(Circular) (Six-base) MAPSORT of: pFN21AA0116.seq Check: 7936 from: 1 to: 5578 pFN21AA0116 5578 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2361 2921 1937 Size: 424 560 4594 Fragments arranged by size: 4594 560 424 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 3641 5025 5293 1632 Size: 1004 1005 1384 268 1917 Fragments arranged by size: 1917 1384 1005 1004 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2816 1184 Size: 1632 3946 AlwNI CAG_nnn'CTG Cuts at: 3321 4893 3321 Size: 1572 4006 ApaI G_GGCC'C Cuts at: 2850 2850 Size: 5578 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2279 2443 2887 3113 3265 5374 1493 Size: 786 164 444 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 444 226 164 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2388 2903 1870 Size: 73 445 515 4545 Fragments arranged by size: 4545 515 445 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 2899 4354 618 Size: 325 217 627 522 590 1455 1842 Fragments arranged by size: 1842 1455 627 590 522 325 217 BanII G_rGCy'C Cuts at: 1391 2850 2897 1391 Size: 1459 47 4072 Fragments arranged by size: 4072 1459 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 928 Size: 971 171 4436 Fragments arranged by size: 4436 971 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2299 2333 3769 3803 1903 Size: 34 362 34 1436 34 3678 Fragments arranged by size: 3678 1436 362 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2490 3470 4680 2490 Size: 980 1210 3388 Fragments arranged by size: 3388 1210 980 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2021 2995 2021 Size: 974 4604 Bme1580I G_kGCm'C Cuts at: 1790 2850 3639 4795 1790 Size: 1060 789 1156 2573 Fragments arranged by size: 2573 1156 1060 789 BmgBI CAC'GTC Cuts at: 1318 2715 1318 Size: 1397 4181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3309 4267 442 Size: 416 53 83 399 24 1892 958 1753 Fragments arranged by size: 1892 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2683 2683 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2237 4238 1239 Size: 998 2001 2579 Fragments arranged by size: 2579 2001 998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 4568 4866 3703 Size: 865 298 4415 Fragments arranged by size: 4415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2055 2207 3385 Size: 53 83 186 936 476 46 152 1178 Cuts at: 3385 3767 4402 275 Size: 382 635 1451 Fragments arranged by size: 1451 1178 936 635 476 382 186 152 83 53 46 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 4702 Fragments arranged by size: 4702 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2307 2672 1186 Size: 1121 365 4092 Fragments arranged by size: 4092 1121 365 BseYI C'CCAG_C Cuts at: 2161 2400 4781 5277 2161 Size: 239 2381 496 2462 Fragments arranged by size: 2462 2381 496 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 1163 Size: 573 1074 3931 Fragments arranged by size: 3931 1074 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3789 4817 1540 Size: 275 186 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2397 3639 3724 4795 2397 Size: 1242 85 1071 3180 Fragments arranged by size: 3180 1242 1071 85 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 2850 2897 3639 3724 4795 1391 Size: 399 607 453 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 607 453 399 85 47 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 844 Size: 2089 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3465 1394 Size: 429 1642 3507 Fragments arranged by size: 3507 1642 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2463 4073 4247 65 Size: 1183 1215 1610 174 1396 Fragments arranged by size: 1610 1396 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4222 4409 1700 Size: 110 2412 187 2869 Fragments arranged by size: 2869 2412 187 110 BsrGI T'GTAC_A Cuts at: 96 2040 96 Size: 1944 3634 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5578 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 2692 1184 Size: 1508 4070 BstXI CCAn_nnnn'nTGG Cuts at: 2683 2683 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2908 3275 3660 3677 5129 5215 5227 5573 Size: 936 367 385 17 1452 86 12 346 Cuts at: 5573 1972 Size: 1977 Fragments arranged by size: 1977 1452 936 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2083 513 Size: 552 939 79 4008 Fragments arranged by size: 4008 939 552 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2362 2395 3094 3888 3908 2362 Size: 33 699 794 20 4032 Fragments arranged by size: 4032 794 699 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2122 2157 529 Size: 35 1558 35 3950 Fragments arranged by size: 3950 1558 35 35 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2613 2613 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2673 3025 Size: 54 1425 50 275 18 250 593 352 Cuts at: 3025 3914 5543 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 593 352 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 2520 2655 3508 1353 Size: 1136 31 135 853 3423 Fragments arranged by size: 3423 1136 853 135 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 2137 4165 4539 4685 387 Size: 1064 138 456 92 2028 374 146 1280 Fragments arranged by size: 2028 1280 1064 456 374 146 138 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2636 3641 4238 5025 5293 1239 Size: 393 605 399 1005 597 787 268 1524 Fragments arranged by size: 1524 1005 787 605 597 399 393 268 EcoO109I rG'GnC_Cy Cuts at: 2846 2846 Size: 5578 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 4725 1678 Size: 3047 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2611 2643 4219 Size: 32 135 32 542 32 465 32 1576 Cuts at: 4219 4251 4293 4325 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1576 542 465 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2254 2362 2922 3168 669 Size: 988 281 316 108 560 246 3079 Fragments arranged by size: 3079 988 560 316 281 246 108 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 2845 4692 4876 1305 Size: 852 688 1847 184 2007 Fragments arranged by size: 2007 1847 852 688 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 2913 Fragments arranged by size: 2913 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2180 3536 3895 4054 518 Size: 648 1014 1356 359 159 2042 Fragments arranged by size: 2042 1356 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2994 3671 4819 5064 1606 Size: 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4087 Fragments arranged by size: 4087 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2636 2807 2936 4481 1132 Size: 97 1407 171 129 1545 2229 Fragments arranged by size: 2229 1545 1407 171 129 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PfoI T'CCnGG_A Cuts at: 2058 2058 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2846 2846 Size: 5578 PstI C_TGCA'G Cuts at: 830 2930 830 Size: 2100 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2360 2920 1936 Size: 424 560 4594 Fragments arranged by size: 4594 560 424 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2926 4061 4742 4933 826 Size: 219 1881 1135 681 191 1471 Fragments arranged by size: 1881 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2905 1872 Size: 1033 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 4583 4845 820 Size: 197 926 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 2138 152 Size: 1986 3592 SphI G_CATG'C Cuts at: 1132 2807 2936 1132 Size: 1675 129 3774 Fragments arranged by size: 3774 1675 129 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 2663 1517 Size: 1146 4432 StyI C'CwwG_G Cuts at: 513 1065 2004 2305 2501 3017 513 Size: 552 939 301 196 516 3074 Fragments arranged by size: 3074 939 552 516 301 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 3824 5536 Size: 274 80 33 51 1443 63 1784 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1784 1712 1443 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2158 2158 Size: 5578 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2903 1870 Size: 1033 4545 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI