Restriction map for
pFN21AE2704
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccII AccIII AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaAI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT22I
FseI FspI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AclI 376 AACGTT AA^CGTT
AocI 3959 CCTCAGG CC^T[ACGT]AGG
AseI 301 ATTAAT AT^TAAT
AsnI 301 ATTAAT AT^TAAT
BalI 3740 TGGCCA TGG^CCA
BamHI 3455 GGATCC G^GATCC
BanI 716 GGCACC G^G[CT][GA]CC
BclI 3878 TGATCA T^GATCA
BglI 3570 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4019 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1697 ACCGGA [AT]^CCGG[AT]
BsgI 1868 CTGCAC GTGCAG(16/14)
BspMI 2215 GCAGGT ACCTGC(4/8)
BsrDI 2528 GCAATG GCAATG(2/0)
BssSI 1667 CTCGTG CACGAG(-5/-1)
BstAPI 4001 GCAACGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3959 CCTCAGG CC^T[ACGT]AGG
DraIII 2344 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2318 CCATGG C^C[GA][CT]GG
Eco81I 3959 CCTCAGG CC^T[ACGT]AGG
EcoNI 2727 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 790 GATATC GAT^ATC
FauI 3150 GCGGG CCCGC(4/6)
FbaI 3878 TGATCA T^GATCA
HpaI 1719 GTTAAC GTT^AAC
MamI 4019 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3740 TGGCCA TGG^CCA
MscI 3740 TGGCCA TGG^CCA
NcoI 2318 CCATGG C^CATGG
NdeI 3834 CATATG CA^TATG
PaeR7I 762 CTCGAG C^TCGAG
PflMI 3554 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3622 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 301 ATTAAT AT^TAAT
Psp1406I 376 AACGTT AA^CGTT
ScaI 2816 AGTACT AGT^ACT
StuI 3184 AGGCCT AGG^CCT
Van91I 3554 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 270 TCTAGA T^CTAGA
XcmI 3728 CCACCACAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 762 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 319 GGCGTC G[GA]^CG[CT]C
2139 GACGCC
AlwNI 156 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3962 CAGGAGCTG
AspI 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1455 GACTGTGTC
BcnI 1035 CCCGG CC^[GC]GG
1878 CCCGG
BsaHI 319 GGCGTC G[GA]^CG[CT]C
2139 GACGCC
BsrBI 2686 GAGCGG CCGCTC(-3/-3)
3148 GAGCGG
EaeI 3740 TGGCCA [CT]^GGCC[GA]
4033 CGGCCA
EcoRI 2513 GAATTC G^AATTC
2808 GAATTC
HaeII 2363 GGCGCT [GA]GCGC^[CT]
3131 AGCGCC
Hin1I 319 GGCGTC G[GA]^CG[CT]C
2139 GACGCC
HincII 1719 GTTAAC GT[CT]^[GA]AC
3478 GTCAAC
HindII 1719 GTTAAC GT[CT]^[GA]AC
3478 GTCAAC
NciI 1035 CCCGG CC^[GC]GG
1878 CCCGG
NspI 2855 GCATGT [GA]CATG^[CT]
3671 ACATGC
PflFI 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1455 GACTGTGTC
SacI 76 GAGCTC GAGCT^C
83 GAGCTC
SapI 3064 GAAGAGC GCTCTTC(1/4)
3953 GCTCTTC
SspI 2488 AATATT AAT^ATT
3314 AATATT
Tth111I 154 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1455 GACTGTGTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
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AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI