Restriction map for
pFN21AE2713
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 445 AAATTT [GA]^AATT[CT]
ApoI 445 AAATTT [GA]^AATT[CT]
BamHI 141 GGATCC G^GATCC
BanI 768 GGTGCC G^G[CT][GA]CC
BanII 182 GAGCCC G[GA]GC[CT]^C
BfaI 853 CTAG C^TAG
BmyI 182 GAGCCC G[AGT]GC[ACT]^C
BsmFI 32 GGGAC GGGAC(10/14)
Bsp1286I 182 GAGCCC G[AGT]GC[ACT]^C
BspHI 591 TCATGA T^CATGA
BsrDI 757 GCAATG GCAATG(2/0)
BstEII 267 GGTTACC G^GT[ACGT]ACC
BstPI 267 GGTTACC G^GT[ACGT]ACC
Cac8I 510 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 902 GCGC GCG^C
DraI 256 TTTAAA TTT^AAA
DraIII 930 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 454 CCACGG C^C[GA][CT]GG
EarI 429 GAAGAG CTCTTC(1/4)
EcoO65I 267 GGTTACC G^GT[ACGT]ACC
EcoT14I 263 CCAAGG C^C[AT][AT]GG
HhaI 902 GCGC GCG^C
HinP1I 902 GCGC G^CGC
HphI 839 TCACC GGTGA(8/7)
Ksp632I 429 GAAGAG CTCTTC(1/4)
MaeI 853 CTAG C^TAG
PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 629 CAGCTG CAG^CTG
RcaI 591 TCATGA T^CATGA
SexAI 931 ACCAGGT A^CC[AT]GGT
SfaNI 229 GATGC GCATC(5/9)
StyI 263 CCAAGG C^C[AT][AT]GG
SwaI 255 ATTTAAAT ATTT^AAAT
Tsp45I 482 GTGAC ^GT[GC]AC
TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 749 CCATATCAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 326 ACGTGT A^C[GA][CT]GT
527 ACACGT
AvaII 33 GGACC G^G[AT]CC
503 GGACC
BcnI 30 CCGGG CC^[GC]GG
710 CCCGG
BsmAI 21 GAGAC GTCTC(1/5)
394 GAGAC
BsmBI 21 GAGACG CGTCTC(1/5)
394 GAGACG
BsrI 84 CCAGT ACTGG(1/-1)
621 CCAGT
DdeI 114 CTCAG C^T[ACGT]AG
521 CTCAG
DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT]
512 AGGCCCC
EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT]
512 AGGCCCC
HaeIII 499 GGCC GG^CC
513 GGCC
MaeIII 268 GTTAC ^GT[ACGT]AC
482 GTGAC
MboII 426 GAAGA GAAGA(8/7)
429 GAAGA
MseI 257 TTAA T^TAA
443 TTAA
MspA1I 70 CAGCGG C[AC]G^C[GT]G
629 CAGCTG
NciI 30 CCGGG CC^[GC]GG
710 CCCGG
PleI 595 GACTC GAGTC(4/5)
155 GAGTC
TaqI 567 TCGA T^CGA
863 TCGA
TfiI 460 GATTC G^A[AT]TC
836 GAATC
Tru9I 257 TTAA T^TAA
443 TTAA
TthHB8I 567 TCGA T^CGA
863 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstYI Cfr13I DpnII EcoRII
FauI Fnu4HI FokI HapII HinfI
HpaII ItaI MaeII MboI MflI
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI Tsp509I XhoII