Restriction map for
pFN21AA0095
(Linear) (Six-base) MAPSORT of: KIAA0095.seq Check: 2456 from: 1 to: 2457
KIAA0095.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 608 2457
Size: 608 1849
AatII G_ACGT'C
Cuts at: 0 1196 2457
Size: 1196 1261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 325 745 1267 1516 2008 2457
Size: 13 312 420 522 249 492 449
Fragments arranged by size:
522 492 449 420 312 249 13
AfeI AGC'GCT
Cuts at: 0 2146 2457
Size: 2146 311
AflIII A'CryG_T
Cuts at: 0 665 695 1691 2457
Size: 665 30 996 766
Fragments arranged by size:
996 766 665 30
AleI CACnn'nnGTG
Cuts at: 0 811 2457
Size: 811 1646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2047 2079 2457
Size: 2047 32 378
Fragments arranged by size:
2047 378 32
ApaI G_GGCC'C
Cuts at: 0 1134 2457
Size: 1134 1323
ApoI r'AATT_y
Cuts at: 0 428 563 828 2047 2205 2457
Size: 428 135 265 1219 158 252
Fragments arranged by size:
1219 428 265 252 158 135
AvaI C'yCGr_G
Cuts at: 0 190 197 2457
Size: 190 7 2260
Fragments arranged by size:
2260 190 7
BanI G'GyrC_C
Cuts at: 0 855 1279 2157 2457
Size: 855 424 878 300
Fragments arranged by size:
878 855 424 300
BanII G_rGCy'C
Cuts at: 0 26 1023 1134 1942 2028 2457
Size: 26 997 111 808 86 429
Fragments arranged by size:
997 808 429 111 86 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1343 2457
Size: 464 879 1114
Fragments arranged by size:
1114 879 464
BbvCI CC'TCA_GC
Cuts at: 0 374 1537 2457
Size: 374 1163 920
Fragments arranged by size:
1163 920 374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2415 2449 2457
Size: 2415 34 8
Fragments arranged by size:
2415 34 8
BclI T'GATC_A
Cuts at: 0 2084 2457
Size: 2084 373
BlpI GC'TnA_GC
Cuts at: 0 1529 1936 2457
Size: 1529 407 521
Fragments arranged by size:
1529 521 407
Bme1580I G_kGCm'C
Cuts at: 0 1134 1965 2162 2457
Size: 1134 831 197 295
Fragments arranged by size:
1134 831 295 197
BmgBI CAC'GTC
Cuts at: 0 152 696 2457
Size: 152 544 1761
Fragments arranged by size:
1761 544 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 84 2457
Size: 84 2373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1627 1805 1936 2457
Size: 1627 178 131 521
Fragments arranged by size:
1627 521 178 131
Bpu10I CC'TnA_GC
Cuts at: 0 374 1537 2457
Size: 374 1163 920
Fragments arranged by size:
1163 920 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 461 805 2012 2272 2457
Size: 289 172 344 1207 260 185
Fragments arranged by size:
1207 344 289 260 185 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 980 2457
Size: 200 780 1477
Fragments arranged by size:
1477 780 200
BsaHI Gr'CG_yC
Cuts at: 0 178 461 1193 2457
Size: 178 283 732 1264
Fragments arranged by size:
1264 732 283 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 2457
Size: 382 30 2045
Fragments arranged by size:
2045 382 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 391 1525 1582 2457
Size: 178 213 1134 57 875
Fragments arranged by size:
1134 875 213 178 57
BseYI C'CCAG_C
Cuts at: 0 224 842 846 1022 1032 1092 2321 2457
Size: 224 618 4 176 10 60 1229 136
Fragments arranged by size:
1229 618 224 176 136 60 10 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 418 2455 2457
Size: 266 152 2037 2
Fragments arranged by size:
2037 266 152 2
BsiHKAI G_wGCw'C
Cuts at: 0 26 1530 2457
Size: 26 1504 927
Fragments arranged by size:
1504 927 26
BsmI GAATG_Cn'
Cuts at: 0 1905 2457
Size: 1905 552
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 2457
Size: 154 2303
Bsp1286I G_dGCh'C
Cuts at: 0 26 1023 1134 1530 1942 1965 2028 2162
Size: 26 997 111 396 412 23 63 134
Cuts at: 2162 2457
Size: 295
Fragments arranged by size:
997 412 396 295 134 111 63 26
23
BspHI T'CATG_A
Cuts at: 0 2450 2457
Size: 2450 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 608 998 2457
Size: 608 390 1459
Fragments arranged by size:
1459 608 390
BsrBI CCG'CTC
Cuts at: 0 1449 2457
Size: 1449 1008
BsrDI GCAATG_nn'
Cuts at: 0 2006 2457
Size: 2006 451
BsrFI r'CCGG_y
Cuts at: 0 708 2457
Size: 708 1749
BsrGI T'GTAC_A
Cuts at: 0 1601 2457
Size: 1601 856
BssSI C'ACGA_G
Cuts at: 0 1543 2457
Size: 1543 914
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 718 1426 2457
Size: 441 277 708 1031
Fragments arranged by size:
1031 708 441 277
BstBI TT'CG_AA
Cuts at: 0 879 2457
Size: 879 1578
BstEII G'GTnAC_C
Cuts at: 0 1552 2457
Size: 1552 905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1549 2457
Size: 1549 908
BstYI r'GATC_y
Cuts at: 0 104 1149 2457
Size: 104 1045 1308
Fragments arranged by size:
1308 1045 104
Bsu36I CC'TnA_GG
Cuts at: 0 1135 1660 2457
Size: 1135 525 797
Fragments arranged by size:
1135 797 525
BtgI C'CryG_G
Cuts at: 0 674 1622 2457
Size: 674 948 835
Fragments arranged by size:
948 835 674
BtsI GCAGTG_nn'
Cuts at: 0 439 2457
Size: 439 2018
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 240 275 2457
Size: 240 35 2182
Fragments arranged by size:
2182 240 35
DraI TTT'AAA
Cuts at: 0 1044 1508 2457
Size: 1044 464 949
Fragments arranged by size:
1044 949 464
DrdI GACnn_nn'nnGTC
Cuts at: 0 518 2457
Size: 518 1939
EarI CTCTTCn'nnn_
Cuts at: 0 338 349 1862 2177 2457
Size: 338 11 1513 315 280
Fragments arranged by size:
1513 338 315 280 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1234 2457
Size: 1234 1223
EcoICRI GAG'CTC
Cuts at: 0 24 2457
Size: 24 2433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 325 745 1267 1516 1627 1805 1936
Size: 13 312 420 522 249 111 178 131
Cuts at: 1936 2008 2457
Size: 72 449
Fragments arranged by size:
522 449 420 312 249 178 131 111
72 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2167 2457
Size: 2167 290
EcoO109I rG'GnC_Cy
Cuts at: 0 359 511 1130 1131 1638 2318 2445 2457
Size: 359 152 619 1 507 680 127 12
Fragments arranged by size:
680 619 507 359 152 127 12 1
EcoRI G'AATT_C
Cuts at: 0 428 2457
Size: 428 2029
EcoRV GAT'ATC
Cuts at: 0 2136 2457
Size: 2136 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 481 2263 2457
Size: 378 103 1782 194
Fragments arranged by size:
1782 378 194 103
FspI TGC'GCA
Cuts at: 0 751 2457
Size: 751 1706
FspAI rTGC'GCAy
Cuts at: 0 751 2457
Size: 751 1706
HaeII r_GCGC'y
Cuts at: 0 126 961 2148 2457
Size: 126 835 1187 309
Fragments arranged by size:
1187 835 309 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1178 1210 2329 2361 2457
Size: 1178 32 1119 32 96
Fragments arranged by size:
1178 1119 96 32 32
HincII GTy'rAC
Cuts at: 0 704 2457
Size: 704 1753
MfeI C'AATT_G
Cuts at: 0 871 2457
Size: 871 1586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1313 1997 2457
Size: 686 627 684 460
Fragments arranged by size:
686 684 627 460
MslI CAynn'nnrTG
Cuts at: 0 544 811 2457
Size: 544 267 1646
Fragments arranged by size:
1646 544 267
MspA1I CmG'CkG
Cuts at: 0 846 1032 2457
Size: 846 186 1425
Fragments arranged by size:
1425 846 186
NcoI C'CATG_G
Cuts at: 0 674 2457
Size: 674 1783
NspI r_CATG'y
Cuts at: 0 669 978 1695 2457
Size: 669 309 717 762
Fragments arranged by size:
762 717 669 309
PciI A'CATG_T
Cuts at: 0 665 1691 2457
Size: 665 1026 766
Fragments arranged by size:
1026 766 665
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 2457
Size: 231 2226
PfoI T'CCnGG_A
Cuts at: 0 349 1055 2457
Size: 349 706 1402
Fragments arranged by size:
1402 706 349
PpuMI rG'GwC_Cy
Cuts at: 0 359 511 2445 2457
Size: 359 152 1934 12
Fragments arranged by size:
1934 359 152 12
PsiI TTA'TAA
Cuts at: 0 797 2457
Size: 797 1660
PspOMI G'GGCC_C
Cuts at: 0 1130 2457
Size: 1130 1327
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 80 112 2457
Size: 80 32 2345
Fragments arranged by size:
2345 80 32
PstI C_TGCA'G
Cuts at: 0 605 2457
Size: 605 1852
PvuII CAG'CTG
Cuts at: 0 846 1032 2457
Size: 846 186 1425
Fragments arranged by size:
1425 846 186
SacI G_AGCT'C
Cuts at: 0 26 2457
Size: 26 2431
SanDI GG'GwC_CC
Cuts at: 0 511 2457
Size: 511 1946
ScaI AGT'ACT
Cuts at: 0 1648 2457
Size: 1648 809
SexAI A'CCwGG_T
Cuts at: 0 611 1048 2457
Size: 611 437 1409
Fragments arranged by size:
1409 611 437
SfcI C'TryA_G
Cuts at: 0 601 922 2457
Size: 601 321 1535
Fragments arranged by size:
1535 601 321
SmlI C'TyrA_G
Cuts at: 0 268 476 784 2027 2287 2457
Size: 268 208 308 1243 260 170
Fragments arranged by size:
1243 308 268 260 208 170
SpeI A'CTAG_T
Cuts at: 0 1804 2457
Size: 1804 653
SphI G_CATG'C
Cuts at: 0 978 2457
Size: 978 1479
StuI AGG'CCT
Cuts at: 0 772 2457
Size: 772 1685
StyI C'CwwG_G
Cuts at: 0 674 773 1630 2457
Size: 674 99 857 827
Fragments arranged by size:
857 827 674 99
TatI w'GTAC_w
Cuts at: 0 1061 1169 1601 1646 2457
Size: 1061 108 432 45 811
Fragments arranged by size:
1061 811 432 108 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1225 1257 2457
Size: 1225 32 1200
Fragments arranged by size:
1225 1200 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2427 2457
Size: 2427 30
XmnI GAAnn'nnTTC
Cuts at: 0 2243 2457
Size: 2243 214
ZraI GAC'GTC
Cuts at: 0 1194 2457
Size: 1194 1263
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AloI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraI DrdI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII MfeI MmeI MslI MspA1I NcoI NspI PciI
PflMI PfoI PpuMI PsiI PspOMI PsrI PstI PvuII
SacI SanDI ScaI SexAI SfcI SmlI SpeI SphI
StuI StyI TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BfrBI BglI BglII BmrI BmtI BsaAI BsaBI
BsaWI BsiEI BsiWI BspEI BssHII BstZ17I BtgZI ClaI
DraIII EaeI EagI FseI HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PspXI PvuI RsrII SacII SalI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI