2174 1452 1071 742 435 399 347 245 164 85 BspEI T'CCGG_A Cuts at: 6777 6777 Size: 7114 BspHI T'CATG_A Cuts at: 5003 6733 5003 Size: 1730 5384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4469 844 Size: 3625 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2269 5001 1394 Size: 429 446 2732 3507 Fragments arranged by size: 3507 2732 446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2618 3689 3936 5609 5783 65 Size: 1183 1370 1071 247 1673 174 1396 Fragments arranged by size: 1673 1396 1370 1183 1071 247 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5758 5945 1700 Size: 110 3948 187 2869 Fragments arranged by size: 3948 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 5174 6186 5174 Size: 1012 6102 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7114 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstXI CCAn_nnnn'nTGG Cuts at: 2680 2680 Size: 7114 BstYI r'GATC_y Cuts at: 1972 3657 4057 4444 4811 5196 5213 6665 6751 Size: 1685 400 387 367 385 17 1452 86 Cuts at: 6751 6763 7109 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1685 1452 400 387 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2581 3848 2581 Size: 1267 5847 BtgI C'CryG_G Cuts at: 513 1065 2069 2492 513 Size: 552 1004 423 5135 Fragments arranged by size: 5135 1004 552 423 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6375 Fragments arranged by size: 6375 683 56 BtsI GCAGTG_nn' Cuts at: 4193 4630 5424 5444 4193 Size: 437 794 20 5863 Fragments arranged by size: 5863 794 437 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7079 DraI TTT'AAA Cuts at: 3471 4418 3471 Size: 947 6167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6121 809 Size: 507 4805 1802 Fragments arranged by size: 4805 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2127 2463 Size: 54 1425 50 275 18 288 9 336 Cuts at: 2463 4561 5450 7079 8 Size: 2098 889 1629 43 Fragments arranged by size: 2098 1629 1425 889 336 288 275 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4561 1537 Size: 275 2749 4090 Fragments arranged by size: 4090 2749 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2998 3635 3870 4371 5044 1353 Size: 1645 637 235 501 673 3423 Fragments arranged by size: 3423 1645 673 637 501 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3470 5701 6075 6221 387 Size: 1064 138 1881 2231 374 146 1280 Fragments arranged by size: 2231 1881 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2135 4267 2135 Size: 2132 4982 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 3050 3365 3884 4283 5177 5774 Size: 393 491 927 315 519 399 894 597 Cuts at: 5774 6561 6829 1239 Size: 787 268 1524 Fragments arranged by size: 1524 927 894 787 597 519 491 399 393 315 268 EcoNI CCTnn'n_nnAGG Cuts at: 3170 3170 Size: 7114 EcoO109I rG'GnC_Cy Cuts at: 2944 3627 2944 Size: 683 6431 EcoRI G'AATT_C Cuts at: 2280 4423 4801 2280 Size: 2143 378 4593 Fragments arranged by size: 4593 2143 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2685 3115 2685 Size: 430 6684 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 2908 6261 1678 Size: 1230 3353 2531 Fragments arranged by size: 3353 2531 1230 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 3745 3777 5755 Size: 32 135 32 458 32 1683 32 1978 Cuts at: 5755 5787 5829 5861 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 1683 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3394 4458 4704 669 Size: 988 281 1383 73 1064 246 3079 Fragments arranged by size: 3079 1383 1064 988 281 246 73 HindIII A'AGCT_T Cuts at: 748 3242 748 Size: 2494 4620 HpaI GTT'AAC Cuts at: 3321 4704 3321 Size: 1383 5731 KpnI G_GTAC'C Cuts at: 4439 4439 Size: 7114 MfeI C'AATT_G Cuts at: 4713 4713 Size: 7114 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3576 6228 6412 1305 Size: 2271 2652 184 2007 Fragments arranged by size: 2652 2271 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2465 10 Size: 54 1425 343 633 4659 Fragments arranged by size: 4659 1425 633 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 4236 5072 5431 5590 518 Size: 648 1578 1492 836 359 159 2042 Fragments arranged by size: 2042 1578 1492 836 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2594 2651 3388 3786 4530 5207 6355 Size: 537 451 57 737 398 744 677 1148 Cuts at: 6355 6600 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 744 737 677 537 451 398 245 57 NcoI C'CATG_G Cuts at: 513 1065 2069 2492 513 Size: 552 1004 423 5135 Fragments arranged by size: 5135 1004 552 423 NdeI CA'TA_TG Cuts at: 387 387 Size: 7114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NsiI A_TGCA'T Cuts at: 2745 2745 Size: 7114 NspI r_CATG'y Cuts at: 1132 1229 2743 2747 4472 6017 1132 Size: 97 1514 4 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 1514 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7114 PciI A'CATG_T Cuts at: 6013 6013 Size: 7114 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7114 PfoI T'CCnGG_A Cuts at: 2679 3653 2679 Size: 974 6140 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2228 2260 5183 5215 6696 6728 2228 Size: 32 2923 32 1481 32 2614 Fragments arranged by size: 2923 2614 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2944 3627 2944 Size: 683 6431 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7114 PsiI TTA'TAA Cuts at: 4684 4684 Size: 7114 PspXI vC'TCGA_Gb Cuts at: 2450 2450 Size: 7114 PstI C_TGCA'G Cuts at: 830 2411 3379 4188 4466 830 Size: 1581 968 809 278 3478 Fragments arranged by size: 3478 1581 968 809 278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 2143 2594 2651 3388 3786 2143 Size: 451 57 737 398 5471 Fragments arranged by size: 5471 737 451 398 57 SacI G_AGCT'C Cuts at: 2137 4269 2137 Size: 2132 4982 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SapI GCTCTTCn'nnn_ Cuts at: 3635 3635 Size: 7114 SbfI CC_TGCA'GG Cuts at: 4466 4466 Size: 7114 ScaI AGT'ACT Cuts at: 2309 3586 4121 5362 7074 2309 Size: 1277 535 1241 1712 2349 Fragments arranged by size: 2349 1712 1277 1241 535 SfcI C'TryA_G Cuts at: 826 1045 2407 2998 3070 3375 4184 4462 5597 Size: 219 1362 591 72 305 809 278 1135 Cuts at: 5597 6278 6469 826 Size: 681 191 1471 Fragments arranged by size: 1471 1362 1135 809 681 591 305 278 219 191 72 SfiI GGCCn_nnn'nGGCC Cuts at: 2126 2126 Size: 7114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7114 SmaI CCC'GGG Cuts at: 1872 4441 1872 Size: 2569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2450 3398 3530 3539 5218 6119 Size: 197 926 507 948 132 9 1679 901 Cuts at: 6119 6381 820 Size: 262 1553 Fragments arranged by size: 1679 1553 948 926 901 507 262 197 132 9 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 2743 4472 1132 Size: 1611 1729 3774 Fragments arranged by size: 3774 1729 1611 SspI AAT'ATT Cuts at: 5 52 3038 4006 4144 5038 5 Size: 47 2986 968 138 894 2081 Fragments arranged by size: 2986 2081 968 894 138 47 StuI AGG'CCT Cuts at: 1517 2542 1517 Size: 1025 6089 StyI C'CwwG_G Cuts at: 513 1065 2069 2210 2492 2925 4553 513 Size: 552 1004 141 282 433 1628 3074 Fragments arranged by size: 3074 1628 1004 552 433 282 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4226 5141 5158 5311 5496 5941 325 Size: 1255 2646 915 17 153 185 445 1498 Fragments arranged by size: 2646 1498 1255 915 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 3584 3633 Size: 274 80 33 51 1443 330 1277 49 Cuts at: 3633 4119 5360 7072 96 Size: 486 1241 1712 138 Fragments arranged by size: 1712 1443 1277 1241 486 330 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7074 7106 7074 Size: 32 7082 Tth111I GACn'n_nGTC Cuts at: 1316 3308 1316 Size: 1992 5122 XbaI T'CTAG_A Cuts at: 3457 4450 3457 Size: 993 6121 XhoI C'TCGA_G Cuts at: 1943 2450 1943 Size: 507 6607 XmaI C'CCGG_G Cuts at: 1870 4439 1870 Size: 2569 4545 XmnI GAAnn'nnTTC Cuts at: 2732 4290 5243 2732 Size: 1558 953 4603 Fragments arranged by size: 4603 1558 953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2080 4922 276 Size: 53 83 186 936 546 2842 2468 Fragments arranged by size: 2842 2468 936 546 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI