(Circular) (Six-base) MAPSORT of: pFN21AA0151.seq Check: 4668 from: 1 to: 6853 pFN21AA0151 6853 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2149 2388 2735 2810 3763 3873 2149 Size: 239 347 75 953 110 5129 Fragments arranged by size: 5129 953 347 239 110 75 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3201 4663 278 Size: 53 83 186 936 1665 1462 2468 Fragments arranged by size: 2468 1665 1462 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4196 1937 Size: 2259 4594 Acc65I G'GTAC_C Cuts at: 4174 4174 Size: 6853 AclI AA'CG_TT Cuts at: 1563 4980 5353 1563 Size: 3417 373 3063 Fragments arranged by size: 3417 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3133 3163 3491 3950 4916 6300 6568 1632 Size: 1501 30 328 459 966 1384 268 1917 Fragments arranged by size: 1917 1501 1384 966 459 328 268 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6656 AflIII A'CryG_T Cuts at: 1203 2553 5752 6729 1203 Size: 1350 3199 977 1327 Fragments arranged by size: 3199 1350 1327 977 AgeI A'CCGG_T Cuts at: 5684 5684 Size: 6853 AhdI GACnn_n'nnGTC Cuts at: 5582 5582 Size: 6853 AleI CACnn'nnGTG Cuts at: 1166 3078 1166 Size: 1912 4941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6853 AlwNI CAG_nnn'CTG Cuts at: 2651 3280 3469 4596 6168 2651 Size: 629 189 1127 1572 3336 Fragments arranged by size: 3336 1572 1127 629 189 ApaI G_GGCC'C Cuts at: 2772 3298 2772 Size: 526 6327 ApaLI G'TGCA_C Cuts at: 3020 3845 4910 6066 3020 Size: 825 1065 1156 3807 Fragments arranged by size: 3807 1156 1065 825 ApoI r'AATT_y Cuts at: 1493 3742 4162 4388 4540 6649 1493 Size: 2249 420 226 152 2109 1697 Fragments arranged by size: 2249 2109 1697 420 226 152 AseI AT'TA_AT Cuts at: 160 1032 5407 160 Size: 872 4375 1606 Fragments arranged by size: 4375 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 AvaI C'yCGr_G Cuts at: 1870 1943 2487 3086 4178 1870 Size: 73 544 599 1092 4545 Fragments arranged by size: 4545 1092 599 544 73 BamHI G'GATC_C Cuts at: 4183 4550 4183 Size: 367 6486 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 2836 3263 4174 5629 Size: 325 217 627 960 89 427 911 1455 Cuts at: 5629 618 Size: 1842 Fragments arranged by size: 1842 1455 960 911 627 427 325 217 89 BanII G_rGCy'C Cuts at: 1391 2306 2772 3093 3298 3988 4062 4172 1391 Size: 915 466 321 205 690 74 110 4072 Fragments arranged by size: 4072 915 690 466 321 205 110 74 BbeI G_GCGC'C Cuts at: 3267 3267 Size: 6853 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2113 2935 928 Size: 971 214 822 4846 Fragments arranged by size: 4846 971 822 214 BbvCI CC'TCA_GC Cuts at: 3407 3407 Size: 6853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5044 5078 1903 Size: 34 50 34 3023 34 3678 Fragments arranged by size: 3678 3023 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4745 5955 4745 Size: 1210 5643 BclI T'GATC_A Cuts at: 1548 1548 Size: 6853 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3876 5464 136 Size: 107 122 71 631 605 2204 1588 1525 Fragments arranged by size: 2204 1588 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6848 6848 Size: 6853 BlpI GC'TnA_GC Cuts at: 4270 4270 Size: 6853 Bme1580I G_kGCm'C Cuts at: 1790 2752 2772 2841 3024 3298 3849 4914 6070 Size: 962 20 69 183 274 551 1065 1156 Cuts at: 6070 1790 Size: 2573 Fragments arranged by size: 2573 1156 1065 962 551 274 183 69 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6853 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2825 3346 4584 Size: 416 53 83 399 24 1408 521 1238 Cuts at: 4584 5542 442 Size: 958 1753 Fragments arranged by size: 1753 1408 1238 958 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6853 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2287 3538 2287 Size: 1251 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2257 2318 2638 2795 2879 3338 3508 5513 Size: 1018 61 320 157 84 459 170 2005 Cuts at: 5513 1239 Size: 2579 Fragments arranged by size: 2579 2005 1018 459 320 170 157 84 61 Bpu10I CC'TnA_GC Cuts at: 3407 3563 3987 3407 Size: 156 424 6273 Fragments arranged by size: 6273 424 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4978 5843 6141 4978 Size: 865 298 5690 Fragments arranged by size: 5690 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2900 3542 3845 5516 882 Size: 617 1401 642 303 1671 2219 Fragments arranged by size: 2219 1671 1401 642 617 303 BsaAI yAC'GTr Cuts at: 493 1204 4644 493 Size: 711 3440 2702 Fragments arranged by size: 3440 2702 711 BsaBI GATnn'nnATC Cuts at: 6847 6847 Size: 6853 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3198 3264 4660 5042 Size: 53 83 186 936 1665 66 1396 382 Cuts at: 5042 5677 275 Size: 635 1451 Fragments arranged by size: 1665 1451 1396 936 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 2096 2709 5286 5684 5958 6105 6516 2096 Size: 613 2577 398 274 147 411 2433 Fragments arranged by size: 2577 2433 613 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2690 2720 3305 Size: 30 165 30 688 30 569 30 585 Cuts at: 3305 3335 1178 Size: 30 4696 Fragments arranged by size: 4696 688 585 569 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2459 2549 3389 3728 3800 1186 Size: 1054 219 90 840 339 72 4239 Fragments arranged by size: 4239 1054 840 339 219 90 72 BseYI C'CCAG_C Cuts at: 2047 2583 2732 2880 3092 4019 4135 6056 6552 Size: 536 149 148 212 927 116 1921 496 Cuts at: 6552 2047 Size: 2348 Fragments arranged by size: 2348 1921 927 536 496 212 149 148 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2177 2951 3317 3815 1163 Size: 573 441 774 366 498 4201 Fragments arranged by size: 4201 774 573 498 441 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4303 5064 6092 1540 Size: 275 186 2302 761 1028 2301 Fragments arranged by size: 2302 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2015 3024 3354 3849 4062 4914 4999 6070 2015 Size: 1009 330 495 213 852 85 1071 2798 Fragments arranged by size: 2798 1071 1009 852 495 330 213 85 BsmI GAATG_Cn' Cuts at: 2317 4364 4457 2317 Size: 2047 93 4713 Fragments arranged by size: 4713 2047 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2222 2521 3103 2222 Size: 299 582 5972 Fragments arranged by size: 5972 582 299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2306 2752 2772 2841 3024 3093 Size: 399 225 291 446 20 69 183 69 Cuts at: 3093 3298 3354 3849 3988 4062 4172 4914 4999 Size: 205 56 495 139 74 110 742 85 Cuts at: 4999 6070 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 495 446 399 291 225 205 183 139 110 85 74 69 69 56 20 BspEI T'CCGG_A Cuts at: 2096 6516 2096 Size: 4420 2433 BspHI T'CATG_A Cuts at: 4742 6472 4742 Size: 1730 5123 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2148 2149 2388 2550 2735 2810 3234 Size: 1208 96 1 239 162 185 75 424 Cuts at: 3234 3763 3873 4047 4208 844 Size: 529 110 174 161 3489 Fragments arranged by size: 3489 1208 529 424 239 185 174 162 161 110 96 75 1 BsrBI CCG'CTC Cuts at: 1394 1823 2563 4740 1394 Size: 429 740 2177 3507 Fragments arranged by size: 3507 2177 740 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2739 5348 5522 65 Size: 1183 1491 2609 174 1396 Fragments arranged by size: 2609 1491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2241 2366 2728 3266 5497 5684 1700 Size: 110 431 125 362 538 2231 187 2869 Fragments arranged by size: 2869 2231 538 431 362 187 125 110 BsrGI T'GTAC_A Cuts at: 96 2073 96 Size: 1977 4876 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6853 BssSI C'ACGA_G Cuts at: 3848 4913 5925 3848 Size: 1065 1012 4776 Fragments arranged by size: 4776 1065 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6853 BstBI TT'CG_AA Cuts at: 2575 5657 2575 Size: 3082 3771 BstEII G'GTnAC_C Cuts at: 1184 3071 1184 Size: 1887 4966 BstXI CCAn_nnnn'nTGG Cuts at: 2857 2857 Size: 6853 BstYI r'GATC_y Cuts at: 1972 2611 3459 4183 4550 4935 4952 6404 6490 Size: 639 848 724 367 385 17 1452 86 Cuts at: 6490 6502 6848 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 848 724 639 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2328 3165 2328 Size: 837 6016 BtgI C'CryG_G Cuts at: 513 1065 2684 2718 513 Size: 552 1619 34 4648 Fragments arranged by size: 4648 1619 552 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2387 3097 487 Size: 683 56 1161 710 4243 Fragments arranged by size: 4243 1161 710 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3243 4369 5163 5183 2061 Size: 1182 1126 794 20 3731 Fragments arranged by size: 3731 1182 1126 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6853 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6818 DraI TTT'AAA Cuts at: 4157 4157 Size: 6853 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5860 809 Size: 507 4544 1802 Fragments arranged by size: 4544 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2847 4300 Size: 54 1425 50 275 18 534 483 1453 Cuts at: 4300 5189 6818 8 Size: 889 1629 43 Fragments arranged by size: 1629 1453 1425 889 534 483 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4300 1537 Size: 275 2488 4090 Fragments arranged by size: 4090 2488 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3152 3584 3939 4783 1353 Size: 1799 432 355 844 3423 Fragments arranged by size: 3423 1799 844 432 355 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2250 2704 3248 5440 5814 5960 Size: 1064 138 661 454 544 2192 374 146 Cuts at: 5960 387 Size: 1280 Fragments arranged by size: 2192 1280 1064 661 544 454 374 146 138 EcoICRI GAG'CTC Cuts at: 4060 4060 Size: 6853 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2257 2318 2638 2795 2879 3133 3163 Size: 393 625 61 320 157 84 254 30 Cuts at: 3163 3338 3491 3508 3950 4916 5513 6300 6568 Size: 175 153 17 442 966 597 787 268 Cuts at: 6568 1239 Size: 1524 Fragments arranged by size: 1524 966 787 625 597 442 393 320 268 254 175 157 153 84 61 30 17 EcoNI CCTnn'n_nnAGG Cuts at: 2954 3065 2954 Size: 111 6742 EcoO109I rG'GnC_Cy Cuts at: 2176 2769 3184 3277 3295 3517 4036 2176 Size: 593 415 93 18 222 519 4993 Fragments arranged by size: 4993 593 519 415 222 93 18 EcoRI G'AATT_C Cuts at: 4162 4540 4162 Size: 378 6475 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3673 3673 Size: 6853 FspI TGC'GCA Cuts at: 5359 5359 Size: 6853 HaeII r_GCGC'y Cuts at: 1678 2484 3267 6000 1678 Size: 806 783 2733 2531 Fragments arranged by size: 2733 2531 806 783 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2596 2628 2919 2951 3583 Size: 32 135 32 1024 32 291 32 632 Cuts at: 3583 3615 5494 5526 5568 5600 1373 Size: 32 1879 32 42 32 2626 Fragments arranged by size: 2626 1879 1024 632 291 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4197 4443 669 Size: 988 281 2259 246 3079 Fragments arranged by size: 3079 2259 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6853 HpaI GTT'AAC Cuts at: 4443 4443 Size: 6853 KasI G'GCGC_C Cuts at: 3263 3263 Size: 6853 KpnI G_GTAC'C Cuts at: 4178 4178 Size: 6853 MfeI C'AATT_G Cuts at: 4452 4452 Size: 6853 MluI A'CGCG_T Cuts at: 6729 6729 Size: 6853 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5967 6151 1305 Size: 4662 184 2007 Fragments arranged by size: 4662 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2849 10 Size: 54 1425 343 1017 4014 Fragments arranged by size: 4014 1425 1017 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2037 2627 3078 3347 4811 5170 5329 Size: 648 871 590 451 269 1464 359 159 Cuts at: 5329 518 Size: 2042 Fragments arranged by size: 2042 1464 871 648 590 451 359 269 159 MspA1I CmG'CkG Cuts at: 1606 2809 2833 4269 4946 6094 6339 1606 Size: 1203 24 1436 677 1148 245 2120 Fragments arranged by size: 2120 1436 1203 1148 677 245 24 NaeI GCC'GGC Cuts at: 2243 2368 2730 3268 2243 Size: 125 362 538 5828 Fragments arranged by size: 5828 538 362 125 NarI GG'CG_CC Cuts at: 3264 3264 Size: 6853 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6301 NdeI CA'TA_TG Cuts at: 387 387 Size: 6853 NgoMIV G'CCGG_C Cuts at: 2241 2366 2728 3266 2241 Size: 125 362 538 5828 Fragments arranged by size: 5828 538 362 125 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6853 NotI GC'GGCC_GC Cuts at: 4300 4300 Size: 6853 NruI TCG'CGA Cuts at: 2406 2406 Size: 6853 NspI r_CATG'y Cuts at: 1132 1229 2557 3366 4054 4211 5756 1132 Size: 97 1328 809 688 157 1545 2229 Fragments arranged by size: 2229 1545 1328 809 688 157 97 PasI CC'CwG_GG Cuts at: 1348 3910 1348 Size: 2562 4291 PciI A'CATG_T Cuts at: 2553 5752 2553 Size: 3199 3654 PflMI CCAn_nnn'nTGG Cuts at: 1108 2880 3701 1108 Size: 1772 821 4260 Fragments arranged by size: 4260 1772 821 PfoI T'CCnGG_A Cuts at: 2856 3009 3462 3780 3918 3939 4095 2856 Size: 153 453 318 138 21 156 5614 Fragments arranged by size: 5614 453 318 156 153 138 21 PmeI GTTT'AAAC Cuts at: 4157 4157 Size: 6853 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2353 4922 4954 6435 6467 2321 Size: 32 2569 32 1481 32 2707 Fragments arranged by size: 2707 2569 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 3184 3517 4036 2176 Size: 1008 333 519 4993 Fragments arranged by size: 4993 1008 519 333 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6853 PsiI TTA'TAA Cuts at: 4423 4423 Size: 6853 PspOMI G'GGCC_C Cuts at: 2768 3294 2768 Size: 526 6327 PspXI vC'TCGA_Gb Cuts at: 3086 3086 Size: 6853 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3647 3679 3647 Size: 32 6821 PstI C_TGCA'G Cuts at: 830 2826 2925 3231 3484 3774 4205 830 Size: 1996 99 306 253 290 431 3478 Fragments arranged by size: 3478 1996 431 306 290 253 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6853 PvuII CAG'CTG Cuts at: 2809 2833 2809 Size: 24 6829 SacI G_AGCT'C Cuts at: 4062 4062 Size: 6853 SalI G'TCGA_C Cuts at: 1936 4195 1936 Size: 2259 4594 SanDI GG'GwC_CC Cuts at: 3184 3184 Size: 6853 SapI GCTCTTCn'nnn_ Cuts at: 3584 3939 3584 Size: 355 6498 SbfI CC_TGCA'GG Cuts at: 3231 3774 4205 3231 Size: 543 431 5879 Fragments arranged by size: 5879 543 431 ScaI AGT'ACT Cuts at: 2267 5101 6813 2267 Size: 2834 1712 2307 Fragments arranged by size: 2834 2307 1712 SfcI C'TryA_G Cuts at: 826 1045 2822 2921 3227 3480 3770 4201 5336 Size: 219 1777 99 306 253 290 431 1135 Cuts at: 5336 6017 6208 826 Size: 681 191 1471 Fragments arranged by size: 1777 1471 1135 681 431 306 290 253 219 191 99 SfoI GGC'GCC Cuts at: 3265 3265 Size: 6853 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6853 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6853 SmaI CCC'GGG Cuts at: 1872 2489 4180 1872 Size: 617 1691 4545 Fragments arranged by size: 4545 1691 617 SmlI C'TyrA_G Cuts at: 820 1017 1943 3086 4957 5858 6120 820 Size: 197 926 1143 1871 901 262 1553 Fragments arranged by size: 1871 1553 1143 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4644 493 Size: 4151 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6853 SphI G_CATG'C Cuts at: 1132 4211 1132 Size: 3079 3774 SspI AAT'ATT Cuts at: 5 52 4777 5 Size: 47 4725 2081 Fragments arranged by size: 4725 2081 47 StuI AGG'CCT Cuts at: 1517 3992 1517 Size: 2475 4378 StyI C'CwwG_G Cuts at: 513 1065 3249 4292 513 Size: 552 2184 1043 3074 Fragments arranged by size: 3074 2184 1043 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 3987 4880 4897 5050 5235 5680 Size: 1255 929 1478 893 17 153 185 445 Cuts at: 5680 325 Size: 1498 Fragments arranged by size: 1498 1478 1255 929 893 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 2265 5099 Size: 274 80 33 51 1443 96 192 2834 Cuts at: 5099 6811 96 Size: 1712 138 Fragments arranged by size: 2834 1712 1443 274 192 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6813 6845 6813 Size: 32 6821 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6853 XbaI T'CTAG_A Cuts at: 4189 4189 Size: 6853 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2648 3256 2648 Size: 608 6245 XhoI C'TCGA_G Cuts at: 1943 3086 1943 Size: 1143 5710 XmaI C'CCGG_G Cuts at: 1870 2487 4178 1870 Size: 617 1691 4545 Fragments arranged by size: 4545 1691 617 XmnI GAAnn'nnTTC Cuts at: 2120 4982 2120 Size: 2862 3991 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3199 4661 276 Size: 53 83 186 936 1665 1462 2468 Fragments arranged by size: 2468 1665 1462 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NsiI PacI PmlI RsrII SacII SexAI SfiI SrfI SwaI