################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BlpI Bpu1102I BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstAPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRV FseI FspI HaeII HgaI HpaI KasI KpnI KspI MluI MroI NarI NcoI NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1343 GACGTC GACGT^C AccI 2165 GTCGAC GT^[AC][GT]AC AcyI 1343 GACGTC G[GA]^CG[CT]C AflIII 985 ACATGT A^C[GA][CT]GT AocI 3873 CCTTAGG CC^T[ACGT]AGG ApaI 136 GGGCCC GGGCC^C AvrII 351 CCTAGG C^CTAGG BanI 643 GGTGCC G^G[CT][GA]CC BbsI 3505 GAAGAC GAAGAC(2/6) BciVI 948 GTATCC GTATCC(6/5) BclI 1416 TGATCA T^GATCA BglI 40 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 270 AGATCT A^GATCT BlnI 351 CCTAGG C^CTAGG BpuAI 3505 GAAGAC GAAGAC(2/6) BsaAI 380 TACGTA [CT]AC^GT[GA] BsaBI 3708 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1343 GACGTC G[GA]^CG[CT]C BspLU11I 985 ACATGT A^CATGT BspMI 334 ACCTGC ACCTGC(4/8) BstBI 281 TTCGAA TT^CGAA BstEII 3007 GGTAACC G^GT[ACGT]ACC BstPI 3007 GGTAACC G^GT[ACGT]ACC Bsu36I 3873 CCTTAGG CC^T[ACGT]AGG DraIII 1135 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 140 CCGTGG C^C[GA][CT]GG Eco81I 3873 CCTTAGG CC^T[ACGT]AGG EcoNI 2997 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3007 GGTAACC G^GT[ACGT]ACC EcoT22I 824 ATGCAT ATGCA^T FbaI 1416 TGATCA T^GATCA Hin1I 1343 GACGTC G[GA]^CG[CT]C HincII 2165 GTCGAC GT[CT]^[GA]AC HindII 2165 GTCGAC GT[CT]^[GA]AC MamI 3708 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3836 CAATTG C^AATTG MspA1I 1828 CAGCTG C[AC]G^C[GT]G MunI 3836 CAATTG C^AATTG NaeI 95 GCCGGC GCC^GGC NgoMIV 95 GCCGGC G^CCGGC NsiI 824 ATGCAT ATGCA^T NspV 281 TTCGAA TT^CGAA PaeR7I 1721 CTCGAG C^TCGAG PshAI 3426 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1828 CAGCTG CAG^CTG SalI 2165 GTCGAC G^TCGAC SfuI 281 TTCGAA TT^CGAA SmaI 90 CCCGGG CCC^GGG SnaBI 380 TACGTA TAC^GTA StuI 2256 AGGCCT AGG^CCT XcmI 2370 CCACATGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1721 CTCGAG C^TCGAG XmaCI 90 CCCGGG C^CCGGG XmaI 90 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 862 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 3092 CAGAACCTG AspHI 2750 GAGCTC G[AT]GC[AT]^C 3812 GAGCTC AvaI 90 CCCGGG C^[CT]CG[GA]G 1721 CTCGAG BsaI 2861 GAGACC GGTCTC(1/5) 3280 GAGACC BsiHKAI 2750 GAGCTC G[AT]GC[AT]^C 3812 GAGCTC BsmFI 449 GGGAC GGGAC(10/14) 4108 GTCCC BsoBI 90 CCCGGG C^[CT]CG[GA]G 1721 CTCGAG Bsp1407I 1691 TGTACA T^GTACA 2490 TGTACA BspHI 2574 TCATGA T^CATGA 3971 TCATGA BsrFI 95 GCCGGC [GA]^CCGG[CT] 2242 ACCGGC BsrGI 1691 TGTACA T^GTACA 2490 TGTACA BssSI 1472 CACGAG CACGAG(-5/-1) 2963 CTCGTG Cfr10I 95 GCCGGC [GA]^CCGG[CT] 2242 ACCGGC EcoRI 3069 GAATTC G^AATTC 3879 GAATTC NdeI 827 CATATG CA^TATG 3152 CATATG PflMI 1824 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3438 CCAGGCATTGG PstI 1219 CTGCAG CTGCA^G 1609 CTGCAG RcaI 2574 TCATGA T^CATGA 3971 TCATGA SacI 2750 GAGCTC GAGCT^C 3812 GAGCTC SspBI 1691 TGTACA T^GTACA 2490 TGTACA Van91I 1824 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3438 CCAGGCATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI