Restriction map for
pFN21AA0156
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BbvCI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BspEI
BsrBI BsrDI Bst1107I BstAPI BstZ17I
CelII CpoI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 1151 GGTACC G^GTACC
AcyI 2559 GGCGTC G[GA]^CG[CT]C
Alw44I 1608 GTGCAC G^TGCAC
ApaLI 1608 GTGCAC G^TGCAC
Asp700I 418 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1151 GGTACC G^GTACC
BamHI 1040 GGATCC G^GATCC
BbrPI 502 CACGTG CAC^GTG
BbsI 820 GAAGAC GAAGAC(2/6)
BpuAI 820 GAAGAC GAAGAC(2/6)
BsaHI 2559 GGCGTC G[GA]^CG[CT]C
BsaI 2662 GAGACC GGTCTC(1/5)
BsaWI 2297 ACCGGA [AT]^CCGG[AT]
BsiEI 11 CGGCCG CG[GA][CT]^CG
Bsp1407I 1098 TGTACA T^GTACA
BspDI 949 ATCGAT AT^CGAT
BspHI 1496 TCATGA T^CATGA
BspLU11I 1526 ACATGT A^CATGT
BspMI 2129 ACCTGC ACCTGC(4/8)
BsrFI 1225 GCCGGC [GA]^CCGG[CT]
BsrGI 1098 TGTACA T^GTACA
BssHII 1002 GCGCGC G^CGCGC
BstBI 2034 TTCGAA TT^CGAA
BstEII 2496 GGTCACC G^GT[ACGT]ACC
BstPI 2496 GGTCACC G^GT[ACGT]ACC
BstXI 1030 CCAGACTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1225 GCCGGC [GA]^CCGG[CT]
ClaI 949 ATCGAT AT^CGAT
DraI 2257 TTTAAA TTT^AAA
DraIII 2675 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2730 GACGCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoNI 678 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2496 GGTCACC G^GT[ACGT]ACC
HaeII 2762 AGCGCC [GA]GCGC^[CT]
Hin1I 2559 GGCGTC G[GA]^CG[CT]C
HindIII 1214 AAGCTT A^AGCTT
KpnI 1151 GGTACC GGTAC^C
NaeI 1225 GCCGGC GCC^GGC
NdeI 658 CATATG CA^TATG
NgoMIV 1225 GCCGGC G^CCGGC
NspV 2034 TTCGAA TT^CGAA
PflMI 1523 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 502 CACGTG CAC^GTG
PmlI 502 CACGTG CAC^GTG
PpuMI 2483 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1496 TCATGA T^CATGA
ScaI 572 AGTACT AGT^ACT
SexAI 2676 ACCAGGT A^CC[AT]GGT
SfuI 2034 TTCGAA TT^CGAA
SmaI 238 CCCGGG CCC^GGG
SphI 2141 GCATGC GCATG^C
SspBI 1098 TGTACA T^GTACA
TfiI 2551 GAATC G^A[AT]TC
Van91I 1523 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 238 CCCGGG C^CCGGG
XmaI 238 CCCGGG C^CCGGG
XmnI 418 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 503 ACGTGT A^C[GA][CT]GT
1526 ACATGT
AlwNI 868 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2281 CAGGCACTG
ApaI 55 GGGCCC GGGCC^C
1993 GGGCCC
BalI 567 TGGCCA TGG^CCA
1160 TGGCCA
BclI 353 TGATCA T^GATCA
1182 TGATCA
BglI 2275 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2833 GCCACCGAGGC
BsmFI 2069 GGGAC GGGAC(10/14)
1027 GTCCC
BsmI 1221 GAATGC GAATGC(1/-1)
983 GCATTC
BstYI 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
FbaI 353 TGATCA T^GATCA
1182 TGATCA
HgaI 2730 GACGC GACGC(5/10)
2560 GCGTC
KspI 224 CCGCGG CCGC^GG
2804 CCGCGG
MflI 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
MluNI 567 TGGCCA TGG^CCA
1160 TGGCCA
MscI 567 TGGCCA TGG^CCA
1160 TGGCCA
MslI 502 CACGTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2610 CATCAAAGTG
SacII 224 CCGCGG CCGC^GG
2804 CCGCGG
SmlI 607 CTTGAG C^T[CT][GA]AG
730 CTTGAG
StuI 2180 AGGCCT AGG^CCT
2418 AGGCCT
XhoII 1040 GGATCC [GA]^GATC[CT]
2201 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI