(Circular) (Six-base) MAPSORT of: pFN21AA0110.seq Check: 7280 from: 1 to: 5527 pFN21AA0110 5527 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2170 2848 2170 Size: 678 4849 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 2597 3590 4974 5242 1632 Size: 719 246 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 719 268 246 AflII C'TTAA_G Cuts at: 820 1017 2285 820 Size: 197 1268 4062 Fragments arranged by size: 4062 1268 197 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4336 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 2048 2545 3270 4842 2048 Size: 497 725 1572 2733 Fragments arranged by size: 2733 1572 725 497 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2343 2852 1870 Size: 73 400 509 4545 Fragments arranged by size: 4545 509 400 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2427 2460 2427 Size: 33 5494 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2170 2633 2797 2848 Size: 325 217 627 224 159 463 164 51 Cuts at: 2848 4303 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 463 325 224 217 164 159 51 BanII G_rGCy'C Cuts at: 1391 2846 1391 Size: 1455 4072 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 5527 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2784 928 Size: 971 885 3671 Fragments arranged by size: 3671 971 885 BbvCI CC'TCA_GC Cuts at: 2035 2408 2035 Size: 373 5154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3718 3752 1903 Size: 34 1781 34 3678 Fragments arranged by size: 3678 1781 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2205 4138 Size: 107 122 71 631 605 337 196 1933 Cuts at: 4138 136 Size: 1525 Fragments arranged by size: 1933 1525 631 605 337 196 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2944 2944 Size: 5527 Bme1580I G_kGCm'C Cuts at: 1790 2636 2709 3588 4744 1790 Size: 846 73 879 1156 2573 Fragments arranged by size: 2573 1156 879 846 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3258 4216 442 Size: 416 53 83 399 24 1841 958 1753 Fragments arranged by size: 1841 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2438 4187 1239 Size: 1178 21 1749 2579 Fragments arranged by size: 2579 1749 1178 21 Bpu10I CC'TnA_GC Cuts at: 2035 2408 2480 2546 2035 Size: 373 72 66 5016 Fragments arranged by size: 5016 373 72 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 3652 4517 4815 2126 Size: 1526 865 298 2838 Fragments arranged by size: 2838 1526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3318 493 Size: 711 2114 2702 Fragments arranged by size: 2702 2114 711 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3334 3716 4351 Size: 53 83 186 936 479 1322 382 635 Cuts at: 4351 275 Size: 1451 Fragments arranged by size: 1451 1322 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 1178 Size: 30 165 30 612 30 4660 Fragments arranged by size: 4660 612 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2610 1186 Size: 1150 274 4103 Fragments arranged by size: 4103 1150 274 BseYI C'CCAG_C Cuts at: 2708 4730 5226 2708 Size: 2022 496 3009 Fragments arranged by size: 3009 2022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4954 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2552 3588 3673 4744 2552 Size: 1036 85 1071 3335 Fragments arranged by size: 3335 1071 1036 85 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 BsmBI CGTCTCn'nnnn_ Cuts at: 2576 2576 Size: 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2552 2636 2709 2846 3588 3673 4744 Size: 399 762 84 73 137 742 85 1071 Cuts at: 4744 1391 Size: 2174 Fragments arranged by size: 2174 1071 762 742 399 137 85 84 73 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2882 844 Size: 2038 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3414 1394 Size: 429 1591 3507 Fragments arranged by size: 3507 1591 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2479 4022 4196 65 Size: 1183 1231 1543 174 1396 Fragments arranged by size: 1543 1396 1231 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4171 4358 1700 Size: 110 2361 187 2869 Fragments arranged by size: 2869 2361 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 3587 4599 3587 Size: 1012 4515 BstAPI GCAn_nnn'nTGC Cuts at: 794 2373 794 Size: 1579 3948 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstXI CCAn_nnnn'nTGG Cuts at: 2590 2590 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522 Size: 885 367 385 17 1452 86 12 346 Cuts at: 5522 1972 Size: 1977 Fragments arranged by size: 1977 1452 885 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2766 513 Size: 552 939 762 3274 Fragments arranged by size: 3274 939 762 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3837 3857 3043 Size: 794 20 4713 Fragments arranged by size: 4713 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2812 2831 2812 Size: 19 5508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492 Size: 54 1425 50 275 18 1144 889 1629 Cuts at: 5492 8 Size: 43 Fragments arranged by size: 1629 1425 1144 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2579 3457 1353 Size: 1226 878 3423 Fragments arranged by size: 3423 1226 878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 4114 4488 4634 387 Size: 1064 138 447 2078 374 146 1280 Fragments arranged by size: 2078 1280 1064 447 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2417 2438 2597 3590 4187 4974 Size: 393 719 66 21 159 993 597 787 Cuts at: 4974 5242 1239 Size: 268 1524 Fragments arranged by size: 1524 993 787 719 597 393 268 159 66 21 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2015 4674 1678 Size: 337 2659 2531 Fragments arranged by size: 2659 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2676 2708 2742 Size: 32 135 32 443 32 629 32 34 Cuts at: 2742 2774 4168 4200 4242 4274 1373 Size: 32 1394 32 42 32 2626 Fragments arranged by size: 2626 1394 629 443 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KasI G'GCGC_C Cuts at: 2011 2011 Size: 5527 KpnI G_GTAC'C Cuts at: 2174 2852 2174 Size: 678 4849 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3705 Fragments arranged by size: 3705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2588 3485 3844 4003 518 Size: 648 1191 231 897 359 159 2042 Fragments arranged by size: 2042 1191 897 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 2943 3620 4768 5013 1606 Size: 1337 677 1148 245 2120 Fragments arranged by size: 2120 1337 1148 677 245 NarI GG'CG_CC Cuts at: 2012 2012 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4036 Fragments arranged by size: 4036 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2885 4430 1132 Size: 97 1656 1545 2229 Fragments arranged by size: 2229 1656 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 2772 1108 Size: 1664 3863 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PstI C_TGCA'G Cuts at: 830 2879 830 Size: 2049 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2527 2875 4010 4691 4882 826 Size: 219 1482 348 1135 681 191 1471 Fragments arranged by size: 1482 1471 1135 681 348 219 191 SfoI GGC'GCC Cuts at: 2013 2013 Size: 5527 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2854 1872 Size: 982 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2285 3631 4532 4794 820 Size: 197 926 162 180 1346 901 262 1553 Fragments arranged by size: 1553 1346 926 901 262 197 180 162 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 2440 152 Size: 2288 3239 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 2147 1517 Size: 630 4897 StyI C'CwwG_G Cuts at: 513 1065 2004 2464 2487 2966 513 Size: 552 939 460 23 479 3074 Fragments arranged by size: 3074 939 552 479 460 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3554 3571 3724 3909 4354 325 Size: 1255 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3773 5485 96 Size: 274 80 33 51 1443 1796 1712 138 Fragments arranged by size: 1796 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5487 5519 5487 Size: 32 5495 Tth111I GACn'n_nGTC Cuts at: 1316 2737 1316 Size: 1421 4106 XbaI T'CTAG_A Cuts at: 2863 2863 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2852 1870 Size: 982 4545 XmnI GAAnn'nnTTC Cuts at: 2305 3656 2305 Size: 1351 4176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI