Restriction map for
pFN21AA0131
(Circular) (Six-base) MAPSORT of: pFN21AA0131.seq Check: 1101 from: 1 to: 7543
pFN21AA0131 7543 bp
With 182 enzymes: SgfI *
January 16, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2839 4420 2839
Size: 1581 5962
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2799 5353 278
Size: 53 83 186 936 1263 2554 2468
Fragments arranged by size:
2554 2468 1263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4119 4886 1937
Size: 2182 767 4594
Fragments arranged by size:
4594 2182 767
Acc65I G'GTAC_C
Cuts at: 4864 4864
Size: 7543
AclI AA'CG_TT
Cuts at: 1563 5670 6043 1563
Size: 4107 373 3063
Fragments arranged by size:
4107 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3173 3392 3767 5606 6990 7258 1632
Size: 1541 219 375 1839 1384 268 1917
Fragments arranged by size:
1917 1839 1541 1384 375 268 219
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 7543
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7346
AflIII A'CryG_T
Cuts at: 1203 4594 4840 6442 7419 1203
Size: 3391 246 1602 977 1327
Fragments arranged by size:
3391 1602 1327 977 246
AgeI A'CCGG_T
Cuts at: 4000 6374 4000
Size: 2374 5169
AhdI GACnn_n'nnGTC
Cuts at: 6272 6272
Size: 7543
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7543
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7543
AlwNI CAG_nnn'CTG
Cuts at: 2149 2398 2461 2908 3172 4297 5286 6858 2149
Size: 249 63 447 264 1125 989 1572 2834
Fragments arranged by size:
2834 1572 1125 989 447 264 249 63
ApaI G_GGCC'C
Cuts at: 2385 2640 3990 4716 4778 4786 2385
Size: 255 1350 726 62 8 5142
Fragments arranged by size:
5142 1350 726 255 62 8
ApaLI G'TGCA_C
Cuts at: 2719 5600 6756 2719
Size: 2881 1156 3506
Fragments arranged by size:
3506 2881 1156
ApoI r'AATT_y
Cuts at: 1493 3464 4852 5078 5230 7339 1493
Size: 1971 1388 226 152 2109 1697
Fragments arranged by size:
2109 1971 1697 1388 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6097 160
Size: 872 5065 1606
Fragments arranged by size:
5065 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7543
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2969 4342 4478 4770 4868 1870
Size: 73 253 773 1373 136 292 98 4545
Fragments arranged by size:
4545 1373 773 292 253 136 98 73
BamHI G'GATC_C
Cuts at: 2941 4873 5240 2941
Size: 1932 367 5244
Fragments arranged by size:
5244 1932 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2424 2621 3641 4003
Size: 325 217 627 379 258 197 1020 362
Cuts at: 4003 4135 4864 6319 618
Size: 132 729 1455 1842
Fragments arranged by size:
1842 1455 1020 729 627 379 362 325
258 217 197 132
BanII G_rGCy'C
Cuts at: 1391 2385 2475 2496 2640 2654 3091 3551 3774
Size: 994 90 21 144 14 437 460 223
Cuts at: 3774 3990 4650 4716 4778 4786 4862 1391
Size: 216 660 66 62 8 76 4072
Fragments arranged by size:
4072 994 660 460 437 223 216 144
90 76 66 62 21 14 8
BbeI G_GCGC'C
Cuts at: 2170 2428 2170
Size: 258 7285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 2980 4078 928
Size: 971 617 464 1098 4393
Fragments arranged by size:
4393 1098 971 617 464
BbvCI CC'TCA_GC
Cuts at: 2175 4798 2175
Size: 2623 4920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2960 2994 4061 4095 5734 5768 1903
Size: 34 1023 34 1067 34 1639 34 3678
Fragments arranged by size:
3678 1639 1067 1023 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5435 6645 5435
Size: 1210 6333
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7543
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2238 2630 4012
Size: 107 122 71 631 605 566 392 1382
Cuts at: 4012 4749 6154 136
Size: 737 1405 1525
Fragments arranged by size:
1525 1405 1382 737 631 605 566 392
122 107 71
BglII A'GATC_T
Cuts at: 3045 7538 3045
Size: 4493 3050
BlpI GC'TnA_GC
Cuts at: 2087 2747 4298 4960 2087
Size: 660 1551 662 4670
Fragments arranged by size:
4670 1551 662 660
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2640 2723 3567 3646 3990 4008 4716
Size: 595 255 83 844 79 344 18 708
Cuts at: 4716 4778 4786 5604 6760 1790
Size: 62 8 818 1156 2573
Fragments arranged by size:
2573 1156 844 818 708 595 344 255
83 79 62 18 8
BmgBI CAC'GTC
Cuts at: 1318 2419 1318
Size: 1101 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3442 3493 3928
Size: 416 53 83 399 24 2025 51 435
Cuts at: 3928 4698 5274 6232 442
Size: 770 576 958 1753
Fragments arranged by size:
2025 1753 958 770 576 435 416 399
83 53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7543
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2503 4357 2242
Size: 261 1854 5428
Fragments arranged by size:
5428 1854 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2212 2471 2480 2498 2931 3197 3320
Size: 890 83 259 9 18 433 266 123
Cuts at: 3320 3788 3845 4193 4259 6203 1239
Size: 468 57 348 66 1944 2579
Fragments arranged by size:
2579 1944 890 468 433 348 266 259
123 83 66 57 18 9
Bpu10I CC'TnA_GC
Cuts at: 2175 3550 4798 2175
Size: 1375 1248 4920
Fragments arranged by size:
4920 1375 1248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2642 2699 3230 5668 6533 6831 2642
Size: 57 531 2438 865 298 3354
Fragments arranged by size:
3354 2438 865 531 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 3302 3661 6206 882
Size: 617 992 811 359 2545 2219
Fragments arranged by size:
2545 2219 992 811 617 359
BsaAI yAC'GTr
Cuts at: 493 1204 3853 4597 5334 493
Size: 711 2649 744 737 2702
Fragments arranged by size:
2702 2649 744 737 711
BsaBI GATnn'nnATC
Cuts at: 7537 7537
Size: 7543
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2167 2425 2796 4164
Size: 53 83 186 936 634 258 371 1368
Cuts at: 4164 5350 5732 6367 275
Size: 1186 382 635 1451
Fragments arranged by size:
1451 1368 1186 936 635 634 382 371
258 186 83 53
BsaWI w'CCGG_w
Cuts at: 2274 2856 3759 4000 5976 6374 6648 6795 7206
Size: 582 903 241 1976 398 274 147 411
Cuts at: 7206 2274
Size: 2611
Fragments arranged by size:
2611 1976 903 582 411 398 274 241
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4684 4714 1178
Size: 30 165 30 3281 30 4007
Fragments arranged by size:
4007 3281 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 3149 3440 1186
Size: 1408 555 291 5289
Fragments arranged by size:
5289 1408 555 291
BseYI C'CCAG_C
Cuts at: 2310 2391 3491 3645 4010 4167 4576 4736 4786
Size: 81 1100 154 365 157 409 160 50
Cuts at: 4786 6746 7242 2310
Size: 1960 496 2611
Fragments arranged by size:
2611 1960 1100 496 409 365 160 157
154 81 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2921 3694 4076 4091 4313 1163
Size: 573 1185 773 382 15 222 4393
Fragments arranged by size:
4393 1185 773 573 382 222 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2871 4081 4993 5754 6782 1540
Size: 275 186 870 1210 912 761 1028 2301
Fragments arranged by size:
2301 1210 1028 912 870 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2475 2496 2654 2709 2723 2856 3551 3852
Size: 210 21 158 55 14 133 695 301
Cuts at: 3852 4368 4650 5604 5689 6760 2265
Size: 516 282 954 85 1071 3048
Fragments arranged by size:
3048 1071 954 695 516 301 282 210
158 133 85 55 21 14
BsmI GAATG_Cn'
Cuts at: 2548 3585 5054 5147 2548
Size: 1037 1469 93 4944
Fragments arranged by size:
4944 1469 1037 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 2051
Size: 7543
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2385 2475 2496 2640 2654 2709
Size: 399 475 120 90 21 144 14 55
Cuts at: 2709 2723 2856 3091 3551 3567 3646 3774 3852
Size: 14 133 235 460 16 79 128 78
Cuts at: 3852 3990 4008 4368 4650 4716 4778 4786 4862
Size: 138 18 360 282 66 62 8 76
Cuts at: 4862 5604 5689 6760 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 460 399 360 282
235 144 138 133 128 120 90 85
79 78 76 66 62 55 21 18
16 14 14 8
BspEI T'CCGG_A
Cuts at: 2274 2856 3759 7206 2274
Size: 582 903 3447 2611
Fragments arranged by size:
3447 2611 903 582
BspHI T'CATG_A
Cuts at: 5432 7162 5432
Size: 1730 5813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2751 2796 2839 4420 4898 844
Size: 1907 45 43 1581 478 3489
Fragments arranged by size:
3489 1907 1581 478 45 43
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 2035 2572 5430 1394
Size: 429 189 23 537 2858 3507
Fragments arranged by size:
3507 2858 537 429 189 23
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 6038 6212 65
Size: 1183 1158 3632 174 1396
Fragments arranged by size:
3632 1396 1183 1158 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3273 3883 4000 4509 6187 6374 1700
Size: 110 1463 610 117 509 1678 187 2869
Fragments arranged by size:
2869 1678 1463 610 509 187 117 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7543
BssHII G'CGCG_C
Cuts at: 1925 2729 1925
Size: 804 6739
BssSI C'ACGA_G
Cuts at: 3386 4331 5603 6615 3386
Size: 945 1272 1012 4314
Fragments arranged by size:
4314 1272 1012 945
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2461 3172 794
Size: 1667 711 5165
Fragments arranged by size:
5165 1667 711
BstBI TT'CG_AA
Cuts at: 6347 6347
Size: 7543
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7543
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 4156 4679 2701
Size: 1455 523 5565
Fragments arranged by size:
5565 1455 523
BstYI r'GATC_y
Cuts at: 1972 2446 2941 3045 3663 4873 5240 5625 5642
Size: 474 495 104 618 1210 367 385 17
Cuts at: 5642 7094 7180 7192 7538 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1210 618 495 474 385 367
346 104 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4533 4533
Size: 7543
BtgI C'CryG_G
Cuts at: 513 1065 2004 2233 4539 513
Size: 552 939 229 2306 3517
Fragments arranged by size:
3517 2306 939 552 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3957 487
Size: 683 56 2731 4073
Fragments arranged by size:
4073 2731 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 3161 3711 5059 5853 5873 2305
Size: 856 550 1348 794 20 3975
Fragments arranged by size:
3975 1348 856 794 550 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7543
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7508
DraI TTT'AAA
Cuts at: 4847 4847
Size: 7543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6550 809
Size: 507 5234 1802
Fragments arranged by size:
5234 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2214 2376
Size: 54 1425 50 275 18 177 207 162
Cuts at: 2376 2502 2868 3295 3735 3972 4511 4990 5879
Size: 126 366 427 440 237 539 479 889
Cuts at: 5879 7508 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 539 479 440 427 366
275 237 207 177 162 126 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2868 4990 1537
Size: 275 1056 2122 4090
Fragments arranged by size:
4090 2122 1056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2432 3467 3785 3914 5473 1353
Size: 1079 1035 318 129 1559 3423
Fragments arranged by size:
3423 1559 1079 1035 318 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4202 4658 6130 6504 6650
Size: 1064 138 2546 67 456 1472 374 146
Cuts at: 6650 387
Size: 1280
Fragments arranged by size:
2546 1472 1280 1064 456 374 146 138
67
EcoICRI GAG'CTC
Cuts at: 2473 2494 2652 3549 4648 2473
Size: 21 158 897 1099 5368
Fragments arranged by size:
5368 1099 897 158 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2212 2471 2480 2498 2931 3173
Size: 393 497 83 259 9 18 433 242
Cuts at: 3173 3197 3320 3392 3767 3788 3845 4193 4259
Size: 24 123 72 375 21 57 348 66
Cuts at: 4259 5606 6203 6990 7258 1239
Size: 1347 597 787 268 1524
Fragments arranged by size:
1524 1347 787 597 497 433 393 375
348 268 259 242 123 83 72 66
57 24 21 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2366 2660 2846 3176 2366
Size: 294 186 330 6733
Fragments arranged by size:
6733 330 294 186
EcoO109I rG'GnC_Cy
Cuts at: 2200 2382 2636 2637 2905 2935 3169 3403 3691
Size: 182 254 1 268 30 234 234 288
Cuts at: 3691 4018 4338 4381 4483 4544 4675 4713 4729
Size: 327 320 43 102 61 131 38 16
Cuts at: 4729 4774 4775 4782 4783 4816 2200
Size: 45 1 7 1 33 4927
Fragments arranged by size:
4927 327 320 288 268 254 234 234
182 131 102 61 45 43 38 33
30 16 7 1 1 1
EcoRI G'AATT_C
Cuts at: 4852 5230 4852
Size: 378 7165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3772 4666 3772
Size: 894 6649
FspI TGC'GCA
Cuts at: 6049 6049
Size: 7543
HaeII r_GCGC'y
Cuts at: 1678 2170 2224 2398 2428 4031 6690 1678
Size: 492 54 174 30 1603 2659 2531
Fragments arranged by size:
2659 2531 1603 492 174 54 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2431 2463 3345 3377 3713
Size: 32 135 32 859 32 882 32 336
Cuts at: 3713 3745 6184 6216 6258 6290 1373
Size: 32 2439 32 42 32 2626
Fragments arranged by size:
2626 2439 882 859 336 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 4887 5133 669
Size: 988 281 826 2123 246 3079
Fragments arranged by size:
3079 2123 988 826 281 246
HindIII A'AGCT_T
Cuts at: 748 2269 748
Size: 1521 6022
HpaI GTT'AAC
Cuts at: 5133 5133
Size: 7543
KasI G'GCGC_C
Cuts at: 2166 2424 2166
Size: 258 7285
KpnI G_GTAC'C
Cuts at: 4868 4868
Size: 7543
MfeI C'AATT_G
Cuts at: 5142 5142
Size: 7543
MluI A'CGCG_T
Cuts at: 7419 7419
Size: 7543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6657 6841 1305
Size: 5352 184 2007
Fragments arranged by size:
5352 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5721
Fragments arranged by size:
5721 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3021 5501 5860 6019 518
Size: 648 1855 2480 359 159 2042
Fragments arranged by size:
2480 2042 1855 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2098 2461 2618 2873 3001 3836 4010
Size: 480 12 363 157 255 128 835 174
Cuts at: 4010 4051 4152 4171 4959 5636 6784 7029 1606
Size: 41 101 19 788 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 788 677 480 363 255
245 174 157 128 101 41 19 12
NaeI GCC'GGC
Cuts at: 3275 3275
Size: 7543
NarI GG'CG_CC
Cuts at: 2167 2425 2167
Size: 258 7285
NcoI C'CATG_G
Cuts at: 513 1065 2004 4539 513
Size: 552 939 2535 3517
Fragments arranged by size:
3517 2535 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7543
NgoMIV G'CCGG_C
Cuts at: 3273 3273
Size: 7543
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7543
NotI GC'GGCC_GC
Cuts at: 4990 4990
Size: 7543
NspI r_CATG'y
Cuts at: 1132 1229 2981 4378 4901 6446 1132
Size: 97 1752 1397 523 1545 2229
Fragments arranged by size:
2229 1752 1545 1397 523 97
PasI CC'CwG_GG
Cuts at: 1348 2842 4778 4813 1348
Size: 1494 1936 35 4078
Fragments arranged by size:
4078 1936 1494 35
PciI A'CATG_T
Cuts at: 6442 6442
Size: 7543
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3724 1108
Size: 2616 4927
PfoI T'CCnGG_A
Cuts at: 2475 2559 3336 2475
Size: 84 777 6682
Fragments arranged by size:
6682 777 84
PmeI GTTT'AAAC
Cuts at: 4847 4847
Size: 7543
PmlI CAC'GTG
Cuts at: 3853 4597 3853
Size: 744 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 5612 5644 7125 7157 4616
Size: 32 964 32 1481 32 5002
Fragments arranged by size:
5002 1481 964 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3691 4018 4544 3691
Size: 327 526 6690
Fragments arranged by size:
6690 526 327
PshAI GACnn'nnGTC
Cuts at: 1613 2063 4664 1613
Size: 450 2601 4492
Fragments arranged by size:
4492 2601 450
PsiI TTA'TAA
Cuts at: 5113 5113
Size: 7543
PspOMI G'GGCC_C
Cuts at: 2381 2636 3986 4712 4774 4782 2381
Size: 255 1350 726 62 8 5142
Fragments arranged by size:
5142 1350 726 255 62 8
PspXI vC'TCGA_Gb
Cuts at: 4342 4342
Size: 7543
PstI C_TGCA'G
Cuts at: 830 2046 2118 2145 2328 2466 3168 3378 3399
Size: 1216 72 27 183 138 702 210 21
Cuts at: 3399 3615 4035 4452 4459 4895 830
Size: 216 420 417 7 436 3478
Fragments arranged by size:
3478 1216 702 436 420 417 216 210
183 138 72 27 21 7
PvuI CG_AT'CG
Cuts at: 2001 4081 2001
Size: 2080 5463
PvuII CAG'CTG
Cuts at: 2086 2098 2461 4051 4152 4171 2086
Size: 12 363 1590 101 19 5458
Fragments arranged by size:
5458 1590 363 101 19 12
SacI G_AGCT'C
Cuts at: 2475 2496 2654 3551 4650 2475
Size: 21 158 897 1099 5368
Fragments arranged by size:
5368 1099 897 158 21
SalI G'TCGA_C
Cuts at: 1936 4885 1936
Size: 2949 4594
SanDI GG'GwC_CC
Cuts at: 4544 4544
Size: 7543
SapI GCTCTTCn'nnn_
Cuts at: 2432 3467 2432
Size: 1035 6508
SbfI CC_TGCA'GG
Cuts at: 4895 4895
Size: 7543
ScaI AGT'ACT
Cuts at: 5791 7503 5791
Size: 1712 5831
SfcI C'TryA_G
Cuts at: 826 1045 2042 2114 2141 2324 2462 3164 3374
Size: 219 997 72 27 183 138 702 210
Cuts at: 3374 3395 3611 4031 4448 4455 4891 6026 6707
Size: 21 216 420 417 7 436 1135 681
Cuts at: 6707 6898 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 702 681 436 420 417
219 216 210 191 183 138 72 27
21 7
SfoI GGC'GCC
Cuts at: 2168 2426 2168
Size: 258 7285
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7543
SgrAI Cr'CCGG_yG
Cuts at: 1700 3883 1700
Size: 2183 5360
SmaI CCC'GGG
Cuts at: 1872 2198 4772 4870 1872
Size: 326 2574 98 4545
Fragments arranged by size:
4545 2574 326 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 2714 2969 3245 4342 5647
Size: 197 926 714 57 255 276 1097 1305
Cuts at: 5647 6548 6810 820
Size: 901 262 1553
Fragments arranged by size:
1553 1305 1097 926 901 714 276 262
255 197 57
SnaBI TAC'GTA
Cuts at: 493 5334 493
Size: 4841 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7543
SphI G_CATG'C
Cuts at: 1132 2981 4378 4901 1132
Size: 1849 1397 523 3774
Fragments arranged by size:
3774 1849 1397 523
SspI AAT'ATT
Cuts at: 5 52 5467 5
Size: 47 5415 2081
Fragments arranged by size:
5415 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7543
StyI C'CwwG_G
Cuts at: 513 1065 2004 2961 4539 4678 4982 513
Size: 552 939 957 1578 139 304 3074
Fragments arranged by size:
3074 1578 957 939 552 304 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3129 3326 5570 5587 5740 5925
Size: 1255 1320 229 197 2244 17 153 185
Cuts at: 5925 6370 325
Size: 445 1498
Fragments arranged by size:
2244 1498 1320 1255 445 229 197 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3450 3936 5789
Size: 274 80 33 51 1443 1473 486 1853
Cuts at: 5789 7501 96
Size: 1712 138
Fragments arranged by size:
1853 1712 1473 1443 486 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7503 7535 7503
Size: 32 7511
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7543
XbaI T'CTAG_A
Cuts at: 4879 4879
Size: 7543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3023 3515 4744 3023
Size: 492 1229 5822
Fragments arranged by size:
5822 1229 492
XhoI C'TCGA_G
Cuts at: 1943 2969 4342 1943
Size: 1026 1373 5144
Fragments arranged by size:
5144 1373 1026
XmaI C'CCGG_G
Cuts at: 1870 2196 4770 4868 1870
Size: 326 2574 98 4545
Fragments arranged by size:
4545 2574 326 98
XmnI GAAnn'nnTTC
Cuts at: 5672 5672
Size: 7543
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2797 5351 276
Size: 53 83 186 936 1263 2554 2468
Fragments arranged by size:
2554 2468 1263 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII
SacII SexAI SfiI SrfI SwaI