(Circular) (Six-base) MAPSORT of: pFN21AA0226.seq Check: 3021 from: 1 to: 7486 pFN21AA0226 7486 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5296 278 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4829 1937 Size: 2892 4594 Acc65I G'GTAC_C Cuts at: 3237 4807 3237 Size: 1570 5916 AclI AA'CG_TT Cuts at: 1563 5613 5986 1563 Size: 4050 373 3063 Fragments arranged by size: 4050 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3340 3494 3791 4156 5549 6933 7201 1632 Size: 1708 154 297 365 1393 1384 268 1917 Fragments arranged by size: 1917 1708 1393 1384 365 297 268 154 AfeI AGC'GCT Cuts at: 4752 4752 Size: 7486 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7289 AflIII A'CryG_T Cuts at: 1203 4332 6385 7362 1203 Size: 3129 2053 977 1327 Fragments arranged by size: 3129 2053 1327 977 AgeI A'CCGG_T Cuts at: 6317 6317 Size: 7486 AhdI GACnn_n'nnGTC Cuts at: 6215 6215 Size: 7486 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7486 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3151 1184 Size: 1967 5519 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3245 3277 3245 Size: 32 7454 AlwNI CAG_nnn'CTG Cuts at: 2291 2338 2443 2666 2915 3472 4708 5229 6801 Size: 47 105 223 249 557 1236 521 1572 Cuts at: 6801 2291 Size: 2976 Fragments arranged by size: 2976 1572 1236 557 521 249 223 105 47 ApaI G_GGCC'C Cuts at: 3952 4464 3952 Size: 512 6974 ApaLI G'TGCA_C Cuts at: 2138 5543 6699 2138 Size: 3405 1156 2925 Fragments arranged by size: 3405 2925 1156 ApoI r'AATT_y Cuts at: 1493 2276 3641 4795 5021 5173 7282 1493 Size: 783 1365 1154 226 152 2109 1697 Fragments arranged by size: 2109 1697 1365 1154 783 226 152 AseI AT'TA_AT Cuts at: 160 1032 6040 160 Size: 872 5008 1606 Fragments arranged by size: 5008 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2820 4811 1870 Size: 73 309 568 1991 4545 Fragments arranged by size: 4545 1991 568 309 73 BamHI G'GATC_C Cuts at: 4816 5183 4816 Size: 367 7119 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 3237 3495 4807 6262 Size: 325 217 627 381 1069 258 1312 1455 Cuts at: 6262 618 Size: 1842 Fragments arranged by size: 1842 1455 1312 1069 627 381 325 258 217 BanII G_rGCy'C Cuts at: 1391 2417 2711 3082 3768 3952 4464 4479 4587 Size: 1026 294 371 686 184 512 15 108 Cuts at: 4587 4743 4805 1391 Size: 156 62 4072 Fragments arranged by size: 4072 1026 686 512 371 294 184 156 108 62 15 BbeI G_GCGC'C Cuts at: 3499 3499 Size: 7486 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3274 3453 928 Size: 971 1375 179 4961 Fragments arranged by size: 4961 1375 971 179 BbvCI CC'TCA_GC Cuts at: 3747 3747 Size: 7486 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3679 3713 5677 5711 1903 Size: 34 50 34 1658 34 1964 34 3678 Fragments arranged by size: 3678 1964 1658 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3049 5378 6588 3049 Size: 2329 1210 3947 Fragments arranged by size: 3947 2329 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7486 BfrBI ATG'CAT Cuts at: 4512 4512 Size: 7486 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4136 6097 136 Size: 107 122 71 631 605 2464 1961 1525 Fragments arranged by size: 2464 1961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2558 4218 7481 2558 Size: 1660 3263 2563 Fragments arranged by size: 3263 2563 1660 BlpI GC'TnA_GC Cuts at: 2490 3543 4473 4903 2490 Size: 1053 930 430 5073 Fragments arranged by size: 5073 1053 930 430 Bme1580I G_kGCm'C Cuts at: 1790 2142 2538 2616 3952 4264 4464 5547 6703 Size: 352 396 78 1336 312 200 1083 1156 Cuts at: 6703 1790 Size: 2573 Fragments arranged by size: 2573 1336 1156 1083 396 352 312 200 78 BmgBI CAC'GTC Cuts at: 1318 2617 3741 1318 Size: 1299 1124 5063 Fragments arranged by size: 5063 1299 1124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 2648 3574 Size: 416 53 83 399 24 1034 197 926 Cuts at: 3574 3703 5217 6175 442 Size: 129 1514 958 1753 Fragments arranged by size: 1753 1514 1034 958 926 416 399 197 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2695 2875 3055 3066 3553 3833 4730 4796 Size: 1456 180 180 11 487 280 897 66 Cuts at: 4796 6146 1239 Size: 1350 2579 Fragments arranged by size: 2579 1456 1350 897 487 280 180 180 66 11 Bpu10I CC'TnA_GC Cuts at: 3247 3747 3247 Size: 500 6986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 3667 5611 6476 6774 2583 Size: 1084 1944 865 298 3295 Fragments arranged by size: 3295 1944 1084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6149 882 Size: 617 4650 2219 Fragments arranged by size: 4650 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3593 5277 493 Size: 711 915 1474 1684 2702 Fragments arranged by size: 2702 1684 1474 915 711 BsaBI GATnn'nnATC Cuts at: 4139 7480 4139 Size: 3341 4145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 5293 5675 6310 Size: 53 83 186 936 1963 1797 382 635 Cuts at: 6310 275 Size: 1451 Fragments arranged by size: 1963 1797 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 3883 4047 5919 6317 6591 6738 7149 2088 Size: 1795 164 1872 398 274 147 411 2425 Fragments arranged by size: 2425 1872 1795 411 398 274 164 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3029 3059 3800 3830 1178 Size: 30 165 30 1626 30 741 30 4834 Fragments arranged by size: 4834 1626 741 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2878 3440 3443 4748 4751 1186 Size: 1692 562 3 1305 3 3921 Fragments arranged by size: 3921 1692 1305 562 3 3 BseYI C'CCAG_C Cuts at: 2235 3203 4145 4456 6689 7185 2235 Size: 968 942 311 2233 496 2536 Fragments arranged by size: 2536 2233 968 942 496 311 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2652 3716 4053 4265 1163 Size: 573 916 1064 337 212 4384 Fragments arranged by size: 4384 1064 916 573 337 212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4936 5697 6725 1540 Size: 275 186 2935 761 1028 2301 Fragments arranged by size: 2935 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2711 3082 3248 3549 4479 4587 5547 5632 Size: 569 371 166 301 930 108 960 85 Cuts at: 5632 6703 2142 Size: 1071 2925 Fragments arranged by size: 2925 1071 960 930 569 371 301 166 108 85 BsmI GAATG_Cn' Cuts at: 2494 3352 4132 4997 5090 2494 Size: 858 780 865 93 4890 Fragments arranged by size: 4890 865 858 780 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2623 2623 Size: 7486 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2417 2538 2616 2711 3082 3248 Size: 399 352 275 121 78 95 371 166 Cuts at: 3248 3549 3768 3952 4264 4464 4479 4587 4743 Size: 301 219 184 312 200 15 108 156 Cuts at: 4743 4805 5547 5632 6703 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 352 312 301 275 219 200 184 166 156 121 108 95 85 78 62 15 BspEI T'CCGG_A Cuts at: 4047 7149 4047 Size: 3102 4384 BspHI T'CATG_A Cuts at: 5375 7105 5375 Size: 1730 5756 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2970 3528 4215 4841 844 Size: 2126 558 687 626 3489 Fragments arranged by size: 3489 2126 687 626 558 BsrBI CCG'CTC Cuts at: 1394 1823 2419 4669 5373 1394 Size: 429 596 2250 704 3507 Fragments arranged by size: 3507 2250 704 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2863 3063 3123 3145 5981 6155 65 Size: 1183 1615 200 60 22 2836 174 1396 Fragments arranged by size: 2836 1615 1396 1183 200 174 60 22 BsrFI r'CCGG_y Cuts at: 1700 1810 6130 6317 1700 Size: 110 4320 187 2869 Fragments arranged by size: 4320 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3213 96 Size: 3117 4369 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7486 BssSI C'ACGA_G Cuts at: 2141 2408 4121 5546 6558 2141 Size: 267 1713 1425 1012 3069 Fragments arranged by size: 3069 1713 1425 1012 267 BstAPI GCAn_nnn'nTGC Cuts at: 794 2212 4518 794 Size: 1418 2306 3762 Fragments arranged by size: 3762 2306 1418 BstBI TT'CG_AA Cuts at: 6290 6290 Size: 7486 BstEII G'GTnAC_C Cuts at: 1184 3562 1184 Size: 2378 5108 BstYI r'GATC_y Cuts at: 1972 2558 3118 3462 3483 4218 4816 5183 5568 Size: 586 560 344 21 735 598 367 385 Cuts at: 5568 5585 7037 7123 7135 7481 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 735 598 586 560 385 367 346 344 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2929 2929 Size: 7486 BtgI C'CryG_G Cuts at: 513 1065 3759 4008 4743 513 Size: 552 2694 249 735 3256 Fragments arranged by size: 3256 2694 735 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6747 Fragments arranged by size: 6747 683 56 BtsI GCAGTG_nn' Cuts at: 2164 2221 2339 2352 3193 3721 3869 5002 5796 Size: 57 118 13 841 528 148 1133 794 Cuts at: 5796 5816 2164 Size: 20 3834 Fragments arranged by size: 3834 1133 841 794 528 148 118 57 20 13 ClaI AT'CG_AT Cuts at: 1554 2382 1554 Size: 828 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7451 DraI TTT'AAA Cuts at: 4790 4790 Size: 7486 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 6493 809 Size: 507 999 4178 1802 Fragments arranged by size: 4178 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2799 3756 Size: 54 1425 50 275 18 492 477 957 Cuts at: 3756 4011 4137 4353 4695 4933 5822 7451 8 Size: 255 126 216 342 238 889 1629 43 Fragments arranged by size: 1629 1425 957 889 492 477 342 275 255 238 216 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4933 1537 Size: 275 3121 4090 Fragments arranged by size: 4090 3121 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2929 3435 4241 4602 5416 1353 Size: 1576 506 806 361 814 3423 Fragments arranged by size: 3423 1576 814 806 506 361 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3476 6073 6447 6593 387 Size: 1064 138 1887 2597 374 146 1280 Fragments arranged by size: 2597 1887 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2709 3080 4477 4585 2709 Size: 371 1397 108 5610 Fragments arranged by size: 5610 1397 371 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2695 2875 3055 3066 3340 3494 3553 Size: 393 1063 180 180 11 274 154 59 Cuts at: 3553 3791 3833 4156 4730 4796 5549 6146 6933 Size: 238 42 323 574 66 753 597 787 Cuts at: 6933 7201 1239 Size: 268 1524 Fragments arranged by size: 1524 1063 787 753 597 574 393 323 274 268 238 180 180 154 66 59 42 11 EcoNI CCTnn'n_nnAGG Cuts at: 3338 3425 4400 3338 Size: 87 975 6424 Fragments arranged by size: 6424 975 87 EcoO109I rG'GnC_Cy Cuts at: 2508 2786 3089 3161 3949 4204 4405 4460 4461 Size: 278 303 72 788 255 201 55 1 Cuts at: 4461 2508 Size: 5533 Fragments arranged by size: 5533 788 303 278 255 201 72 55 1 EcoRI G'AATT_C Cuts at: 4795 5173 4795 Size: 378 7108 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2580 4186 2580 Size: 1606 5880 FspI TGC'GCA Cuts at: 4160 5992 4160 Size: 1832 5654 HaeII r_GCGC'y Cuts at: 1678 3499 4754 6633 1678 Size: 1821 1255 1879 2531 Fragments arranged by size: 2531 1879 1821 1255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2253 2285 2543 2575 2601 Size: 32 135 32 681 32 258 32 26 Cuts at: 2601 2633 3273 3305 3605 3637 4029 4061 4500 Size: 32 640 32 300 32 392 32 439 Cuts at: 4500 4532 6127 6159 6201 6233 1373 Size: 32 1595 32 42 32 2626 Fragments arranged by size: 2626 1595 681 640 439 392 300 258 135 42 32 32 32 32 32 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 4830 5076 669 Size: 988 281 2892 246 3079 Fragments arranged by size: 3079 2892 988 281 246 HindIII A'AGCT_T Cuts at: 748 3553 748 Size: 2805 4681 HpaI GTT'AAC Cuts at: 5076 5076 Size: 7486 KasI G'GCGC_C Cuts at: 3495 3495 Size: 7486 KpnI G_GTAC'C Cuts at: 3241 4811 3241 Size: 1570 5916 MfeI C'AATT_G Cuts at: 5085 5085 Size: 7486 MluI A'CGCG_T Cuts at: 7362 7362 Size: 7486 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6600 6784 1305 Size: 5295 184 2007 Fragments arranged by size: 5295 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 3758 4013 4139 4355 Size: 54 1425 343 969 957 255 126 216 Cuts at: 4355 4697 10 Size: 342 2799 Fragments arranged by size: 2799 1425 969 957 343 342 255 216 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 2324 4125 5444 5803 5962 518 Size: 648 969 189 1801 1319 359 159 2042 Fragments arranged by size: 2042 1801 1319 969 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2838 2962 3324 4029 4315 4745 4902 5579 Size: 1232 124 362 705 286 430 157 677 Cuts at: 5579 6727 6972 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1232 1148 705 677 430 362 286 245 157 124 NarI GG'CG_CC Cuts at: 3496 3496 Size: 7486 NcoI C'CATG_G Cuts at: 513 1065 3759 513 Size: 552 2694 4240 Fragments arranged by size: 4240 2694 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7486 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7486 NotI GC'GGCC_GC Cuts at: 4933 4933 Size: 7486 NsiI A_TGCA'T Cuts at: 4514 4514 Size: 7486 NspI r_CATG'y Cuts at: 1132 1229 3262 4336 4516 4844 6389 1132 Size: 97 2033 1074 180 328 1545 2229 Fragments arranged by size: 2229 2033 1545 1074 328 180 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7486 PciI A'CATG_T Cuts at: 4332 6385 4332 Size: 2053 5433 PflMI CCAn_nnn'nTGG Cuts at: 1108 2579 1108 Size: 1471 6015 PfoI T'CCnGG_A Cuts at: 3082 3465 3825 4773 3082 Size: 383 360 948 5795 Fragments arranged by size: 5795 948 383 360 PmeI GTTT'AAAC Cuts at: 4790 4790 Size: 7486 PmlI CAC'GTG Cuts at: 2119 3593 2119 Size: 1474 6012 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2857 2889 3665 3697 5555 5587 7068 7100 2857 Size: 32 776 32 1858 32 1481 32 3243 Fragments arranged by size: 3243 1858 1481 776 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2508 4204 4405 2508 Size: 1696 201 5589 Fragments arranged by size: 5589 1696 201 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7486 PsiI TTA'TAA Cuts at: 5056 5056 Size: 7486 PspOMI G'GGCC_C Cuts at: 3948 4460 3948 Size: 512 6974 PstI C_TGCA'G Cuts at: 830 2205 2217 2334 4677 4838 830 Size: 1375 12 117 2343 161 3478 Fragments arranged by size: 3478 2343 1375 161 117 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7486 PvuII CAG'CTG Cuts at: 2838 2962 3324 4315 2838 Size: 124 362 991 6009 Fragments arranged by size: 6009 991 362 124 SacI G_AGCT'C Cuts at: 2711 3082 4479 4587 2711 Size: 371 1397 108 5610 Fragments arranged by size: 5610 1397 371 108 SacII CC_GC'GG Cuts at: 4746 4746 Size: 7486 SalI G'TCGA_C Cuts at: 1936 4828 1936 Size: 2892 4594 SbfI CC_TGCA'GG Cuts at: 4838 4838 Size: 7486 ScaI AGT'ACT Cuts at: 2716 4103 4181 5734 7446 2716 Size: 1387 78 1553 1712 2756 Fragments arranged by size: 2756 1712 1553 1387 78 SfcI C'TryA_G Cuts at: 826 1045 2201 2213 2330 4267 4673 4834 5969 Size: 219 1156 12 117 1937 406 161 1135 Cuts at: 5969 6650 6841 826 Size: 681 191 1471 Fragments arranged by size: 1937 1471 1156 1135 681 406 219 191 161 117 12 SfoI GGC'GCC Cuts at: 3497 3497 Size: 7486 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7486 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7486 SmaI CCC'GGG Cuts at: 1872 4813 1872 Size: 2941 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 3682 5590 6491 6753 820 Size: 197 926 655 1084 1908 901 262 1553 Fragments arranged by size: 1908 1553 1084 926 901 655 262 197 SnaBI TAC'GTA Cuts at: 493 5277 493 Size: 4784 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7486 SphI G_CATG'C Cuts at: 1132 4516 4844 1132 Size: 3384 328 3774 Fragments arranged by size: 3774 3384 328 SspI AAT'ATT Cuts at: 5 52 5410 5 Size: 47 5358 2081 Fragments arranged by size: 5358 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7486 StyI C'CwwG_G Cuts at: 513 1065 3759 4200 4356 4925 513 Size: 552 2694 441 156 569 3074 Fragments arranged by size: 3074 2694 569 552 441 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3217 5513 5530 5683 5868 6313 325 Size: 1255 1637 2296 17 153 185 445 1498 Fragments arranged by size: 2296 1637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2714 3213 4101 Size: 274 80 33 51 1443 737 499 888 Cuts at: 4101 4179 4416 5732 7444 96 Size: 78 237 1316 1712 138 Fragments arranged by size: 1712 1443 1316 888 737 499 274 237 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3029 3061 3800 3832 7446 7478 3029 Size: 32 739 32 3614 32 3037 Fragments arranged by size: 3614 3037 739 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4382 1316 Size: 3066 4420 XbaI T'CTAG_A Cuts at: 4822 4822 Size: 7486 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2945 4363 4705 2809 Size: 136 1418 342 5590 Fragments arranged by size: 5590 1418 342 136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7486 XmaI C'CCGG_G Cuts at: 1870 4811 1870 Size: 2941 4545 XmnI GAAnn'nnTTC Cuts at: 4334 5615 4334 Size: 1281 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5294 276 Size: 53 83 186 936 3760 2468 Fragments arranged by size: 3760 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI