(Circular) (Six-base) MAPSORT of: pFN21AA0164.seq Check: 7915 from: 1 to: 7465 pFN21AA0164 7465 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5275 278 Size: 53 83 186 936 3739 2468 Fragments arranged by size: 3739 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 4808 1937 Size: 1641 1230 4594 Fragments arranged by size: 4594 1641 1230 Acc65I G'GTAC_C Cuts at: 4786 4786 Size: 7465 AclI AA'CG_TT Cuts at: 1563 5592 5965 1563 Size: 4029 373 3063 Fragments arranged by size: 4029 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 3139 3568 3660 3792 3942 4251 5528 Size: 900 607 429 92 132 150 309 1277 Cuts at: 5528 6912 7180 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1277 900 607 429 309 268 150 132 92 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7268 AflIII A'CryG_T Cuts at: 1203 6364 7341 1203 Size: 5161 977 1327 Fragments arranged by size: 5161 1327 977 AgeI A'CCGG_T Cuts at: 6296 6296 Size: 7465 AhdI GACnn_n'nnGTC Cuts at: 6194 6194 Size: 7465 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7465 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7465 AlwNI CAG_nnn'CTG Cuts at: 5208 6780 5208 Size: 1572 5893 ApaLI G'TGCA_C Cuts at: 5522 6678 5522 Size: 1156 6309 ApoI r'AATT_y Cuts at: 1493 2606 3454 3755 4331 4774 5000 5152 7261 Size: 1113 848 301 576 443 226 152 2109 Cuts at: 7261 1493 Size: 1697 Fragments arranged by size: 2109 1697 1113 848 576 443 301 226 152 AseI AT'TA_AT Cuts at: 160 1032 6019 160 Size: 872 4987 1606 Fragments arranged by size: 4987 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7465 AvaI C'yCGr_G Cuts at: 1870 1943 4293 4790 1870 Size: 73 2350 497 4545 Fragments arranged by size: 4545 2350 497 73 BamHI G'GATC_C Cuts at: 4510 4795 5162 4510 Size: 285 367 6813 Fragments arranged by size: 6813 367 285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3658 4786 6241 618 Size: 325 217 627 1871 1128 1455 1842 Fragments arranged by size: 1871 1842 1455 1128 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 4145 4784 1391 Size: 2559 195 639 4072 Fragments arranged by size: 4072 2559 639 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3453 928 Size: 971 1554 4940 Fragments arranged by size: 4940 1554 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5656 5690 1903 Size: 34 3719 34 3678 Fragments arranged by size: 3719 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5357 6567 5357 Size: 1210 6255 BclI T'GATC_A Cuts at: 1548 1548 Size: 7465 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6076 136 Size: 107 122 71 631 605 4404 1525 Fragments arranged by size: 4404 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2081 2387 2405 7460 2081 Size: 306 18 5055 2086 Fragments arranged by size: 5055 2086 306 18 BlpI GC'TnA_GC Cuts at: 4882 4882 Size: 7465 Bme1580I G_kGCm'C Cuts at: 1790 5526 6682 1790 Size: 3736 1156 2573 Fragments arranged by size: 3736 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7465 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 4281 5196 Size: 416 53 83 399 24 1595 1269 915 Cuts at: 5196 6154 442 Size: 958 1753 Fragments arranged by size: 1753 1595 1269 958 915 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7465 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3645 3645 Size: 7465 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4129 6125 1239 Size: 2890 1996 2579 Fragments arranged by size: 2890 2579 1996 Bpu10I CC'TnA_GC Cuts at: 3495 3495 Size: 7465 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3296 5590 6455 6753 3296 Size: 2294 865 298 4008 Fragments arranged by size: 4008 2294 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2121 2145 2421 2490 4483 4706 6128 Size: 617 622 24 276 69 1993 223 1422 Cuts at: 6128 882 Size: 2219 Fragments arranged by size: 2219 1993 1422 622 617 276 223 69 24 BsaAI yAC'GTr Cuts at: 493 1204 5256 493 Size: 711 4052 2702 Fragments arranged by size: 4052 2702 711 BsaBI GATnn'nnATC Cuts at: 2074 2080 4258 7459 2074 Size: 6 2178 3201 2080 Fragments arranged by size: 3201 2178 2080 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5272 5654 6289 275 Size: 53 83 186 936 3739 382 635 1451 Fragments arranged by size: 3739 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2630 3288 5898 6296 6570 6717 7128 2630 Size: 658 2610 398 274 147 411 2967 Fragments arranged by size: 2967 2610 658 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7240 Fragments arranged by size: 7240 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2268 2271 3227 3314 4446 1186 Size: 1082 3 956 87 1132 4205 Fragments arranged by size: 4205 1132 1082 956 87 3 BseYI C'CCAG_C Cuts at: 4442 6668 7164 4442 Size: 2226 496 4743 Fragments arranged by size: 4743 2226 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6892 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4915 5676 6704 1540 Size: 275 186 2914 761 1028 2301 Fragments arranged by size: 2914 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5526 5611 6682 5526 Size: 85 1071 6309 Fragments arranged by size: 6309 1071 85 BsmI GAATG_Cn' Cuts at: 2785 2935 4976 5069 2785 Size: 150 2041 93 5181 Fragments arranged by size: 5181 2041 150 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2200 2200 Size: 7465 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3950 4145 4784 5526 5611 6682 1391 Size: 399 2160 195 639 742 85 1071 2174 Fragments arranged by size: 2174 2160 1071 742 639 399 195 85 BspEI T'CCGG_A Cuts at: 7128 7128 Size: 7465 BspHI T'CATG_A Cuts at: 4669 5354 7084 4669 Size: 685 1730 5050 Fragments arranged by size: 5050 1730 685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3490 3697 4820 844 Size: 2646 207 1123 3489 Fragments arranged by size: 3489 2646 1123 207 BsrBI CCG'CTC Cuts at: 1394 1823 3895 5352 1394 Size: 429 2072 1457 3507 Fragments arranged by size: 3507 2072 1457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3885 5960 6134 65 Size: 1183 2637 2075 174 1396 Fragments arranged by size: 2637 2075 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2394 6109 6296 1700 Size: 110 584 3715 187 2869 Fragments arranged by size: 3715 2869 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7465 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7465 BssSI C'ACGA_G Cuts at: 3567 4389 5525 6537 3567 Size: 822 1136 1012 4495 Fragments arranged by size: 4495 1136 1012 822 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7465 BstBI TT'CG_AA Cuts at: 4405 6269 4405 Size: 1864 5601 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7465 BstXI CCAn_nnnn'nTGG Cuts at: 4688 4688 Size: 7465 BstYI r'GATC_y Cuts at: 1972 2081 2357 2375 2387 2405 2843 4510 4795 Size: 109 276 18 12 18 438 1667 285 Cuts at: 4795 5162 5547 5564 7016 7102 7114 7460 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1667 1452 438 385 367 346 285 276 109 86 18 18 17 12 12 Bsu36I CC'TnA_GG Cuts at: 3989 3989 Size: 7465 BtgI C'CryG_G Cuts at: 513 1065 2004 4716 513 Size: 552 939 2712 3262 Fragments arranged by size: 3262 2712 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6726 Fragments arranged by size: 6726 683 56 BtsI GCAGTG_nn' Cuts at: 4981 5775 5795 4981 Size: 794 20 6651 Fragments arranged by size: 6651 794 20 ClaI AT'CG_AT Cuts at: 1554 2601 1554 Size: 1047 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7430 DraI TTT'AAA Cuts at: 4030 4336 4769 4030 Size: 306 433 6726 Fragments arranged by size: 6726 433 306 DraIII CAC_nnn'GTG Cuts at: 3832 3832 Size: 7465 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6472 809 Size: 507 5156 1802 Fragments arranged by size: 5156 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4912 5801 7430 Size: 54 1425 50 275 18 3082 889 1629 Cuts at: 7430 8 Size: 43 Fragments arranged by size: 3082 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4912 1537 Size: 275 3100 4090 Fragments arranged by size: 4090 3100 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2437 2523 3027 4011 4270 4417 4494 4588 Size: 1084 86 504 984 259 147 77 94 Cuts at: 4588 4704 5395 1353 Size: 116 691 3423 Fragments arranged by size: 3423 1084 984 691 504 259 147 116 94 86 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6052 6426 6572 387 Size: 1064 138 4463 374 146 1280 Fragments arranged by size: 4463 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 3139 3568 3660 3792 3942 4129 Size: 393 900 607 429 92 132 150 187 Cuts at: 4129 4251 5528 6125 6912 7180 1239 Size: 122 1277 597 787 268 1524 Fragments arranged by size: 1524 1277 900 787 607 597 429 393 268 187 150 132 122 92 EcoNI CCTnn'n_nnAGG Cuts at: 2322 2322 Size: 7465 EcoRI G'AATT_C Cuts at: 3755 4774 5152 3755 Size: 1019 378 6068 Fragments arranged by size: 6068 1019 378 EcoRV GAT'ATC Cuts at: 2267 2267 Size: 7465 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3598 4558 4561 3598 Size: 960 3 6502 Fragments arranged by size: 6502 960 3 FspI TGC'GCA Cuts at: 5971 5971 Size: 7465 HaeII r_GCGC'y Cuts at: 1678 4630 6612 1678 Size: 2952 1982 2531 Fragments arranged by size: 2952 2531 1982 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3020 3052 3083 3115 3152 Size: 32 135 32 1448 32 31 32 37 Cuts at: 3152 3184 6106 6138 6180 6212 1373 Size: 32 2922 32 42 32 2626 Fragments arranged by size: 2922 2626 1448 135 42 37 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 4809 5055 669 Size: 988 281 2871 246 3079 Fragments arranged by size: 3079 2871 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7465 HpaI GTT'AAC Cuts at: 5055 5055 Size: 7465 KpnI G_GTAC'C Cuts at: 4790 4790 Size: 7465 MfeI C'AATT_G Cuts at: 5064 5064 Size: 7465 MluI A'CGCG_T Cuts at: 7341 7341 Size: 7465 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2815 6579 6763 1305 Size: 1510 3764 184 2007 Fragments arranged by size: 3764 2007 1510 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5643 Fragments arranged by size: 5643 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 5423 5782 5941 518 Size: 648 1108 3149 359 159 2042 Fragments arranged by size: 3149 2042 1108 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3875 4881 5558 6706 6951 1606 Size: 2269 1006 677 1148 245 2120 Fragments arranged by size: 2269 2120 1148 1006 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4716 513 Size: 552 939 2712 3262 Fragments arranged by size: 3262 2712 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7465 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7465 NotI GC'GGCC_GC Cuts at: 4912 4912 Size: 7465 NruI TCG'CGA Cuts at: 2181 2181 Size: 7465 NspI r_CATG'y Cuts at: 1132 1229 4823 6368 1132 Size: 97 3594 1545 2229 Fragments arranged by size: 3594 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7465 PciI A'CATG_T Cuts at: 6364 6364 Size: 7465 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7465 PfoI T'CCnGG_A Cuts at: 2339 2339 Size: 7465 PmeI GTTT'AAAC Cuts at: 4769 4769 Size: 7465 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5534 5566 7047 7079 5534 Size: 32 1481 32 5920 Fragments arranged by size: 5920 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2482 1613 Size: 869 6596 PsiI TTA'TAA Cuts at: 3381 5035 3381 Size: 1654 5811 PstI C_TGCA'G Cuts at: 830 4817 830 Size: 3987 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7465 PvuII CAG'CTG Cuts at: 3875 3875 Size: 7465 SalI G'TCGA_C Cuts at: 1936 4807 1936 Size: 2871 4594 SapI GCTCTTCn'nnn_ Cuts at: 4417 4417 Size: 7465 SbfI CC_TGCA'GG Cuts at: 4817 4817 Size: 7465 ScaI AGT'ACT Cuts at: 3934 4541 5713 7425 3934 Size: 607 1172 1712 3974 Fragments arranged by size: 3974 1712 1172 607 SfcI C'TryA_G Cuts at: 826 1045 4813 5948 6629 6820 826 Size: 219 3768 1135 681 191 1471 Fragments arranged by size: 3768 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7465 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7465 SmaI CCC'GGG Cuts at: 1872 4792 1872 Size: 2920 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3311 4293 5569 6470 6732 820 Size: 197 926 1368 982 1276 901 262 1553 Fragments arranged by size: 1553 1368 1276 982 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5256 493 Size: 4763 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7465 SphI G_CATG'C Cuts at: 1132 4823 1132 Size: 3691 3774 SspI AAT'ATT Cuts at: 5 52 5389 5 Size: 47 5337 2081 Fragments arranged by size: 5337 2081 47 StuI AGG'CCT Cuts at: 1517 3415 1517 Size: 1898 5567 StyI C'CwwG_G Cuts at: 513 1065 2004 3837 4137 4681 4716 4904 513 Size: 552 939 1833 300 544 35 188 3074 Fragments arranged by size: 3074 1833 939 552 544 300 188 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4507 5492 5509 5662 5847 6292 325 Size: 1255 2927 985 17 153 185 445 1498 Fragments arranged by size: 2927 1498 1255 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2750 3315 3722 Size: 274 80 33 51 1443 773 565 407 Cuts at: 3722 3932 4539 5711 7423 96 Size: 210 607 1172 1712 138 Fragments arranged by size: 1712 1443 1172 773 607 565 407 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3390 3422 3818 3850 7425 7457 3390 Size: 32 396 32 3575 32 3398 Fragments arranged by size: 3575 3398 396 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2325 1316 Size: 1009 6456 XbaI T'CTAG_A Cuts at: 2021 2048 2090 2165 3038 3145 4801 2021 Size: 27 42 75 873 107 1656 4685 Fragments arranged by size: 4685 1656 873 107 75 42 27 XhoI C'TCGA_G Cuts at: 1943 4293 1943 Size: 2350 5115 XmaI C'CCGG_G Cuts at: 1870 4790 1870 Size: 2920 4545 XmnI GAAnn'nnTTC Cuts at: 2131 4541 5594 2131 Size: 2410 1053 4002 Fragments arranged by size: 4002 2410 1053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5273 276 Size: 53 83 186 936 3739 2468 Fragments arranged by size: 3739 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI