Restriction map for
pFN21AE2782
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BsaBI BsaI
BsiEI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 343 GTAGAC GT^[AC][GT]AC
AccIII 1058 TCCGGA T^CCGGA
AcsI 22 GAATTT [GA]^AATT[CT]
AcyI 286 GGCGTC G[GA]^CG[CT]C
ApoI 22 GAATTT [GA]^AATT[CT]
AspI 819 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 133 CTCGGG C^[CT]CG[GA]G
BamHI 1076 GGATCC G^GATCC
BcgI 1125 GCACCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 83 AGATCT A^GATCT
BpmI 126 CTGGAG CTGGAG(16/14)
BsaAI 1042 TACGTG [CT]AC^GT[GA]
BsaHI 286 GGCGTC G[GA]^CG[CT]C
BsaWI 1058 TCCGGA [AT]^CCGG[AT]
BseAI 1058 TCCGGA T^CCGGA
BsgI 208 CTGCAC GTGCAG(16/14)
BsmBI 1339 CGTCTC CGTCTC(1/5)
BsmI 924 GAATGC GAATGC(1/-1)
BsoBI 133 CTCGGG C^[CT]CG[GA]G
BspEI 1058 TCCGGA T^CCGGA
BspMI 884 ACCTGC ACCTGC(4/8)
BsrFI 560 GCCGGT [GA]^CCGG[CT]
BssSI 250 CTCGTG CACGAG(-5/-1)
BstXI 620 CCACTTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 560 GCCGGT [GA]^CCGG[CT]
EarI 640 CTCTTC CTCTTC(1/4)
EcoRV 510 GATATC GAT^ATC
EcoT14I 540 CCAAGG C^C[AT][AT]GG
HgaI 287 GCGTC GACGC(5/10)
Hin1I 286 GGCGTC G[GA]^CG[CT]C
Ksp632I 640 CTCTTC CTCTTC(1/4)
MroI 1058 TCCGGA T^CCGGA
MseI 1159 TTAA T^TAA
PflFI 819 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1301 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 568 CTGCAG CTGCA^G
SfcI 568 CTGCAG C^T[GA][CT]AG
SphI 156 GCATGC GCATG^C
StyI 540 CCAAGG C^C[AT][AT]GG
Tru9I 1159 TTAA T^TAA
Tsp509I 23 AATT ^AATT
Tth111I 819 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1301 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 440 GTAC GT^AC
648 GTAC
ApaI 502 GGGCCC GGGCC^C
630 GGGCCC
AspHI 1175 GAGCTC G[AT]GC[AT]^C
1288 GAGCTC
BbsI 289 GTCTTC GAAGAC(2/6)
496 GTCTTC
BcnI 426 CCGGG CC^[GC]GG
581 CCCGG
BglI 391 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
763 GCCACGTTGGC
BlpI 231 GCTCAGC GC^T[ACGT]AGC
1290 GCTCAGC
Bpu1102I 231 GCTCAGC GC^T[ACGT]AGC
1290 GCTCAGC
BpuAI 289 GTCTTC GAAGAC(2/6)
496 GTCTTC
BsiHKAI 1175 GAGCTC G[AT]GC[AT]^C
1288 GAGCTC
BstAPI 33 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
360 GCACAAGATGC
BstEII 667 GGTGACC G^GT[ACGT]ACC
960 GGTCACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
960 GGTCACC
CelII 231 GCTCAGC GC^T[ACGT]AGC
1290 GCTCAGC
CfoI 977 GCGC GCG^C
1065 GCGC
DsaI 167 CCGTGG C^C[GA][CT]GG
758 CCACGG
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
960 GGTCACC
HaeII 976 AGCGCC [GA]GCGC^[CT]
1064 AGCGCC
HhaI 977 GCGC GCG^C
1065 GCGC
HinP1I 977 GCGC G^CGC
1065 GCGC
MaeII 766 ACGT A^CGT
1043 ACGT
MslI 621 CACTTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1038 CACTTACGTG
MspA1I 205 CAGCTG C[AC]G^C[GT]G
1293 CAGCTG
NciI 426 CCGGG CC^[GC]GG
581 CCCGG
NspI 156 GCATGC [GA]CATG^[CT]
1050 GCATGT
PpuMI 812 GGGACCT [GA]G^G[AT]CC[CT]
940 GGGACCT
PvuII 205 CAGCTG CAG^CTG
1293 CAGCTG
RsaI 440 GTAC GT^AC
648 GTAC
SacI 1175 GAGCTC GAGCT^C
1288 GAGCTC
XcmI 758 CCACGGCCACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCATCTTCCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BanI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII