(Circular) (Six-base) MAPSORT of: pFN21AA0089.seq Check: 7755 from: 1 to: 5758 pFN21AA0089 5758 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 3101 1937 Size: 816 348 4594 Fragments arranged by size: 4594 816 348 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 2943 3020 3821 5205 5473 1632 Size: 449 862 77 801 1384 268 1917 Fragments arranged by size: 1917 1384 862 801 449 268 77 AfeI AGC'GCT Cuts at: 2349 2349 Size: 5758 AflII C'TTAA_G Cuts at: 820 1017 2618 820 Size: 197 1601 3960 Fragments arranged by size: 3960 1601 197 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2934 1184 Size: 1750 4008 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2038 2070 2038 Size: 32 5726 AlwNI CAG_nnn'CTG Cuts at: 2313 2597 3501 5073 2313 Size: 284 904 1572 2998 Fragments arranged by size: 2998 1572 904 284 ApaI G_GGCC'C Cuts at: 2387 2387 Size: 5758 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2102 2563 3067 3293 3445 5554 1493 Size: 609 461 504 226 152 2109 1697 Fragments arranged by size: 2109 1697 609 504 461 226 152 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2387 3083 1870 Size: 73 98 346 696 4545 Fragments arranged by size: 4545 696 346 98 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 3079 4534 618 Size: 325 217 627 227 1065 1455 1842 Fragments arranged by size: 1842 1455 1065 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2042 2387 3077 1391 Size: 651 345 690 4072 Fragments arranged by size: 4072 690 651 345 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 5758 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 3026 928 Size: 971 962 165 3660 Fragments arranged by size: 3660 971 962 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 2793 1548 Size: 1245 4513 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2991 5753 2991 Size: 2762 2996 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2340 2387 3819 4975 1790 Size: 550 47 1432 1156 2573 Fragments arranged by size: 2573 1432 1156 550 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 2337 2859 Size: 416 53 83 399 24 642 278 522 Cuts at: 2859 3489 4447 442 Size: 630 958 1753 Fragments arranged by size: 1753 958 642 630 522 416 399 278 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4418 1239 Size: 3179 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 4421 882 Size: 617 993 1929 2219 Fragments arranged by size: 2219 1929 993 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3565 3947 4582 Size: 53 83 186 936 482 1550 382 635 Cuts at: 4582 275 Size: 1451 Fragments arranged by size: 1550 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5758 BseYI C'CCAG_C Cuts at: 2350 2642 4961 5457 2350 Size: 292 2319 496 2651 Fragments arranged by size: 2651 2319 496 292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 1163 Size: 573 543 4642 Fragments arranged by size: 4642 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2688 3208 3969 4997 1540 Size: 275 186 687 520 761 1028 2301 Fragments arranged by size: 2301 1028 761 687 520 275 186 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2340 2387 3077 3819 3904 4975 Size: 399 252 298 47 690 742 85 1071 Cuts at: 4975 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 690 399 298 252 85 47 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 3113 844 Size: 1436 833 3489 Fragments arranged by size: 3489 1436 833 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2463 4253 4427 65 Size: 1183 1215 1790 174 1396 Fragments arranged by size: 1790 1396 1215 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5758 BstBI TT'CG_AA Cuts at: 2569 4562 2569 Size: 1993 3765 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2730 2991 3088 3455 3840 3857 5309 5395 Size: 758 261 97 367 385 17 1452 86 Cuts at: 5395 5407 5753 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 758 385 367 346 261 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2988 3274 4068 4088 2988 Size: 286 794 20 4658 Fragments arranged by size: 4658 794 286 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 2784 2829 Size: 54 1425 50 275 18 855 99 45 Cuts at: 2829 3205 4094 5723 8 Size: 376 889 1629 43 Fragments arranged by size: 1629 1425 889 855 376 275 99 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2685 3205 1537 Size: 275 873 520 4090 Fragments arranged by size: 4090 873 520 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2859 3688 1353 Size: 1190 316 829 3423 Fragments arranged by size: 3423 1190 829 316 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2684 2830 4345 4719 4865 387 Size: 1064 138 1095 146 1515 374 146 1280 Fragments arranged by size: 1515 1280 1095 1064 374 146 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2943 3020 3821 4418 5205 5473 Size: 393 449 862 77 801 597 787 268 Cuts at: 5473 1239 Size: 1524 Fragments arranged by size: 1524 862 801 787 597 449 393 268 77 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 5758 EcoO109I rG'GnC_Cy Cuts at: 2383 2384 2383 Size: 1 5757 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 2018 2351 4905 1678 Size: 340 333 2554 2531 Fragments arranged by size: 2554 2531 340 333 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3011 3043 4399 4431 4473 Size: 32 135 32 1439 32 1356 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 1439 1356 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KasI G'GCGC_C Cuts at: 2014 2014 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2850 4872 5056 1305 Size: 1545 2022 184 2007 Fragments arranged by size: 2022 2007 1545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2444 2711 3716 4075 4234 518 Size: 648 1021 257 267 1005 359 159 2042 Fragments arranged by size: 2042 1021 1005 648 359 267 257 159 MspA1I CmG'CkG Cuts at: 1606 2012 2060 2664 3174 3851 4999 5244 1606 Size: 406 48 604 510 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 604 510 406 245 48 NarI GG'CG_CC Cuts at: 2015 2015 Size: 5758 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PfoI T'CCnGG_A Cuts at: 2203 2203 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2383 2383 Size: 5758 PstI C_TGCA'G Cuts at: 830 2485 2556 2983 3110 830 Size: 1655 71 427 127 3478 Fragments arranged by size: 3478 1655 427 127 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2060 2060 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2481 2552 2754 2979 3106 4241 4922 Size: 219 1436 71 202 225 127 1135 681 Cuts at: 4922 5113 826 Size: 191 1471 Fragments arranged by size: 1471 1436 1135 681 225 219 202 191 127 71 SfoI GGC'GCC Cuts at: 2016 2016 Size: 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3862 4763 5025 820 Size: 197 926 675 1244 901 262 1553 Fragments arranged by size: 1553 1244 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 2837 1517 Size: 1320 4438 StyI C'CwwG_G Cuts at: 513 1065 2004 2902 3197 513 Size: 552 939 898 295 3074 Fragments arranged by size: 3074 939 898 552 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4004 5716 96 Size: 274 80 33 51 1443 2027 1712 138 Fragments arranged by size: 2027 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2866 2347 Size: 519 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI