(Circular) (Six-base) MAPSORT of: pFN21AA0130.seq Check: 4661 from: 1 to: 7672 pFN21AA0130 7672 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5482 278 Size: 53 83 186 936 3946 2468 Fragments arranged by size: 3946 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4176 4227 5015 1937 Size: 2239 51 788 4594 Fragments arranged by size: 4594 2239 788 51 Acc65I G'GTAC_C Cuts at: 4993 4993 Size: 7672 AclI AA'CG_TT Cuts at: 1563 5799 6172 1563 Size: 4236 373 3063 Fragments arranged by size: 4236 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2216 2474 3050 4283 5735 7119 7387 Size: 410 174 258 576 1233 1452 1384 268 Cuts at: 7387 1632 Size: 1917 Fragments arranged by size: 1917 1452 1384 1233 576 410 268 258 174 AfeI AGC'GCT Cuts at: 2054 3065 2054 Size: 1011 6661 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7475 AflIII A'CryG_T Cuts at: 1203 2310 6571 7548 1203 Size: 1107 4261 977 1327 Fragments arranged by size: 4261 1327 1107 977 AgeI A'CCGG_T Cuts at: 6503 6503 Size: 7672 AhdI GACnn_n'nnGTC Cuts at: 6401 6401 Size: 7672 AleI CACnn'nnGTG Cuts at: 1166 3662 4076 4856 1166 Size: 2496 414 780 3982 Fragments arranged by size: 3982 2496 780 414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2368 4850 1184 Size: 1184 2482 4006 Fragments arranged by size: 4006 2482 1184 AlwNI CAG_nnn'CTG Cuts at: 2417 2462 2497 2791 3563 5415 6987 2417 Size: 45 35 294 772 1852 1572 3102 Fragments arranged by size: 3102 1852 1572 772 294 45 35 ApaI G_GGCC'C Cuts at: 2533 3475 2533 Size: 942 6730 ApaLI G'TGCA_C Cuts at: 5729 6885 5729 Size: 1156 6516 ApoI r'AATT_y Cuts at: 1493 2090 3404 3422 4981 5207 5359 7468 1493 Size: 597 1314 18 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 1314 597 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 6226 160 Size: 872 5194 1606 Fragments arranged by size: 5194 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7672 AvaI C'yCGr_G Cuts at: 1870 1943 2271 2753 2894 4417 4997 1870 Size: 73 328 482 141 1523 580 4545 Fragments arranged by size: 4545 1523 580 482 328 141 73 BamHI G'GATC_C Cuts at: 5002 5369 5002 Size: 367 7305 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2660 2759 3568 4993 6448 Size: 325 217 627 873 99 809 1425 1455 Cuts at: 6448 618 Size: 1842 Fragments arranged by size: 1842 1455 1425 873 809 627 325 217 99 BanII G_rGCy'C Cuts at: 1391 2374 2533 2883 2913 3249 3475 4592 4625 Size: 983 159 350 30 336 226 1117 33 Cuts at: 4625 4991 1391 Size: 366 4072 Fragments arranged by size: 4072 1117 983 366 350 336 226 159 33 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4524 928 Size: 971 2625 4076 Fragments arranged by size: 4076 2625 971 BbvCI CC'TCA_GC Cuts at: 2510 3564 4922 2510 Size: 1054 1358 5260 Fragments arranged by size: 5260 1358 1054 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4558 4592 5863 5897 1903 Size: 34 2621 34 1271 34 3678 Fragments arranged by size: 3678 2621 1271 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5564 6774 5564 Size: 1210 6462 BclI T'GATC_A Cuts at: 1548 3996 1548 Size: 2448 5224 BfrBI ATG'CAT Cuts at: 2355 2355 Size: 7672 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4439 6283 136 Size: 107 122 71 631 605 2767 1844 1525 Fragments arranged by size: 2767 1844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2856 7667 2856 Size: 4811 2861 BlpI GC'TnA_GC Cuts at: 3153 4586 5089 3153 Size: 1433 503 5736 Fragments arranged by size: 5736 1433 503 Bme1580I G_kGCm'C Cuts at: 1790 2533 2762 3475 3573 4424 4456 5733 6889 Size: 743 229 713 98 851 32 1277 1156 Cuts at: 6889 1790 Size: 2573 Fragments arranged by size: 2573 1277 1156 851 743 713 229 98 32 BmgBI CAC'GTC Cuts at: 1318 3886 4033 1318 Size: 2568 147 4957 Fragments arranged by size: 4957 2568 147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4762 5403 6361 Size: 416 53 83 399 24 3345 641 958 Cuts at: 6361 442 Size: 1753 Fragments arranged by size: 3345 1753 958 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7672 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 7672 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2077 2439 2462 2519 2873 4034 4181 4829 Size: 838 362 23 57 354 1161 147 648 Cuts at: 4829 6332 1239 Size: 1503 2579 Fragments arranged by size: 2579 1503 1161 838 648 362 354 147 57 23 Bpu10I CC'TnA_GC Cuts at: 2510 3564 4922 2510 Size: 1054 1358 5260 Fragments arranged by size: 5260 1358 1054 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2897 2921 3251 5797 6662 6960 2897 Size: 24 330 2546 865 298 3609 Fragments arranged by size: 3609 2546 865 330 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3575 4749 6335 882 Size: 617 2076 1174 1586 2219 Fragments arranged by size: 2219 2076 1586 1174 617 BsaAI yAC'GTr Cuts at: 493 1204 5463 493 Size: 711 4259 2702 Fragments arranged by size: 4259 2702 711 BsaBI GATnn'nnATC Cuts at: 7666 7666 Size: 7672 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4224 4487 5479 5861 Size: 53 83 186 936 2691 263 992 382 Cuts at: 5861 6496 275 Size: 635 1451 Fragments arranged by size: 2691 1451 992 936 635 382 263 186 83 53 BsaWI w'CCGG_w Cuts at: 2637 3298 3888 6105 6503 6777 6924 7335 2637 Size: 661 590 2217 398 274 147 411 2974 Fragments arranged by size: 2974 2217 661 590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 3552 3582 4007 Size: 30 165 30 1497 30 622 30 425 Cuts at: 4007 4037 4169 4199 1178 Size: 30 132 30 4651 Fragments arranged by size: 4651 1497 622 425 165 132 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 2921 3623 3685 4378 4459 4720 4805 Size: 1384 351 702 62 693 81 261 85 Cuts at: 4805 1186 Size: 4053 Fragments arranged by size: 4053 1384 702 693 351 261 85 81 62 BseYI C'CCAG_C Cuts at: 2462 3183 3310 3895 4170 4354 6875 7371 2462 Size: 721 127 585 275 184 2521 496 2763 Fragments arranged by size: 2763 2521 721 585 496 275 184 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 2522 3734 4196 4580 4796 4950 Size: 573 653 133 1212 462 384 216 154 Cuts at: 4950 1163 Size: 3885 Fragments arranged by size: 3885 1212 653 573 462 384 216 154 133 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5122 5883 6911 1540 Size: 275 186 3121 761 1028 2301 Fragments arranged by size: 3121 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2913 3177 4284 4592 5733 5818 6889 2913 Size: 264 1107 308 1141 85 1071 3696 Fragments arranged by size: 3696 1141 1107 1071 308 264 85 BsmI GAATG_Cn' Cuts at: 2357 2584 3208 5183 5276 2357 Size: 227 624 1975 93 4753 Fragments arranged by size: 4753 1975 624 227 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2774 4262 2774 Size: 1488 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 2533 2762 2883 2913 3177 3249 Size: 399 584 159 229 121 30 264 72 Cuts at: 3249 3475 3573 4284 4424 4456 4592 4625 4991 Size: 226 98 711 140 32 136 33 366 Cuts at: 4991 5733 5818 6889 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 711 584 399 366 264 229 226 159 140 136 121 98 85 72 33 32 30 BspEI T'CCGG_A Cuts at: 2637 3888 7335 2637 Size: 1251 3447 2974 Fragments arranged by size: 3447 2974 1251 BspHI T'CATG_A Cuts at: 3999 5561 7291 3999 Size: 1562 1730 4380 Fragments arranged by size: 4380 1730 1562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3605 4110 5027 844 Size: 2761 505 917 3489 Fragments arranged by size: 3489 2761 917 505 BsrBI CCG'CTC Cuts at: 1394 1823 2425 4185 5559 1394 Size: 429 602 1760 1374 3507 Fragments arranged by size: 3507 1760 1374 602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2291 4959 6167 6341 65 Size: 1183 1043 2668 1208 174 1396 Fragments arranged by size: 2668 1396 1208 1183 1043 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2447 2720 3457 6316 6503 1700 Size: 110 637 273 737 2859 187 2869 Fragments arranged by size: 2869 2859 737 637 273 187 110 BsrGI T'GTAC_A Cuts at: 96 3080 96 Size: 2984 4688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7672 BssSI C'ACGA_G Cuts at: 5732 6744 5732 Size: 1012 6660 BstAPI GCAn_nnn'nTGC Cuts at: 794 2417 4288 4843 794 Size: 1623 1871 555 3623 Fragments arranged by size: 3623 1871 1623 555 BstBI TT'CG_AA Cuts at: 4568 6476 4568 Size: 1908 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7672 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3104 3524 3664 4078 4159 2011 Size: 1093 420 140 414 81 5524 Fragments arranged by size: 5524 1093 420 414 140 81 BstYI r'GATC_y Cuts at: 1972 2607 2856 3618 5002 5369 5754 5771 7223 Size: 635 249 762 1384 367 385 17 1452 Cuts at: 7223 7309 7321 7667 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1384 762 635 385 367 346 249 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3606 3606 Size: 7672 BtgI C'CryG_G Cuts at: 513 1065 4822 513 Size: 552 3757 3363 Fragments arranged by size: 3757 3363 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2527 487 Size: 683 56 1301 5632 Fragments arranged by size: 5632 1301 683 56 BtsI GCAGTG_nn' Cuts at: 2660 4302 4422 4784 5188 5982 6002 2660 Size: 1642 120 362 404 794 20 4330 Fragments arranged by size: 4330 1642 794 404 362 120 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7672 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2934 2969 529 Size: 35 2370 35 5232 Fragments arranged by size: 5232 2370 35 35 DraI TTT'AAA Cuts at: 4976 4976 Size: 7672 DraIII CAC_nnn'GTG Cuts at: 2743 2743 Size: 7672 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2282 6679 809 Size: 507 966 4397 1802 Fragments arranged by size: 4397 1802 966 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4440 4707 4819 Size: 54 1425 50 275 18 2610 267 112 Cuts at: 4819 4908 5119 6008 7637 8 Size: 89 211 889 1629 43 Fragments arranged by size: 2610 1629 1425 889 275 267 211 112 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5119 1537 Size: 275 3307 4090 Fragments arranged by size: 4090 3307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2236 2569 3335 4499 4547 5602 1353 Size: 883 333 766 1164 48 1055 3423 Fragments arranged by size: 3423 1164 1055 883 766 333 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3257 3635 3923 6259 6633 6779 Size: 1064 138 1668 378 288 2336 374 146 Cuts at: 6779 387 Size: 1280 Fragments arranged by size: 2336 1668 1280 1064 378 374 288 146 138 EcoICRI GAG'CTC Cuts at: 2911 4590 2911 Size: 1679 5993 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2077 2216 2439 2462 2474 2519 Size: 393 410 35 139 223 23 12 45 Cuts at: 2519 2873 3050 4034 4181 4283 4829 5735 6332 Size: 354 177 984 147 102 546 906 597 Cuts at: 6332 7119 7387 1239 Size: 787 268 1524 Fragments arranged by size: 1524 984 906 787 597 546 410 393 354 268 223 177 147 139 102 45 35 23 12 EcoNI CCTnn'n_nnAGG Cuts at: 2753 4250 4811 2753 Size: 1497 561 5614 Fragments arranged by size: 5614 1497 561 EcoO109I rG'GnC_Cy Cuts at: 2275 2530 3472 3790 4687 2275 Size: 255 942 318 897 5260 Fragments arranged by size: 5260 942 897 318 255 EcoRI G'AATT_C Cuts at: 3422 4981 5359 3422 Size: 1559 378 5735 Fragments arranged by size: 5735 1559 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2110 2740 2110 Size: 630 7042 FspI TGC'GCA Cuts at: 6178 6178 Size: 7672 HaeII r_GCGC'y Cuts at: 1678 2056 2385 2497 3067 6819 1678 Size: 378 329 112 570 3752 2531 Fragments arranged by size: 3752 2531 570 378 329 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2771 2803 4169 4201 6313 Size: 32 135 32 1199 32 1366 32 2112 Cuts at: 6313 6345 6387 6419 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2112 1366 1199 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 3007 3139 4678 5016 5262 Size: 988 281 79 990 132 1539 338 246 Cuts at: 5262 669 Size: 3079 Fragments arranged by size: 3079 1539 990 988 338 281 246 132 79 HindIII A'AGCT_T Cuts at: 748 2865 748 Size: 2117 5555 HpaI GTT'AAC Cuts at: 5262 5262 Size: 7672 KpnI G_GTAC'C Cuts at: 4997 4997 Size: 7672 MfeI C'AATT_G Cuts at: 5271 5271 Size: 7672 MluI A'CGCG_T Cuts at: 7548 7548 Size: 7672 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3027 4842 6786 6970 1305 Size: 1722 1815 1944 184 2007 Fragments arranged by size: 2007 1944 1815 1722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4709 4910 10 Size: 54 1425 343 2877 201 2772 Fragments arranged by size: 2877 2772 1425 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2309 3662 4076 4856 5630 5989 Size: 648 843 300 1353 414 780 774 359 Cuts at: 5989 6148 518 Size: 159 2042 Fragments arranged by size: 2042 1353 843 780 774 648 414 359 300 159 MspA1I CmG'CkG Cuts at: 1606 2462 2803 3229 3940 4754 5088 5765 6913 Size: 856 341 426 711 814 334 677 1148 Cuts at: 6913 7158 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 856 814 711 677 426 341 334 245 NaeI GCC'GGC Cuts at: 2449 2449 Size: 7672 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7120 NdeI CA'TA_TG Cuts at: 387 387 Size: 7672 NgoMIV G'CCGG_C Cuts at: 2447 2447 Size: 7672 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7672 NotI GC'GGCC_GC Cuts at: 5119 5119 Size: 7672 NsiI A_TGCA'T Cuts at: 2357 2357 Size: 7672 NspI r_CATG'y Cuts at: 1132 1229 2314 2702 2824 3331 3604 4024 4333 Size: 97 1085 388 122 507 273 420 309 Cuts at: 4333 4684 5030 6575 1132 Size: 351 346 1545 2229 Fragments arranged by size: 2229 1545 1085 507 420 388 351 346 309 273 122 97 PasI CC'CwG_GG Cuts at: 1348 4933 1348 Size: 3585 4087 PciI A'CATG_T Cuts at: 2310 6571 2310 Size: 4261 3411 PflMI CCAn_nnn'nTGG Cuts at: 1108 2462 1108 Size: 1354 6318 PfoI T'CCnGG_A Cuts at: 2850 3335 4011 2850 Size: 485 676 6511 Fragments arranged by size: 6511 676 485 PmeI GTTT'AAAC Cuts at: 4976 4976 Size: 7672 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5741 5773 7254 7286 5741 Size: 32 1481 32 6127 Fragments arranged by size: 6127 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 3790 4687 2275 Size: 1515 897 5260 Fragments arranged by size: 5260 1515 897 PshAI GACnn'nnGTC Cuts at: 1613 4346 4486 1613 Size: 2733 140 4799 Fragments arranged by size: 4799 2733 140 PsiI TTA'TAA Cuts at: 5242 5242 Size: 7672 PspOMI G'GGCC_C Cuts at: 2529 3471 2529 Size: 942 6730 PspXI vC'TCGA_Gb Cuts at: 2753 2753 Size: 7672 PstI C_TGCA'G Cuts at: 830 2422 3927 3975 4041 4839 5024 830 Size: 1592 1505 48 66 798 185 3478 Fragments arranged by size: 3478 1592 1505 798 185 66 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7672 PvuII CAG'CTG Cuts at: 2803 3229 3940 2803 Size: 426 711 6535 Fragments arranged by size: 6535 711 426 RsrII CG'GwC_CG Cuts at: 3166 3166 Size: 7672 SacI G_AGCT'C Cuts at: 2913 4592 2913 Size: 1679 5993 SalI G'TCGA_C Cuts at: 1936 5014 1936 Size: 3078 4594 SanDI GG'GwC_CC Cuts at: 4687 4687 Size: 7672 SbfI CC_TGCA'GG Cuts at: 5024 5024 Size: 7672 ScaI AGT'ACT Cuts at: 2972 5920 7632 2972 Size: 2948 1712 3012 Fragments arranged by size: 3012 2948 1712 SfcI C'TryA_G Cuts at: 826 1045 2418 3923 3971 4037 4835 5020 6155 Size: 219 1373 1505 48 66 798 185 1135 Cuts at: 6155 6836 7027 826 Size: 681 191 1471 Fragments arranged by size: 1505 1471 1373 1135 798 681 219 191 185 66 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7672 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7672 SmaI CCC'GGG Cuts at: 1872 4419 4999 1872 Size: 2547 580 4545 Fragments arranged by size: 4545 2547 580 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 2912 2936 3266 5776 6677 Size: 197 926 810 159 24 330 2510 901 Cuts at: 6677 6939 820 Size: 262 1553 Fragments arranged by size: 2510 1553 926 901 810 330 262 197 159 24 SnaBI TAC'GTA Cuts at: 493 5463 493 Size: 4970 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7672 SphI G_CATG'C Cuts at: 1132 2702 2824 3604 5030 1132 Size: 1570 122 780 1426 3774 Fragments arranged by size: 3774 1570 1426 780 122 SspI AAT'ATT Cuts at: 5 52 5596 5 Size: 47 5544 2081 Fragments arranged by size: 5544 2081 47 StuI AGG'CCT Cuts at: 1517 2561 3734 1517 Size: 1044 1173 5455 Fragments arranged by size: 5455 1173 1044 StyI C'CwwG_G Cuts at: 513 1065 4152 4896 5111 513 Size: 552 3087 744 215 3074 Fragments arranged by size: 3087 3074 744 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3183 4647 4781 5699 5716 5869 6054 Size: 1255 1603 1464 134 918 17 153 185 Cuts at: 6054 6499 325 Size: 445 1498 Fragments arranged by size: 1603 1498 1464 1255 918 445 185 153 134 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 3080 5918 Size: 274 80 33 51 1443 993 110 2838 Cuts at: 5918 7630 96 Size: 1712 138 Fragments arranged by size: 2838 1712 1443 993 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2832 2864 2900 2932 3317 3349 3552 3584 4007 Size: 32 36 32 385 32 203 32 423 Cuts at: 4007 4039 7632 7664 2832 Size: 32 3593 32 2840 Fragments arranged by size: 3593 2840 423 385 203 36 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2785 1316 Size: 1469 6203 XbaI T'CTAG_A Cuts at: 5008 5008 Size: 7672 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4321 4367 4897 4903 4321 Size: 46 530 6 7090 Fragments arranged by size: 7090 530 46 6 XhoI C'TCGA_G Cuts at: 1943 2753 1943 Size: 810 6862 XmaI C'CCGG_G Cuts at: 1870 4417 4997 1870 Size: 2547 580 4545 Fragments arranged by size: 4545 2547 580 XmnI GAAnn'nnTTC Cuts at: 2090 3392 3422 5801 2090 Size: 1302 30 2379 3961 Fragments arranged by size: 3961 2379 1302 30 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5480 276 Size: 53 83 186 936 3946 2468 Fragments arranged by size: 3946 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PsrI SacII SapI SexAI SfiI SfoI SrfI SwaI