Restriction map for
pFN21AB9887
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BciVI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MflI
MluI MslI MunI NcoI NdeI
NheI NotI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 1123 GAATTC [GA]^AATT[CT]
AflII 771 CTTAAG C^TTAAG
AhdI 1069 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1423 GATCC GGATC(4/5)
AocI 268 CCTGAGG CC^T[ACGT]AGG
ApaI 546 GGGCCC GGGCC^C
ApoI 1123 GAATTC [GA]^AATT[CT]
Asp700I 1666 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1069 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2163 GGCGCC GGCGC^C
BbsI 1372 GTCTTC GAAGAC(2/6)
BcgI 1352 CGAAAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 743 TGATCA T^GATCA
BfrI 771 CTTAAG C^TTAAG
BpuAI 1372 GTCTTC GAAGAC(2/6)
BsmI 1524 GAATGC GAATGC(1/-1)
BsrDI 2150 GCAATG GCAATG(2/0)
BstAPI 1818 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2195 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
DraIII 25 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1069 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
EcoRI 1123 GAATTC G^AATTC
FbaI 743 TGATCA T^GATCA
KasI 2163 GGCGCC G^GCGCC
MseI 772 TTAA T^TAA
NaeI 1330 GCCGGC GCC^GGC
NarI 2163 GGCGCC GG^CGCC
NgoMIV 1330 GCCGGC G^CCGGC
NruI 444 TCGCGA TCG^CGA
SapI 304 GCTCTTC GCTCTTC(1/4)
SfoI 2163 GGCGCC GGC^GCC
SmaI 565 CCCGGG CCC^GGG
SspI 86 AATATT AAT^ATT
Tru9I 772 TTAA T^TAA
XbaI 957 TCTAGA T^CTAGA
XmaCI 565 CCCGGG C^CCGGG
XmaI 565 CCCGGG C^CCGGG
XmnI 1666 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1542 GTAC GT^AC
1821 GTAC
AlwNI 1819 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1849 CAGCGGCTG
Aor51HI 277 AGCGCT AGC^GCT
290 AGCGCT
BbrPI 181 CACGTG CAC^GTG
1886 CACGTG
BfaI 79 CTAG C^TAG
958 CTAG
BsaAI 181 CACGTG [CT]AC^GT[GA]
1886 CACGTG
BsaI 420 GAGACC GGTCTC(1/5)
434 GGTCTC
BsmBI 686 CGTCTC CGTCTC(1/5)
1988 GAGACG
BspMI 1823 ACCTGC ACCTGC(4/8)
42 GCAGGT
BsrFI 1330 GCCGGC [GA]^CCGG[CT]
1536 GCCGGT
BssSI 232 CTCGTG CACGAG(-5/-1)
1259 CTCGTG
Cfr10I 1330 GCCGGC [GA]^CCGG[CT]
1536 GCCGGT
DsaI 2033 CCGCGG C^C[GA][CT]GG
2168 CCGCGG
Eco47III 277 AGCGCT AGC^GCT
290 AGCGCT
EcoNI 1089 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1392 CCTCTCAGAGG
KspI 2033 CCGCGG CCGC^GG
2168 CCGCGG
MaeI 79 CTAG C^TAG
958 CTAG
MaeIII 908 GTCAC ^GT[ACGT]AC
932 GTCAC
PflMI 177 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
973 CCAGGAAATGG
PmaCI 181 CACGTG CAC^GTG
1886 CACGTG
PmlI 181 CACGTG CAC^GTG
1886 CACGTG
PvuII 485 CAGCTG CAG^CTG
1696 CAGCTG
RsaI 1542 GTAC GT^AC
1821 GTAC
SacI 1083 GAGCTC GAGCT^C
1381 GAGCTC
SacII 2033 CCGCGG CCGC^GG
2168 CCGCGG
SmlI 771 CTTAAG C^T[CT][GA]AG
1037 CTCAAG
Tsp45I 908 GTCAC ^GT[GC]AC
932 GTCAC
Van91I 177 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
973 CCAGGAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AccIII AciI AcyI AluI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MboI MboII MluNI MnlI
MroI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI TseI Tsp509I
TspRI TthHB8I