(Circular) (Six-base) MAPSORT of: pFN21AB9887.seq Check: 6216 from: 1 to: 6964 pFN21AB9887 6964 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2038 2038 Size: 6964 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4774 278 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4307 1937 Size: 2370 4594 Acc65I G'GTAC_C Cuts at: 4285 4285 Size: 6964 AclI AA'CG_TT Cuts at: 1563 5091 5464 1563 Size: 3528 373 3063 Fragments arranged by size: 3528 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 5027 6411 6679 1632 Size: 665 2730 1384 268 1917 Fragments arranged by size: 2730 1917 1384 665 268 AfeI AGC'GCT Cuts at: 2284 2297 2284 Size: 13 6951 AflII C'TTAA_G Cuts at: 820 1017 2776 820 Size: 197 1759 5008 Fragments arranged by size: 5008 1759 197 AflIII A'CryG_T Cuts at: 1203 5863 6840 1203 Size: 4660 977 1327 Fragments arranged by size: 4660 1327 977 AgeI A'CCGG_T Cuts at: 5795 5795 Size: 6964 AhdI GACnn_n'nnGTC Cuts at: 3079 5693 3079 Size: 2614 4350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6964 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6964 AlwNI CAG_nnn'CTG Cuts at: 3829 3859 4707 6279 3829 Size: 30 848 1572 4514 Fragments arranged by size: 4514 1572 848 30 ApaI G_GGCC'C Cuts at: 2555 2555 Size: 6964 ApaLI G'TGCA_C Cuts at: 5021 6177 5021 Size: 1156 5808 ApoI r'AATT_y Cuts at: 1493 3128 4273 4499 4651 6760 1493 Size: 1635 1145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1635 1145 226 152 AseI AT'TA_AT Cuts at: 160 1032 5518 160 Size: 872 4486 1606 Fragments arranged by size: 4486 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 AvaI C'yCGr_G Cuts at: 1870 1943 2434 2546 2570 3005 3550 4289 1870 Size: 73 491 112 24 435 545 739 4545 Fragments arranged by size: 4545 739 545 491 435 112 73 24 BamHI G'GATC_C Cuts at: 4294 4661 4294 Size: 367 6597 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3364 3580 4168 4285 5740 Size: 325 217 627 1577 216 588 117 1455 Cuts at: 5740 618 Size: 1842 Fragments arranged by size: 1842 1577 1455 627 588 325 217 216 117 BanII G_rGCy'C Cuts at: 1391 2369 2457 2555 2562 3092 3390 3557 4283 Size: 978 88 98 7 530 298 167 726 Cuts at: 4283 1391 Size: 4072 Fragments arranged by size: 4072 978 726 530 298 167 98 88 7 BbeI G_GCGC'C Cuts at: 4172 4172 Size: 6964 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3370 928 Size: 971 1471 4522 Fragments arranged by size: 4522 1471 971 BbvCI CC'TCA_GC Cuts at: 2928 3123 3635 2928 Size: 195 512 6257 Fragments arranged by size: 6257 512 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3346 3380 5155 5189 1903 Size: 34 1409 34 1775 34 3678 Fragments arranged by size: 3678 1775 1409 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4856 6066 4856 Size: 1210 5754 BclI T'GATC_A Cuts at: 1548 2748 1548 Size: 1200 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5575 136 Size: 107 122 71 631 605 337 3566 1525 Fragments arranged by size: 3566 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6959 6959 Size: 6964 BlpI GC'TnA_GC Cuts at: 4381 4381 Size: 6964 Bme1580I G_kGCm'C Cuts at: 1790 2477 2555 2685 2722 3263 3369 3607 5025 Size: 687 78 130 37 541 106 238 1418 Cuts at: 5025 6181 1790 Size: 1156 2573 Fragments arranged by size: 2573 1418 1156 687 541 238 130 106 78 37 BmgBI CAC'GTC Cuts at: 1318 3643 1318 Size: 2325 4639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3670 4695 Size: 416 53 83 399 24 1535 718 1025 Cuts at: 4695 5653 442 Size: 958 1753 Fragments arranged by size: 1753 1535 1025 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2388 2388 Size: 6964 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2889 3541 3560 4045 5624 1239 Size: 1650 652 19 485 1579 2579 Fragments arranged by size: 2579 1650 1579 652 485 19 Bpu10I CC'TnA_GC Cuts at: 2382 2556 2607 2928 3123 3635 4145 2382 Size: 174 51 321 195 512 510 5201 Fragments arranged by size: 5201 512 510 321 195 174 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3027 5089 5954 6252 3027 Size: 2062 865 298 3739 Fragments arranged by size: 3739 2062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 2445 5627 882 Size: 617 920 26 3182 2219 Fragments arranged by size: 3182 2219 920 617 26 BsaAI yAC'GTr Cuts at: 493 1204 2188 3893 4755 493 Size: 711 984 1705 862 2702 Fragments arranged by size: 2702 1705 984 862 711 BsaBI GATnn'nnATC Cuts at: 6958 6958 Size: 6964 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3630 3843 3953 4169 Size: 53 83 186 936 2097 213 110 216 Cuts at: 4169 4771 5153 5788 275 Size: 602 382 635 1451 Fragments arranged by size: 2097 1451 936 635 602 382 216 213 186 110 83 53 BsaWI w'CCGG_w Cuts at: 2538 3625 3838 5397 5795 6069 6216 6627 2538 Size: 1087 213 1559 398 274 147 411 2875 Fragments arranged by size: 2875 1559 1087 411 398 274 213 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 4043 4073 1178 Size: 30 165 30 2136 30 474 30 4069 Fragments arranged by size: 4069 2136 474 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2912 3023 3122 1186 Size: 1105 621 111 99 5028 Fragments arranged by size: 5028 1105 621 111 99 BseYI C'CCAG_C Cuts at: 2488 3368 6167 6663 2488 Size: 880 2799 496 2789 Fragments arranged by size: 2799 2789 880 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2012 3204 3873 1163 Size: 573 276 1192 669 4254 Fragments arranged by size: 4254 1192 669 573 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2503 2971 3034 3336 4414 5175 Size: 275 186 502 468 63 302 1078 761 Cuts at: 5175 6203 1540 Size: 1028 2301 Fragments arranged by size: 2301 1078 1028 761 502 468 302 275 186 63 BsiHKAI G_wGCw'C Cuts at: 2238 2388 3092 3390 4164 5025 5110 6181 2238 Size: 150 704 298 774 861 85 1071 3021 Fragments arranged by size: 3021 1071 861 774 704 298 150 85 BsmI GAATG_Cn' Cuts at: 3535 4475 4568 3535 Size: 940 93 5931 Fragments arranged by size: 5931 940 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2697 3987 2697 Size: 1290 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2369 2388 2457 2477 2555 2562 Size: 399 448 131 19 69 20 78 7 Cuts at: 2562 2685 2722 3092 3263 3369 3390 3557 3607 Size: 123 37 370 171 106 21 167 50 Cuts at: 3607 4164 4283 5025 5110 6181 1391 Size: 557 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 448 399 370 171 167 131 123 119 106 85 78 69 50 37 21 20 19 7 BspEI T'CCGG_A Cuts at: 2538 3625 3838 6627 2538 Size: 1087 213 2789 2875 Fragments arranged by size: 2875 2789 1087 213 BspHI T'CATG_A Cuts at: 4853 6583 4853 Size: 1730 5234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 3837 4319 844 Size: 1194 1799 482 3489 Fragments arranged by size: 3489 1799 1194 482 BsrBI CCG'CTC Cuts at: 1394 1823 4851 1394 Size: 429 3028 3507 Fragments arranged by size: 3507 3028 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4162 5459 5633 65 Size: 1183 2914 1297 174 1396 Fragments arranged by size: 2914 1396 1297 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3335 3541 5608 5795 1700 Size: 110 1525 206 2067 187 2869 Fragments arranged by size: 2869 2067 1525 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6964 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6964 BssSI C'ACGA_G Cuts at: 2237 3264 5024 6036 2237 Size: 1027 1760 1012 3165 Fragments arranged by size: 3165 1760 1027 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3829 794 Size: 3035 3929 BstBI TT'CG_AA Cuts at: 5768 5768 Size: 6964 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6964 BstXI CCAn_nnnn'nTGG Cuts at: 4207 4207 Size: 6964 BstYI r'GATC_y Cuts at: 1972 4294 4661 5046 5063 6515 6601 6613 6959 Size: 2322 367 385 17 1452 86 12 346 Cuts at: 6959 1972 Size: 1977 Fragments arranged by size: 2322 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2274 Size: 6964 BtgI C'CryG_G Cuts at: 513 1065 2004 4038 4173 513 Size: 552 939 2034 135 3304 Fragments arranged by size: 3304 2034 939 552 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6225 Fragments arranged by size: 6225 683 56 BtsI GCAGTG_nn' Cuts at: 2040 3216 3605 4480 5274 5294 2040 Size: 1176 389 875 794 20 3710 Fragments arranged by size: 3710 1176 875 794 389 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3205 3240 529 Size: 35 2641 35 4253 Fragments arranged by size: 4253 2641 35 35 DraI TTT'AAA Cuts at: 4268 4268 Size: 6964 DraIII CAC_nnn'GTG Cuts at: 2035 2035 Size: 6964 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5971 809 Size: 507 4655 1802 Fragments arranged by size: 4655 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2500 2875 Size: 54 1425 50 275 18 228 442 375 Cuts at: 2875 2968 3333 3945 4176 4221 4411 5300 6929 Size: 93 365 612 231 45 190 889 1629 Cuts at: 6929 8 Size: 43 Fragments arranged by size: 1629 1425 889 612 442 375 365 275 231 228 190 93 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2500 2968 3333 4411 1537 Size: 275 688 468 365 1078 4090 Fragments arranged by size: 4090 1078 688 468 365 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2581 2915 2945 3487 4894 1353 Size: 963 265 334 30 542 1407 3423 Fragments arranged by size: 3423 1407 963 542 334 265 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2098 2153 3337 5551 5925 6071 Size: 1064 138 509 55 1184 2214 374 146 Cuts at: 6071 387 Size: 1280 Fragments arranged by size: 2214 1280 1184 1064 509 374 146 138 55 EcoICRI GAG'CTC Cuts at: 3090 3388 3090 Size: 298 6666 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2889 3541 3560 4045 5027 5624 Size: 393 665 592 652 19 485 982 597 Cuts at: 5624 6411 6679 1239 Size: 787 268 1524 Fragments arranged by size: 1524 982 787 665 652 597 592 485 393 268 19 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3401 3098 Size: 303 6661 EcoO109I rG'GnC_Cy Cuts at: 2269 2551 2552 2769 2974 3394 3652 2269 Size: 282 1 217 205 420 258 5581 Fragments arranged by size: 5581 420 282 258 217 205 1 EcoRI G'AATT_C Cuts at: 3128 4273 4651 3128 Size: 1145 378 5441 Fragments arranged by size: 5441 1145 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3038 3038 Size: 6964 FspI TGC'GCA Cuts at: 5470 5470 Size: 6964 HaeII r_GCGC'y Cuts at: 1678 2170 2286 2299 3342 4172 6111 1678 Size: 492 116 13 1043 830 1939 2531 Fragments arranged by size: 2531 1939 1043 830 492 116 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2720 2752 2944 2976 3065 Size: 32 135 32 1148 32 192 32 89 Cuts at: 3065 3097 3431 3463 3682 3714 5605 5637 5679 Size: 32 334 32 219 32 1891 32 42 Cuts at: 5679 5711 1373 Size: 32 2626 Fragments arranged by size: 2626 1891 1148 334 219 192 135 89 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4308 4554 669 Size: 988 281 2370 246 3079 Fragments arranged by size: 3079 2370 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6964 HpaI GTT'AAC Cuts at: 4554 4554 Size: 6964 KasI G'GCGC_C Cuts at: 4168 4168 Size: 6964 KpnI G_GTAC'C Cuts at: 4289 4289 Size: 6964 MfeI C'AATT_G Cuts at: 4563 4563 Size: 6964 MluI A'CGCG_T Cuts at: 6840 6840 Size: 6964 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2543 3051 6078 6262 1305 Size: 1238 508 3027 184 2007 Fragments arranged by size: 3027 2007 1238 508 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 3947 4223 10 Size: 54 1425 343 228 1887 276 2751 Fragments arranged by size: 2751 1887 1425 343 276 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 4922 5281 5440 518 Size: 648 3756 359 159 2042 Fragments arranged by size: 3756 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2492 2564 2874 3703 3856 4040 4077 4175 Size: 886 72 310 829 153 184 37 98 Cuts at: 4175 4216 4380 5057 6205 6450 1606 Size: 41 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 886 829 677 310 245 184 164 153 98 72 41 37 NaeI GCC'GGC Cuts at: 3337 3337 Size: 6964 NarI GG'CG_CC Cuts at: 4169 4169 Size: 6964 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5473 Fragments arranged by size: 5473 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6964 NgoMIV G'CCGG_C Cuts at: 3335 3335 Size: 6964 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6964 NotI GC'GGCC_GC Cuts at: 4411 4411 Size: 6964 NruI TCG'CGA Cuts at: 2451 2451 Size: 6964 NspI r_CATG'y Cuts at: 1132 1229 4322 5867 1132 Size: 97 3093 1545 2229 Fragments arranged by size: 3093 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2650 2722 1348 Size: 1302 72 5590 Fragments arranged by size: 5590 1302 72 PciI A'CATG_T Cuts at: 5863 5863 Size: 6964 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 2984 1108 Size: 1080 796 5088 Fragments arranged by size: 5088 1080 796 PfoI T'CCnGG_A Cuts at: 3303 3495 3774 3303 Size: 192 279 6493 Fragments arranged by size: 6493 279 192 PmeI GTTT'AAAC Cuts at: 4268 4268 Size: 6964 PmlI CAC'GTG Cuts at: 2188 3893 2188 Size: 1705 5259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5033 5065 6546 6578 5033 Size: 32 1481 32 5419 Fragments arranged by size: 5419 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2974 3394 3652 2269 Size: 705 420 258 5581 Fragments arranged by size: 5581 705 420 258 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6964 PsiI TTA'TAA Cuts at: 4534 4534 Size: 6964 PspOMI G'GGCC_C Cuts at: 2551 2551 Size: 6964 PstI C_TGCA'G Cuts at: 830 2049 3330 3991 4316 830 Size: 1219 1281 661 325 3478 Fragments arranged by size: 3478 1281 1219 661 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6964 PvuII CAG'CTG Cuts at: 2492 3703 2492 Size: 1211 5753 SacI G_AGCT'C Cuts at: 3092 3390 3092 Size: 298 6666 SacII CC_GC'GG Cuts at: 4041 4176 4041 Size: 135 6829 SalI G'TCGA_C Cuts at: 1936 4306 1936 Size: 2370 4594 SanDI GG'GwC_CC Cuts at: 2974 2974 Size: 6964 SapI GCTCTTCn'nnn_ Cuts at: 2316 2316 Size: 6964 SbfI CC_TGCA'GG Cuts at: 4316 4316 Size: 6964 ScaI AGT'ACT Cuts at: 5212 6924 5212 Size: 1712 5252 SfcI C'TryA_G Cuts at: 826 1045 2045 3326 3987 4312 5447 6128 6319 Size: 219 1000 1281 661 325 1135 681 191 Cuts at: 6319 826 Size: 1471 Fragments arranged by size: 1471 1281 1135 1000 681 661 325 219 191 SfoI GGC'GCC Cuts at: 4170 4170 Size: 6964 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6964 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6964 SmaI CCC'GGG Cuts at: 1872 2572 4291 1872 Size: 700 1719 4545 Fragments arranged by size: 4545 1719 700 SmlI C'TyrA_G Cuts at: 820 1017 1943 2776 3042 5068 5969 6231 820 Size: 197 926 833 266 2026 901 262 1553 Fragments arranged by size: 2026 1553 926 901 833 266 262 197 SnaBI TAC'GTA Cuts at: 493 4755 493 Size: 4262 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6964 SphI G_CATG'C Cuts at: 1132 4322 1132 Size: 3190 3774 SspI AAT'ATT Cuts at: 5 52 2093 4888 5 Size: 47 2041 2795 2081 Fragments arranged by size: 2795 2081 2041 47 StuI AGG'CCT Cuts at: 1517 2330 2926 3407 3562 1517 Size: 813 596 481 155 4919 Fragments arranged by size: 4919 813 596 481 155 StyI C'CwwG_G Cuts at: 513 1065 2004 2792 2892 3255 4224 4403 513 Size: 552 939 788 100 363 969 179 3074 Fragments arranged by size: 3074 969 939 788 552 363 179 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2259 4991 5008 5161 5346 5791 325 Size: 1255 679 2732 17 153 185 445 1498 Fragments arranged by size: 2732 1498 1255 679 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3546 5210 6922 Size: 274 80 33 51 1443 1569 1664 1712 Cuts at: 6922 96 Size: 138 Fragments arranged by size: 1712 1664 1569 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3477 3509 3510 3542 4043 4075 6924 6956 3477 Size: 32 1 32 501 32 2849 32 3485 Fragments arranged by size: 3485 2849 501 32 32 32 32 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6964 XbaI T'CTAG_A Cuts at: 2962 4300 2962 Size: 1338 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6964 XmaI C'CCGG_G Cuts at: 1870 2570 4289 1870 Size: 700 1719 4545 Fragments arranged by size: 4545 1719 700 XmnI GAAnn'nnTTC Cuts at: 3675 5093 3675 Size: 1418 5546 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4772 276 Size: 53 83 186 936 3238 2468 Fragments arranged by size: 3238 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NsiI PacI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI