Restriction map for
pFN21AE3098
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AlwI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BfrI BlnI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1285 GTCTAC GT^[AC][GT]AC
AccII 476 CGCG CG^CG
AcsI 820 AAATTC [GA]^AATT[CT]
AflIII 722 ACACGT A^C[GA][CT]GT
Alw44I 745 GTGCAC G^TGCAC
AlwNI 620 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 745 GTGCAC G^TGCAC
ApoI 820 AAATTC [GA]^AATT[CT]
Asp700I 423 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 140 TGGCCA TGG^CCA
BbvCI 617 CCTCAGC CCTCAGC(-5/-2)
BciVI 1082 GTATCC GTATCC(6/5)
BclI 1112 TGATCA T^GATCA
BfaI 289 CTAG C^TAG
BglI 636 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 809 AGATCT A^GATCT
BlpI 585 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 585 GCTCAGC GC^T[ACGT]AGC
BsaAI 723 CACGTA [CT]AC^GT[GA]
BseRI 663 CTCCTC GAGGAG(10/8)
BsgI 706 GTGCAG GTGCAG(16/14)
BsrFI 161 GCCGGC [GA]^CCGG[CT]
BssSI 1200 CACGAG CACGAG(-5/-1)
BstBI 1246 TTCGAA TT^CGAA
BstUI 476 CGCG CG^CG
BstXI 332 CCATGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 809 AGATCT [GA]^GATC[CT]
CelII 585 GCTCAGC GC^T[ACGT]AGC
Cfr10I 161 GCCGGC [GA]^CCGG[CT]
FbaI 1112 TGATCA T^GATCA
HaeII 1067 GGCGCT [GA]GCGC^[CT]
HphI 1181 TCACC GGTGA(8/7)
MaeI 289 CTAG C^TAG
MaeII 724 ACGT A^CGT
MaeIII 1198 GTCAC ^GT[ACGT]AC
MflI 809 AGATCT [GA]^GATC[CT]
MluNI 140 TGGCCA TGG^CCA
MscI 140 TGGCCA TGG^CCA
MseI 230 TTAA T^TAA
MvnI 476 CGCG CG^CG
NaeI 161 GCCGGC GCC^GGC
NgoMIV 161 GCCGGC G^CCGGC
NspV 1246 TTCGAA TT^CGAA
PflFI 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 8 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 850 GAGCTC GAGCT^C
SbfI 1188 CCTGCAGG CCTGCA^GG
SfuI 1246 TTCGAA TT^CGAA
Sse8387I 1188 CCTGCAGG CCTGCA^GG
Tru9I 230 TTAA T^TAA
Tsp45I 1198 GTCAC ^GT[GC]AC
Tsp509I 821 AATT ^AATT
Tth111I 343 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 8 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1184 CCATCCTGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 809 AGATCT [GA]^GATC[CT]
XmnI 423 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 3 GGCGTC G[GA]^CG[CT]C
378 GGCGTC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
378 GGCGTC
BsiEI 159 CGGCCG CG[GA][CT]^CG
201 CGGCCG
BsmBI 402 CGTCTC CGTCTC(1/5)
990 CGTCTC
BsmFI 504 GGGAC GGGAC(10/14)
1208 GGGAC
BspMI 1139 ACCTGC ACCTGC(4/8)
1191 GCAGGT
BsrBI 610 CCGCTC CCGCTC(-3/-3)
1089 CCGCTC
BsrDI 657 CATTGC GCAATG(2/0)
1233 CATTGC
BsrI 410 CCAGT ACTGG(1/-1)
1259 CCAGT
CfoI 804 GCGC GCG^C
1068 GCGC
EagI 159 CGGCCG C^GGCCG
201 CGGCCG
EclXI 159 CGGCCG C^GGCCG
201 CGGCCG
Eco52I 159 CGGCCG C^GGCCG
201 CGGCCG
EcoNI 66 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
339 CCTGGACCAGG
EcoT14I 353 CCAAGG C^C[AT][AT]GG
998 CCAAGG
HhaI 804 GCGC GCG^C
1068 GCGC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
378 GGCGTC
HinP1I 804 GCGC G^CGC
1068 GCGC
HinfI 249 GACTC G^A[ACGT]TC
1210 GACTC
MslI 699 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CACCAAGGTG
MspA1I 12 CAGCTG C[AC]G^C[GT]G
620 CAGCTG
PleI 249 GACTC GAGTC(4/5)
1210 GACTC
PstI 418 CTGCAG CTGCA^G
1189 CTGCAG
PvuII 12 CAGCTG CAG^CTG
620 CAGCTG
SexAI 314 ACCAGGT A^CC[AT]GGT
344 ACCAGGT
SfaNI 88 GATGC GCATC(5/9)
323 GCATC
SfcI 418 CTGCAG C^T[GA][CT]AG
1189 CTGCAG
StyI 353 CCAAGG C^C[AT][AT]GG
998 CCAAGG
TaqI 116 TCGA T^CGA
1247 TCGA
TspRI 411 CAGTG CA[GC]TG[ACGT][ACGT]^
1260 CAGTG
TthHB8I 116 TCGA T^CGA
1247 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SmaI TseI XmaCI XmaI