(Circular) (Six-base) MAPSORT of: pFN21AA0203.seq Check: 5299 from: 1 to: 9478 pFN21AA0203 9478 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2266 2266 Size: 9478 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7288 278 Size: 53 83 186 936 5752 2468 Fragments arranged by size: 5752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4327 4438 5431 6821 1937 Size: 2390 111 993 1390 4594 Fragments arranged by size: 4594 2390 1390 993 111 Acc65I G'GTAC_C Cuts at: 6799 6799 Size: 9478 AclI AA'CG_TT Cuts at: 1563 7605 7978 1563 Size: 6042 373 3063 Fragments arranged by size: 6042 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3380 4417 4680 4877 4928 4973 5873 6141 Size: 1748 1037 263 197 51 45 900 268 Cuts at: 6141 6384 6447 6618 7541 8925 9193 1632 Size: 243 63 171 923 1384 268 1917 Fragments arranged by size: 1917 1748 1384 1037 923 900 268 268 263 243 197 171 63 51 45 AflII C'TTAA_G Cuts at: 820 1017 2087 820 Size: 197 1070 8211 Fragments arranged by size: 8211 1070 197 AflIII A'CryG_T Cuts at: 1203 2794 3349 8377 9354 1203 Size: 1591 555 5028 977 1327 Fragments arranged by size: 5028 1591 1327 977 555 AgeI A'CCGG_T Cuts at: 8309 8309 Size: 9478 AhdI GACnn_n'nnGTC Cuts at: 8207 8207 Size: 9478 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9478 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3858 1184 Size: 2674 6804 AlwNI CAG_nnn'CTG Cuts at: 2137 2819 3757 5216 7221 8793 2137 Size: 682 938 1459 2005 1572 2822 Fragments arranged by size: 2822 2005 1572 1459 938 682 ApaLI G'TGCA_C Cuts at: 6243 7535 8691 6243 Size: 1292 1156 7030 Fragments arranged by size: 7030 1292 1156 ApoI r'AATT_y Cuts at: 1493 2488 4234 4507 4739 5229 5299 5747 6787 Size: 995 1746 273 232 490 70 448 1040 Cuts at: 6787 7013 7165 9274 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1746 1697 1040 995 490 448 273 232 226 152 70 AseI AT'TA_AT Cuts at: 160 1032 5079 8032 160 Size: 872 4047 2953 1606 Fragments arranged by size: 4047 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9478 AvaI C'yCGr_G Cuts at: 1870 1943 6803 1870 Size: 73 4860 4545 Fragments arranged by size: 4860 4545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4332 4365 4332 Size: 33 9445 BamHI G'GATC_C Cuts at: 4036 6808 7175 4036 Size: 2772 367 6339 Fragments arranged by size: 6339 2772 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6799 8254 618 Size: 325 217 627 5012 1455 1842 Fragments arranged by size: 5012 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 5254 6133 6797 1391 Size: 3863 879 664 4072 Fragments arranged by size: 4072 3863 879 664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 2665 2691 4461 4890 6453 6655 Size: 971 660 106 26 1770 429 1563 202 Cuts at: 6655 928 Size: 3751 Fragments arranged by size: 3751 1770 1563 971 660 429 202 106 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5945 5979 7669 7703 1903 Size: 34 4008 34 1690 34 3678 Fragments arranged by size: 4008 3678 1690 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2197 4333 7370 8580 2197 Size: 2136 3037 1210 3095 Fragments arranged by size: 3095 3037 2136 1210 BclI T'GATC_A Cuts at: 1548 3391 1548 Size: 1843 7635 BfrBI ATG'CAT Cuts at: 2160 3221 2160 Size: 1061 8417 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6701 8089 136 Size: 107 122 71 631 605 5029 1388 1525 Fragments arranged by size: 5029 1525 1388 631 605 122 107 71 BglII A'GATC_T Cuts at: 3904 9473 3904 Size: 5569 3909 BlpI GC'TnA_GC Cuts at: 3496 5368 6895 3496 Size: 1872 1527 6079 Fragments arranged by size: 6079 1872 1527 Bme1580I G_kGCm'C Cuts at: 1790 6247 7539 8695 1790 Size: 4457 1292 1156 2573 Fragments arranged by size: 4457 2573 1292 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 7209 8167 442 Size: 416 53 83 399 24 5792 958 1753 Fragments arranged by size: 5792 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3138 1056 Size: 2082 7396 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4032 4032 Size: 9478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 4062 8138 1239 Size: 1256 1567 4076 2579 Fragments arranged by size: 4076 2579 1567 1256 Bpu10I CC'TnA_GC Cuts at: 3174 3174 Size: 9478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4730 5132 5660 5825 6161 7603 8468 8766 4730 Size: 402 528 165 336 1442 865 298 5442 Fragments arranged by size: 5442 1442 865 528 402 336 298 165 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5258 8141 882 Size: 617 3759 2883 2219 Fragments arranged by size: 3759 2883 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7269 493 Size: 711 6065 2702 Fragments arranged by size: 6065 2702 711 BsaBI GATnn'nnATC Cuts at: 2208 2433 2901 9472 2208 Size: 225 468 6571 2214 Fragments arranged by size: 6571 2214 468 225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7285 7667 8302 275 Size: 53 83 186 936 5752 382 635 1451 Fragments arranged by size: 5752 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7911 8309 8583 8730 9141 7911 Size: 398 274 147 411 8248 Fragments arranged by size: 8248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4686 4716 6237 6267 6507 Size: 30 165 30 3283 30 1521 30 240 Cuts at: 6507 6537 1178 Size: 30 4119 Fragments arranged by size: 4119 3283 1521 240 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2166 4005 4727 5862 1186 Size: 980 1839 722 1135 4802 Fragments arranged by size: 4802 1839 1135 980 722 BseYI C'CCAG_C Cuts at: 2195 2451 5253 8681 9177 2195 Size: 256 2802 3428 496 2496 Fragments arranged by size: 3428 2802 2496 496 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8905 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6928 7689 8717 1540 Size: 275 186 4927 761 1028 2301 Fragments arranged by size: 4927 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2730 5572 6133 6247 7539 7624 8695 2730 Size: 2842 561 114 1292 85 1071 3513 Fragments arranged by size: 3513 2842 1292 1071 561 114 85 BsmI GAATG_Cn' Cuts at: 2162 3047 4338 6478 6989 7082 2162 Size: 885 1291 2140 511 93 4558 Fragments arranged by size: 4558 2140 1291 885 511 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2696 4248 2696 Size: 1552 7926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2730 5254 5572 6133 6247 6797 7539 Size: 399 940 2524 318 561 114 550 742 Cuts at: 7539 7624 8695 1391 Size: 85 1071 2174 Fragments arranged by size: 2524 2174 1071 940 742 561 550 399 318 114 85 BspEI T'CCGG_A Cuts at: 9141 9141 Size: 9478 BspHI T'CATG_A Cuts at: 7367 9097 7367 Size: 1730 7748 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2266 6619 6833 844 Size: 1422 4353 214 3489 Fragments arranged by size: 4353 3489 1422 214 BsrBI CCG'CTC Cuts at: 1394 1823 7365 1394 Size: 429 5542 3507 Fragments arranged by size: 5542 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6321 6398 7973 8147 65 Size: 1183 5073 77 1575 174 1396 Fragments arranged by size: 5073 1575 1396 1183 174 77 BsrFI r'CCGG_y Cuts at: 1700 1810 8122 8309 1700 Size: 110 6312 187 2869 Fragments arranged by size: 6312 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2422 4480 4520 6337 96 Size: 2326 2058 40 1817 3237 Fragments arranged by size: 3237 2326 2058 1817 40 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9478 BssSI C'ACGA_G Cuts at: 4866 5170 7538 8550 4866 Size: 304 2368 1012 5794 Fragments arranged by size: 5794 2368 1012 304 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9478 BstBI TT'CG_AA Cuts at: 5965 8282 5965 Size: 2317 7161 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9478 BstXI CCAn_nnnn'nTGG Cuts at: 2722 2788 2722 Size: 66 9412 BstYI r'GATC_y Cuts at: 1972 2711 3904 4036 6272 6619 6808 7175 7560 Size: 739 1193 132 2236 347 189 367 385 Cuts at: 7560 7577 9029 9115 9127 9473 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2236 1977 1452 1193 739 385 367 347 346 189 132 86 17 12 BstZ17I GTA'TAC Cuts at: 4439 4439 Size: 9478 Bsu36I CC'TnA_GG Cuts at: 3794 3794 Size: 9478 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8926 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8739 Fragments arranged by size: 8739 683 56 BtsI GCAGTG_nn' Cuts at: 3318 6994 7788 7808 3318 Size: 3676 794 20 4988 Fragments arranged by size: 4988 3676 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9443 DraI TTT'AAA Cuts at: 4614 5752 6718 6782 4614 Size: 1138 966 64 7310 Fragments arranged by size: 7310 1138 966 64 DraIII CAC_nnn'GTG Cuts at: 2134 2134 Size: 9478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8485 809 Size: 507 7169 1802 Fragments arranged by size: 7169 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2466 2601 3142 Size: 54 1425 50 275 18 636 135 541 Cuts at: 3142 6925 7814 9443 8 Size: 3783 889 1629 43 Fragments arranged by size: 3783 1629 1425 889 636 541 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6925 1537 Size: 275 5113 4090 Fragments arranged by size: 5113 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2170 3191 4656 5283 5604 5919 6295 7408 Size: 817 1021 1465 627 321 315 376 1113 Cuts at: 7408 1353 Size: 3423 Fragments arranged by size: 3423 1465 1113 1021 817 627 376 321 315 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3413 8065 8439 8585 387 Size: 1064 138 1824 4652 374 146 1280 Fragments arranged by size: 4652 1824 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 6131 6131 Size: 9478 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 3380 4062 4417 4680 4877 4928 Size: 393 863 885 682 355 263 197 51 Cuts at: 4928 4973 5873 6141 6384 6447 6618 7541 8138 Size: 45 900 268 243 63 171 923 597 Cuts at: 8138 8925 9193 1239 Size: 787 268 1524 Fragments arranged by size: 1524 923 900 885 863 787 682 597 393 355 268 268 263 243 197 171 63 51 45 EcoNI CCTnn'n_nnAGG Cuts at: 4712 4712 Size: 9478 EcoRI G'AATT_C Cuts at: 6787 7165 6787 Size: 378 9100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2336 4652 5284 5903 2336 Size: 2316 632 619 5911 Fragments arranged by size: 5911 2316 632 619 FspI TGC'GCA Cuts at: 6182 7984 6182 Size: 1802 7676 HaeII r_GCGC'y Cuts at: 1678 8625 1678 Size: 6947 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2954 2986 3872 3904 4187 Size: 32 135 32 1382 32 886 32 283 Cuts at: 4187 4219 8119 8151 8193 8225 1373 Size: 32 3900 32 42 32 2626 Fragments arranged by size: 3900 2626 1382 886 283 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2029 2769 6822 7068 669 Size: 988 281 91 740 4053 246 3079 Fragments arranged by size: 4053 3079 988 740 281 246 91 HindIII A'AGCT_T Cuts at: 748 3414 5702 5860 5885 6155 748 Size: 2666 2288 158 25 270 4071 Fragments arranged by size: 4071 2666 2288 270 158 25 HpaI GTT'AAC Cuts at: 2029 2769 7068 2029 Size: 740 4299 4439 Fragments arranged by size: 4439 4299 740 KpnI G_GTAC'C Cuts at: 6803 6803 Size: 9478 MfeI C'AATT_G Cuts at: 5394 6404 7077 5394 Size: 1010 673 7795 Fragments arranged by size: 7795 1010 673 MluI A'CGCG_T Cuts at: 9354 9354 Size: 9478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 4377 8592 8776 1305 Size: 1293 1779 4215 184 2007 Fragments arranged by size: 4215 2007 1779 1293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2468 2603 10 Size: 54 1425 343 636 135 6885 Fragments arranged by size: 6885 1425 636 343 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 3834 4516 7436 7795 7954 518 Size: 648 2668 682 2920 359 159 2042 Fragments arranged by size: 2920 2668 2042 682 648 359 159 MspA1I CmG'CkG Cuts at: 1606 6278 6376 6894 7571 8719 8964 1606 Size: 4672 98 518 677 1148 245 2120 Fragments arranged by size: 4672 2120 1148 677 518 245 98 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8926 NdeI CA'TA_TG Cuts at: 387 6208 6354 387 Size: 5821 146 3511 Fragments arranged by size: 5821 3511 146 NheI G'CTAG_C Cuts at: 1052 3134 1052 Size: 2082 7396 NotI GC'GGCC_GC Cuts at: 6925 6925 Size: 9478 NsiI A_TGCA'T Cuts at: 2162 3223 2162 Size: 1061 8417 NspI r_CATG'y Cuts at: 1132 1229 2096 2798 3353 3839 6460 6836 8381 Size: 97 867 702 555 486 2621 376 1545 Cuts at: 8381 1132 Size: 2229 Fragments arranged by size: 2621 2229 1545 867 702 555 486 376 97 PasI CC'CwG_GG Cuts at: 1348 6654 1348 Size: 5306 4172 PciI A'CATG_T Cuts at: 2794 3349 8377 2794 Size: 555 5028 3895 Fragments arranged by size: 5028 3895 555 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 1108 Size: 1029 8449 PfoI T'CCnGG_A Cuts at: 5112 5112 Size: 9478 PmeI GTTT'AAAC Cuts at: 6782 6782 Size: 9478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2712 2744 7547 7579 9060 9092 2712 Size: 32 4803 32 1481 32 3098 Fragments arranged by size: 4803 3098 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 6445 1613 Size: 4832 4646 PsiI TTA'TAA Cuts at: 7048 7048 Size: 9478 PstI C_TGCA'G Cuts at: 830 2170 2335 2544 2590 4054 4785 5544 6830 Size: 1340 165 209 46 1464 731 759 1286 Cuts at: 6830 830 Size: 3478 Fragments arranged by size: 3478 1464 1340 1286 759 731 209 165 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9478 SacI G_AGCT'C Cuts at: 6133 6133 Size: 9478 SalI G'TCGA_C Cuts at: 1936 6820 1936 Size: 4884 4594 SapI GCTCTTCn'nnn_ Cuts at: 5919 5919 Size: 9478 SbfI CC_TGCA'GG Cuts at: 6830 6830 Size: 9478 ScaI AGT'ACT Cuts at: 2857 7726 9438 2857 Size: 4869 1712 2897 Fragments arranged by size: 4869 2897 1712 SexAI A'CCwGG_T Cuts at: 2130 2130 Size: 9478 SfcI C'TryA_G Cuts at: 826 1045 2166 2188 2331 2540 2586 3541 4050 Size: 219 1121 22 143 209 46 955 509 Cuts at: 4050 4781 5540 6216 6826 7961 8642 8833 826 Size: 731 759 676 610 1135 681 191 1471 Fragments arranged by size: 1471 1135 1121 955 759 731 681 676 610 509 219 209 191 143 46 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9478 SmaI CCC'GGG Cuts at: 1872 6805 1872 Size: 4933 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2087 4709 5147 5639 5804 6140 Size: 197 926 144 2622 438 492 165 336 Cuts at: 6140 7582 8483 8745 820 Size: 1442 901 262 1553 Fragments arranged by size: 2622 1553 1442 926 901 492 438 336 262 197 165 144 SnaBI TAC'GTA Cuts at: 493 7269 493 Size: 6776 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9478 SphI G_CATG'C Cuts at: 1132 2096 6836 1132 Size: 964 4740 3774 Fragments arranged by size: 4740 3774 964 SspI AAT'ATT Cuts at: 5 52 6349 7402 5 Size: 47 6297 1053 2081 Fragments arranged by size: 6297 2081 1053 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9478 StyI C'CwwG_G Cuts at: 513 1065 2446 3014 3965 4950 6917 513 Size: 552 1381 568 951 985 1967 3074 Fragments arranged by size: 3074 1967 1381 985 951 568 552 SwaI ATTT'AAAT Cuts at: 5752 5752 Size: 9478 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7505 7522 7675 7860 8305 325 Size: 1255 5925 17 153 185 445 1498 Fragments arranged by size: 5925 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2422 2855 3473 Size: 274 80 33 51 1443 445 433 618 Cuts at: 3473 3489 3692 3838 3977 4480 4520 6071 6337 Size: 16 203 146 139 503 40 1551 266 Cuts at: 6337 7724 9436 96 Size: 1387 1712 138 Fragments arranged by size: 1712 1551 1443 1387 618 503 445 433 274 266 203 146 139 138 80 51 40 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 9438 9470 6237 Size: 32 3169 32 6245 Fragments arranged by size: 6245 3169 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9478 XbaI T'CTAG_A Cuts at: 5911 6644 6814 5911 Size: 733 170 8575 Fragments arranged by size: 8575 733 170 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9478 XmaI C'CCGG_G Cuts at: 1870 6803 1870 Size: 4933 4545 XmnI GAAnn'nnTTC Cuts at: 2436 4116 5727 5886 6348 7607 2436 Size: 1680 1611 159 462 1259 4307 Fragments arranged by size: 4307 1680 1611 1259 462 159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7286 276 Size: 53 83 186 936 5752 2468 Fragments arranged by size: 5752 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI XcmI