Restriction map for
pFN21AE3897
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BseAI BseRI BsgI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 183 GTAC GT^AC
AflIII 311 ACATGT A^C[GA][CT]GT
AluI 489 AGCT AG^CT
AseI 369 ATTAAT AT^TAAT
AsnI 369 ATTAAT AT^TAAT
AspHI 31 GTGCTC G[AT]GC[AT]^C
AvaII 471 GGACC G^G[AT]CC
BbsI 460 GAAGAC GAAGAC(2/6)
BglII 11 AGATCT A^GATCT
BpuAI 460 GAAGAC GAAGAC(2/6)
BsaBI 217 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 67 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 54 TCCGGT [AT]^CCGG[AT]
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
BsmAI 292 GAGAC GTCTC(1/5)
BspLU11I 311 ACATGT A^CATGT
BsrBI 44 CCGCTC CCGCTC(-3/-3)
BstF5I 465 CATCC GGATG(2/0)
BstYI 11 AGATCT [GA]^GATC[CT]
Cfr13I 471 GGACC G^G[ACGT]CC
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
FauI 121 CCCGC CCCGC(4/6)
FokI 465 CATCC GGATG(9/13)
HinfI 521 GAATC G^A[ACGT]TC
MamI 217 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 11 AGATCT [GA]^GATC[CT]
MseI 370 TTAA T^TAA
MwoI 51 GCCTCCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 471 GGACCC GG[ACGT]^[ACGT]CC
NspI 311 ACATGT [GA]CATG^[CT]
PshBI 369 ATTAAT AT^TAAT
RsaI 183 GTAC GT^AC
Sau96I 471 GGACC G^G[ACGT]CC
SfcI 287 CTGTAG C^T[GA][CT]AG
StuI 164 AGGCCT AGG^CCT
TfiI 521 GAATC G^A[AT]TC
Tru9I 370 TTAA T^TAA
Tsp509I 368 AATT ^AATT
TspRI 285 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 186 CCAAGTTTTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 11 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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BmyI 31 GTGCTC G[AGT]GC[ACT]^C
560 GGGCAC
BpmI 233 CTCCAG CTGGAG(16/14)
172 CTGGAG
Bsp1286I 31 GTGCTC G[AGT]GC[ACT]^C
560 GGGCAC
BsrFI 76 GCCGGC [GA]^CCGG[CT]
124 GCCGGC
BsrI 430 ACTGG ACTGG(1/-1)
456 ACTGG
Cac8I 76 GCCGGC GC[ACGT]^[ACGT]GC
124 GCCGGC
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
124 GCCGGC
DpnII 12 GATC ^GATC
177 GATC
MboI 12 GATC ^GATC
177 GATC
NaeI 76 GCCGGC GCC^GGC
124 GCCGGC
NdeII 12 GATC ^GATC
177 GATC
NgoMIV 76 GCCGGC G^CCGGC
124 GCCGGC
NlaIII 153 CATG CATG^
312 CATG
Sau3AI 12 GATC ^GATC
177 GATC
SfaNI 513 GCATC GCATC(5/9)
534 GCATC
TaqI 95 TCGA T^CGA
179 TCGA
TthHB8I 95 TCGA T^CGA
179 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI BbvI BssKI BstNI DdeI
EaeI EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MboII MnlI
MspI MvaI ScrFI TseI