Restriction map for
pFN21AE2685
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbrPI BbvCI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsgI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 419 TCCGGA T^CCGGA
AcsI 1288 AAATTC [GA]^AATT[CT]
AflIII 1134 ACGTGT A^C[GA][CT]GT
AlwNI 898 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1288 AAATTC [GA]^AATT[CT]
Asp700I 1149 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 647 CGATCCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 446 TGATCA T^GATCA
BfaI 1123 CTAG C^TAG
BglI 152 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 419 TCCGGA T^CCGGA
BseRI 20 CTCCTC GAGGAG(10/8)
BsmI 818 GCATTC GAATGC(1/-1)
BspDI 970 ATCGAT AT^CGAT
BspEI 419 TCCGGA T^CCGGA
BsrFI 90 GCCGGC [GA]^CCGG[CT]
BstBI 992 TTCGAA TT^CGAA
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
ClaI 970 ATCGAT AT^CGAT
DraII 625 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1261 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1354 GAAGAG CTCTTC(1/4)
EcoO109I 625 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 446 TGATCA T^GATCA
HgaI 409 GACGC GACGC(5/10)
Ksp632I 1354 GAAGAG CTCTTC(1/4)
MaeI 1123 CTAG C^TAG
MroI 419 TCCGGA T^CCGGA
NaeI 90 GCCGGC GCC^GGC
NgoMIV 90 GCCGGC G^CCGGC
NspV 992 TTCGAA TT^CGAA
PflFI 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 625 GGGACCC [GA]G^G[AT]CC[CT]
PstI 193 CTGCAG CTGCA^G
SfuI 992 TTCGAA TT^CGAA
SgrAI 89 CGCCGGCG C[GA]^CCGG[CT]G
StuI 1125 AGGCCT AGG^CCT
Tth111I 900 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1149 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AfaI 729 GTAC GT^AC
1111 GTAC
AluI 101 AGCT AG^CT
587 AGCT
Alw44I 114 GTGCAC G^TGCAC
655 GTGCAC
ApaLI 114 GTGCAC G^TGCAC
655 GTGCAC
AspHI 114 GTGCAC G[AT]GC[AT]^C
655 GTGCAC
BalI 830 TGGCCA TGG^CCA
1103 TGGCCA
BbsI 777 GAAGAC GAAGAC(2/6)
1201 GAAGAC
BpuAI 777 GAAGAC GAAGAC(2/6)
1201 GAAGAC
BsaWI 419 TCCGGA [AT]^CCGG[AT]
953 ACCGGA
BsiHKAI 114 GTGCAC G[AT]GC[AT]^C
655 GTGCAC
BsrBI 387 GAGCGG CCGCTC(-3/-3)
524 GAGCGG
BstXI 746 CCAAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1320 CCAAATGACTGG
MaeII 665 ACGT A^CGT
1134 ACGT
MluNI 830 TGGCCA TGG^CCA
1103 TGGCCA
MscI 830 TGGCCA TGG^CCA
1103 TGGCCA
MspA1I 304 CCGCTG C[AC]G^C[GT]G
427 CCGCTG
NcoI 770 CCATGG C^CATGG
827 CCATGG
NlaIII 771 CATG CATG^
828 CATG
RsaI 729 GTAC GT^AC
1111 GTAC
SfcI 147 CTACAG C^T[GA][CT]AG
193 CTGCAG
SmaI 361 CCCGGG CCC^GGG
404 CCCGGG
Tsp509I 179 AATT ^AATT
1289 AATT
XmaCI 361 CCCGGG C^CCGGG
404 CCCGGG
XmaI 361 CCCGGG C^CCGGG
404 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AlwI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII