(Circular) (Six-base) MAPSORT of: pFN21AA0121.seq Check: 1259 from: 1 to: 5575 pFN21AA0121 5575 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3638 5022 5290 1632 Size: 2006 1384 268 1917 Fragments arranged by size: 2006 1917 1384 268 AfeI AGC'GCT Cuts at: 2605 2605 Size: 5575 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2745 4474 5451 1203 Size: 1542 1729 977 1327 Fragments arranged by size: 1729 1542 1327 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2474 2506 2474 Size: 32 5543 AlwNI CAG_nnn'CTG Cuts at: 2107 2562 3318 4890 2107 Size: 455 756 1572 2792 Fragments arranged by size: 2792 1572 756 455 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2884 3110 3262 5371 1493 Size: 1391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1391 226 152 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2295 2464 2573 2675 2900 1870 Size: 73 352 169 109 102 225 4545 Fragments arranged by size: 4545 352 225 169 109 102 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2516 2683 2896 4351 618 Size: 325 217 627 729 167 213 1455 1842 Fragments arranged by size: 1842 1455 729 627 325 217 213 167 BanII G_rGCy'C Cuts at: 1391 2676 2711 2894 1391 Size: 1285 35 183 4072 Fragments arranged by size: 4072 1285 183 35 BbeI G_GCGC'C Cuts at: 2520 2520 Size: 5575 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BbvCI CC'TCA_GC Cuts at: 2126 2126 Size: 5575 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2299 2333 2708 2742 3766 3800 1903 Size: 34 362 34 375 34 1024 34 3678 Fragments arranged by size: 3678 1024 375 362 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 2496 1548 Size: 948 4627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2352 4186 136 Size: 107 122 71 631 605 680 1834 1525 Fragments arranged by size: 1834 1525 680 631 605 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 3636 4792 1790 Size: 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 BmgBI CAC'GTC Cuts at: 1318 2224 1318 Size: 906 4669 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3306 4264 442 Size: 416 53 83 399 24 1889 958 1753 Fragments arranged by size: 1889 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2738 4235 1239 Size: 1172 327 1497 2579 Fragments arranged by size: 2579 1497 1172 327 Bpu10I CC'TnA_GC Cuts at: 2126 2126 Size: 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2283 2875 4238 882 Size: 617 784 592 1363 2219 Fragments arranged by size: 2219 1363 784 617 592 BsaAI yAC'GTr Cuts at: 493 1204 2748 3366 493 Size: 711 1544 618 2702 Fragments arranged by size: 2702 1544 711 618 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2427 2517 3382 Size: 53 83 186 936 479 415 90 865 Cuts at: 3382 3764 4399 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 865 635 479 415 382 186 90 83 53 BsaWI w'CCGG_w Cuts at: 2589 4008 4406 4680 4827 5238 2589 Size: 1419 398 274 147 411 2926 Fragments arranged by size: 2926 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2131 2161 2210 2240 2474 Size: 30 165 30 728 30 49 30 234 Cuts at: 2474 2504 2840 2870 1178 Size: 30 336 30 3883 Fragments arranged by size: 3883 728 336 234 165 49 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2648 1186 Size: 1462 4113 BseYI C'CCAG_C Cuts at: 2162 2378 4778 5274 2162 Size: 216 2400 496 2463 Fragments arranged by size: 2463 2400 496 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 1163 Size: 573 597 4405 Fragments arranged by size: 4405 597 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3636 3721 4792 3636 Size: 85 1071 4419 Fragments arranged by size: 4419 1071 85 BsmI GAATG_Cn' Cuts at: 2103 3086 3179 2103 Size: 983 93 4499 Fragments arranged by size: 4499 983 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2230 2003 Size: 227 5348 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 2711 2894 3636 3721 4792 1391 Size: 399 886 35 183 742 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 399 183 85 35 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2302 2473 3462 1394 Size: 429 479 171 989 3507 Fragments arranged by size: 3507 989 479 429 171 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2141 2441 2484 2680 4219 4406 Size: 110 300 31 300 43 196 1539 187 Cuts at: 4406 1700 Size: 2869 Fragments arranged by size: 2869 1539 300 300 196 187 110 43 31 BsrGI T'GTAC_A Cuts at: 96 2364 96 Size: 2268 3307 BssHII G'CGCG_C Cuts at: 1925 2323 1925 Size: 398 5177 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 2107 794 Size: 1313 4262 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstXI CCAn_nnnn'nTGG Cuts at: 2737 2737 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570 Size: 933 367 385 17 1452 86 12 346 Cuts at: 5570 1972 Size: 1977 Fragments arranged by size: 1977 1452 933 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2625 2712 513 Size: 552 939 621 87 3376 Fragments arranged by size: 3376 939 621 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2533 487 Size: 683 56 1307 3529 Fragments arranged by size: 3529 1307 683 56 BtsI GCAGTG_nn' Cuts at: 2539 3091 3885 3905 2539 Size: 552 794 20 4209 Fragments arranged by size: 4209 794 552 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2766 3022 3911 Size: 54 1425 50 275 18 936 256 889 Cuts at: 3911 5540 8 Size: 1629 43 Fragments arranged by size: 1629 1425 936 889 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3505 1353 Size: 2152 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2788 4162 4536 4682 387 Size: 1064 138 1199 1374 374 146 1280 Fragments arranged by size: 1374 1280 1199 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2738 3638 4235 5022 5290 1239 Size: 393 779 327 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 779 597 393 327 268 EcoNI CCTnn'n_nnAGG Cuts at: 2806 2806 Size: 5575 EcoO109I rG'GnC_Cy Cuts at: 2200 2200 Size: 5575 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 2520 2607 4722 1678 Size: 842 87 2115 2531 Fragments arranged by size: 2531 2115 842 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2210 2242 2765 Size: 32 135 32 440 32 166 32 523 Cuts at: 2765 2797 4216 4248 4290 4322 1373 Size: 32 1419 32 42 32 2626 Fragments arranged by size: 2626 1419 523 440 166 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2919 3165 669 Size: 988 281 981 246 3079 Fragments arranged by size: 3079 988 981 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KasI G'GCGC_C Cuts at: 2516 2516 Size: 5575 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 4873 1305 Size: 3384 184 2007 Fragments arranged by size: 3384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3753 Fragments arranged by size: 3753 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 3533 3892 4051 518 Size: 648 889 1478 359 159 2042 Fragments arranged by size: 2042 1478 889 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2559 2991 3668 4816 5061 1606 Size: 953 432 677 1148 245 2120 Fragments arranged by size: 2120 1148 953 677 432 245 NaeI GCC'GGC Cuts at: 2443 2682 2443 Size: 239 5336 NarI GG'CG_CC Cuts at: 2517 2517 Size: 5575 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NgoMIV G'CCGG_C Cuts at: 2441 2680 2441 Size: 239 5336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2054 2933 4478 1132 Size: 97 825 879 1545 2229 Fragments arranged by size: 2229 1545 879 825 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PmlI CAC'GTG Cuts at: 2748 2748 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2474 2506 3644 3676 5157 5189 2474 Size: 32 1138 32 1481 32 2860 Fragments arranged by size: 2860 1481 1138 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2200 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PstI C_TGCA'G Cuts at: 830 2927 830 Size: 2097 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2923 4058 4739 4930 826 Size: 219 1878 1135 681 191 1471 Fragments arranged by size: 1878 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2518 2518 Size: 5575 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2297 2466 2575 2902 1872 Size: 425 169 109 327 4545 Fragments arranged by size: 4545 425 327 169 109 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2054 2933 1132 Size: 922 879 3774 Fragments arranged by size: 3774 922 879 SrfI GCCC'GGGC Cuts at: 2575 2575 Size: 5575 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5575 StyI C'CwwG_G Cuts at: 513 1065 2004 2383 2769 3014 513 Size: 552 939 379 386 245 3074 Fragments arranged by size: 3074 939 552 386 379 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 2729 3602 3619 3772 3957 4402 Size: 1255 1121 28 873 17 153 185 445 Cuts at: 4402 325 Size: 1498 Fragments arranged by size: 1498 1255 1121 873 445 185 153 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2364 3821 5533 Size: 274 80 33 51 1443 387 1457 1712 Cuts at: 5533 96 Size: 138 Fragments arranged by size: 1712 1457 1443 387 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 2840 2872 5535 5567 2131 Size: 32 677 32 2663 32 2139 Fragments arranged by size: 2663 2139 677 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2222 1316 Size: 906 4669 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2419 2386 Size: 33 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2295 2464 2573 2900 1870 Size: 425 169 109 327 4545 Fragments arranged by size: 4545 425 327 169 109 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SwaI