Restriction map for
pFN21AA0231
(Linear) (Six-base) MAPSORT of: KIAA0231.seq Check: 8703 from: 1 to: 2409
KIAA0231.orf
With 182 enzymes: SgfI *
November 13, 2006 11:20 ..
AatII G_ACGT'C
Cuts at: 0 77 2409
Size: 77 2332
AccI GT'mk_AC
Cuts at: 0 1430 2409
Size: 1430 979
AclI AA'CG_TT
Cuts at: 0 2382 2409
Size: 2382 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1879 2409
Size: 1045 834 530
Fragments arranged by size:
1045 834 530
AfeI AGC'GCT
Cuts at: 0 901 2409
Size: 901 1508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2135 2194 2409
Size: 2135 59 215
Fragments arranged by size:
2135 215 59
AlwNI CAG_nnn'CTG
Cuts at: 0 520 1695 2100 2409
Size: 520 1175 405 309
Fragments arranged by size:
1175 520 405 309
ApoI r'AATT_y
Cuts at: 0 181 275 1461 1483 2214 2409
Size: 181 94 1186 22 731 195
Fragments arranged by size:
1186 731 195 181 94 22
AvaI C'yCGr_G
Cuts at: 0 445 2409
Size: 445 1964
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 414 447 2409
Size: 414 33 1962
Fragments arranged by size:
1962 414 33
BamHI G'GATC_C
Cuts at: 0 1652 2409
Size: 1652 757
BanII G_rGCy'C
Cuts at: 0 132 2264 2409
Size: 132 2132 145
Fragments arranged by size:
2132 145 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 157 1285 2409
Size: 69 88 1128 1124
Fragments arranged by size:
1128 1124 88 69
BbvCI CC'TCA_GC
Cuts at: 0 1012 2409
Size: 1012 1397
BclI T'GATC_A
Cuts at: 0 98 1119 1775 2409
Size: 98 1021 656 634
Fragments arranged by size:
1021 656 634 98
BfrBI ATG'CAT
Cuts at: 0 738 2409
Size: 738 1671
BglI GCCn_nnn'nGGC
Cuts at: 0 121 2409
Size: 121 2288
BlpI GC'TnA_GC
Cuts at: 0 27 521 1333 2409
Size: 27 494 812 1076
Fragments arranged by size:
1076 812 494 27
BmgBI CAC'GTC
Cuts at: 0 2393 2409
Size: 2393 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 33 422 2409
Size: 33 389 1987
Fragments arranged by size:
1987 389 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1998 2409
Size: 1998 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1360 2278 2409
Size: 118 1242 918 131
Fragments arranged by size:
1242 918 131 118
Bpu10I CC'TnA_GC
Cuts at: 0 1012 2409
Size: 1012 1397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1510 1519 2409
Size: 1378 132 9 890
Fragments arranged by size:
1378 890 132 9
BsaHI Gr'CG_yC
Cuts at: 0 74 2409
Size: 74 2335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2274 2304 2409
Size: 2274 30 105
Fragments arranged by size:
2274 105 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1029 1471 2356 2409
Size: 1029 442 885 53
Fragments arranged by size:
1029 885 442 53
BseYI C'CCAG_C
Cuts at: 0 1511 2409
Size: 1511 898
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1424 1931 2409
Size: 898 526 507 478
Fragments arranged by size:
898 526 507 478
BsiHKAI G_wGCw'C
Cuts at: 0 132 377 812 2264 2409
Size: 132 245 435 1452 145
Fragments arranged by size:
1452 435 245 145 132
Bsp1286I G_dGCh'C
Cuts at: 0 132 377 812 2264 2409
Size: 132 245 435 1452 145
Fragments arranged by size:
1452 435 245 145 132
BsrBI CCG'CTC
Cuts at: 0 264 2409
Size: 264 2145
BsrDI GCAATG_nn'
Cuts at: 0 613 1684 1931 2409
Size: 613 1071 247 478
Fragments arranged by size:
1071 613 478 247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 2409
Size: 675 1734
BstYI r'GATC_y
Cuts at: 0 1652 2052 2409
Size: 1652 400 357
Fragments arranged by size:
1652 400 357
Bsu36I CC'TnA_GG
Cuts at: 0 576 1843 2409
Size: 576 1267 566
Fragments arranged by size:
1267 576 566
BtgI C'CryG_G
Cuts at: 0 64 487 2409
Size: 64 423 1922
Fragments arranged by size:
1922 423 64
BtsI GCAGTG_nn'
Cuts at: 0 2188 2409
Size: 2188 221
DraI TTT'AAA
Cuts at: 0 1466 2409
Size: 1466 943
EaeI y'GGCC_r
Cuts at: 0 113 122 458 2409
Size: 113 9 336 1951
Fragments arranged by size:
1951 336 113 9
EarI CTCTTCn'nnn_
Cuts at: 0 993 1630 1865 2366 2409
Size: 993 637 235 501 43
Fragments arranged by size:
993 637 501 235 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1465 2409
Size: 1465 944
EcoICRI GAG'CTC
Cuts at: 0 130 2262 2409
Size: 130 2132 147
Fragments arranged by size:
2132 147 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1045 1360 1879 2278 2409
Size: 118 927 315 519 399 131
Fragments arranged by size:
927 519 399 315 131 118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1165 2409
Size: 1165 1244
EcoO109I rG'GnC_Cy
Cuts at: 0 939 1622 2409
Size: 939 683 787
Fragments arranged by size:
939 787 683
EcoRI G'AATT_C
Cuts at: 0 275 2409
Size: 275 2134
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 680 1110 2409
Size: 680 430 1299
Fragments arranged by size:
1299 680 430
HaeII r_GCGC'y
Cuts at: 0 903 2409
Size: 903 1506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 1740 1772 2409
Size: 25 32 1683 32 637
Fragments arranged by size:
1683 637 32 32 25
HincII GTy'rAC
Cuts at: 0 1316 1389 2409
Size: 1316 73 1020
Fragments arranged by size:
1316 1020 73
HindIII A'AGCT_T
Cuts at: 0 1237 2409
Size: 1237 1172
HpaI GTT'AAC
Cuts at: 0 1316 2409
Size: 1316 1093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1571 2409
Size: 1571 838
MscI TGG'CCA
Cuts at: 0 460 2409
Size: 460 1949
MslI CAynn'nnrTG
Cuts at: 0 739 2231 2409
Size: 739 1492 178
Fragments arranged by size:
1492 739 178
MspA1I CmG'CkG
Cuts at: 0 138 589 646 1383 1781 2409
Size: 138 451 57 737 398 628
Fragments arranged by size:
737 628 451 398 138 57
NcoI C'CATG_G
Cuts at: 0 64 487 2409
Size: 64 423 1922
Fragments arranged by size:
1922 423 64
NsiI A_TGCA'T
Cuts at: 0 740 2409
Size: 740 1669
NspI r_CATG'y
Cuts at: 0 738 742 2409
Size: 738 4 1667
Fragments arranged by size:
1667 738 4
PfoI T'CCnGG_A
Cuts at: 0 674 1648 2409
Size: 674 974 761
Fragments arranged by size:
974 761 674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 2409
Size: 223 32 2154
Fragments arranged by size:
2154 223 32
PpuMI rG'GwC_Cy
Cuts at: 0 939 1622 2409
Size: 939 683 787
Fragments arranged by size:
939 787 683
PspXI vC'TCGA_Gb
Cuts at: 0 445 2409
Size: 445 1964
PstI C_TGCA'G
Cuts at: 0 406 1374 2183 2409
Size: 406 968 809 226
Fragments arranged by size:
968 809 406 226
PvuII CAG'CTG
Cuts at: 0 138 589 646 1383 1781 2409
Size: 138 451 57 737 398 628
Fragments arranged by size:
737 628 451 398 138 57
SacI G_AGCT'C
Cuts at: 0 132 2264 2409
Size: 132 2132 145
Fragments arranged by size:
2132 145 132
SapI GCTCTTCn'nnn_
Cuts at: 0 1630 2409
Size: 1630 779
ScaI AGT'ACT
Cuts at: 0 304 1581 2116 2409
Size: 304 1277 535 293
Fragments arranged by size:
1277 535 304 293
SfcI C'TryA_G
Cuts at: 0 402 993 1065 1370 2179 2409
Size: 402 591 72 305 809 230
Fragments arranged by size:
809 591 402 305 230 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 121 2409
Size: 121 2288
SmlI C'TyrA_G
Cuts at: 0 445 1393 1525 1534 2409
Size: 445 948 132 9 875
Fragments arranged by size:
948 875 445 132 9
SphI G_CATG'C
Cuts at: 0 738 2409
Size: 738 1671
SspI AAT'ATT
Cuts at: 0 1033 2001 2139 2409
Size: 1033 968 138 270
Fragments arranged by size:
1033 968 270 138
StuI AGG'CCT
Cuts at: 0 537 2409
Size: 537 1872
StyI C'CwwG_G
Cuts at: 0 64 205 487 920 2409
Size: 64 141 282 433 1489
Fragments arranged by size:
1489 433 282 141 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2221 2409
Size: 2221 188
TatI w'GTAC_w
Cuts at: 0 302 1579 1628 2114 2409
Size: 302 1277 49 486 295
Fragments arranged by size:
1277 486 302 295 49
Tth111I GACn'n_nGTC
Cuts at: 0 1303 2409
Size: 1303 1106
XbaI T'CTAG_A
Cuts at: 0 1452 2409
Size: 1452 957
XhoI C'TCGA_G
Cuts at: 0 445 2409
Size: 445 1964
XmnI GAAnn'nnTTC
Cuts at: 0 727 2285 2409
Size: 727 1558 124
Fragments arranged by size:
1558 727 124
ZraI GAC'GTC
Cuts at: 0 75 2409
Size: 75 2334
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AlfI AlwNI ApoI
AvaI BaeI BamHI BanII BbsI BbvCI BclI BfrBI
BglI BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI
BsrDI BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NcoI NsiI NspI PfoI PpiI PpuMI PspXI
PstI PvuII SacI SapI ScaI SfcI SfiI SmlI
SphI SspI StuI StyI TaqII TatI Tth111I XbaI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AflIII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BanI BbeI
BcgI BciVI BglII Bme1580I BmtI BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EcoRV FseI
FspI FspAI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TstI XcmI
XmaI