(Circular) (Six-base) MAPSORT of: pFN21AA0128.seq Check: 6582 from: 1 to: 5986 pFN21AA0128 5986 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2709 2709 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2510 2756 2941 3137 3281 4049 5433 5701 Size: 878 246 185 196 144 768 1384 268 Cuts at: 5701 1632 Size: 1917 Fragments arranged by size: 1917 1384 878 768 268 246 196 185 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2068 4885 5862 1203 Size: 865 2817 977 1327 Fragments arranged by size: 2817 1327 977 865 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2174 Size: 32 5954 AlwNI CAG_nnn'CTG Cuts at: 3259 3729 5301 3259 Size: 470 1572 3944 Fragments arranged by size: 3944 1572 470 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2201 2363 3295 3521 3673 5782 1493 Size: 708 162 932 226 152 2109 1697 Fragments arranged by size: 2109 1697 932 708 226 162 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 3311 1870 Size: 73 1368 4545 Fragments arranged by size: 4545 1368 73 AvrII C'CTAG_G Cuts at: 2998 2998 Size: 5986 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 4762 618 Size: 325 217 627 1520 1455 1842 Fragments arranged by size: 1842 1520 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3075 3236 3305 1391 Size: 1684 161 69 4072 Fragments arranged by size: 4072 1684 161 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2781 3878 5088 2781 Size: 1097 1210 3679 Fragments arranged by size: 3679 1210 1097 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2637 5981 2637 Size: 3344 2642 BlpI GC'TnA_GC Cuts at: 3213 3403 3213 Size: 190 5796 Bme1580I G_kGCm'C Cuts at: 1790 2783 4047 5203 1790 Size: 993 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 993 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2933 2993 3170 3206 3264 4646 1239 Size: 992 702 60 177 36 58 1382 2579 Fragments arranged by size: 2579 1382 992 702 177 60 58 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4111 4976 5274 4111 Size: 865 298 4823 Fragments arranged by size: 4823 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 1178 Size: 30 165 30 1020 30 4711 Fragments arranged by size: 4711 1020 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2930 3038 1186 Size: 1744 108 4134 Fragments arranged by size: 4134 1744 108 BseYI C'CCAG_C Cuts at: 5189 5685 5189 Size: 496 5490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 3080 3110 1163 Size: 573 1071 273 30 4039 Fragments arranged by size: 4039 1071 573 273 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2301 3436 4197 5225 1540 Size: 275 186 15 285 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 285 275 186 15 BsiHKAI G_wGCw'C Cuts at: 3075 4047 4132 5203 3075 Size: 972 85 1071 3858 Fragments arranged by size: 3858 1071 972 85 BsmI GAATG_Cn' Cuts at: 3190 3497 3590 3190 Size: 307 93 5586 Fragments arranged by size: 5586 307 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2783 3075 3236 3305 4047 4132 5203 Size: 399 993 292 161 69 742 85 1071 Cuts at: 5203 1391 Size: 2174 Fragments arranged by size: 2174 1071 993 742 399 292 161 85 69 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 2709 2779 3341 844 Size: 1556 309 70 562 3489 Fragments arranged by size: 3489 1556 562 309 70 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2466 2550 4481 4655 65 Size: 1183 1218 84 1931 174 1396 Fragments arranged by size: 1931 1396 1218 1183 174 84 BsrFI r'CCGG_y Cuts at: 1700 1810 4630 4817 1700 Size: 110 2820 187 2869 Fragments arranged by size: 2869 2820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 3098 4046 5058 3098 Size: 948 1012 4026 Fragments arranged by size: 4026 1012 948 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 2205 2573 4790 2205 Size: 368 2217 3401 Fragments arranged by size: 3401 2217 368 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2179 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2409 2637 2857 3316 3683 4068 4085 5537 Size: 437 228 220 459 367 385 17 1452 Cuts at: 5537 5623 5635 5981 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 459 437 385 367 346 228 220 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2475 513 Size: 552 939 471 4024 Fragments arranged by size: 4024 939 552 471 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2806 3502 4296 4316 2806 Size: 696 794 20 4476 Fragments arranged by size: 4476 794 696 20 ClaI AT'CG_AT Cuts at: 1554 2370 1554 Size: 816 5170 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951 Size: 54 1425 50 275 18 1603 889 1629 Cuts at: 5951 8 Size: 43 Fragments arranged by size: 1629 1603 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2410 2570 3015 3257 3916 1353 Size: 1041 16 160 445 242 659 3423 Fragments arranged by size: 3423 1041 659 445 242 160 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3073 3073 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2510 2756 2933 2941 2993 3137 Size: 393 599 279 246 177 8 52 144 Cuts at: 3137 3170 3206 3264 3281 4049 4646 5433 5701 Size: 33 36 58 17 768 597 787 268 Cuts at: 5701 1239 Size: 1524 Fragments arranged by size: 1524 787 768 599 597 393 279 268 246 177 144 58 52 36 33 17 8 EcoRI G'AATT_C Cuts at: 2363 3295 3673 2363 Size: 932 378 4676 Fragments arranged by size: 4676 932 378 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2622 2648 2654 2680 4627 Size: 32 135 32 1050 26 6 26 1947 Cuts at: 4627 4659 4701 4733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1947 1050 135 42 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 2848 3330 3576 669 Size: 988 281 910 482 246 3079 Fragments arranged by size: 3079 988 910 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4164 Fragments arranged by size: 4164 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3944 4303 4462 518 Size: 648 1011 1767 359 159 2042 Fragments arranged by size: 2042 1767 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2904 3402 4079 5227 5472 1606 Size: 492 806 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 806 677 498 492 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 2974 387 Size: 2587 3399 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2072 3344 4889 1132 Size: 97 843 1272 1545 2229 Fragments arranged by size: 2229 1545 1272 843 97 PasI CC'CwG_GG Cuts at: 1348 3229 1348 Size: 1881 4105 PciI A'CATG_T Cuts at: 2068 4885 2068 Size: 2817 3169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 2397 3338 830 Size: 1567 941 3478 Fragments arranged by size: 3478 1567 941 PvuI CG_AT'CG Cuts at: 2001 2301 2001 Size: 300 5686 PvuII CAG'CTG Cuts at: 2098 2098 Size: 5986 SacI G_AGCT'C Cuts at: 3075 3075 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SapI GCTCTTCn'nnn_ Cuts at: 2394 2394 Size: 5986 SbfI CC_TGCA'GG Cuts at: 2397 3338 2397 Size: 941 5045 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2393 2652 3334 4469 5150 5341 826 Size: 219 1348 259 682 1135 681 191 1471 Fragments arranged by size: 1471 1348 1135 682 681 259 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4090 4991 5253 820 Size: 197 926 2147 901 262 1553 Fragments arranged by size: 2147 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 2387 1517 Size: 870 5116 StyI C'CwwG_G Cuts at: 513 1065 2004 2774 2998 3425 513 Size: 552 939 770 224 427 3074 Fragments arranged by size: 3074 939 770 552 427 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2030 2237 4013 4030 4183 4368 Size: 1255 444 6 207 1776 17 153 185 Cuts at: 4368 4813 325 Size: 445 1498 Fragments arranged by size: 1776 1498 1255 445 444 207 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 2993 3188 4115 2993 Size: 195 927 4864 Fragments arranged by size: 4864 927 195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI