Restriction map for
pFN21AE2750
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# NO CUT ENZYMES ( 50 enzymes) #
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AatII AflII Alw44I AocI ApaLI
AscI AseI AsnI AspI AviII
AvrII BfrI BlnI Bsp1407I BspDI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI Eco81I EcoT22I
FspI HpaI MfeI MunI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshBI PvuI RsrII
SbfI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 29 enzymes) #
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Acc65I 5443 GGTACC G^GTACC
AgeI 2662 ACCGGT A^CCGGT
Aor51HI 262 AGCGCT AGC^GCT
Asp718I 5443 GGTACC G^GTACC
BbeI 14 GGCGCC GGCGC^C
BglII 3275 AGATCT A^GATCT
BsiWI 5564 CGTACG C^GTACG
BspHI 4113 TCATGA T^CATGA
BssHII 4611 GCGCGC G^CGCGC
DraI 2109 TTTAAA TTT^AAA
DrdI 4249 GACTCTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 262 AGCGCT AGC^GCT
EcoRI 3824 GAATTC G^AATTC
EcoRV 2842 GATATC GAT^ATC
FseI 167 GGCCGGCC GGCCGG^CC
KasI 14 GGCGCC G^GCGCC
KpnI 5443 GGTACC GGTAC^C
KspI 4623 CCGCGG CCGC^GG
MluI 2522 ACGCGT A^CGCGT
NarI 14 GGCGCC GG^CGCC
NheI 4432 GCTAGC G^CTAGC
PaeR7I 2179 CTCGAG C^TCGAG
PinAI 2662 ACCGGT A^CCGGT
PshAI 2370 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4113 TCATGA T^CATGA
SacII 4623 CCGCGG CCGC^GG
SfoI 14 GGCGCC GGC^GCC
SgrAI 2661 CACCGGTG C[GA]^CCGG[CT]G
XhoI 2179 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccIII 198 TCCGGA T^CCGGA
3873 TCCGGA
AclI 1820 AACGTT AA^CGTT
4787 AACGTT
AhdI 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4651 GACGACCTGTC
AspEI 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4651 GACGACCTGTC
BamHI 2539 GGATCC G^GATCC
4932 GGATCC
BbrPI 2461 CACGTG CAC^GTG
4402 CACGTG
BcgI 5076 CGACGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1666 GCATTTTTCTCG
BclI 2513 TGATCA T^GATCA
2867 TGATCA
BsaI 1751 GAGACC GGTCTC(1/5)
918 GGTCTC
BseAI 198 TCCGGA T^CCGGA
3873 TCCGGA
BsmI 1057 GCATTC GAATGC(1/-1)
1688 GCATTC
BspEI 198 TCCGGA T^CCGGA
3873 TCCGGA
BsrBI 81 GAGCGG CCGCTC(-3/-3)
4341 GAGCGG
BstAPI 1048 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2293 GCAGTCACTGC
BstEII 3817 GGTTACC G^GT[ACGT]ACC
5677 GGTTACC
BstPI 3817 GGTTACC G^GT[ACGT]ACC
5677 GGTTACC
Eam1105I 1463 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4651 GACGACCTGTC
EcoO65I 3817 GGTTACC G^GT[ACGT]ACC
5677 GGTTACC
FbaI 2513 TGATCA T^GATCA
2867 TGATCA
HindIII 2160 AAGCTT A^AGCTT
3061 AAGCTT
MroI 198 TCCGGA T^CCGGA
3873 TCCGGA
NdeI 662 CATATG CA^TATG
2831 CATATG
PmaCI 2461 CACGTG CAC^GTG
4402 CACGTG
PmlI 2461 CACGTG CAC^GTG
4402 CACGTG
Psp1406I 1820 AACGTT AA^CGTT
4787 AACGTT
PvuII 2044 CAGCTG CAG^CTG
3592 CAGCTG
SalI 2797 GTCGAC G^TCGAC
3850 GTCGAC
SapI 2888 GAAGAGC GCTCTTC(1/4)
4770 GAAGAGC
ScaI 2928 AGTACT AGT^ACT
3144 AGTACT
SexAI 4814 ACCAGGT A^CC[AT]GGT
5295 ACCAGGT
SfiI 162 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5549 GGCCAGGCTGGCC
SmaI 72 CCCGGG CCC^GGG
123 CCCGGG
StuI 3210 AGGCCT AGG^CCT
5360 AGGCCT
XbaI 1483 TCTAGA T^CTAGA
2451 TCTAGA
XmaCI 72 CCCGGG C^CCGGG
123 CCCGGG
XmaI 72 CCCGGG C^CCGGG
123 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI