(Circular) (Six-base) MAPSORT of: pFN21AA0092.seq Check: 9415 from: 1 to: 6127 pFN21AA0092 6127 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3344 4190 5574 5842 1632 Size: 1712 846 1384 268 1917 Fragments arranged by size: 1917 1712 1384 846 268 AflII C'TTAA_G Cuts at: 820 1017 2222 820 Size: 197 1205 4725 Fragments arranged by size: 4725 1205 197 AflIII A'CryG_T Cuts at: 1203 2545 5026 6003 1203 Size: 1342 2481 977 1327 Fragments arranged by size: 2481 1342 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2634 1184 Size: 1450 4677 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2073 2105 2073 Size: 32 6095 AlwNI CAG_nnn'CTG Cuts at: 3870 5442 3870 Size: 1572 4555 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2353 3035 3391 3436 3662 3814 5923 1493 Size: 860 682 356 45 226 152 2109 1697 Fragments arranged by size: 2109 1697 860 682 356 226 152 45 AseI AT'TA_AT Cuts at: 160 1032 2994 4681 160 Size: 872 1962 1687 1606 Fragments arranged by size: 1962 1687 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3446 1391 Size: 2055 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 2536 3055 1548 Size: 988 519 4620 Fragments arranged by size: 4620 988 519 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4738 136 Size: 107 122 71 631 605 337 2729 1525 Fragments arranged by size: 2729 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 2061 3544 2061 Size: 1483 4644 Bme1580I G_kGCm'C Cuts at: 1790 4188 5344 1790 Size: 2398 1156 2573 Fragments arranged by size: 2573 2398 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3221 3357 4787 1239 Size: 1982 136 1430 2579 Fragments arranged by size: 2579 1982 1430 136 Bpu10I CC'TnA_GC Cuts at: 2691 2691 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 4252 5117 5415 2279 Size: 1973 865 298 2991 Fragments arranged by size: 2991 1973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3325 4790 882 Size: 617 1826 1465 2219 Fragments arranged by size: 2219 1826 1465 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3934 4316 4951 Size: 53 83 186 936 482 1919 382 635 Cuts at: 4951 275 Size: 1451 Fragments arranged by size: 1919 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5902 Fragments arranged by size: 5902 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2971 3014 1186 Size: 1785 43 4299 Fragments arranged by size: 4299 1785 43 BseYI C'CCAG_C Cuts at: 3195 5330 5826 3195 Size: 2135 496 3496 Fragments arranged by size: 3496 2135 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4188 4273 5344 4188 Size: 85 1071 4971 Fragments arranged by size: 4971 1071 85 BsmI GAATG_Cn' Cuts at: 2095 3638 3731 2095 Size: 1543 93 4491 Fragments arranged by size: 4491 1543 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3446 4188 4273 5344 1391 Size: 399 1656 742 85 1071 2174 Fragments arranged by size: 2174 1656 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2899 4622 4796 65 Size: 1183 1651 1723 174 1396 Fragments arranged by size: 1723 1651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 2940 4187 5199 2940 Size: 1247 1012 3868 Fragments arranged by size: 3868 1247 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 3181 4931 3181 Size: 1750 4377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2572 3457 3824 4209 4226 5678 5764 5776 Size: 600 885 367 385 17 1452 86 12 Cuts at: 5776 6122 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 885 600 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2832 513 Size: 552 939 828 3808 Fragments arranged by size: 3808 939 828 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DraIII CAC_nnn'GTG Cuts at: 2945 2945 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092 Size: 54 1425 50 275 18 1744 889 1629 Cuts at: 6092 8 Size: 43 Fragments arranged by size: 1744 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3221 3344 3357 4190 4787 5574 5842 Size: 393 1589 123 13 833 597 787 268 Cuts at: 5842 1239 Size: 1524 Fragments arranged by size: 1589 1524 833 787 597 393 268 123 13 EcoO109I rG'GnC_Cy Cuts at: 2614 2614 Size: 6127 EcoRI G'AATT_C Cuts at: 2353 3391 3436 3814 2353 Size: 1038 45 378 4666 Fragments arranged by size: 4666 1038 378 45 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2990 3022 3040 Size: 32 135 32 902 32 484 32 18 Cuts at: 3040 3072 3340 3372 4768 4800 4842 4874 1373 Size: 32 268 32 1396 32 42 32 2626 Fragments arranged by size: 2626 1396 902 484 268 135 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 2883 748 Size: 2135 3992 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 2307 2441 3726 2307 Size: 134 1285 4708 Fragments arranged by size: 4708 1285 134 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 3009 3096 5241 5425 1305 Size: 1317 387 87 2145 184 2007 Fragments arranged by size: 2145 2007 1317 387 184 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4085 4444 4603 518 Size: 648 2919 359 159 2042 Fragments arranged by size: 2919 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2699 3199 3543 4220 5368 5613 1606 Size: 1093 500 344 677 1148 245 2120 Fragments arranged by size: 2120 1148 1093 677 500 344 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4636 Fragments arranged by size: 4636 939 552 NdeI CA'TA_TG Cuts at: 387 2110 387 Size: 1723 4404 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2455 2474 2549 2686 3379 3485 5030 Size: 97 1226 19 75 137 693 106 1545 Cuts at: 5030 1132 Size: 2229 Fragments arranged by size: 2229 1545 1226 693 137 106 97 75 19 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 2545 5026 2545 Size: 2481 3646 PflMI CCAn_nnn'nTGG Cuts at: 1108 2833 1108 Size: 1725 4402 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2492 1613 Size: 879 5248 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2829 2861 2829 Size: 32 6095 PstI C_TGCA'G Cuts at: 830 2413 3479 830 Size: 1583 1066 3478 Fragments arranged by size: 3478 1583 1066 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2699 3199 2699 Size: 500 5627 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 2325 4375 6087 2325 Size: 2050 1712 2365 Fragments arranged by size: 2365 2050 1712 SfcI C'TryA_G Cuts at: 826 1045 2409 3475 4610 5291 5482 826 Size: 219 1364 1066 1135 681 191 1471 Fragments arranged by size: 1471 1364 1135 1066 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2258 4231 5132 5394 820 Size: 197 926 279 36 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 279 262 197 36 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 2474 2686 3485 1132 Size: 1342 212 799 3774 Fragments arranged by size: 3774 1342 799 212 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 3566 513 Size: 552 939 1562 3074 Fragments arranged by size: 3074 1562 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2323 4373 6085 Size: 274 80 33 51 1443 346 2050 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 2050 1712 1443 346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 2297 2716 3463 2297 Size: 419 747 4961 Fragments arranged by size: 4961 747 419 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI