Restriction map for
pFN21AE3363
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspI AvrII BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsgI BsiEI BsmBI
BspDI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 2528 TCCGGA T^CCGGA
AcyI 137 GGCGCC G[GA]^CG[CT]C
AflIII 183 ACATGT A^C[GA][CT]GT
AhdI 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1397 TGCGCA TGC^GCA
BamHI 16 GGATCC G^GATCC
BbeI 137 GGCGCC GGCGC^C
BsaHI 137 GGCGCC G[GA]^CG[CT]C
BsaWI 2528 TCCGGA [AT]^CCGG[AT]
BseAI 2528 TCCGGA T^CCGGA
BsiWI 1798 CGTACG C^GTACG
BsmI 2239 GAATGC GAATGC(1/-1)
Bsp1407I 2864 TGTACA T^GTACA
BspEI 2528 TCCGGA T^CCGGA
BspLU11I 183 ACATGT A^CATGT
BsrGI 2864 TGTACA T^GTACA
BssSI 1120 CACGAG CACGAG(-5/-1)
BstAPI 2000 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I 1202 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1397 TGCGCA TGC^GCA
Hin1I 137 GGCGCC G[GA]^CG[CT]C
HincII 1210 GTCAAC GT[CT]^[GA]AC
HindII 1210 GTCAAC GT[CT]^[GA]AC
HindIII 666 AAGCTT A^AGCTT
KasI 137 GGCGCC G^GCGCC
KspI 2327 CCGCGG CCGC^GG
MroI 2528 TCCGGA T^CCGGA
MseI 1601 TTAA T^TAA
NaeI 1459 GCCGGC GCC^GGC
NarI 137 GGCGCC GG^CGCC
NgoMIV 1459 GCCGGC G^CCGGC
PaeR7I 1682 CTCGAG C^TCGAG
SacII 2327 CCGCGG CCGC^GG
SapI 2487 GAAGAGC GCTCTTC(1/4)
SfoI 137 GGCGCC GGC^GCC
SmaI 1673 CCCGGG CCC^GGG
SspBI 2864 TGTACA T^GTACA
SspI 1138 AATATT AAT^ATT
StuI 2894 AGGCCT AGG^CCT
Tru9I 1601 TTAA T^TAA
Tsp509I 2687 AATT ^AATT
XhoI 1682 CTCGAG C^TCGAG
XmaCI 1673 CCCGGG C^CCGGG
XmaI 1673 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 79 GGTACC G^GTACC
1433 GGTACC
AccI 1105 GTCTAC GT^[AC][GT]AC
1162 GTCTAC
AocI 1966 CCTGAGG CC^T[ACGT]AGG
2658 CCTGAGG
Aor51HI 2183 AGCGCT AGC^GCT
3122 AGCGCT
Asp718I 79 GGTACC G^GTACC
1433 GGTACC
AspHI 1069 GAGCAC G[AT]GC[AT]^C
2755 GAGCAC
BlpI 48 GCTCAGC GC^T[ACGT]AGC
2602 GCTGAGC
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
2602 GCTGAGC
BsaBI 1347 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1920 GATGCACATC
BsiHKAI 1069 GAGCAC G[AT]GC[AT]^C
2755 GAGCAC
BsrBI 226 GAGCGG CCGCTC(-3/-3)
544 GAGCGG
Bsu36I 1966 CCTGAGG CC^T[ACGT]AGG
2658 CCTGAGG
CelII 48 GCTCAGC GC^T[ACGT]AGC
2602 GCTGAGC
EarI 2409 GAAGAG CTCTTC(1/4)
2487 GAAGAG
Eco47III 2183 AGCGCT AGC^GCT
3122 AGCGCT
Eco81I 1966 CCTGAGG CC^T[ACGT]AGG
2658 CCTGAGG
KpnI 79 GGTACC GGTAC^C
1433 GGTACC
Ksp632I 2409 GAAGAG CTCTTC(1/4)
2487 GAAGAG
MamI 1347 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1920 GATGCACATC
NcoI 1095 CCATGG C^CATGG
3018 CCATGG
NspI 183 ACATGT [GA]CATG^[CT]
971 ACATGC
PshAI 429 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
864 GACCCAGGTC
ScaI 2876 AGTACT AGT^ACT
3042 AGTACT
TfiI 1737 GATTC G^A[AT]TC
1866 GATTC
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII