Restriction map for
pFN21AA0203
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvrII BanI BbeI BbrPI BbvCI
BcnI BlnI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BstAPI BstEII BstPI BstUI
Cfr10I ClaI CpoI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FauI FseI HaeII HapII HgaI
Hin1I HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflII 82 CTTAAG C^TTAAG
Alw44I 4238 GTGCAC G^TGCAC
AocI 1788 CCTCAGG CC^T[ACGT]AGG
ApaLI 4238 GTGCAC G^TGCAC
AseI 3073 ATTAAT AT^TAAT
AsnI 3073 ATTAAT AT^TAAT
AvaII 1116 GGACC G^G[AT]CC
AviII 4175 TGCGCA TGC^GCA
BamHI 2031 GGATCC G^GATCC
BcgI 3951 GCAAAATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1386 TGATCA T^GATCA
BfrI 82 CTTAAG C^TTAAG
BglI 4690 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1899 AGATCT A^GATCT
BsaI 3259 GAGACC GGTCTC(1/5)
Bst1107I 2432 GTATAC GTA^TAC
BstBI 3959 TTCGAA TT^CGAA
BstZ17I 2432 GTATAC GTA^TAC
Bsu36I 1788 CCTCAGG CC^T[ACGT]AGG
CfoI 4176 GCGC GCG^C
DraIII 124 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1788 CCTCAGG CC^T[ACGT]AGG
EcoNI 2703 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1386 TGATCA T^GATCA
FspI 4175 TGCGCA TGC^GCA
HhaI 4176 GCGC GCG^C
HinP1I 4176 GCGC G^CGC
MaeII 2095 ACGT A^CGT
NheI 1129 GCTAGC G^CTAGC
NspV 3959 TTCGAA TT^CGAA
PflMI 126 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4436 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3073 ATTAAT AT^TAAT
SacI 4124 GAGCTC GAGCT^C
SapI 3919 GAAGAGC GCTCTTC(1/4)
ScaI 850 AGTACT AGT^ACT
SexAI 125 ACCAGGT A^CC[AT]GGT
SfuI 3959 TTCGAA TT^CGAA
SphI 87 GCATGC GCATG^C
SspI 4342 AATATT AAT^ATT
SwaI 3744 ATTTAAAT ATTT^AAAT
Van91I 126 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 789 ACATGT A^C[GA][CT]GT
1344 ACATGT
BalI 461 TGGCCA TGG^CCA
596 TGGCCA
BanII 3245 GAGCCC G[GA]GC[CT]^C
4124 GAGCTC
BciVI 198 GGATAC GTATCC(6/5)
2334 GGATAC
BlpI 1490 GCTTAGC GC^T[ACGT]AGC
3362 GCTTAGC
BpmI 2072 CTCCAG CTGGAG(16/14)
469 CTGGAG
Bpu1102I 1490 GCTTAGC GC^T[ACGT]AGC
3362 GCTTAGC
BsmBI 2237 CGTCTC CGTCTC(1/5)
697 GAGACG
BspLU11I 789 ACATGT A^CATGT
1344 ACATGT
BspMI 252 ACCTGC ACCTGC(4/8)
4605 ACCTGC
BsrDI 4394 CATTGC GCAATG(2/0)
4309 GCAATG
BssSI 2861 CACGAG CACGAG(-5/-1)
3165 CACGAG
BstXI 710 CCACTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCAAGTCAGTGG
CelII 1490 GCTTAGC GC^T[ACGT]AGC
3362 GCTTAGC
Cfr13I 1116 GGACC G^G[ACGT]CC
1673 GGCCC
EcoT22I 153 ATGCAT ATGCA^T
1214 ATGCAT
HincII 22 GTTAAC GT[CT]^[GA]AC
762 GTTAAC
HindII 22 GTTAAC GT[CT]^[GA]AC
762 GTTAAC
HpaI 22 GTTAAC GTT^AAC
762 GTTAAC
MfeI 3389 CAATTG C^AATTG
4399 CAATTG
MluNI 461 TGGCCA TGG^CCA
596 TGGCCA
MscI 461 TGGCCA TGG^CCA
596 TGGCCA
MslI 1825 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2507 CATTAAAATG
MspA1I 4271 CCGCTG C[AC]G^C[GT]G
4369 CAGCGG
MunI 3389 CAATTG C^AATTG
4399 CAATTG
NdeI 4202 CATATG CA^TATG
4348 CATATG
NsiI 153 ATGCAT ATGCA^T
1214 ATGCAT
Sau96I 1116 GGACC G^G[ACGT]CC
1673 GGCCC
XbaI 3906 TCTAGA T^CTAGA
4639 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
BbsI BbvI BfaI BmyI BpuAI
BsaBI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MamI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI