Restriction map for
pFN21AA0116
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 355 GTCGAC GT^[AC][GT]AC
ApaI 841 GGGCCC GGGCC^C
AspHI 388 GAGCAC G[AT]GC[AT]^C
AvaI 383 CTCGGG C^[CT]CG[GA]G
BalI 668 TGGCCA TGG^CCA
BanI 304 GGCACC G^G[CT][GA]CC
BanII 841 GGGCCC G[GA]GC[CT]^C
BbsI 58 GAAGAC GAAGAC(2/6)
BcgI 305 GCACCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 491 GGATAC GTATCC(6/5)
BfaI 134 CTAG C^TAG
BlpI 15 GCTGAGC GC^T[ACGT]AGC
BpmI 211 CTGGAG CTGGAG(16/14)
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
BpuAI 58 GAAGAC GAAGAC(2/6)
BsgI 820 CTGCAC GTGCAG(16/14)
BsiHKAI 388 GAGCAC G[AT]GC[AT]^C
BsoBI 383 CTCGGG C^[CT]CG[GA]G
Bsp1407I 35 TGTACA T^GTACA
BsrDI 459 CATTGC GCAATG(2/0)
BsrGI 35 TGTACA T^GTACA
BstEII 687 GGTGACC G^GT[ACGT]ACC
BstPI 687 GGTGACC G^GT[ACGT]ACC
BstXI 671 CCAGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 15 GCTGAGC GC^T[ACGT]AGC
DpnII 312 GATC ^GATC
DraII 840 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 603 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 78 CCGTGG C^C[GA][CT]GG
EcoO109I 840 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 687 GGTGACC G^GT[ACGT]ACC
HinfI 863 GATTC G^A[ACGT]TC
MaeI 134 CTAG C^TAG
MaeII 709 ACGT A^CGT
MboI 312 GATC ^GATC
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 171 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 312 GATC ^GATC
SalI 355 GTCGAC G^TCGAC
Sau3AI 312 GATC ^GATC
SpeI 133 ACTAGT A^CTAGT
SphI 798 GCATGC GCATG^C
SspBI 35 TGTACA T^GTACA
StuI 656 AGGCCT AGG^CCT
TfiI 863 GATTC G^A[AT]TC
XcmI 146 CCAGGGTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 143 CCGC CCGC(-3/-1)
25 GCGG
AcsI 274 GAATTT [GA]^AATT[CT]
438 AAATTT
AfaI 36 GTAC GT^AC
793 GTAC
ApoI 274 GAATTT [GA]^AATT[CT]
438 AAATTT
AvaII 140 GGTCC G^G[AT]CC
741 GGACC
BmyI 388 GAGCAC G[AGT]GC[ACT]^C
841 GGGCCC
BseRI 287 GAGGAG GAGGAG(10/8)
652 GAGGAG
BsmAI 769 GAGAC GTCTC(1/5)
849 GAGAC
BsmFI 199 GGGAC GGGAC(10/14)
589 GTCCC
Bsp1286I 388 GAGCAC G[AGT]GC[ACT]^C
841 GGGCCC
BsrI 772 ACTGG ACTGG(1/-1)
260 CCAGT
DdeI 16 CTGAG C^T[ACGT]AG
574 CTAAG
EaeI 75 TGGCCG [CT]^GGCC[GA]
668 TGGCCA
EcoT14I 300 CCTTGG C^C[AT][AT]GG
496 CCAAGG
HincII 247 GTTGAC GT[CT]^[GA]AC
355 GTCGAC
HindII 247 GTTGAC GT[CT]^[GA]AC
355 GTCGAC
HphI 292 GGTGA GGTGA(8/7)
687 GGTGA
MseI 338 TTAA T^TAA
361 TTAA
MwoI 463 GCTGTAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
611 GCAAGATTGGC
NspI 627 GCATGT [GA]CATG^[CT]
798 GCATGC
RsaI 36 GTAC GT^AC
793 GTAC
StyI 300 CCTTGG C^C[AT][AT]GG
496 CCAAGG
Tru9I 338 TTAA T^TAA
361 TTAA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcyI AluI BbvI BsaHI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I EarI EcoRII Fnu4HI
FokI HaeIII HgaI Hin1I ItaI
Ksp632I MaeIII MboII MnlI MvaI
NlaIII NlaIV Sau96I ScrFI SfaNI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I