(Circular) (Six-base) MAPSORT of: pFN21AA0185.seq Check: 1629 from: 1 to: 10318 pFN21AA0185 10318 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2371 4049 4906 6900 2371 Size: 1678 857 1994 5789 Fragments arranged by size: 5789 1994 1678 857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7446 8128 278 Size: 53 83 186 936 5910 682 2468 Fragments arranged by size: 5910 2468 936 682 186 83 53 AccI GT'mk_AC Cuts at: 1937 7661 1937 Size: 5724 4594 Acc65I G'GTAC_C Cuts at: 2579 3442 7639 2579 Size: 863 4197 5258 Fragments arranged by size: 5258 4197 863 AclI AA'CG_TT Cuts at: 1563 8445 8818 1563 Size: 6882 373 3063 Fragments arranged by size: 6882 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 2423 2576 3182 3561 5270 5594 6141 Size: 504 287 153 606 379 1709 324 547 Cuts at: 6141 6591 6940 7178 8381 9765 10033 1632 Size: 450 349 238 1203 1384 268 1917 Fragments arranged by size: 1917 1709 1384 1203 606 547 504 450 379 349 324 287 268 238 153 AfeI AGC'GCT Cuts at: 7514 7514 Size: 10318 AflII C'TTAA_G Cuts at: 820 1017 6941 820 Size: 197 5924 4197 Fragments arranged by size: 5924 4197 197 AflIII A'CryG_T Cuts at: 1203 6507 6981 7011 9217 10194 1203 Size: 5304 474 30 2206 977 1327 Fragments arranged by size: 5304 2206 1327 977 474 30 AgeI A'CCGG_T Cuts at: 9149 9149 Size: 10318 AhdI GACnn_n'nnGTC Cuts at: 2852 4928 9047 2852 Size: 2076 4119 4123 Fragments arranged by size: 4123 4119 2076 AleI CACnn'nnGTG Cuts at: 1166 2921 5793 1166 Size: 1755 2872 5691 Fragments arranged by size: 5691 2872 1755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10318 AlwNI CAG_nnn'CTG Cuts at: 2719 3706 4459 4522 5129 5611 5983 6275 8061 Size: 987 753 63 607 482 372 292 1786 Cuts at: 8061 9633 2719 Size: 1572 3404 Fragments arranged by size: 3404 1786 1572 987 753 607 482 372 292 63 ApaI G_GGCC'C Cuts at: 6940 6940 Size: 10318 ApaLI G'TGCA_C Cuts at: 3026 4721 8375 9531 3026 Size: 1695 3654 1156 3813 Fragments arranged by size: 3813 3654 1695 1156 ApoI r'AATT_y Cuts at: 1493 4295 5535 7121 7627 7853 8005 10114 1493 Size: 2802 1240 1586 506 226 152 2109 1697 Fragments arranged by size: 2802 2109 1697 1586 1240 506 226 152 AseI AT'TA_AT Cuts at: 160 1032 8872 160 Size: 872 7840 1606 Fragments arranged by size: 7840 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10318 AvaI C'yCGr_G Cuts at: 1870 1943 2031 5274 7643 1870 Size: 73 88 3243 2369 4545 Fragments arranged by size: 4545 3243 2369 88 73 BamHI G'GATC_C Cuts at: 2123 2944 4159 5062 6793 7648 8015 2123 Size: 821 1215 903 1731 855 367 4426 Fragments arranged by size: 4426 1731 1215 903 855 821 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3442 3709 5228 7199 Size: 325 217 627 792 863 267 1519 1971 Cuts at: 7199 7639 9094 618 Size: 440 1455 1842 Fragments arranged by size: 1971 1842 1519 1455 863 792 627 440 325 267 217 BanII G_rGCy'C Cuts at: 1391 3726 6674 6848 6940 7258 7604 7637 1391 Size: 2335 2948 174 92 318 346 33 4072 Fragments arranged by size: 4072 2948 2335 346 318 174 92 33 BbeI G_GCGC'C Cuts at: 7203 7203 Size: 10318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2604 3211 3947 5267 5306 5829 6597 Size: 971 705 607 736 1320 39 523 768 Cuts at: 6597 928 Size: 4649 Fragments arranged by size: 4649 1320 971 768 736 705 607 523 39 BbvCI CC'TCA_GC Cuts at: 2547 4769 6276 2547 Size: 2222 1507 6589 Fragments arranged by size: 6589 2222 1507 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3066 3100 6535 6569 8509 8543 1903 Size: 34 1129 34 3435 34 1940 34 3678 Fragments arranged by size: 3678 3435 1940 1129 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2596 5201 8210 9420 2596 Size: 2605 3009 1210 3494 Fragments arranged by size: 3494 3009 2605 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2325 5615 8929 Size: 107 122 71 631 605 653 3290 3314 Cuts at: 8929 136 Size: 1525 Fragments arranged by size: 3314 3290 1525 653 631 605 122 107 71 BglII A'GATC_T Cuts at: 10313 10313 Size: 10318 BlpI GC'TnA_GC Cuts at: 3191 6842 7367 7735 3191 Size: 3651 525 368 5774 Fragments arranged by size: 5774 3651 525 368 Bme1580I G_kGCm'C Cuts at: 1790 3030 3380 3714 4614 4725 5231 6940 8379 Size: 1240 350 334 900 111 506 1709 1439 Cuts at: 8379 9535 1790 Size: 1156 2573 Fragments arranged by size: 2573 1709 1439 1240 1156 900 506 350 334 111 BmgBI CAC'GTC Cuts at: 1318 6217 6508 1318 Size: 4899 291 5128 Fragments arranged by size: 5128 4899 291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6213 8049 9007 Size: 416 53 83 399 24 4796 1836 958 Cuts at: 9007 442 Size: 1753 Fragments arranged by size: 4796 1836 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10318 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5248 5968 5248 Size: 720 9598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 3977 4037 4481 5399 7025 7280 7601 Size: 1067 1671 60 444 918 1626 255 321 Cuts at: 7601 8978 1239 Size: 1377 2579 Fragments arranged by size: 2579 1671 1626 1377 1067 918 444 321 255 60 Bpu10I CC'TnA_GC Cuts at: 2547 2862 3032 4769 6276 2547 Size: 315 170 1737 1507 6589 Fragments arranged by size: 6589 1737 1507 315 170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4928 5522 5705 7334 8443 9308 9606 4928 Size: 594 183 1629 1109 865 298 5640 Fragments arranged by size: 5640 1629 1109 865 594 298 183 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4141 6279 8981 882 Size: 617 2642 2138 2702 2219 Fragments arranged by size: 2702 2642 2219 2138 617 BsaAI yAC'GTr Cuts at: 493 1204 8109 493 Size: 711 6905 2702 Fragments arranged by size: 6905 2702 711 BsaBI GATnn'nnATC Cuts at: 10312 10312 Size: 10318 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3064 5813 7200 7443 Size: 53 83 186 936 1531 2749 1387 243 Cuts at: 7443 8125 8507 9142 275 Size: 682 382 635 1451 Fragments arranged by size: 2749 1531 1451 1387 936 682 635 382 243 186 83 53 BsaWI w'CCGG_w Cuts at: 3481 7149 8751 9149 9423 9570 9981 3481 Size: 3668 1602 398 274 147 411 3818 Fragments arranged by size: 3818 3668 1602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2745 2775 3476 3506 5087 Size: 30 165 30 1342 30 701 30 1581 Cuts at: 5087 5117 5789 5819 6998 7028 1178 Size: 30 672 30 1179 30 4468 Fragments arranged by size: 4468 1581 1342 1179 701 672 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2049 2769 4382 4430 4433 4478 6272 6293 Size: 863 720 1613 48 3 45 1794 21 Cuts at: 6293 6362 6500 6545 6698 6794 6977 7231 1186 Size: 69 138 45 153 96 183 254 4273 Fragments arranged by size: 4273 1794 1613 863 720 254 183 153 138 96 69 48 45 45 21 3 BseYI C'CCAG_C Cuts at: 3143 6075 6362 7308 7324 9521 10017 3143 Size: 2932 287 946 16 2197 496 3444 Fragments arranged by size: 3444 2932 2197 946 496 287 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3719 4583 4820 7079 7577 1163 Size: 573 1983 864 237 2259 498 3904 Fragments arranged by size: 3904 2259 1983 864 573 498 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7768 8529 9557 1540 Size: 275 186 5767 761 1028 2301 Fragments arranged by size: 5767 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3030 3726 4725 6674 6848 7373 7604 8379 8464 Size: 696 999 1949 174 525 231 775 85 Cuts at: 8464 9535 3030 Size: 1071 3813 Fragments arranged by size: 3813 1949 1071 999 775 696 525 231 174 85 BsmI GAATG_Cn' Cuts at: 2259 2448 4197 7829 7922 2259 Size: 189 1749 3632 93 4655 Fragments arranged by size: 4655 3632 1749 189 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4835 5752 5804 5912 6536 4835 Size: 917 52 108 624 8617 Fragments arranged by size: 8617 917 624 108 52 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3030 3380 3714 3726 4614 4725 5231 Size: 399 1240 350 334 12 888 111 506 Cuts at: 5231 6674 6848 6940 7258 7373 7604 7637 8379 Size: 1443 174 92 318 115 231 33 742 Cuts at: 8379 8464 9535 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1443 1240 1071 888 742 506 399 350 334 318 231 174 115 111 92 85 33 12 BspEI T'CCGG_A Cuts at: 3481 9981 3481 Size: 6500 3818 BspHI T'CATG_A Cuts at: 3346 4284 8207 9937 3346 Size: 938 3923 1730 3727 Fragments arranged by size: 3923 3727 1730 938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 3614 4049 4906 6900 7673 844 Size: 1527 1243 435 857 1994 773 3489 Fragments arranged by size: 3489 1994 1527 1243 857 773 435 BsrBI CCG'CTC Cuts at: 1394 1823 7339 7462 8205 1394 Size: 429 5516 123 743 3507 Fragments arranged by size: 5516 3507 743 429 123 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 4824 7293 8813 8987 65 Size: 1183 1551 2025 2469 1520 174 1396 Fragments arranged by size: 2469 2025 1551 1520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5447 6473 8962 9149 1700 Size: 110 3183 454 1026 2489 187 2869 Fragments arranged by size: 3183 2869 2489 1026 454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10318 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10318 BssSI C'ACGA_G Cuts at: 8378 9390 8378 Size: 1012 9306 BstAPI GCAn_nnn'nTGC Cuts at: 794 3000 4381 4477 794 Size: 2206 1381 96 6635 Fragments arranged by size: 6635 2206 1381 96 BstBI TT'CG_AA Cuts at: 5341 9122 5341 Size: 3781 6537 BstEII G'GTnAC_C Cuts at: 1184 4351 1184 Size: 3167 7151 BstXI CCAn_nnnn'nTGG Cuts at: 3095 3457 7133 7579 3095 Size: 362 3676 446 5834 Fragments arranged by size: 5834 3676 446 362 BstYI r'GATC_y Cuts at: 1972 2049 2123 2944 4159 5062 5938 6793 7648 Size: 77 74 821 1215 903 876 855 855 Cuts at: 7648 8015 8400 8417 9869 9955 9967 10313 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1215 903 876 855 855 821 385 367 346 86 77 74 17 12 Bsu36I CC'TnA_GG Cuts at: 3228 4252 5612 3228 Size: 1024 1360 7934 Fragments arranged by size: 7934 1360 1024 BtgI C'CryG_G Cuts at: 513 1065 2004 2602 5421 5703 6816 6900 6924 Size: 552 939 598 2819 282 1113 84 24 Cuts at: 6924 513 Size: 3907 Fragments arranged by size: 3907 2819 1113 939 598 552 282 84 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4628 487 Size: 683 56 3402 6177 Fragments arranged by size: 6177 3402 683 56 BtsI GCAGTG_nn' Cuts at: 2651 2988 3108 3372 4391 5268 5391 5865 7834 Size: 337 120 264 1019 877 123 474 1969 Cuts at: 7834 8628 8648 2651 Size: 794 20 4321 Fragments arranged by size: 4321 1969 1019 877 794 474 337 264 123 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10318 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5608 5643 529 Size: 35 5044 35 5204 Fragments arranged by size: 5204 5044 35 35 DraI TTT'AAA Cuts at: 7622 7622 Size: 10318 DraIII CAC_nnn'GTG Cuts at: 6017 6019 7243 6017 Size: 2 1224 9092 Fragments arranged by size: 9092 1224 2 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 9325 809 Size: 507 3818 4191 1802 Fragments arranged by size: 4191 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2796 3769 4153 Size: 54 1425 50 275 18 966 973 384 Cuts at: 4153 4524 5366 5386 5449 5602 5700 7765 8654 Size: 371 842 20 63 153 98 2065 889 Cuts at: 8654 10283 8 Size: 1629 43 Fragments arranged by size: 2065 1629 1425 973 966 889 842 384 371 275 153 98 63 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 7765 1537 Size: 275 5953 4090 Fragments arranged by size: 5953 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2489 4208 6534 8248 1353 Size: 759 377 1719 2326 1714 3423 Fragments arranged by size: 3423 2326 1719 1714 759 377 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8905 9279 9425 387 Size: 1064 138 7316 374 146 1280 Fragments arranged by size: 7316 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3724 6672 6846 7602 3724 Size: 2948 174 756 6440 Fragments arranged by size: 6440 2948 756 174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2306 2423 2576 3182 3561 3977 Size: 393 504 170 117 153 606 379 416 Cuts at: 3977 4037 4481 5270 5399 5594 6141 6591 6940 Size: 60 444 789 129 195 547 450 349 Cuts at: 6940 7025 7178 7280 7601 8381 8978 9765 10033 Size: 85 153 102 321 780 597 787 268 Cuts at: 10033 1239 Size: 1524 Fragments arranged by size: 1524 789 787 780 606 597 547 504 450 444 416 393 379 349 321 268 195 170 153 153 129 117 102 85 60 EcoNI CCTnn'n_nnAGG Cuts at: 2246 4736 6482 7037 2246 Size: 2490 1746 555 5527 Fragments arranged by size: 5527 2490 1746 555 EcoO109I rG'GnC_Cy Cuts at: 2407 2551 2988 3436 5608 5672 6145 6936 6937 Size: 144 437 448 2172 64 473 791 1 Cuts at: 6937 7576 2407 Size: 639 5149 Fragments arranged by size: 5149 2172 791 639 473 448 437 144 64 1 EcoRI G'AATT_C Cuts at: 5535 7627 8005 5535 Size: 2092 378 7848 Fragments arranged by size: 7848 2092 378 EcoRV GAT'ATC Cuts at: 3344 3344 Size: 10318 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3235 4147 5668 5996 6260 7522 3235 Size: 912 1521 328 264 1262 6031 Fragments arranged by size: 6031 1521 1262 912 328 264 FseI GG_CCGG'CC Cuts at: 5451 5451 Size: 10318 FspI TGC'GCA Cuts at: 4481 8824 4481 Size: 4343 5975 HaeII r_GCGC'y Cuts at: 1678 6590 6788 7203 7516 9465 1678 Size: 4912 198 415 313 1949 2531 Fragments arranged by size: 4912 2531 1949 415 313 198 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2838 2870 3054 3086 3463 Size: 32 135 32 1266 32 184 32 377 Cuts at: 3463 3495 4649 4681 4914 4946 5789 5821 5841 Size: 32 1154 32 233 32 843 32 20 Cuts at: 5841 5873 7382 7414 8959 8991 9033 9065 1373 Size: 32 1509 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1545 1509 1266 1154 843 377 233 184 135 42 32 32 32 32 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 2530 2929 7662 7908 669 Size: 988 281 592 399 4733 246 3079 Fragments arranged by size: 4733 3079 988 592 399 281 246 HindIII A'AGCT_T Cuts at: 748 2023 2067 5681 748 Size: 1275 44 3614 5385 Fragments arranged by size: 5385 3614 1275 44 HpaI GTT'AAC Cuts at: 7908 7908 Size: 10318 KasI G'GCGC_C Cuts at: 7199 7199 Size: 10318 KpnI G_GTAC'C Cuts at: 2583 3446 7643 2583 Size: 863 4197 5258 Fragments arranged by size: 5258 4197 863 MfeI C'AATT_G Cuts at: 7917 7917 Size: 10318 MluI A'CGCG_T Cuts at: 10194 10194 Size: 10318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3993 4383 4554 4695 6720 9432 9616 1305 Size: 2688 390 171 141 2025 2712 184 2007 Fragments arranged by size: 2712 2688 2025 2007 390 184 171 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 3771 4155 5388 5604 5702 Size: 54 1425 343 1939 384 1233 216 98 Cuts at: 5702 10 Size: 4626 Fragments arranged by size: 4626 1939 1425 1233 384 343 216 98 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3470 5793 7287 8276 8635 8794 Size: 648 1755 549 2323 1494 989 359 159 Cuts at: 8794 518 Size: 2042 Fragments arranged by size: 2323 2042 1755 1494 989 648 549 359 159 MspA1I CmG'CkG Cuts at: 1606 4378 6601 6818 7324 7734 8411 9559 9804 Size: 2772 2223 217 506 410 677 1148 245 Cuts at: 9804 1606 Size: 2120 Fragments arranged by size: 2772 2223 2120 1148 677 506 410 245 217 NaeI GCC'GGC Cuts at: 4995 5449 6475 4995 Size: 454 1026 8838 Fragments arranged by size: 8838 1026 454 NarI GG'CG_CC Cuts at: 7200 7200 Size: 10318 NcoI C'CATG_G Cuts at: 513 1065 2004 2602 5421 5703 513 Size: 552 939 598 2819 282 5128 Fragments arranged by size: 5128 2819 939 598 552 282 NdeI CA'TA_TG Cuts at: 387 3400 387 Size: 3013 7305 NgoMIV G'CCGG_C Cuts at: 4993 5447 6473 4993 Size: 454 1026 8838 Fragments arranged by size: 8838 1026 454 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10318 NotI GC'GGCC_GC Cuts at: 7765 7765 Size: 10318 NspI r_CATG'y Cuts at: 1132 1229 2569 7015 7286 7676 9221 1132 Size: 97 1340 4446 271 390 1545 2229 Fragments arranged by size: 4446 2229 1545 1340 390 271 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10318 PciI A'CATG_T Cuts at: 7011 9217 7011 Size: 2206 8112 PflMI CCAn_nnn'nTGG Cuts at: 1108 4522 4744 6867 1108 Size: 3414 222 2123 4559 Fragments arranged by size: 4559 3414 2123 222 PfoI T'CCnGG_A Cuts at: 4186 5349 5958 7125 7446 4186 Size: 1163 609 1167 321 7058 Fragments arranged by size: 7058 1167 1163 609 321 PmeI GTTT'AAAC Cuts at: 7622 7622 Size: 10318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8387 8419 9900 9932 8387 Size: 32 1481 32 8773 Fragments arranged by size: 8773 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 5672 6145 2407 Size: 3265 473 6580 Fragments arranged by size: 6580 3265 473 PshAI GACnn'nnGTC Cuts at: 1613 4410 7040 1613 Size: 2797 2630 4891 Fragments arranged by size: 4891 2797 2630 PsiI TTA'TAA Cuts at: 6105 7888 6105 Size: 1783 8535 PspOMI G'GGCC_C Cuts at: 6936 6936 Size: 10318 PstI C_TGCA'G Cuts at: 830 3636 3874 4473 5872 6600 6876 6897 7231 Size: 2806 238 599 1399 728 276 21 334 Cuts at: 7231 7251 7670 830 Size: 20 419 3478 Fragments arranged by size: 3478 2806 1399 728 599 419 334 276 238 21 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10318 PvuII CAG'CTG Cuts at: 6601 7324 6601 Size: 723 9595 SacI G_AGCT'C Cuts at: 3726 6674 6848 7604 3726 Size: 2948 174 756 6440 Fragments arranged by size: 6440 2948 756 174 SacII CC_GC'GG Cuts at: 6819 6819 Size: 10318 SalI G'TCGA_C Cuts at: 1936 7660 1936 Size: 5724 4594 SapI GCTCTTCn'nnn_ Cuts at: 4208 4208 Size: 10318 SbfI CC_TGCA'GG Cuts at: 3874 6897 7670 3874 Size: 3023 773 6522 Fragments arranged by size: 6522 3023 773 ScaI AGT'ACT Cuts at: 3730 8566 10278 3730 Size: 4836 1712 3770 Fragments arranged by size: 4836 3770 1712 SexAI A'CCwGG_T Cuts at: 4617 6171 4617 Size: 1554 8764 SfcI C'TryA_G Cuts at: 826 1045 3053 3274 3632 3870 4469 5076 5868 Size: 219 2008 221 358 238 599 607 792 Cuts at: 5868 6596 6872 6893 7227 7247 7666 8801 9482 Size: 728 276 21 334 20 419 1135 681 Cuts at: 9482 9673 826 Size: 191 1471 Fragments arranged by size: 2008 1471 1135 792 728 681 607 599 419 358 334 276 238 221 219 191 21 20 SfiI GGCCn_nnn'nGGCC Cuts at: 5615 5615 Size: 10318 SfoI GGC'GCC Cuts at: 7201 7201 Size: 10318 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10318 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10318 SmaI CCC'GGG Cuts at: 1872 7645 1872 Size: 5773 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4943 5501 5684 6941 7313 8422 Size: 197 926 3000 558 183 1257 372 1109 Cuts at: 8422 9323 9585 820 Size: 901 262 1553 Fragments arranged by size: 3000 1553 1257 1109 926 901 558 372 262 197 183 SnaBI TAC'GTA Cuts at: 493 8109 493 Size: 7616 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10318 SphI G_CATG'C Cuts at: 1132 2569 7676 1132 Size: 1437 5107 3774 Fragments arranged by size: 5107 3774 1437 SspI AAT'ATT Cuts at: 5 52 8242 5 Size: 47 8190 2081 Fragments arranged by size: 8190 2081 47 StuI AGG'CCT Cuts at: 1517 3233 1517 Size: 1716 8602 StyI C'CwwG_G Cuts at: 513 1065 2004 2602 3094 4131 4833 5421 5703 Size: 552 939 598 492 1037 702 588 282 Cuts at: 5703 6031 6231 6558 7038 7572 7757 513 Size: 328 200 327 480 534 185 3074 Fragments arranged by size: 3074 1037 939 702 598 588 552 534 492 480 328 327 282 200 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6846 6976 7390 8345 8362 8515 8700 Size: 1255 5266 130 414 955 17 153 185 Cuts at: 8700 9145 325 Size: 445 1498 Fragments arranged by size: 5266 1498 1255 955 445 414 185 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 3728 6510 Size: 274 80 33 51 1443 693 1058 2782 Cuts at: 6510 6876 7062 8564 10276 96 Size: 366 186 1502 1712 138 Fragments arranged by size: 2782 1712 1502 1443 1058 693 366 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5087 5119 6071 6103 10278 10310 5087 Size: 32 952 32 4175 32 5095 Fragments arranged by size: 5095 4175 952 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3879 4619 5117 5828 5887 1316 Size: 2563 740 498 711 59 5747 Fragments arranged by size: 5747 2563 740 711 498 59 XbaI T'CTAG_A Cuts at: 5209 7654 5209 Size: 2445 7873 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3952 4831 5113 5710 2011 Size: 1941 879 282 597 6619 Fragments arranged by size: 6619 1941 879 597 282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10318 XmaI C'CCGG_G Cuts at: 1870 7643 1870 Size: 5773 4545 XmnI GAAnn'nnTTC Cuts at: 2069 3218 5303 7163 7502 8447 2069 Size: 1149 2085 1860 339 945 3940 Fragments arranged by size: 3940 2085 1860 1149 945 339 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7444 8126 276 Size: 53 83 186 936 5910 682 2468 Fragments arranged by size: 5910 2468 936 682 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SrfI SwaI