Restriction map for
pFN21AE2768
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AhdI Alw44I AlwI AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstNI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoRII EcoRV
EcoT14I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI MvaI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 431 CCGC CCGC(-3/-1)
AcsI 51 GAATTC [GA]^AATT[CT]
AgeI 410 ACCGGT A^CCGGT
Aor51HI 621 AGCGCT AGC^GCT
ApaI 634 GGGCCC GGGCC^C
ApoI 51 GAATTC [GA]^AATT[CT]
BbsI 70 GTCTTC GAAGAC(2/6)
BbvCI 445 GCTGAGG CCTCAGC(-5/-2)
BcnI 120 CCCGG CC^[GC]GG
BfaI 619 CTAG C^TAG
BpmI 393 CTCCAG CTGGAG(16/14)
BpuAI 70 GTCTTC GAAGAC(2/6)
BsaWI 410 ACCGGT [AT]^CCGG[AT]
BseRI 313 GAGGAG GAGGAG(10/8)
BsgI 595 CTGCAC GTGCAG(16/14)
BspMI 88 ACCTGC ACCTGC(4/8)
BsrFI 410 ACCGGT [GA]^CCGG[CT]
BssKI 120 CCCGG ^CC[ACGT]GG
BstEII 123 GGTCACC G^GT[ACGT]ACC
BstF5I 183 CATCC GGATG(2/0)
BstPI 123 GGTCACC G^GT[ACGT]ACC
CfoI 622 GCGC GCG^C
Cfr10I 410 ACCGGT [GA]^CCGG[CT]
Eco47III 621 AGCGCT AGC^GCT
EcoO65I 123 GGTCACC G^GT[ACGT]ACC
EcoRI 51 GAATTC G^AATTC
EcoT22I 210 ATGCAT ATGCA^T
FauI 430 CCCGC CCCGC(4/6)
FokI 183 CATCC GGATG(9/13)
HaeII 621 AGCGCT [GA]GCGC^[CT]
HhaI 622 GCGC GCG^C
HinP1I 622 GCGC G^CGC
HindIII 497 AAGCTT A^AGCTT
MaeI 619 CTAG C^TAG
MaeIII 124 GTCAC ^GT[ACGT]AC
MseI 469 TTAA T^TAA
MslI 96 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 431 CCGCTG C[AC]G^C[GT]G
NciI 120 CCCGG CC^[GC]GG
NsiI 210 ATGCAT ATGCA^T
NspI 599 ACATGC [GA]CATG^[CT]
PflMI 287 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 410 ACCGGT A^CCGGT
PleI 583 GAGTC GAGTC(4/5)
SacI 306 GAGCTC GAGCT^C
ScrFI 120 CCCGG CC^[ACGT]GG
Tru9I 469 TTAA T^TAA
Tsp45I 124 GTCAC ^GT[GC]AC
Van91I 287 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 105 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAGATGTTC
AspI 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
422 GACACTGTC
BanII 306 GAGCTC G[GA]GC[CT]^C
634 GGGCCC
BsmFI 653 GGGAC GGGAC(10/14)
428 GTCCC
BsmI 208 GAATGC GAATGC(1/-1)
329 GCATTC
Cac8I 152 GCAGGC GC[ACGT]^[ACGT]GC
374 GCCTGC
DraII 154 AGGCCCT [GA]G^G[ACGT]CC[CT]
633 AGGGCCC
EcoO109I 154 AGGCCCT [GA]G^G[ACGT]CC[CT]
633 AGGGCCC
HaeIII 155 GGCC GG^CC
635 GGCC
HapII 121 CCGG C^CGG
411 CCGG
HpaII 121 CCGG C^CGG
411 CCGG
HphI 86 TCACC GGTGA(8/7)
125 TCACC
MspI 121 CCGG C^CGG
411 CCGG
PflFI 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
422 GACACTGTC
TaqI 6 TCGA T^CGA
252 TCGA
Tth111I 118 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
422 GACACTGTC
TthHB8I 6 TCGA T^CGA
252 TCGA
XmnI 105 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwNI AspHI BbvI BmyI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI Cfr13I DdeI Fnu4HI HinfI
ItaI MboII MnlI MwoI NlaIII
NlaIV Sau96I TfiI TseI Tsp509I
TspRI