Restriction map for
pFN21AE2688
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BbeI BbrPI BbvCI
BcgI BfrI BlpI Bpu1102I BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BssHII Bst1107I BstAPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRV
FseI FspI HaeII HgaI HpaI
KasI KpnI KspI MluI MroI
NarI NcoI NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SpeI
Sse8387I SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1343 GACGTC GACGT^C
AccI 2165 GTCGAC GT^[AC][GT]AC
AcyI 1343 GACGTC G[GA]^CG[CT]C
AflIII 985 ACATGT A^C[GA][CT]GT
AocI 3873 CCTTAGG CC^T[ACGT]AGG
ApaI 136 GGGCCC GGGCC^C
AvrII 351 CCTAGG C^CTAGG
BanI 643 GGTGCC G^G[CT][GA]CC
BbsI 3505 GAAGAC GAAGAC(2/6)
BciVI 948 GTATCC GTATCC(6/5)
BclI 1416 TGATCA T^GATCA
BglI 40 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 270 AGATCT A^GATCT
BlnI 351 CCTAGG C^CTAGG
BpuAI 3505 GAAGAC GAAGAC(2/6)
BsaAI 380 TACGTA [CT]AC^GT[GA]
BsaBI 3708 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1343 GACGTC G[GA]^CG[CT]C
BspLU11I 985 ACATGT A^CATGT
BspMI 334 ACCTGC ACCTGC(4/8)
BstBI 281 TTCGAA TT^CGAA
BstEII 3007 GGTAACC G^GT[ACGT]ACC
BstPI 3007 GGTAACC G^GT[ACGT]ACC
Bsu36I 3873 CCTTAGG CC^T[ACGT]AGG
DraIII 1135 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 140 CCGTGG C^C[GA][CT]GG
Eco81I 3873 CCTTAGG CC^T[ACGT]AGG
EcoNI 2997 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3007 GGTAACC G^GT[ACGT]ACC
EcoT22I 824 ATGCAT ATGCA^T
FbaI 1416 TGATCA T^GATCA
Hin1I 1343 GACGTC G[GA]^CG[CT]C
HincII 2165 GTCGAC GT[CT]^[GA]AC
HindII 2165 GTCGAC GT[CT]^[GA]AC
MamI 3708 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3836 CAATTG C^AATTG
MspA1I 1828 CAGCTG C[AC]G^C[GT]G
MunI 3836 CAATTG C^AATTG
NaeI 95 GCCGGC GCC^GGC
NgoMIV 95 GCCGGC G^CCGGC
NsiI 824 ATGCAT ATGCA^T
NspV 281 TTCGAA TT^CGAA
PaeR7I 1721 CTCGAG C^TCGAG
PshAI 3426 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1828 CAGCTG CAG^CTG
SalI 2165 GTCGAC G^TCGAC
SfuI 281 TTCGAA TT^CGAA
SmaI 90 CCCGGG CCC^GGG
SnaBI 380 TACGTA TAC^GTA
StuI 2256 AGGCCT AGG^CCT
XcmI 2370 CCACATGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1721 CTCGAG C^TCGAG
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 862 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
3092 CAGAACCTG
AspHI 2750 GAGCTC G[AT]GC[AT]^C
3812 GAGCTC
AvaI 90 CCCGGG C^[CT]CG[GA]G
1721 CTCGAG
BsaI 2861 GAGACC GGTCTC(1/5)
3280 GAGACC
BsiHKAI 2750 GAGCTC G[AT]GC[AT]^C
3812 GAGCTC
BsmFI 449 GGGAC GGGAC(10/14)
4108 GTCCC
BsoBI 90 CCCGGG C^[CT]CG[GA]G
1721 CTCGAG
Bsp1407I 1691 TGTACA T^GTACA
2490 TGTACA
BspHI 2574 TCATGA T^CATGA
3971 TCATGA
BsrFI 95 GCCGGC [GA]^CCGG[CT]
2242 ACCGGC
BsrGI 1691 TGTACA T^GTACA
2490 TGTACA
BssSI 1472 CACGAG CACGAG(-5/-1)
2963 CTCGTG
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
2242 ACCGGC
EcoRI 3069 GAATTC G^AATTC
3879 GAATTC
NdeI 827 CATATG CA^TATG
3152 CATATG
PflMI 1824 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3438 CCAGGCATTGG
PstI 1219 CTGCAG CTGCA^G
1609 CTGCAG
RcaI 2574 TCATGA T^CATGA
3971 TCATGA
SacI 2750 GAGCTC GAGCT^C
3812 GAGCTC
SspBI 1691 TGTACA T^GTACA
2490 TGTACA
Van91I 1824 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3438 CCAGGCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI