Restriction map for
pFN21AE2669
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BbeI BbrPI
BbvCI BclI BcnI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Cac8I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 866 GTAGAC GT^[AC][GT]AC
AcsI 967 AAATTC [GA]^AATT[CT]
AfaI 127 GTAC GT^AC
AocI 1055 CCTTAGG CC^T[ACGT]AGG
ApoI 967 AAATTC [GA]^AATT[CT]
BamHI 936 GGATCC G^GATCC
BanII 403 GGGCTC G[GA]GC[CT]^C
BcgI 556 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 35 GTATCC GTATCC(6/5)
BglI 597 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 94 AGATCT A^GATCT
BsaJI 322 CCCTGG C^C[ACGT][ACGT]GG
BseRI 222 CTCCTC GAGGAG(10/8)
BsrBI 100 GAGCGG CCGCTC(-3/-3)
BsrDI 61 GCAATG GCAATG(2/0)
BsrFI 169 ACCGGC [GA]^CCGG[CT]
BstXI 377 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1055 CCTTAGG CC^T[ACGT]AGG
Cfr10I 169 ACCGGC [GA]^CCGG[CT]
Cfr13I 595 GGGCC G^G[ACGT]CC
DraII 594 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 260 TGGCCG [CT]^GGCC[GA]
Eco81I 1055 CCTTAGG CC^T[ACGT]AGG
EcoNI 39 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 594 GGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 170 CCGG C^CGG
HpaII 170 CCGG C^CGG
MaeIII 49 GTGAC ^GT[ACGT]AC
MspA1I 130 CAGCTG C[AC]G^C[GT]G
MspI 170 CCGG C^CGG
NdeI 633 CATATG CA^TATG
PleI 307 GACTC GAGTC(4/5)
PvuII 130 CAGCTG CAG^CTG
RsaI 127 GTAC GT^AC
Sau96I 595 GGGCC G^G[ACGT]CC
SfcI 864 CTGTAG C^T[GA][CT]AG
SspI 451 AATATT AAT^ATT
Tsp45I 49 GTGAC ^GT[GC]AC
Tsp509I 968 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 8 CCGC CCGC(-3/-1)
102 GCGG
AlwI 937 GATCC GGATC(4/5)
936 GGATC
BanI 104 GGTGCC G^G[CT][GA]CC
318 GGCACC
BbsI 17 GAAGAC GAAGAC(2/6)
787 GTCTTC
BbvI 327 GCAGC GCAGC(8/12)
916 GCAGC
BmyI 105 GTGCCC G[AGT]GC[ACT]^C
403 GGGCTC
BpuAI 17 GAAGAC GAAGAC(2/6)
787 GTCTTC
BsaI 373 GAGACC GGTCTC(1/5)
486 GGTCTC
BsiYI 39 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCTTAAAGGGG
BslI 39 CCTCCCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCTTAAAGGGG
Bsp1286I 105 GTGCCC G[AGT]GC[ACT]^C
403 GGGCTC
BsrI 54 ACTGG ACTGG(1/-1)
639 CCAGT
BssKI 315 CCTGG ^CC[ACGT]GG
323 CCTGG
BstF5I 174 CATCC GGATG(2/0)
213 CATCC
BstNI 315 CCTGG CC^[AT]GG
323 CCTGG
BstYI 94 AGATCT [GA]^GATC[CT]
936 GGATCC
EcoRII 315 CCTGG ^CC[AT]GG
323 CCTGG
FokI 174 CATCC GGATG(9/13)
213 CATCC
HaeIII 261 GGCC GG^CC
596 GGCC
HphI 48 GGTGA GGTGA(8/7)
360 GGTGA
MflI 94 AGATCT [GA]^GATC[CT]
936 GGATCC
MseI 589 TTAA T^TAA
943 TTAA
MvaI 315 CCTGG CC^[AT]GG
323 CCTGG
ScrFI 315 CCTGG CC^[ACGT]GG
323 CCTGG
SmlI 427 CTCAAG C^T[CT][GA]AG
540 CTTGAG
TfiI 422 GATTC G^A[AT]TC
709 GATTC
Tru9I 589 TTAA T^TAA
943 TTAA
TseI 327 GCAGC G^C[AT]GC
916 GCAGC
XhoII 94 AGATCT [GA]^GATC[CT]
936 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BsmAI DdeI DpnII Fnu4HI
HinfI ItaI MboI MboII MnlI
MslI MwoI NdeII NlaIII NlaIV
Sau3AI SfaNI TspRI