Restriction map for
pFN21AE2798
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HhaI HinP1I HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp45I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 466 GTCGAC GT^[AC][GT]AC
AcyI 256 GGCGTC G[GA]^CG[CT]C
AlwNI 1627 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 198 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 680 GGATCC G^GATCC
BanI 1543 GGTGCC G^G[CT][GA]CC
BbsI 1201 GAAGAC GAAGAC(2/6)
BcgI 468 CGACAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 117 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 117 GCTGAGC GC^T[ACGT]AGC
BpuAI 1201 GAAGAC GAAGAC(2/6)
BsaHI 256 GGCGTC G[GA]^CG[CT]C
BseRI 1746 GAGGAG GAGGAG(10/8)
BsmI 1584 GAATGC GAATGC(1/-1)
BspMI 1185 GCAGGT ACCTGC(4/8)
BsrDI 122 GCAATG GCAATG(2/0)
BssSI 1345 CACGAG CACGAG(-5/-1)
CelII 117 GCTGAGC GC^T[ACGT]AGC
EcoNI 1395 CCTTGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
Hin1I 256 GGCGTC G[GA]^CG[CT]C
MaeII 12 ACGT A^CGT
MseI 1127 TTAA T^TAA
MspA1I 1627 CAGCTG C[AC]G^C[GT]G
NsiI 1 ATGCAT ATGCA^T
NspI 177 GCATGC [GA]CATG^[CT]
PflMI 273 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1627 CAGCTG CAG^CTG
SalI 466 GTCGAC G^TCGAC
SphI 177 GCATGC GCATG^C
TaqI 467 TCGA T^CGA
TfiI 170 GAATC G^A[AT]TC
Tru9I 1127 TTAA T^TAA
TthHB8I 467 TCGA T^CGA
Van91I 273 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 198 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BsrFI 235 ACCGGC [GA]^CCGG[CT]
694 GCCGGT
BstF5I 61 GGATG GGATG(2/0)
153 GGATG
BstYI 680 GGATCC [GA]^GATC[CT]
1654 GGATCT
Cfr10I 235 ACCGGC [GA]^CCGG[CT]
694 GCCGGT
FokI 61 GGATG GGATG(9/13)
153 GGATG
HgaI 257 GCGTC GACGC(5/10)
1751 GCGTC
HindIII 340 AAGCTT A^AGCTT
1531 AAGCTT
HphI 1278 TCACC GGTGA(8/7)
1386 TCACC
MboII 1201 GAAGA GAAGA(8/7)
1657 TCTTC
MflI 680 GGATCC [GA]^GATC[CT]
1654 GGATCT
SfaNI 3 GCATC GCATC(5/9)
158 GCATC
StuI 1133 AGGCCT AGG^CCT
1218 AGGCCT
XhoII 680 GGATCC [GA]^GATC[CT]
1654 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaII
BalI BanII BbvI BcnI BglI
BmyI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HincII HindII HinfI HpaII
ItaI MboI MluNI MnlI MscI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfcI StyI
TseI TspRI XcmI