Restriction map for
pFN21AB9440
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI FseI HgaI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 1710 CGCG CG^CG
AcyI 92 GGCGCC G[GA]^CG[CT]C
AflII 1662 CTTAAG C^TTAAG
AlwNI 1802 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 14 TGCGCA TGC^GCA
BanII 872 GGGCTC G[GA]GC[CT]^C
BbeI 92 GGCGCC GGCGC^C
BbsI 1217 GTCTTC GAAGAC(2/6)
BclI 1317 TGATCA T^GATCA
BfaI 1295 CTAG C^TAG
BfrI 1662 CTTAAG C^TTAAG
BglI 528 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1285 CTCCAG CTGGAG(16/14)
BpuAI 1217 GTCTTC GAAGAC(2/6)
BsaHI 92 GGCGCC G[GA]^CG[CT]C
BsaI 1048 GAGACC GGTCTC(1/5)
BsaWI 1058 ACCGGA [AT]^CCGG[AT]
BseRI 1627 CTCCTC GAGGAG(10/8)
BsgI 1469 GTGCAG GTGCAG(16/14)
BsmAI 1048 GAGAC GTCTC(1/5)
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BstUI 1710 CGCG CG^CG
BstYI 300 GGATCT [GA]^GATC[CT]
DraI 262 TTTAAA TTT^AAA
DraIII 1594 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 649 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1426 GATATC GAT^ATC
EcoT22I 1823 ATGCAT ATGCA^T
FbaI 1317 TGATCA T^GATCA
FspI 14 TGCGCA TGC^GCA
Hin1I 92 GGCGCC G[GA]^CG[CT]C
HincII 1561 GTTGAC GT[CT]^[GA]AC
HindII 1561 GTTGAC GT[CT]^[GA]AC
KasI 92 GGCGCC G^GCGCC
MaeI 1295 CTAG C^TAG
MflI 300 GGATCT [GA]^GATC[CT]
MvnI 1710 CGCG CG^CG
NarI 92 GGCGCC GG^CGCC
NsiI 1823 ATGCAT ATGCA^T
PstI 1966 CTGCAG CTGCA^G
SfcI 1966 CTGCAG C^T[GA][CT]AG
SfoI 92 GGCGCC GGC^GCC
SmlI 1662 CTTAAG C^T[CT][GA]AG
SspI 1225 AATATT AAT^ATT
XhoII 300 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 619 GTAC GT^AC
1253 GTAC
AlwI 117 GATCC GGATC(4/5)
300 GGATC
Aor51HI 362 AGCGCT AGC^GCT
910 AGCGCT
AvaI 79 CCCGAG C^[CT]CG[GA]G
869 CTCGGG
BalI 1004 TGGCCA TGG^CCA
1411 TGGCCA
BanI 92 GGCGCC G^G[CT][GA]CC
920 GGCACC
BsaAI 462 CACGTA [CT]AC^GT[GA]
793 TACGTG
BsoBI 79 CCCGAG C^[CT]CG[GA]G
869 CTCGGG
BspHI 1277 TCATGA T^CATGA
1736 TCATGA
DsaI 830 CCATGG C^C[GA][CT]GG
1418 CCATGG
Eco47III 362 AGCGCT AGC^GCT
910 AGCGCT
EcoT14I 830 CCATGG C^C[AT][AT]GG
1418 CCATGG
MaeII 463 ACGT A^CGT
794 ACGT
MluNI 1004 TGGCCA TGG^CCA
1411 TGGCCA
MscI 1004 TGGCCA TGG^CCA
1411 TGGCCA
MspA1I 802 CCGCTG C[AC]G^C[GT]G
1121 CCGCTG
NcoI 830 CCATGG C^CATGG
1418 CCATGG
RcaI 1277 TCATGA T^CATGA
1736 TCATGA
RsaI 619 GTAC GT^AC
1253 GTAC
StyI 830 CCATGG C^C[AT][AT]GG
1418 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AluI Alw44I ApaLI
ApoI Asp700I AspHI AvaII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PpuMI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmnI